Manioc : Portail | resultats 1 - 3 pour la recherche: Guyomard-Rabenirina, Stéphanie

Temporal trends and risks factors for antimicrobial resistant Enterobacteriaceae urinary isolates from outpatients in Guadeloupe.
Auteur(s) : Guyomard-Rabenirina, Stéphanie Malespine, Joyce Ducat, Célia Sadikalay, Syndia Falord, Mélanie Harrois, Dorothée Richard, Vincent Dozois, Charles
Auteurs secondaires : Institut Pasteur de la Guadeloupe ; Réseau International des Instituts Pasteur (RIIP) - Institut Pasteur de la Guadeloupe Centre Hospitalier de Basse-Terre Institut Pasteur de Nouvelle-Calédonie ; Réseau International des Instituts Pasteur (RIIP) - Institut Pasteur de Nouvelle-Calédonie Institut Armand Frappier (INRS-IAF) ; Institut National de la Recherche Scientifique [Québec] (INRS) - Réseau International des Instituts Pasteur (RIIP) - Institut Armand Frappier Université des Antilles et de la Guyane (UAG) Centre Hospitalo-Universitaire de Pointe-à-Pitre/Abymes This work was financed by grants from the Institut Pasteur International Network, ACIP A13_2011 and by grant FEDER FED-1-1.4 32932 from the European Union.

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Urinary tract infections are bacterial infections most commonly encountered in the community. The resistance rate of uropathogens to commonly prescribed antibiotics has increased worldwide but there are no published data concerning the resistance of strains isolated from community-acquired UTI in Guadeloupe. To assess the susceptibility patterns of Enterobacteriaceae strains isolated from outpatients in Guadeloupe we conducted a prospective study from December 2012 to May 2014 among outpatients consulting at private and public laboratories for urine analysis. Risk factors for E. coli resistance to amoxicillin, third-generation cephalosporin, and ciprofloxacin were also determined. To study the trends of E. coli resistance rates over the past 10 years, data on the susceptibility patterns of E. coli from 2003 to 2014 were also collected from three major laboratories for a retrospective study. During the prospective study, we isolated 1293 bacterial strains from the urine of outpatients presenting for urine analysis. The most commonly isolated bacteria were E. coli (57 %) and Klebsiella pneumoniae (15.5 %). Thirty seven per cent of the E. coli strains were resistant to amoxicillin. Resistance rates to third generation cephalosporin were low for E. coli and other Enterobacteriaceae (3.1 and 12.2 % respectively) and mostly due to the presence of an Extended Spectrum Beta-lactamase. Resistance to cotrimoxazole and ciprofloxacin was moderate (17.8 and 15.6 % respectively). However, the resistance rate of E. coli to ciprofloxacin has significantly increased during the last 10 years. Risk factors were consistent with previously reported data, especially for the increasing ciprofloxacin resistance with age. General practitioners in Guadeloupe need to be better informed to favor the prescription of fosfomycin-trometamol to reduce the risk of resistance to fluoroquinolones.
ISSN: 1471-2180

Droits : http://creativecommons.org/licenses/by/
pasteur-01351191
https://hal-riip.archives-ouvertes.fr/pasteur-01351191
https://hal-riip.archives-ouvertes.fr/pasteur-01351191/document
https://hal-riip.archives-ouvertes.fr/pasteur-01351191/file/art%253A10.1186%252Fs12866-016-0749-9.pdf
PUBMED : 27342199

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Resistance to antimicrobial drugs in different surface waters and wastewaters of Guadeloupe
Auteur(s) : Guyomard-Rabenirina, Stéphanie Dartron, Celia Falord, Mélanie Sadikalay, Syndia Ducat, Célia Richard, Vincent Breurec, Sébastien Gros, Olivier
Auteurs secondaires : Institut Pasteur de la Guadeloupe ; Réseau International des Instituts Pasteur (RIIP) - Institut Pasteur de la Guadeloupe Biologie de la Mangrove (BM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) Institut Pasteur de Nouvelle-Calédonie ; Réseau International des Instituts Pasteur (RIIP) - Institut Pasteur de Nouvelle-Calédonie

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Objective: The first aim of this study was to evaluate the antimicrobial resistance of Enterobacteriaceae in different water environments of Guadeloupe and especially those impacted by waste water treatment plants (WWTP) effluents. The second objective was to characterize the genetic basis for antibiotic resistance of extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase producing Enterobacteriaceae isolates (ESBLE and AmpCE).Methods: We have collected 70 surface waters (river and sea samples) impacted or not by WWTP and 18 waste waters from 2 WWTPs in 2012 and 2013. We i) determined the total and resistant bacterial counts and ii) tested isolated Enterobacteriaceae for their antimicrobial susceptibility. We also studied the genetic basis for antibiotic resistance of ESBLE and AmpCE, and the genetic background of Escherichia coli.Results: In rivers, contamination with Escherichia coli and antibiotic resistant coliforms (ARC) increased from the source to the mouth. Highest levels of river contamination with E. coli (9.26 x 105 MPN/100mL) and ARC (2.26 x 108 CFU/mL) were observed in surface water sampled near the discharge. A total of 246 Enterobacteriaceae strains resistant to antibiotics were isolated, mostly from waste waters and from river water collected near the discharge. Among these strains, 33 were Extended Spectrum Beta Lactamase (ESBLE) and 8 E. coli were AmpC beta-lactamase producers. All the ESBLE were isolated from waste waters or from river water collected near the discharge. The blaCTX-M gene was present in 29 of the 33 ESBLE strains, with 24 belonging to group 1. Numerous strains (68.7%) showed more than one acquired antibiotic resistance mechanism. E. coli strains belonged to different phylogenetic groups; among the B2 group, most strains belonged to the ST131 clone.Conclusion: Our results demonstrated that many human activities can supply antibiotic-resistant bacteria in surface water. Nevertheless, WWTPs were the most important supplier of ESBLE in water environment of Guadeloupe.
ISSN: 1932-6203

Droits : http://creativecommons.org/licenses/by/
hal-01485327
http://hal.upmc.fr/hal-01485327
http://hal.upmc.fr/hal-01485327/document
http://hal.upmc.fr/hal-01485327/file/journal.pone.0173155.pdf
DOI : 10.1371/journal.pone.0173155.t002

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UNDERESTIMATION OF LEPTOSPIROSIS INCIDENCE IN THE FRENCH WEST INDIES ; SOUS-ESTIMATION DE L’INCIDENCE DE LA LEPTOSPIROSE AUX ANTILLES FRANÇAISES
Auteur(s) : Cassadou, Sylvie Rosine, Jacques Flamand, Claude Escher, Martina Ledrans, Martine Bourhy, Pascale Storck-Herrmann, Cécile Guyomard-Rabenirina, Stéphanie
Auteurs secondaires : Agence régionale de santé (ARS) de Martinique (ARS Martinique) Institut Pasteur de la Guyane Centre National de Référence de la Leptospirose-Centre Collaborateur FAO/OMS pour l'épidémiologie de la leptospirose-Biologie des Spirochètes ; Institut Pasteur [Paris] CHU Pointe à Pitre [Guadeloupe] Institut Pasteur de la Guadeloupe CHU de la Martinique Centre Hospitalier de Basse-Terre Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

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