Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD
Résumé : International audience
Up-to-date estimates of the health outcomes of preterm children are needed for assessing perinatal care, informing parents, making decisions about care, and providing evidence for clinical guidelines. To determine survival and neonatal morbidity of infants born from 22 through 34 completed weeks' gestation in France in 2011 and compare these outcomes with a comparable cohort in 1997. The EPIPAGE-2 study is a national, prospective, population-based cohort study conducted in all maternity and neonatal units in France in 2011. A total of 2205 births (stillbirths and live births) and terminations of pregnancy at 22 through 26 weeks' gestation, 3257 at 27 through 31 weeks, and 1234 at 32 through 34 weeks were studied. Cohort data were collected from January 1 through December 31, 1997, and from March 28 through December 31, 2011. Analyses for 1997 were run for the entire year and then separately for April to December; the rates for survival and morbidities did not differ. Data are therefore presented for the whole year in 1997 and the 8-month and 6-month periods in 2011. Survival to discharge and survival without any of the following adverse outcomes: grade III or IV intraventricular hemorrhage, cystic periventricular leukomalacia, severe bronchopulmonary dysplasia, retinopathy of prematurity (stage 3 or higher), or necrotizing enterocolitis (stages 2-3). A total of 0.7% of infants born before 24 weeks' gestation survived to discharge: 31.2% of those born at 24 weeks, 59.1% at 25 weeks, and 75.3% at 26 weeks. Survival rates were 93.6% at 27 through 31 weeks and 98.9% at 32 through 34 weeks. Infants discharged home without severe neonatal morbidity represented 0% at 23 weeks, 11.6% at 24 weeks, 30.0% at 25 weeks, 47.5% at 26 weeks, 81.3% at 27 through 31 weeks, and 96.8% at 32 through 34 weeks. Compared with 1997, the proportion of infants surviving without severe morbidity in 2011 increased by 14.4% (P < .001) at 25 through 29 weeks and 6% (P < .001) at 30 through 31 weeks but did not change appreciably for those born at less than 25 weeks. The rates of antenatal corticosteroid use, induced preterm deliveries, cesarean deliveries, and surfactant use increased significantly in all gestational-age groups, except at 22 through 23 weeks. The substantial improvement in survival in France for newborns born at 25 through 31 weeks' gestation was accompanied by an important reduction in severe morbidity, but survival remained rare before 25 weeks. Although improvement in survival at extremely low gestational age may be possible, its effect on long-term outcomes requires further studies. The long-term results of the EPIPAGE-2 study will be informative in this regard.
ISSN: 2168-6211

hal-01258705
http://hal.univ-brest.fr/hal-01258705
PUBMED : 25621457
DOI : 10.1001/jamapediatrics.2014.3351

Éditeur(s) : HAL CCSD
Résumé : The Karakorum Fault zone (KFZ), lying in the west of the Tibetan plateau, is an important dextral strike-slip fault and the important northern geological boundary of the west Himalaya. In the AyilaRi' gyu Range-Gai Basin area lying in the southeast of the KFZ, Ar-40/Ar-39 thermochronologic analysis was performed on the syn-tectonic minerals in the, metamorphic rocks in the Karakorum ductile shear zone, with the base of petrology and deformation structure researches. According to the microstructure features, the minerals in the shear zone recorded the continuous deformation from high ( > 600 degrees C) to low temperature ( < 250 degrees C) appeared as that the nearly horizontal dextral shearing has transformed into the oblique dextral normal slip, made the greenschist facies of deformation stack oil the early medium-high temperature deformation, indicated the uplift during the slip. The thermochronologic revealed the continuous shear process from the early Miocene to 4Ma at least, which containing three rapid cooling stage: from 25 similar to 22Ma to 21 similar to 18Ma, the deformation locally stopped or slower in the, shallow high temperature shear process; from 15Ma to 12Ma, the rapid uplift of the AyilaRi' gyu Range, the initial formation of the Gar Basin together with the main deep incision of river during the strip-slip of the KFZ; since 9Ma, the AyilaRi' gyu Range has increased the rapid uplift and the Gar Basin has formed finally. According to the different levels of deformation and the dextral displacement of the surface landform with different ages, the long term slip rate and the uplift rate indicated the intense dextral strike slip and uplift associated since the late Quarternary Period.
Acta Petrologica Sinica

hal-00411572
https://hal.archives-ouvertes.fr/hal-00411572

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Fractures or discontinuities perpendicular to the least stress σ3 were generated in a synthetic rock analogue (granular, frictional, cohesive, and dilatant) material in axisymmetric extension tests. These fractures are of two types defined by the mean stress σ. When σ is very small, the fractures form through the mode I cracking at tensile σ3 equal to the material tensile strength. The fracture walls have smooth surfaces. At higher σ, these surfaces become rougher, with the topography features forming faint or delicate plumose patterns. The amplitude of the plumose topography increases with σ. The axial stress σ3 at fracturing reduces in magnitude and changes in sign with the σ increase. Thus σ3 orthogonal discontinuities can form at compressive σ3. SEM observations show that these discontinuities are deformation localization bands where the material is characterized by the heterogeneous decohesion and volume and porosity increase due to dilatancy. The band thickness is several grain sizes. At formation, the bands are not opened, so they are not mode I fractures. They become fractures with plumose fractography after the separation of the sample parts along the band. The formation mechanism of these discontinuities or fractures is not completely clear, but it is suggested that it represents a running constitutive instability in the form of dilatancy banding (with further σ increase the bands become inclined to σ1, i.e., shear). The similarity between the experimentally generated plumose surface fractures and natural joints is discussed, and it is suggested that they can be formed as propagating constitutive instabilities.
ISSN: 0148-0227

hal-00585846
https://hal.archives-ouvertes.fr/hal-00585846
DOI : 10.1029/2010JB008104