Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
The epigenetic events imposed during germline reprogramming and affected by harmful exposure can be inherited and transferred to subsequent generations via gametes inheritance. In this study, we examine the transgenerational effects promoted by widely used herbicide atrazine (ATZ). We exposed pregnant outbred CD1 female mice and the male progeny was crossed for three generations with untreated females. We demonstrate here that exposure to ATZ affects meiosis, spermiogenesis and reduces the spermatozoa number in the third generation (F3) male mice. We suggest that changes in testis cell types originate from modified transcriptional network in undifferentiated spermatogonia. Importantly, exposure to ATZ dramatically increases the number of transcripts with novel transcription initiation sites, spliced variants and alternative polyadenylation sites. We found the global decrease in H3K4me3 occupancy in the third generation males. The regions with altered H3K4me3 occupancy in F3 ATZ-derived males correspond to altered H3K4me3 occupancy of F1 generation and 74% of changed peaks in F3 generation are associated with enhancers. The regions with altered H3K4me3 occupancy are enriched in SP family and WT1 transcription factor binding sites. Our data suggest that the embryonic exposure to ATZ affects the development and the changes induced by ATZ are transferred up to three generations.
ISSN: 0305-1048

hal-01477213
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477213
DOI : 10.1093/nar/gkw840
PUBMED : 27655631

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The capacity of testicular somatic cells to promote and sustain germ cell differentiation is largely regulated by sexual steroids and notably androgens. In fish species the importance of androgens is emphasized by their ability to induce sex reversal of the developing fries and to trigger spermatogenesis. Here we studied the influence of androgens on testicular gene expression in trout testis using microarrays. Following treatment of immature males with physiological doses of testosterone or 11-ketotestosterone, 418 genes that exhibit changes in expression were identified. Interestingly, the activity of testosterone appeared stronger than that of 11-ketotestosterone. Expression profiles of responsive genes throughout testis development and in isolated germ cells confirmed androgens to mainly affect gene expression in somatic cells. Furthermore, specific clusters of genes that exhibit regulation coincidently with changes in the natural circulating levels of androgens during the reproductive cycle were highlighted, reinforcing the physiological significance of these data. Among somatic genes, a phylogenetic footprinting study identified putative androgen response elements within the proximal promoter regions of 42 potential direct androgen target genes. Finally, androgens were also found to alter the germ line towards meiotic expression profiles, supporting the hypothesis of a role for the somatic responsive genes in driving germ cell fate. This study significantly increases our understanding of molecular pathways regulated by androgens in vertebrates. The highly cyclic testicular development in trout together with functions associated with regulated genes reveal potential mechanisms for androgen actions in tubule formation, steroid production, germ cell development and sperm secretion.
ISSN: 1932-6203

inserm-00825763
http://www.hal.inserm.fr/inserm-00825763
http://www.hal.inserm.fr/inserm-00825763/document
http://www.hal.inserm.fr/inserm-00825763/file/Rolland_et_al.pdf
DOI : 10.1371/journal.pone.0053302

Éditeur(s) : HAL CCSD American Association for Cancer Research
Résumé : International audience
Triple-negative breast cancers (TNBC) lacking estrogen and progesterone receptors and HER2 amplification have a relatively high risk of metastatic dissemination, but the mechanistic basis for this risk is not understood. Here we report that serum levels of CD95L are higher in TNBC patients compared to other breast cancer patients. Metalloprotease-mediated cleavage of CD95L expressed by endothelial cells surrounding tumors generates a gradient that promotes cell motility, due to formation of an unconventional CD95-containing receptosome termed the motility-inducing signaling complex. Formation of this complex was instrumental for Nox3-driven ROS generation. Mechanistic investigations revealed a Yes-Orai1-EGFR-PI3K pathway that triggered migration of TNBC cells exposed to CD95L. Our findings establish a pro-metastatic function for metalloprotease-cleaved CD95L in triple-negative breast cancers, revisiting its role in carcinogenesis.
ISSN: 0008-5472

hal-00873634
https://hal.archives-ouvertes.fr/hal-00873634
PUBMED : 24072745
DOI : 10.1158/0008-5472.CAN-13-1794

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’article propose une réflexion sur le positionnement touristique de la Martinique confrontée à une rude concurrence régionale. L’analyse porte sur les potentialités de cette petite île montagneuse (1128 km2) en matière d’écotourisme forestier. Cette nouvelle orientation touristique doit permettre d’enrichir une offre touristique qui se limite encore aux simples pratiques balnéaires. L’écotourisme peut aussi être un outil de gestion des ressources forestière et plus généralement il est susceptible de soutenir des activités dans les secteurs montagneux les moins développés de la Martinique.
The article is an evaluation of the tourist situation in Martinique now facing stiff regional competition. The analysis focuses on the potential of this small mountainous island (1128 km2) in the area of forest ecotourism. This new focus is meant to enhance a tourist potential which is still largely limited to the seaside and its attractions. Ecotourism can also be a tool not only for forest resource management but also a means for promoting development initiatives in the less developed mountainous regions of Martinique.
Martinique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.440
http://journals.openedition.org/etudescaribeennes/440

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’écotourisme ouvre un cadre conceptuel original qui permet d’envisager des démarches renouvelées de développement à partir des revenus issus de consommations touristiques mieux réfléchies et organisées, susceptibles de soutenir des initiatives de développement local s’inscrivant dans un projet global de développement durable. Variante douce du tourisme, loin du tourisme de masse, le champ de l’écotourisme s’étend aux confins du tourisme de nature, du tourisme culturel et des pratiques scientifiques et sportives de plein air. Une définition de l’écotourisme peut être proposée à partir des six points suivants : 1/ nature et culture, 2/ le bien-être des sociétés hôtes, 3/ des touristes responsables, 4/ la participation des sociétés hôtes, 5/ la durabilité, 6/ l’art de la rencontre.
Ecotourism provides an original conceptual framework that enables approaches to development that are better able to provide a more equitable distribution of income, as well as better organized and thought through initiatives which are more likely to promote a holistic sustainable local development. With somewhat less negative impact than normal tourism, considerably less that mass tourism, ecotourism can encompass nature tourism, cultural tourism, scientific exploration, and even outdoor sports... A definition of ecotourism should take into account the following six points: 1/ nature and culture, 2/ the welfare of the host community, tourists acting responsibly, 3/ participation by the local community, 5 / sustainability , 6/ promotion of cultural exchanges

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.414
http://journals.openedition.org/etudescaribeennes/414

Éditeur(s) : Elsevier
Résumé : Changes in biodiversity may disrupt the ecological functions performed by species assemblages. Hence, we urgently need to examine the implications of biodiversity loss not only in terms of species conservation but also in terms of sustainability of ecosystem services. The ability of protected areas to maintain local species richness has been clearly demonstrated. However, preserving goods and services provided by ecosystems requires not only the conservation of species richness but also the conservation of the most 'original' species, i.e. the ones with the highest average rarity of their attributes which are likely to perform some unique functions in ecosystems. We proposed a new conservation of biological originality (CBO) index as well as associated randomization tests to quantify the ability of piotected areas to maintain viable populations for the most original species. As an application, we used long-term fisheries data collected in the Bonifacio Strait Natural Reserve (BSNR) to determine the species which benefited from the protection reinforcement in 1999. We also estimated a set of 14 ecomorphological functional traits on the 37 fish species caught in the BSNR and we obtained a functional originality value for each species. As a result, we found that functional originality was significantly protected in the fish assemblage of the BSNR: species with the most original functional trait combinations became more abundant after 1999. Our finding suggests that protecting most original species is an insurance against functional diversity erosion in the BSNR. More generally, our new index can be used to test whether protected areas may protect preferentially the most original species and whether restorative management promotes the reestablishment of the most original species with particular habitat requirements. (c) 2008 Elsevier Ltd. All rights reserved.
Biological Conservation (0006-3207) (Elsevier), 2008-06 , Vol. 141 , N. 6 , P. 1569-1580

Droits : 2008 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/2008/publication-4480.pdf
DOI:10.1016/j.biocon.2008.04.002
http://archimer.ifremer.fr/doc/00000/4480/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
CD95 ligand (CD95L) is expressed by immune cells and triggers apoptotic death. Metalloprotease-cleaved CD95L (cl-CD95L) is released into the bloodstream but does not trigger apoptotic signaling. Hence, the pathophysiological role of cl-CD95L remains unclear. We observed that skin-derived endothelial cells from systemic lupus erythematosus (SLE) patients expressed CD95L and that after cleavage, cl-CD95L promoted T helper 17 (Th17) lymphocyte transmigration across the endothelial barrier at the expense of T regulatory cells. T cell migration relied on a direct interaction between the CD95 domain called calcium-inducing domain (CID) and the Src homology 3 domain of phospholipase C gamma 1. Th17 cells stimulated with cl-CD95L produced sphingosine-1-phosphate (S1P), which promoted endothelial transmigration by activating the S1P receptor 3. We generated a cell-penetrating CID peptide that prevented Th17 cell transmigration and alleviated clinical symptoms in lupus mice. Therefore, neutralizing the CD95 non-apoptotic signaling pathway could be an attractive therapeutic approach for SLE treatment.
ISSN: 1074-7613

hal-01359568
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359568
DOI : 10.1016/j.immuni.2016.06.028
PUBMED : 27438772

Éditeur(s) : HAL CCSD Université de Nantes
Résumé : International audience
La promotion internationale des démarches d'investigation (DI) et l'enrôlement sous cette bannière commune, en France, d'approches prônées en sciences expérimentales, en technologie et en mathématiques interrogent sur le(s) sens de ce terme, sur son unité ou sa diversité. Toute mise en activité d'élèves paraît parfois suffire à parler de DI, dès lors que ceux-ci sont actifs ou acteurs. Engager des apprenants dans des DI a cependant un sens profond, qu'éclairent ses origines : des considérations épistémologiques et psychologiques ont historiquement concouru à préconiser une approche dans laquelle les composantes essentielles de l'esprit de recherche, que sont l'esprit créatif et l'esprit de contrôle, peuvent trouver leur place dans le vécu des classes. Des tentatives de définition et de caractérisation ont été faites, sans toujours mettre en relief ces principes fondamentaux. Remettre ceux-ci au premier plan permet d'envisager la spécification de critères pouvant tenir lieu, dans une proposition à visée normative – sans prétendre fixer de norme ultime – de préceptes utiles pour définir et élaborer des DI.
ISSN: 1954-3077

Droits : info:eu-repo/semantics/OpenAccess
hal-01535192
https://hal.univ-antilles.fr/hal-01535192
https://hal.univ-antilles.fr/hal-01535192/document
https://hal.univ-antilles.fr/hal-01535192/file/CARIOU_REE_21_Janv_2015_.pdf

Éditeur(s) : HAL CCSD Taylor & Francis Open
Résumé : International audience
Our recent findings indicate that cells exposed to transmembrane (m-CD95L) or metalloprotease-cleaved CD95L (cl-CD95L) undergo a localized Ca(2+)entry that not only inhibits the initial steps of the CD95-mediated apoptotic signal but also promotes cell motility. Based on recent findings published on the non-apoptotic signals induced by CD95, we discuss how m-CD95L and cl-CD95L diverging by their stoichiometry could both contribute to the immune response by first recruiting activated T lymphocytes in the inflamed area and later by eliminating infected and transformed cells.
ISSN: 1942-0889

hal-00873761
https://hal.archives-ouvertes.fr/hal-00873761
https://hal.archives-ouvertes.fr/hal-00873761/document
https://hal.archives-ouvertes.fr/hal-00873761/file/The_CD95_signaling_pathway-accepted.pdf
DOI : 10.4161/cib.18888

Éditeur(s) : HAL CCSD Springer Verlag (Germany) Springer Verlag
Résumé : International audience
Apoptosis has emerged as a fundamental process important in tissue homeostasis, immune response, and during development. CD95 (also known as Fas), a member of the tumor necrosis factor receptor (TNF-R) superfamily, has been initially cloned as a death receptor. Its cognate ligand, CD95L, is mainly found at the plasma membrane of activated T-lymphocytes and natural killer cells where it contributes to the elimination of transformed and infected cells. According to its implication in the immune homeostasis and immune surveillance, and since several malignant cells of various histological origins exhibit loss-of-function mutations, which cause resistance towards the CD95-mediated apoptotic signal, CD95 has been classified as a tumor suppressor gene. Nevertheless, this assumption has been recently challenged, as in certain pathophysiological contexts, CD95 engagement transmits non-apoptotic signals that promote inflammation, carcinogenesis or liver/peripheral nerve regeneration. The focus of this review is to discuss these apparent contradictions of the known function(s) of CD95.
ISSN: 1420-682X

hal-00682466
https://hal.archives-ouvertes.fr/hal-00682466
DOI : 10.1007/s00018-011-0866-4
PUBMED : 22042271

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Based on new photographs of the wound, Tirard et al. (2015) tried to demonstrate that the shark involved in a fatal attack on a human in Lifou in 2007 had homodont teeth and that it sawed the femur instead of directly cutting it, promoting the hypothesis that it was a tiger shark instead of a white shark. They also contested the data provided by the direct witness of the attack about the behaviour of the shark, specific to this former species. The evidences they provide are not convincing and, based on the absence of tissue loss and description of a jumping behaviour, we still believe that it was a single bite-and-spit attack by a white shark.
ISSN: 1752-928X

hal-01310536
https://hal-univ-perp.archives-ouvertes.fr/hal-01310536
DOI : 10.1016/j.jflm.2016.03.004

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Gravitropism is necessary for plants to control the orientation of their axes while they grow in height. In woody plants, stem re-orientations are costly because they are achieved through diameter growth. The functional diversity of gravitropism was studied to check if the mechanisms involved and their efficiency may contribute to the differentiation of height growth strategies between forest tree species at the seedling stage. Seedlings of eight tropical species were grown tilted in a greenhouse, and their up-righting movement and diameter growth were measured during three months. Morphological, anatomical and biomechanical traits were measured at the end of the survey. Curvature analysis was used to analyse the up-righting response along the stems. Variations in stem curvature depend on diameter growth, size effects, the increase in self-weight, and the efficiency of the gravitropic reaction. A biomechanical model was used to separate these contributions. Results showed that (1) gravitropic movements were based on a common mechanism associated to similar dynamic patterns, (2) clear differences in efficiency (defined as the change in curvature achieved during an elementary diameter increment for a given stem diameter) existed between species, (3) the equilibrium angle of the stem and the anatomical characters associated to the efficiency of the reaction also differed between species, (4) the differences in gravitropic reaction were related to the light requirements: heliophilic species, compared to more shade-tolerant species, had a larger efficiency and an equilibrium angle closer to vertical. This suggests that traits determining the gravitropic reaction are related to the strategy of light interception and may contribute to the differentiation of ecological strategies promoting the maintenance of biodiversity in tropical rain forests.
ISSN: 0022-0957

hal-00797023
https://hal.archives-ouvertes.fr/hal-00797023
https://hal.archives-ouvertes.fr/hal-00797023/document
https://hal.archives-ouvertes.fr/hal-00797023/file/Functional_diversity_gravitropic_Almeras_al.pdf

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Pre-association of CD95 at the plasma membrane is mandatory for efficient death receptor signaling. This homotrimerization occurs through self-association of an extracellular domain called the pre-ligand assembly domain (PLAD). Using novel molecular and cellular tools, we confirmed that CD95-PLAD is necessary to promote CD95 multimerization and plays a pivotal role in the transmission of apoptotic signals. However, while a human CD95 mutant deleted of the previously described PLAD domain (amino acids 1 to 66) fails to interact with its wild-type counterpart and trigger autonomous cell death, deletion of amino acids 1 to 42 does not prevent homo- or hetero (human/mouse)-oligomerization of CD95, and thus does not alter transmission of the apoptotic signal. Overall, these findings indicate that the region between amino acids 43 to 66 corresponds to the minimal motif involved in CD95 homotypic interaction and is necessary to convey an efficient apoptotic signal. Interfering with this PLAD may represent a new therapeutic strategy for altering CD95-induced apoptotic and non-apoptotic signals.
ISSN: 1932-6203

hal-00873708
https://hal.archives-ouvertes.fr/hal-00873708
https://hal.archives-ouvertes.fr/hal-00873708/document
https://hal.archives-ouvertes.fr/hal-00873708/file/Precise_Mappingsur the_CD95-accepted.pdf
DOI : 10.1371/journal.pone.0046236

Éditeur(s) : Cnexo
Résumé : Voici un an déjà le dépôt du Programme d'action à Moyen Terme que m'avaient demandé les Ministres, marquait le départ d'une réflexion intense au niveau gouvernemental sur l'océanologie. Témoignage d'un intérêt précieux pour la mise en valeur d'océans à la fois si proches et si lointains par la faiblesse de nos connaissances et de nos moyens d'investigation, cette réflexion a clarifié le cadre dans lequel nous devons travailler. Notre rôle, notre mission en ressortent mieux. Nous ne sommes pas un ensemble de recherche-développement comme les autres parce que nous sommes, parce que vous êtes d'abord au service de quatre missions nationales indissociables qui nous sont confiées: 1. animer, orienter, développer en liaison avec les utilisateurs finaux l'effort de recherche exploratoire national, c'est-à-dire cette recherche de pointe qui doit associer les finalités de connaissance et celles des applications sur des problématiques de portée générale et avec des visées qui peuvent être à relativement long terme; 2. permettre à la communauté scientifique de disposer des meilleurs outils, des meilleurs équipements lourds pour poursuivre un effort dont l'efficacité, la productivité scientifique doivent être accrues fortement en raison de son coût élevé pour la Nation. A cette mission se rattache celle de favoriser le développement des technologies de base utile à la pénétration, à la mesure et à la valorisation du milieu marin lato sensu; 3. être l'outil privilégié de la promotion d'une approche intégrée pluridisciplinaire et intersectorielle de l'effort océanologique; 4. S'assurer du meilleur transfert possible à l'économie des résultats de l'effort public de recherche en le facilitant en cas de besoin, notamment en encourageant l'effort privé et en s'y associant. [OCR NON CONTRÔLE]
La Lettre du Cnexo (Cnexo), 1980-04 , N. 1 , P. 1-6

Droits : Cnexo - Ifremer
http://archimer.ifremer.fr/doc/1980/publication-4776.pdf
http://archimer.ifremer.fr/doc/00000/4776/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
From 15 to 17 June 2011, a dedicated workshop was held on the subject of in vitro models for mammalian spermatogenesis and their applications in toxicological hazard and risk assessment. The workshop was sponsored by the Dutch ASAT initiative (Assuring Safety without Animal Testing), which aims at promoting innovative approaches toward toxicological hazard and risk assessment on the basis of human and in vitro data, and replacement of animal studies. Participants addressed the state of the art regarding human and animal evidence for compound mediated testicular toxicity, reviewed existing alternative assay models, and brainstormed about future approaches, specifically considering tissue engineering. The workshop recognized the specific complexity of testicular function exemplified by dedicated cell types with distinct functionalities, as well as different cell compartments in terms of microenvironment and extracellular matrix components. This complexity hampers quick results in the realm of alternative models. Nevertheless, progress has been achieved in recent years, and innovative approaches in tissue engineering may open new avenues for mimicking testicular function in vitro. Although feasible, significant investment is deemed essential to be able to bring new ideas into practice in the laboratory. For the advancement of in vitro testicular toxicity testing, one of the most sensitive end points in regulatory reproductive toxicity testing, such an investment is highly desirable.
ISSN: 0890-6238

hal-00876979
https://hal.archives-ouvertes.fr/hal-00876979
DOI : 10.1016/j.reprotox.2013.04.004
PUBMED : 23612449

Éditeur(s) : HAL CCSD Wiley-VCH Verlag
Résumé : International audience
Proteomic profiling plays a decisive role in the elucidation of molecular signatures representative of a specific clinical context. MuStem cell-based therapy represents a promising approach for clinical applications to cure Duchenne Muscular Dystrophy (DMD). To expand our previous studies collected in the clinically relevant DMD animal model, we decided to investigate the skeletal muscle proteome four months after systemic delivery of allogenic MuStem cells. Quantitative proteomics with isotope-coded protein labelling (ICPL) was used to compile quantitative changes in the protein expression profiles of muscle in transplanted Golden Retriever Muscular Dystrophy (GRMD) dogs as compared to GRMD dogs. A total of 492 proteins were quantified, including 25 that were overrepresented and 46 that were underrepresented after MuStem cell transplantation. Interestingly, this study demonstrates that somatic stem cell therapy impacts on the structural integrity of the muscle fascicle by acting on fibres and its connections with the extracellular matrix. We also show that cell infusion promotes protective mechanisms against oxidative stress and favours the initial phase of muscle repair. This study allows us to identify putative candidates for tissue markers that might be of great value in objectively exploring the clinical benefits resulting from our cell-based therapy for DMD.
ISSN: 1615-9853

Droits : info:eu-repo/semantics/OpenAccess
hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030/document
https://hal.archives-ouvertes.fr/hal-01332030/file/Quantitative%20proteome%20profiling%20of%20dystrophic%20dog%20skeletal%20muscle_accepted.pdf
DOI : 10.1002/pmic.201600002

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We conducted triaxial compression experiments to investigate the effects of pressure, temperature and strain rate on the rheology of talc rocks. The tests were carried out at T from 25 to 860 degrees C, P from 0.1 to 300 MPa, and <(epsilon) over dot> from 0.3 to 30 x 10-5 s(-1). In addition to being very weak, there are other important differences between the mechanical behavior of talc and that of most other silicates. Deformation at all temperatures up to dehydration was accommodated by a combination of crystal plasticity, frictional sliding, and cataclasis. A transition from localized to distributed deformation occurred at P = 300 MPa and T = 400 degrees C, but this transition is ill-defined as both distributed and localized deformation were observed at P = 300 MPa and T = 600 degrees C. Unlike most silicates, both the coefficient of internal friction and the coefficient of (sliding) friction are very low and nearly equal to each other. Temperature enhances plastic deformation (kinking), and inter- and intra-granular microcracks are observed parallel to the (001) planes at all conditions. Voids are created by delamination along (001) planes at kink-band boundaries. Full crystal plasticity was not achieved under any of the conditions tested, i.e., the von Mises criterion was never satisfied. However, the strength of the aggregates remained much less than the confining pressure. These unusual properties are likely a manifestation of the pronounced mechanical anisotropy of talc at the grain scale. At T >= 750 degrees C dehydration is observed, but only along shear zones. These results suggest that feedbacks between deformation and reaction kinetics could control fluid flow in fault zones. The presence of talc can promote significant weakening and strain localization in the oceanic lithosphere, the subducting plate and the overlying mantle wedge. (c) 2008 Elsevier B.V. All rights reserved.
ISSN: 0012-821X

hal-00492706
https://hal.archives-ouvertes.fr/hal-00492706
DOI : 10.1016/j.epsl.2008.02.004

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
Construction works in healthcare establishments produce airborne fungal spores and considerably increase the risk of exposure of immunosuppressed patients. It is necessary to reinforce protective measures, or even to implement specific precautions, during this critical phase. The aim of these precautions is to protect both those areas, which are susceptible to dust, and patients at risk of a fungal infection particularly invasive aspergillosis. When construction works are planned in healthcare establishments, the first step consists in the characterisation of the environmental fungal risk and the second one in proposing risk management methods. It is then essential to establish impact indicators in order to evaluate the risk management precautions applied. The working group promoted by the French societies of medical mycology and hospital hygiene (SFMM & SF2H) details here both environmental and epidemiological impact indicators that can be used.
ISSN: 1156-5233

hal-00759179
https://hal.archives-ouvertes.fr/hal-00759179
DOI : 10.1016/j.mycmed.2012.01.003
PUBMED : 23177816

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : À la fin du xixe siècle, la fabrique horlogère de Genève est confrontée à l’arrivée de montres reprenant abusivement ses marques et le nom de Genève. En réaction, les horlogers, les marchands et les pouvoirs publics mettent en place un dispositif pour protéger ce secteur économique. L’une des solutions retenues est d’identifier les montres faites Genève en certifiant leur qualité. En 1886, la création d’un poinçon de l’État et de la République de Genève est le premier label associant les savoir-faire d’un territoire à la qualité technique de ses produits. A l’origine défensif, le poinçon fait la promotion de produits de haut de gamme caractérisés par une bonne facture, une fiabilité et leur origine, marqueur du luxe des montres de Genève.
At the end of the 19th century, the watchmakers of Geneva are confronted to the arrival of products retaking abusively its brands and the name of Geneva. In response, watchmakers, retailers and public actors decided to protect this economic sector. One of the solutions picked up was to identify Geneva-made watches by certifying their quality. In 1886, the creation of a hallmark of the State and the Republic of Geneva is the first label associating the expertise of a territory with the technical quality of its products. Originally defensive, the hallmark promotes high-quality products characterized by a good invoice, a reliability and their origin, a branding of the luxury of Geneva watches.
Genève

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.7370
http://journals.openedition.org/etudescaribeennes/7370

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Epithelial-to-mesenchymal transition (EMT) promotes cell motility, which is important for the metastasis of malignant cells, and blocks CD95-mediated apoptotic signaling triggered by immune cells and chemotherapeutic regimens. CD95L, the cognate ligand of CD95, can be cleaved by metalloproteases and released as a soluble molecule (cl-CD95L). Unlike transmembrane CD95L, cl-CD95L does not induce apoptosis but triggers cell motility. Electron paramagnetic resonance was used to show that EMT and cl-CD95L treatment both led to augmentation of plasma membrane fluidity that was instrumental in inducing cell migration. Compaction of the plasma membrane is modulated, among other factors, by the ratio of certain lipids such as sphingolipids in the membrane. An integrative analysis of gene expression in NCI tumor cell lines revealed that expression of ceramide synthase-6 (CerS6) decreased during EMT. Furthermore, pharmacological and genetic approaches established that modulation of CerS6 expression/activity in cancer cells altered the level of C16-ceramide, which in turn influenced plasma membrane fluidity and cell motility. Therefore, this study identifies CerS6 as a novel EMT-regulated gene that has a pivotal role in the regulation of cell migration.Oncogene advance online publication, 17 March 2014; doi:10.1038/onc.2014.55.
ISSN: 0950-9232

hal-01063558
https://hal.archives-ouvertes.fr/hal-01063558
https://hal.archives-ouvertes.fr/hal-01063558/file/Down-regulation%20of%20ceramide_accepted.pdf
DOI : 10.1038/onc.2014.55
PUBMED : 24632610