Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Springer-Verlag
Résumé : We provide evidence for a plume-like upwelling beneath the Eifel hotspot, Western Germany, by using teleseismic shear-wave splitting to resolve the anisotropy associated with upwelling flow that is spreading laterally into the asthenosphere. The variation in fast-polarization azimuth we find across the Eifel hotspot is explained by a model of slowly upwelling material that is horizontally being deflected or sheared in a parabolic asthenospheric flow (PAF) pattern toward west-southwest, a direction that correlates with Eurasian absolute plate motion. We suggest that the lack of an age progression for Eifel volcanism, which is expected for a fixed-upwelling model, is a result of (1) sporadic volcanism due to a low excess plume temperature and/or varying crustal stresses that periodically relax and facilitate eruption, and (2) complex upwelling flow pathways and/or Late Tertiary changes in the slow Eurasian plate motion. The success of the PAF model in fitting the data is remarkable given the small number of parameters (four) and the consistency with the plate motion direction determined from geology and/or geodesy. This suggests that a predictable mantle-anisotropy pattern may exist also for other hotspots driven by plume-like upwellings, and that splitting can be a useful diagnostic to differentiate between plume-like and alternative sources for mantle hotspots.
Mantle plumes - a multidisciplinary approach

hal-00407986
https://hal.archives-ouvertes.fr/hal-00407986
DOI : 10.1007/978-3-540-68046-8