Éditeur(s) : DIREN : Direction Régionale de l'Environnement Université des Antilles et de la Guyane DIREN : Direction Régionale de l'Environnement Université des Antilles et de la Guyane
Extrait de : Conférence Internationale "Biodiversité terrestre des Petites Antilles", 8-10 novembre 2010. Université des Antilles et de la Guyane, Faculté des Lettres et sciences humaines, campus de Schoelcher, Martinique.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Provenance : Université des Antilles et de la Guyane. Service commun de la documentation
Permalien : http://www.manioc.org/fichiers/HASH7e7ec5e949144ac2d0a970
HASH7e7ec5e949144ac2d0a970

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : DIREN : Direction Régionale de l'Environnement Université des Antilles et de la Guyane DIREN : Direction Régionale de l'Environnement Université des Antilles et de la Guyane

Droits : Document protégé par le droit d'auteur
fichiers:HASH7e7ec5e949144ac2d0a970

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : International audience
Sediments cored along the southwestern Iberian margin during Integrated Ocean Drilling Program Expedition 339 provide constraints on Mediterranean Outflow Water (MOW) circulation patterns from the Pliocene epoch to the present day. After the Strait of Gibraltar opened (5.33 million years ago), a limited volume of MOW entered the Atlantic. Depositional hiatuses indicate erosion by bottom currents related to higher volumes of MOW circulating into the North Atlantic, beginning in the late Pliocene. The hiatuses coincide with regional tectonic events and changes in global thermohaline circulation (THC). This suggests that MOW influenced Atlantic Meridional Overturning Circulation (AMOC), THC, and climatic shifts by contributing a component of warm, saline water to northern latitudes while in turn being influenced by plate tectonics.
ISSN: 0036-8075

hal-01054316
https://hal.archives-ouvertes.fr/hal-01054316
DOI : 10.1126/science.1251306

Éditeur(s) : HAL CCSD Universidad Complutense de Madrid
Résumé : The Lodève Permian synrift sequence is represented by thick stacked climate-controled playa cycles which have been constrained by structural, sedimentological, geochemical and paleontological processes. The present paper merges the different results in order to better constrain the dynamics and palaeoecologycal evolution of the playa system from Late Cisuralian to Early Lopingian time. In this depositional environment, thining upwards sheet-flood sequences represent flooding events; they are relayed by ephemeral ponding, suspension settling and limited carbonate precipitation accompanied by large amounts of shallow to subaerial sedimentary structures... (Ver más) (current and wind ripples, desiccation cracks, rain drops, etc.). The aridity of the climate contrasts with the surprising abundance of the fauna encountered into carbonate-rich silty-clay playa-lake deposits. Ephemeral pools are colonized by numerous shellfish: Choncostraca, Triopsids, and Insects, which led to intense burrowing of the overbank deposits (Scoyenia facies). Tetrapods are common and dominated by several types of reptiles after footprints: Pelycosauria, Parareptilia and Lepidosauria in the Rabejac Fm, and Mammalian reptiles (Therapsida) on the top of the fluvial deposits of the Salagou Fm near La Lieude farm. In addition, elements of a weighty herbivorous Pelycosaurian and a small amphibian Tupilakosaurid, only described in the Triassic before, have been also found in the Upper part of the Permian series of this sector.
ISSN: 1698-6180

hal-00411539
https://hal.archives-ouvertes.fr/hal-00411539

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : We present a preliminary probabilistic tsunami hazard assessment of Canadian coastlines from local and far-field, earthquake, and large submarine landslide sources. Analyses involve published historical, palaeotsunami and palaeoseismic data, modelling, and empirical relations between fault area, earthquake magnitude, and tsunami run-up. The cumulative estimated tsunami hazard for potentially damaging run-up (≥1.5 m) of the outer Pacific coastline is ~40-80 % in 50 years, respectively one and two orders of magnitude greater than the outer Atlantic (~1-15 %) and the Arctic (<1 %). For larger run-up with significant damage potential (≥3 m), Pacific hazard is ~10-30 % in 50 years, again much larger than both the Atlantic (~1-5 %) and Arctic (<1 %). For outer Pacific coastlines, the ≥1.5 m run-up hazard is dominated by far-field subduction zones, but the probability of run-up ≥3 m is highest for local megathrust sources, particularly the Cascadia subduction zone; thrust sources further north are also significant, as illustrated by the 2012 Haida Gwaii event. For Juan de Fuca and Georgia Straits, the Cascadia megathrust dominates the hazard at both levels. Tsunami hazard on the Atlantic coastline is dominated by poorly constrained far-field subduction sources; a lesser hazard is posed by near-field continental slope failures similar to the 1929 Grand Banks event. Tsunami hazard on the Arctic coastline is poorly constrained, but is likely dominated by continental slope failures; a hypothetical earthquake source beneath the Mackenzie delta requires further study. We highlight areas susceptible to locally damaging landslide-generated tsunamis, but do not quantify the hazard.
ISSN: 0921-030X

hal-00963614
https://hal.archives-ouvertes.fr/hal-00963614
DOI : 10.1007/s11069-013-0809-5