Éditeur(s) : Academic Press Ltd- Elsevier Science Ltd
Résumé : Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence and load in the haemolymph of the shrimps. Changes in all these parameters during the 72-h.p.i. period were recorded for control shrimps and shrimps previously fed for one month with probiotic Pediococcus aciditactici MA18/5M at 10(7) CFU g(-1) of feed. Our results showed that immersion with V nigripulchritudo led to maximal infection level in the haemolymph at 24 h.p.i. preceding the mortality peak recorded at 48 h.p.i. Significant decreases in the antioxidant defences were detected from 24 h.p.i. and beyond that time infection leaded to increases in oxidative stress level and tissue damage.. Compared to control group, shrimps fed the probiotic diet showed lower infection (20% instead of 45% at 24 h.p.i. in the control group) and mortality (25% instead of 41.7% in the control group) levels. Moreover, infected shrimp fed the probiotic compared to uninfected control shrimps exhibited very similar antioxidant status and oxidative stress level. Compared to the infected control group, shrimps fed the probiotic sustained higher antioxidant defences and lower oxidative stress level. This study shows that bacterial infection leads to oxidative stress in L. stylirostris and highlighted a beneficial effect of P. acidilactici, suggesting both a competitive exclusion effect leading to a reduction of the infection level and/or an enhancement of the antioxidant status of the shrimps. (C) 2010 Elsevier Ltd. All rights reserved.
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2010-04 , Vol. 28 , N. 4 , P. 622-631

Droits : 2010 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/00003/11419/7984.pdf
DOI:10.1016/j.fsi.2009.12.024
http://archimer.ifremer.fr/doc/00003/11419/

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’expérience professionnelle à l’étranger peut constituer l’occasion d’une véritable immersion dans le pays et dans la vie quotidienne de ses voisins, collègues, parfois amis, en échappant à la situation « hors-sol » du touriste.Elle semble permettre à l’individu d’occuper une place singulière en n’étant ni étranger ni autochtone, lui donnant accès à des rapports plus égalitaires que le touriste (au moins en impression) avec la population locale ; à la participation à la vie locale ; à une utilité intégrée. Elle offre ainsi la possibilité de réduire la distance avec « l’autre en tant qu’autre », non seulement par le temps passé sur place, mais encore par une plus grande horizontalité avec les habitants.Nous proposons ici d’envisager cette expérience dans sa dimension initiatique, en particulier chez les jeunes pour lesquels c’est une occasion de partir en dehors du cadre touristique, qui peut faire office d’entrée dans l’âge adulte, de plonger dans un univers d’autonomie et d’incertitude. Celui qui part doit affronter un certain nombre d’épreuves, dont il sortira changé.Partant des analyses de J. Campbell sur les trois temps de l’initiation (avant - pendant — après), l’interprétation réclame l’étape du retour en même temps que le retour sur l’expérience. Il s’agit d’une recherche en cours reposant sur une collecte d’entretiens.
Professional experience abroad can be an occasion for being truly immersed in the country, in the life of one’s colleague, sometimes friends, in avoiding the traditional tourist framework.It seems to allow having a peculiar place without the feeling of being a total foreigner neither a local. Allowing a closer relationship with the locals (at least giving that impression). Taking part in the daily life and feeling deeply useful. It offers the possibility to reduce the distance with the “other” as “another” thanks to the time spent on site but also with an improved relationship with the inhabitants.We suggest exploring this experience in its learning aspect, specifically amongst young people. It is an occasion to get out of the usual tourist framework and get fully immersed in autonomy and uncertainty. Upon leaving and trying out this experience one will face challenges which will change him or her forever.Taking the three different analysis from J. Campbell on the three initiating steps (before, during and after the stay) the interpretation requires the return stage as well as the feedback on the experience, providing a collection of interviews.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.11347
http://journals.openedition.org/etudescaribeennes/11347

Éditeur(s) : Inter-Research
Résumé : Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V. penaeicida was developed. It gave an expected amplicon of approximately 310 bp in ethidium bromide-stained agarose gels. The specificity of these primers was assessed with different Vibrio species. Furthermore, DNA extracted by the ChelexTM method could be used to detect fewer than 20 cultured Vibrio cells in seawater or shrimp hemolymph by this assay. It appears to be a reliable screening method for detecting V. penaeicida in shrimp and from the aquatic environment.
Diseases of aquatic organisms (0177-5103) (Inter-Research), 2000-03 , Vol. 49 , N. 2 , P. 109-115

Droits : Inter-Research 2000
http://archimer.ifremer.fr/doc/2000/publication-4436.pdf
DOI:10.3354/dao040109
http://archimer.ifremer.fr/doc/00000/4436/

Éditeur(s) : Inter-Research
Résumé : Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) were purified from diseased freshwater prawns M rosenbergii and used to infect healthy post-larvae (PL) by an immersion method. Three groups of prawns were challenged with various combined doses of MrNV and XSV. Signs of white-tail disease (WTD) were observed in Groups 1 and 2, which had been challenged with combinations containing relatively high proportions of MrNV and low proportions of XSV. By contrast there was little sign of WTD in Group 3, which had been challenged with a higher proportion of XSV than MrNV. A 2-step Taqman real-time RT-PCR was developed and applied to quantify viral copy numbers in each challenged PL. Results showed that genomic copies of both viruses were much higher in Groups 1 and 2 than they were in Group 3, indicating that MrNV plays a key role in WTD of M rosenbergii. The linear correlation between MrNV and XSV genome copies in infected prawns demonstrated that XSV is a satellite virus, dependent on MrNV, but its role in pathogenicity of WTD remains unclear.
Diseases of aquatic organisms (0177-5103) (Inter-Research), 2006-07 , Vol. 71 , N. 1 , P. 11-17

Droits : Inter-Research 2006
http://archimer.ifremer.fr/doc/2006/publication-3568.pdf
DOI:10.3354/dao071011
http://archimer.ifremer.fr/doc/00000/3568/

Éditeur(s) : Mercator Ocean
Résumé : Face to the lack of data to assess precisely the spatial distribution of catches and fishing effort and for the environmental characterization of the fishing area, Ifremer has been implemented since 2005 a new project, Recopesca. It consists in fitting out a sample of voluntary fishing vessels with sensors recording data on fishing effort (and at mid-terms catches) and physical parameters such as temperature or salinity. Recopesca aims at setting up a network of sensors, for scientific purposes, to collect data and improve resources assessment and diagnostics on fisheries, and environmental data required for ecosystem-based management initiatives. The challenge was to develop sensors with no trouble for the fishermen, tough enough to be fixed up on fishing gears, self powered and autonomous. Insofar as the sample of targeted vessels intends to be representative of all the metiers and fleets, the sensors are modular and scalable to collect new data. Different sensors have been implemented: (i) a temperature-salinity sensor, able to record physical parameters, depth and duration of immersion, for passive and active gears, and (ii) a specific sensor to record number or length of passive gears. A GPS monitors the position of the vessels and the temperature or salinity profiles and series. Each sensor is equipped with a radio device transferring the data to a receiver on-board, called “concentrator” that sends the data to Ifremer central databases by GPRS. An anti-rolling weigh-scale has been developed and is currently on test to record catches per species and fishing operation. The presentation will show the first data and results of this participative approach.
Mercator Ocean - Quarterly Newsletter (Mercator Ocean), 2010-04 , N. 37 , P. 40-48

Droits : 2010 Mercator Ocean
http://archimer.ifremer.fr/doc/00024/13500/10514.pdf
http://archimer.ifremer.fr/doc/00024/13500/