Manioc : Portail | resultats 1 - 1 pour la recherche: This work was supported by a European commission REGPOT-CT-2011-285837-STRonGer Grant within the FP7, by an Institut Pasteur ACIP (A10-2013) Grant and by Investissement d’Avenir Grant of the ANR (CEBA: ANR-10-LABX-25-01).

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Use of Plasmodium falciparum culture-adapted field isolates for in vitro exflagellation-blocking assay.
Auteur(s) : Leba, Louis-Jérôme Musset, Lise Pelleau, Stéphane Estevez, Yannick Birer, Caroline Briolant, Sébastien Witkowski, Benoit Ménard, Didier
Auteurs secondaires : Laboratoire de Parasitologie ; Institut Pasteur de la Guyane - Réseau International de Instituts Pasteur Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS) Direction Interarmées du Service de Santé en Guyane ; Direction Interarmées du Service de Santé en Guyane Institut de Recherche Biomédicale des Armées (IRBA) ; Service de Santé des Armées Institut Pasteur du Cambodge ; Institut Pasteur du Cambodge - Réseau International des Instituts Pasteur (RIIP) Department of Life Sciences ; Imperial College London This work was supported by a European commission REGPOT-CT-2011-285837-STRonGer Grant within the FP7, by an Institut Pasteur ACIP (A10-2013) Grant and by Investissement d’Avenir Grant of the ANR (CEBA: ANR-10-LABX-25-01). ANR-10- LABX-25-01, CEBA, Labex CEBA ANR-10- LABX-25-01

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
A major requirement for malaria elimination is the development of transmission-blocking interventions. In vitro transmission-blocking bioassays currently mostly rely on the use of very few Plasmodium falciparum reference laboratory strains isolated decades ago. To fill a piece of the gap between laboratory experimental models and natural systems, the purpose of this work was to determine if culture-adapted field isolates of P. falciparum are suitable for in vitro transmission-blocking bioassays targeting functional maturity of male gametocytes: exflagellation. Plasmodium falciparum isolates were adapted to in vitro culture before being used for in vitro gametocyte production. Maturation was assessed by microscopic observation of gametocyte morphology over time of culture and the functional viability of male gametocytes was assessed by microscopic counting of exflagellating gametocytes. Suitability for in vitro exflagellation-blocking bioassays was determined using dihydroartemisinin and methylene blue. In vitro gametocyte production was achieved using two isolates from French Guiana and two isolates from Cambodia. Functional maturity of male gametocytes was assessed by exflagellation observations and all four isolates could be used in exflagellation-blocking bioassays with adequate response to methylene blue and dihydroartemisinin. This work shows that in vitro culture-adapted P. falciparum field isolates of different genetic background, from South America and Southeast Asia, can successfully be used for bioassays targeting the male gametocyte to gamete transition, exflagellation.
ISSN: 1475-2875

Droits : http://creativecommons.org/licenses/by/
pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/file/LeBas%20et%202015%20version%20publie%20%281%29.pdf
DOI : 10.1186/s12936-015-0752-x

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