| A microfluidic device to study red blood cell deformability Auteur(s) : El Nemer, Wassim Ndour, Papa Alioune Lefevre, Sophie D. Tawfik, Harvey Picot, Julien Le Pioufle, Bruno Buffet, Pierre Le Van Kim, Caroline Auteurs secondaires : SATIE-BIOMIS ; Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE) ; CNRS - École normale supérieure - Cachan (ENS Cachan) - CNRS - École normale supérieure - Cachan (ENS Cachan) Éditeur(s) : HAL CCSD Résumé : International audience In this paper we present a microfluidic architecture dedicated to the study of Red Blood Cells (RBCs) deformability in microchannel smaller than the cell shape (biconcave disc of 7-8 μm in diameter) and cell capability to pass through slits separated by a gap value down to 2 μm, acting as a filter. This device is use in the context of RBCs disorders affecting their deformability, which is analysed versus number of cells trapped in the filter. This device can be seen as mimicking the human spleen. Microfabrication of the chip is based on polydimethylsiloxane (PDMS) associated to a two level thick photoresist mould (SU8). One level is used for microfluidic access with a 25 μm thickness towards the studied zone. The second level concerns the mimicking zones where RBCs flow through 5 μm down to 2 μm-wide slits acting like a mechanical filter. This level has a thickness of 5 μm. Devices have been tested on RBCs having specific disorder on their mechanical elasticity in order to validate the design principle. Two cases are presented in this paper: a.) Sickle RBCs inducing cell rigidity at two levels b.) Parasitized RBCs by Plasmodium falciparum. On both cases, we clearly show that deformability of RBCs influences the capability to pass through small slits (down to 2um) and thus is in relation with the pathology stage. This spleen-like microfluidic chip mimicking the RBCs filtering function is a promising tool for the exploration of RBC disorders combined with mechanical alterations. 4th European Conference on Microfluidics Limerick, Ireland hal-01113061 https://hal.archives-ouvertes.fr/hal-01113061 | Partager
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