Éditeur(s) : HAL CCSD
Résumé : Background. Chlorine is the most widely used disinfectant for drinking water treatment. During treatment process, the natural organic matter present in water reacts with chlorine to form disinfection by-products (DBPs), such as trihalomethanes (THMs) and haloacetic acids (HAAs), the most occurring ones. Several identified DBPs were recognized carcinogens or fetotoxic in animals. We conducted two epidemiological studies to assess the health effects of human exposure to DBPs regarding cancer and reproduction. Association between THM exposure and the risk of bladder cancer: a pooled analysis of 3 European case-control studies. The study included 2381 cases and 3086 controls from 3 case-control studies (France, Finland, Spain). Environmental exposure to SPCs was measured by THM concentrations in water distribution systems, retrospectively estimated through the 40-year exposure window. Information about water uses (tap water ingestion, showers and baths, swimming-pool attendance) was available. An increased risk of bladder cancer was observed, in men only, with increasing THM concentrations in water systems and duration of exposure to chlorinated surface water. No association was observed with exposure through tap water ingestion. Exposure through showers, baths and swimming in pools (available in Spanish study only) was associated with an increased risk of bladder cancer. Three North-American case-control studies were joined to the three European ones into a meta-regression. No specific dose-response relationship was identified for European studies. A global dose-response relationship was consequently estimated, including 4351 cases and 7055 controls. Association between DBP exposure and the risk of preterm birth and Intra-Uterine Growth Retardation (IUGR). This study was conducted within the PELAGIE cohort (3400 pregnant women included in early pregnancy, in Brittany, France, between 2002 and 2006). Exposure during pregnancy was measured by 2 indicators: THM concentrations in water distribution systems and a biomarker of HAA ingestion, the level of trichloroacetic acid in maternal urine at inclusion (in a nested case-control study). The regulatory database SISE-Eaux was used to estimate THMs concentrations in the water distribution systems. Water uses during pregnancy (ingested tap water, swimming-pool attendance, showers and bath frequencies and duration) were collected with questionnaires. Our results suggest an association between prenatal exposure to DBPs (THMs and HAAs) and the risk of IUGR. No association was found with the risk of preterm birth.
Contexte. La chloration est le traitement le plus utilisé pour désinfecter l’eau distribuée à la population. Lors du traitement, des sous-produits de chloration (SPCs) se forment par réaction de la matière organique naturelle présente dans l’eau avec le chlore. Les produits les plus présents sont les trihalométhanes (THMs), les acides haloacétiques (HAAs). Des études toxicologiques (animales et in vitro) ont montré la génotoxicité et/ou carcinogénicité et la reprotoxicité de certains SPCs. Nous avons mené deux études évaluant les effets sur la santé humaine de l’exposition aux SPCs, dans le domaine du cancer et de la reproduction. Association entre exposition aux THMs et risque de cancer de la vessie : une analyse poolée de 3 études cas-témoins européennes. Cette analyse a inclus 2381 cas et 3086 témoins issus de 3 études cas-témoins (France, Espagne, Finlande). L’exposition environnementale aux SPCs a été mesurée par la concentration en THMs estimée rétrospectivement dans les réseaux de distribution d’eau au cours des 40 années de la fenêtre d’exposition. Les usages de l’eau connus sont l’ingestion, les douches et les bains, la fréquentation de piscine (étude espagnole seulement). Une relation croissante a été observée entre le niveau environnemental de THMs, la durée d’exposition à une eau de surface chlorée et le risque de cancer de la vessie, chez les hommes uniquement. Aucune association n’a été observée avec l’exposition via l’ingestion d’eau du robinet. L’exposition via les douches, les bains et la piscine est apparue liée au risque de cancer de la vessie. Trois études cas-témoins nord-américaines ont été intégrées dans une méta-régression. Aucune spécificité européenne de la relation dose-réponse n’a été mise en évidence. Une relation dose-réponse globale incluant 4351 cas et 7055 témoins a été estimée. Association entre exposition aux SPCs et risque de prématurité et de retard de croissance intra-utérin (RCIU). Cette étude est issue de la cohorte bretonne Pélagie (3400 femmes enceintes recrutées en début de grossesse entre 2002 et 2006). L’exposition pendant la grossesse a été mesurée à l’aide de 2 indicateurs : la concentration en THMs de l’eau distribuée dans les réseaux et le dosage d’un biomarqueur urinaire (acide trichloroacétique) pour un sous-échantillon de femmes (étude cas-témoins nichée). Les niveaux dans les réseaux proviennent de la base de données réglementaire SISE-Eaux. Les usages de l’eau du robinet par les femmes pendant la grossesse ont été collectés par questionnaire (quantité d’eau du robinet bue, fréquentation de la piscine, fréquence et durée des douches et des bains). Notre étude suggère une association entre l’exposition prénatale aux SPCs et le risque de RCIU. Aucune association n’est observée avec le risque de prématurité.
https://tel.archives-ouvertes.fr/tel-00927735

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2013REN1B008
tel-00927735
https://tel.archives-ouvertes.fr/tel-00927735
https://tel.archives-ouvertes.fr/tel-00927735/document
https://tel.archives-ouvertes.fr/tel-00927735/file/COSTET_DEIBER_Nathalie4.pdf

Éditeur(s) : HAL CCSD WB Saunders
Résumé : International audience
BACKGROUND: Environmental surveillance is important in high-risk areas of hospitals to prevent fungal infections in immunosuppressed patients. Conventional culture methods for enumerating environmental fungi are time-consuming. AIM: In this field study, a solid-phase cytometry technique (SPC) and a more conventional culture-based method to quantify fungal contamination of hospital air and surface samples were compared. METHODS: For the air sampling, a liquid cyclone air sampler was used with a flow rate of 300 L/min for 10 min in each of four hospital locations. Surface swabbing was done in two locations, with two different swab types. Samples from all areas were processed by SPC and by culture on malt extract agar. FINDINGS: The mean airborne concentrations of viable fungi determined by SPC were about 1.5-fold higher than the mean concentrations obtained with the culture-based method. These differences for air samples were significant in three hospital environments. No significant difference was observed for surface samples between the two swab types and between the two analytical methods. One of the prominent advantages of SPC was its rapidity in comparison with the culture-based method (5 h versus 5 days). CONCLUSION: In this study, we showed that SPC allows for rapid monitoring of viable fungi in hospital environments. SPC can thus be used to provide an early warning and a rapid implementation of corrective measures. Viable fungi detection may be an important tool to assess infectious risk in wards with immunosuppressed patients.
ISSN: 0195-6701

hal-00874713
https://hal.archives-ouvertes.fr/hal-00874713
DOI : 10.1016/j.jhin.2012.10.004
PUBMED : 23313088

Éditeur(s) : Elsevier Science Bv
Résumé : There has been growing interest in the development of mud crab aquaculture in New Caledonia. However, for this to become established at a commercial level, a cost-effective formulated feed based on internationally-available ingredients needs to be developed. We have evaluated the optimal dietary protein content for juvenile crabs, Scylla serrata (Forskål, 1775), using a series of diets with a protein content ranging from 27 to 49% and soy protein concentrate (SPC) as the main protein source. For this purpose, 54 individually housed crabs were allocated to five dietary treatments (n = 10 or 11). The crabs were fed ad libitum, for 81 days with the allocated diets. The apparent digestibilities of dry mater, crude protein and energy were high (96.2-97.3%), irrespective of the diet. The voluntary feed intake (VFI) of crabs widely varied from 46 to 220 g kg- 1of fresh initial body weight per week (iBW week- 1) whatever the diet. However, SPC intake and protein intake increased significantly with dietary protein content up to the diet with 40% crude protein, but did not increase further with diets containing 44% and 49% crude protein. The cumulative molts were strongly affected by the VFI levels or energy intake and also, to a lesser extent, by the levels of SPC or protein in diets. Two phases in tissue gain were observed after ecdysis: an initial deposition phase lasting around 30 days followed by a plateau which lasted until the next molt. The daily tissue growth was 16.5% of dry body weight (dry BW) one day after ecdysis and dramatically decreased to 3.6% of dry BW over the first 10 days, then decreased more slowly to the minimum value of 1.3% of dry BW over the next 70 days. During the course of experiment, the best growth (tissue growth and molt frequency) and the best feed efficiency (FCR, PER, retention of proteins and lipids) were obtained with crabs fed on the diet with 40% crude protein. This result was confirmed by a bioenergetic study which showed significantly higher allocation of the energy intake for growth (RE) of crabs fed on diet 40% crude protein. Finally, under our experimental conditions, 1 kg of juvenile crabs required 6.5 ± 1.1 g of protein per day. This level was obtained with the diet SPC-42 that contained 40% of protein of which almost three quarters were derived from SPC. Two hypotheses are proposed to explain the negative effect of high level of SPC or protein on growth and feed efficiency for crabs fed on in diets containing 52% and 60% SPC.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-10 , Vol. 434 , P. 499-509

Droits : 2014 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00211/32196/30630.pdf
http://archimer.ifremer.fr/doc/00211/32196/

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : Over the last 16 years, the Secretariat of the Pacific Community (SPC - formerly the South Pacific Commission) has been developing and providing information on mid-water fishing techniques targeting the larger, deeper-swimming tunas that aggregate around FADs. SPC masterfishermen have conducted in-country fishing trials to test methods, such as vertical longlines, and train local fishermen in their construction and use. Vertical longline catch rates have varied considerably due to a range of reasons with no catches reported in some locations, or just sharks, to highs of over 6.5 kg /10 hooks/hour being recorded in other locations. Other methods like drop-stone, palu-ahi, and single-hook drifting lines have been developed within the region, with SPC adopting and transferring this technology to other locations. To further disseminate this information, SPC has recently published a technical manual outlining a range of mid-water fishing gears and techniques that can be used in association with FADs. An outline of the main gears and techniques with catch data where available is presented, with factors influencing catches discussed based on the findings of SPC masterfishermen over the years.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15295/12653.pdf
http://archimer.ifremer.fr/doc/00042/15295/

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : Since the early 1980's, the Secretariat of the Pacific Community (SPC, formerly the South Pacific Commission) has contributed to the development of FAD programmes for the artisanal fisheries sector in the Pacific region. In particular, it has put considerable efforts into developing technology that would assure a two-year average FAD lifespan, while maintaining costs under US$ 4,000 per FAD unit. From 1996 to 1998, this work led to the publication of three volumes of the SPC FAD Manual. Volume 2 of this manual, "Rigging Deep-Water FAD Moorings", describes in great detail two FAD models that were developed from the original 1983 SPC-recommended model, and gives precise instructions on the way to construct them. Despite SPC's work, the objectives set, in particular the two-year average lifespan for FADs, have not been consistently met, resulting in many FAD programmes in the region being suspended. An analysis of the reasons why this has occurred is presented, along with a short description of the practical research that could be pursued, using different objectives, to introduce new FAD programmes in the region.Original Abstract: Depuis le debut des annees quatre-vingt, le secretariat general de la communaute du Pacifique (SPC, anciennement commission du Pacifique Sud) a soutenu et accompagne le developpement de programmes DCP destines au secteur de la peche artisanale, dans la region Pacifique. Il s'est en particulier efforce de mettre au point des modeles de DCP repondant principalement aux deux criteres suivants: duree de vie superieure a deux ans et cout unitaire moyen inferieur a 4 000 dollars US. Entre 1996 et 1998, ces travaux ont abouti a la publication en trois volumes du manuel de la SPC sur les DCP. Le volume 2 de ce manuel, " La fabrication de DCP pour grandes profondeurs ", presente deux evolutions d'un modele concu en 1983 et en detaille tres precisement les methodes de fabrication. Le present document analyse les raisons pour lesquelles, malgre les efforts deployes, les objectifs fixes, et en particulier la duree de vie minimum de deux ans pour les DCP, n'ont pas ete pleinement atteints et la plupart des programmes DCP de la region ont ete interrompus. Il donne egalement une breve description des axes de recherche appliquee qui pourraient etre suivis, en se fixant d'autres objectifs, pour relancer des programmes DCP dans la region.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15291/12648.pdf
http://archimer.ifremer.fr/doc/00042/15291/

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
Tight control of basal cytosolic Ca(2+) concentration is essential for cell survival and to fine-tune Ca(2+)-dependent cell functions. A way to control this basal cytosolic Ca(2+) concentration is to regulate membrane Ca(2+) channels including store-operated Ca(2+) channels and secondary messenger-operated channels linked to G-protein-coupled or tyrosine kinase receptor activation. Orai, with or without its reticular STIM partner and Transient Receptor Potential (TRP) proteins, were considered to be the main Ca(2+) channels involved. It is well accepted that, in response to cell stimulation, opening of these Ca(2+) channels contributes to Ca(2+) entry and the transient increase in cytosolic Ca(2+) concentration involved in intracellular signaling. However, in various experimental conditions, Ca(2+) entry and/or Ca(2+) currents can be recorded at rest, without application of any experimental stimulation. This led to the proposition that some plasma membrane Ca(2+) channels are already open/activated in basal condition, contributing therefore to constitutive Ca(2+) entry. This article focuses on direct and indirect observations supporting constitutive activity of channels belonging to the Orai and TRP families and on the mechanisms underlying their basal/constitutive activities.
ISSN: 0175-7571

hal-01528591
https://hal.archives-ouvertes.fr/hal-01528591
DOI : 10.1007/s00249-017-1216-8
PUBMED : 28516266

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999 15-19 octobre 1999
Résumé : In the area served by the Secretariat of the Pacific Community (SPC), which includes 22 Pacific Island countries and territories, anchored FADs have been used since the late 1970s. First introduced from the Philippines, via Hawaii, they were quickly adopted by both industrial and artisanal fisheries sectors : in 1984 more than 600 anchored FADs had been deployed in the region. Since these early days, the development of the technique by the industrial and the artisanal sectors have followed parallel paths with little interaction. In the industrial private sector, companies are funding, deploying and monitoring their own FADs. For some fishing companies, using purse seiners and pole-and-line vessels, from the Solomon Islands or Papua-New-Guinea, these FADs have become a necessity. FAD programmes for small-scale fisheries have been almost exclusively run by the public sector, with technical support from regional and international development agencies and financial assistance from overseas funding agencies. These programmes have had mixed successes : becoming an on-going and essential tool in some places like French Polynesia or Guam; or being momentarily suspended, like in Vanuatu or Tonga, because of the lack of funds, partly due to the scarcity of proven economic return to the fishing communities. This document is an attempt to synthesise the current information on these very diverse situations, including technical, economical and social considerations.

Droits : Ifremer
http://archimer.ifremer.fr/doc/00042/15283/12669.pdf
http://archimer.ifremer.fr/doc/00042/15283/