Evaluation of apoptotic- and autophagic-related protein expressions before and after IVM of fresh, slow-frozen and vitrified pre-pubertal mouse testicular tissue Auteur(s) : Dumont, L. Chalmel, F. Oblette, A. Berby, B. Rives, A. Duchesne, V. Rondanino, C. Rives, N. Auteurs secondaires : Gamétogenèse et Qualité des Gamètes (GQG) ; Université de Rouen Normandie (URN) ; Normandie Université (NU) - Normandie Université (NU) - Université Lille 2 - Faculté de Médecine Institute for Research and Innovation in Biomedicine (IRIB) ; Université de Rouen Normandie (URN) ; Normandie Université (NU) - Normandie Université (NU) - Institut National de la Santé et de la Recherche Médicale (INSERM) Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Éditeur(s) : HAL CCSD Résumé : International audience STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest. Molecular Human Reproduction hal-01647081 https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081 DOI : 10.1093/molehr/gax054 PUBMED : 29040674 | Partager |
1898 - Rough Riders Auteur(s) : Burgert Brothers, 1917- ( Photographer ) Résumé : At the outbreak of war with Spain in 1898, the U.S. Army was very small in numbers. This situation necessitated an immediate call for volunteers. President McKinley's appeal was overwhelmingly answered by a generation that had grown up in the shadow of their elders' Civil War glory.
One group answering the call was the First Regiment of the U.S. Cavalry Volunteers, headed by Colonel Leonard Wood, a distinguished army doctor and Medal of Honor recipient. The regiment was actually the brainchild of Theodore Roosevelt, assistant secretary of the navy and Wood's friend. Roosevelt, realizing his own lack of military experience, suggested Wood for the command.
The Rough Riders, as the regiment was soon known, comprised 1,250 men, including cowboys, Native Americans and eastern college athletes. Despite their dissimilarities, they were in excellent physical condition'a stark contrast to most of the other volunteer contingents.
The Rough Riders departed from Tampa in mid-June without their horses. They landed at Daiquiri on June 22 and two days later served with distinction in a battle at Las Guásimas.
Immediately prior to the conflict at San Juan, Colonel Wood was promoted to another field command, enabling Roosevelt as a full colonel to take command of the Rough Riders. On July 1, Roosevelt, having secured a horse, led his forces in a charge up Kettle Hill outside of Santiago. They achieved their goal and later in the day participated in the victory at San Juan Hill.
By seizing these heights, American guns commanded the harbor at Santiago. The Spanish position was imperiled and an abortive attempt by the Spanish navy to escape from the harbor was halted with devastating results. Spanish land forces surrendered shortly thereafter.
Roosevelt urged the Rough Riders' swift evacuation, fearing the continuing spread of disease. They returned to Montauk, Long Island, where they were held in quarantine before being mustered out in September.
More than one-third of the Rough Riders were casualties in the Spanish-American War, a fact that has led some observers to criticize Roosevelt for unnecessary risk-taking. Nevertheless, the Rough Riders became heroes to the American public and Roosevelt emerged as a major national figure. (Funding) Funded in part by the Institute for Museum and Library Services (IMLS), Ephemeral Cities Project. Tampa |z 1271000 |2 ceeus Hillsborough County |z 12057 |2 ceeus United States of America -- Florida -- Hillsborough County -- Tampa United States Droits : All rights reserved. 2005. D20-012 | Partager |
Carnival costumes on exhibit in the National Museum and Art Gallery of Trinidad and Tobago ; The Bryant Slides Collection ; The Bryant Slides Collection, Trinidad Auteur(s) : Unknown ( Photographer ) Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location. In Trinidad and Tobago, carnival celebrations begin after Christmas and peak during the Monday and Tuesday preceding Ash Wednesday. Costumed masqueraders and festival revelers partake in the two-day street parade, generally accompanied by soca, calypso, and steel pan music, before beginning the Lenten season. The National Museum and Art Gallery hosts a permanent ongoing exhibit of masks and costumes from past carnivals. This image shows the full view of two costumes. The partial view of two more costumes can be seen as well. One of the costumes is decorated with feathered props and fans. The costume also features geometric shapes and a stylized bird’s head, with the opening for the wearer at the base of the bird’s neck. Another costume is decorated with hands accompanied by a feathered head gear featuring a stylized human face. Slide labeled Trin. 11. Trinidad and Tobago -- South America -- Port of Spain, Trinidad Trinidad and Tobago -- Caribbean region -- Port of Spain, Trinidad Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu CFM1972_01a http://ufdc.ufl.edu/AA00031201/00001 | Partager Voir aussi Port of Spain (Trinidad and Tobago) -- Social life and customs Port of Spain (Trinidad and Tobago) -- Description and travel Museum exhibits -- Trinidad and Tobago -- Port of Spain -- 1960-1970 Headgear -- Trinidad and Tobago -- Port of Spain -- 1960-1970 Carnival costume -- Trinidad and Tobago -- Port of Spain -- 1960-1970 |
The History of Sandford and Merton ; Sandford and Merton Auteur(s) : Day, Thomas, 1748-1789 Thomas Nelson & Sons ( Publisher ) Éditeur(s) : T. Nelson and Sons T. Nelson and Sons ( London, Edinburgh, New York ) Résumé : (Statement of Responsibility) by Thomas Day. Added title-page and plates printed in colors. England -- London Scotland -- Edinburgh United States -- New York -- New York Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide. ALK0532 14341781 002248807 | Partager Voir aussi Boys -- Conduct of life -- Juvenile fiction ( lcsh ) Conduct of life -- Juvenile fiction ( lcsh ) Boys -- Education -- Juvenile fiction ( lcsh ) Friendship -- Juvenile fiction ( lcsh ) Natural history -- Juvenile fiction ( lcsh ) Country life -- Juvenile fiction ( lcsh ) Courage -- Juvenile fiction ( lcsh ) Friendship -- Juvenile literature ( lcsh ) Prejudices -- Juvenile literature ( lcsh ) Dialogues -- 1872 ( rbgenr ) |
Stone wall, Havana ; Manuel R. Bustamante Photograph Collection. Résumé : Written on verso: "Said to be 400. (now in front of the new Presidential Palace, it marks the city limits in the first days of Havana city)" 1910-1930 CHC5017 Havana (Cuba) Droits : http://merrick.library.miami.edu/digitalprojects/copyright.html chc50170001180001001 1442 | Partager |
A house for rent in Cooper’s Town, Great Abaco, Bahamas ; The Bryant Slides Collection ; The Bryant Slides Collection, Bahamas Auteur(s) : Unknown ( Photographer ) Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location. Two houses, one for rent, in Cooper’s Town, Great Abaco, Bahamas. Slide labeled House Cooperstown for Rent $15/day April '65. Bahamas -- North America -- Cooper’s Town, Great Abaco Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu CFM1972_01a Sheet 11:10 http://ufdc.ufl.edu/AA00028957/00001 | Partager |
Boats fill the Hillsbourough River in front of the Plant Park and the Tampa Bay Hotel. Auteur(s) : Burgert Brothers, 1917- ( Photographer ) Résumé : When Henry B. Plant brought the railroad to Tampa in 1884, he opened the door for many businesses to locate and flourish here. The railroad was at the center of the city's economy. From downtown it went to Port Tampa where one could board a Plant steamship to Jamaica, Cuba, New Orleans, Bermuda or other destinations. A spur of the rail line brought the train up to the west front of the hotel so guests could depart the train and walk directly into the lobby.
The Tampa Bay Hotel cost approximately 2.5 million dollars to build and $500,000 to furnish. Consisting of 511 rooms, some of which were suites of 3-7 rooms, it provided a degree of luxury never before seen in Tampa. Most of the rooms had their own baths and all had electricity and telephones. In addition, guests were surrounded by a varied collection of furniture, porcelains, Venetian-style mirrors, and sculptures gathered by Mr. and Mrs. Plant in their travels in Europe.
The Tampa Bay Hotel opened in 1891 amid wide fanfare and celebration. Henry Plant's dream was now a reality. The newspapers of the day described it as "brightly illuminated, filled with sumptuous decorations, thrilling music and graced with turrets, domes and minarets towering heavenward and
glistening in the sun." It was a Victorian palace.
The hotel was a lively place with balls, tea parties, and organized hunts during the winter social season. It was open seasonally from December to April in the 1890's. Once a guest arrived at the hotel, he needed to be entertained. Guests went wild game hunting as well as fresh and salt-water fishing. Water sports were also included like sailing, rowing, and canoeing. For the less adventuresome, there were bicycles, carriages, and rickshaws to ride you around the property. The hotel also offered golf, tennis, shuffleboard, billiards, and even a racetrack. (Funding) Funded in part by the Institute for Museum and Library Services (IMLS), Ephemeral Cities Project. Tampa |z 1271000 |2 ceeus Hillsborough County |z 12057 |2 ceeus United States of America -- Florida -- Hillsborough County -- Tampa Droits : All rights reserved. 2005. D29-009 | Partager Voir aussi Hotel ( Building, Structures ) ( nmc ) Hotels ( lctgm ) Tampa Bay Hotel (West Lafayette and Hyde Park Avenue) ( lctgm ) Plant Park (West Lafayette Street opp Hyde Park Avenue) ( lctgm ) Rivers ( lctgm ) Hillsborough River ( lctgm ) Parks ( lctgm ) Piers and wharves ( lctgm ) Minarets ( lctgm ) Tampa Bay Hotel (Tampa, Fla.) -- Pictorial works -- 20th century ( lcsh ) |
Trinidad and Tobago revised ordinances, 1950 ; Laws, etc. (Trinidad and Tobago revised ordinances, 1950 Auteur(s) : Trinidad Maingot, Elliot Francis, 1891- Éditeur(s) : Printed by C. F. Roworth, Govt. printers Printed by C. F. Roworth, Govt. printers ( London ) Résumé : (Statement of Responsibility) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. Cover title: Laws of Trinidad and Tobago, 1950. "Contains the ordinances of the colony in force on the 31st day of December, 1950, exclusive of those reserved by ordinance no.23 of 1949 and by subsequent proclamations." Trinidad and Tobago 000255255 28706053 ABC5972 | Partager |
Trinidad and Tobago revised ordinances, 1950 Auteur(s) : Trinidad Maingot, Elliot Francis, 1891- Éditeur(s) : Printed by C.F. Roworth, Govt. printers, Printed by C.F. Roworth, Govt. printers Printed by C.F. Roworth, Govt. printers, Printed by C.F. Roworth, Govt. printers ( London ) Résumé : (Internal Comments) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. (Statement of Responsibility) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. Cover title: Laws of Trinidad and Tobago, 1950. "Contains the ordinances of the colony in force on the 31st day of December, 1950, exclusive of those reserved by ordinance no. 23 of 1949 and by subsequent proclamations." Trinidad and Tobago Trinidad ABC5972 02958982 000255255 54017719 | Partager |
Trinidad and Tobago revised ordinances, 1950 Auteur(s) : Trinidad Maingot, Elliot Francis, 1891- Éditeur(s) : Printed by C.F. Roworth, Govt. printers, Printed by C.F. Roworth, Govt. printers Printed by C.F. Roworth, Govt. printers, Printed by C.F. Roworth, Govt. printers ( London ) Résumé : (Internal Comments) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. (Statement of Responsibility) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. Cover title: Laws of Trinidad and Tobago, 1950. "Contains the ordinances of the colony in force on the 31st day of December, 1950, exclusive of those reserved by ordinance no. 23 of 1949 and by subsequent proclamations." Trinidad and Tobago Trinidad ABC5972 02958982 000255255 54017719 | Partager |
Bay Street in New Providence, Bahamas ; The Bryant Slides Collection ; The Bryant Slides Collection, Bahamas Nassau Hope Town Auteur(s) : Unknown Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Bay Street on a crowded day with many automobiles and a horse drawn surrey carriage. The British Colonial Hotel can be seen in the distance. Slide labeled Bryant Nassau Mar .'46. Bahamas -- North America -- Nassau, New Providence Island Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu CFM1972_01a Sheet 17:3 http://ufdc.ufl.edu/AA00029513/00001 | Partager |
Planters' Punch Auteur(s) : Herbert G. deLisser Éditeur(s) : Planters' Punch Planters' Punch ( [Kingston: Jamaica] ) Résumé : (Biographical) From Wikipedia for H. G. de Lisser, from 29 June 2013: Herbert George de Lisser CMG (9 December 1878 - 19 May 1944) was a Jamaican journalist and author. He has been called "one of the most conspicuous figures in the history of West Indian literature". De Lisser was born in Falmouth, Jamaica, and attended William Morrison's Collegiate School in Kingston. He started work at the Institute of Jamaica at the age of 14. Three years later he joined the Jamaica Daily Gleaner, of which his father was editor, as a proofreader, and two years later became a reporter on the Jamaica Times. In 1903, De Lisser became assistant editor of the Gleaner and was editor within the year. He wrote several articles for the paper every day. He also produced a novel or non-fiction book every year, beginning in 1913 with Jane: A Story of Jamaica, significant for being the first West Indian novel to have a central black character. Another famous novel of his, The White Witch of Rosehall (1929), is linked to a legend of a haunting in Jamaica. De Lisser also wrote several plays. In December 1920 he began publishing an annual magazine, Planters' Punch. De Lisser devoted much time and effort to the revival of the Jamaican sugar industry and represented Jamaica at a number of sugar conferences around the world. He was also general secretary of the Jamaica Imperial Association, honorary president of the Jamaica Press Association, and chairman of the West Indian section of the Empire Press Union. He was appointed Companion of the Order of St Michael and St George (CMG) in the 1920 New Year Honours. Jamaica Droits : All rights reserved by the source institution. P57 http://ufdc.ufl.edu/AA00004645/00003 | Partager |
Cubans celebrate Santa Barbara feast day Auteur(s) : Reinke, Bill ( Photographer ) Résumé : Photo date stamped on verso: December 5, 1975. (Preferred Citation) Miami News Collection, HistoryMiami, 1995-277-3100. (Biographical) Saint Barbara's feast day is December 4. Miami, Florida Droits : All rights reserved by the source institution. 1995-277-3098 http://ufdc.ufl.edu/AA00015770/00001 | Partager |
The Entrance Gates, Plant Park, the the Tampa Bay Hotel with it's unique minarets. Auteur(s) : Burgert Brothers, 1917- ( Photographer ) Résumé : When Henry B. Plant brought the railroad to Tampa in 1884, he opened the door for many businesses to locate and flourish here. The railroad was at the center of the city's economy. From downtown it went to Port Tampa where one could board a Plant steamship to Jamaica, Cuba, New Orleans, Bermuda or other destinations. A spur of the rail line brought the train up to the west front of the hotel so guests could depart the train and walk directly into the lobby.
The Tampa Bay Hotel cost approximately 2.5 million dollars to build and $500,000 to furnish. Consisting of 511 rooms, some of which were suites of 3-7 rooms, it provided a degree of luxury never before seen in Tampa. Most of the rooms had their own baths and all had electricity and telephones. In addition, guests were surrounded by a varied collection of furniture, porcelains, Venetian-style mirrors, and sculptures gathered by Mr. and Mrs. Plant in their travels in Europe.
The Tampa Bay Hotel opened in 1891 amid wide fanfare and celebration. Henry Plant's dream was now a reality. The newspapers of the day described it as "brightly illuminated, filled with sumptuous decorations, thrilling music and graced with turrets, domes and minarets towering heavenward and
glistening in the sun." It was a Victorian palace.
The hotel was a lively place with balls, tea parties, and organized hunts during the winter social season. It was open seasonally from December to April in the 1890's. Once a guest arrived at the hotel, he needed to be entertained. Guests went wild game hunting as well as fresh and salt-water fishing. Water sports were also included like sailing, rowing, and canoeing. For the less adventuresome, there were bicycles, carriages, and rickshaws to ride you around the property. The hotel also offered golf, tennis, shuffleboard, billiards, and even a racetrack. (Funding) Funded in part by the Institute for Museum and Library Services (IMLS), Ephemeral Cities Project. Tampa |z 1271000 |2 ceeus Hillsborough County |z 12057 |2 ceeus United States of America -- Florida -- Hillsborough County -- Tampa Lafayette Street Droits : All rights reserved. 2005. D20-008 | Partager |
Forum with Parliament Members : Students Against Sexual Orientation Discrimination ; Audio Recordings of the Proceedings Auteur(s) : Caribbean IRN Résumé : From the PNCR, GAP MPs free to vote conscience on gay rights bill
By Johann Earle
Stabroek News
June 11, 2003
The PNCR will allow its members to vote their conscience on the controversial Constitution (Amendment) Act of 2001 which seeks to prevent discrimination against persons on the basis of their sexual orientation and other grounds.
A number of religious organisations are opposed to the passage of the bill because it includes sexual orientation as one of the grounds. They are concerned that it could lead to a legalisation of homosexual relations and demands for recognition of gay marriages among other things.
PNCR Member of Parliament (MP) Vincent Alexander told a forum at the National Library on Saturday, that the Bill did not seek to legalise homosexuality, but to ensure that persons would not be discriminated against based on their sexual preferences.
He was one of two parliamentarians who showed up - the other being PNCR member, Myrna Peterkin.
The forum was organised by Students Against Sexual Orientation Discrimination (SASOD), a group comprising fifteen students from the University of Guyana which was formed about two weeks ago.
Alexander expects the vote on the bill to come up before the National Assembly in a matter of months.
Paul Hardy, Leader of the Guyana Action Party (GAP), told Stabroek News that his party would not be using the so-called parliamentary whip. He added that GAP took a decision that every member should vote according to his or her own conscience. “We have no right to deny the rights of others based on sexual orientation. [The Bill] will guarantee rights to the homosexual.” GAP is in Parliament as part of an alliance with the Working People’s Alliance. GAP/WPA has two MPs.
The bill was met with rejection from some members of the religious community in 2001 and as a result of this, the President did not assent to it.
In a statement on Monday, the Central Islamic Organisa-tion of Guyana (CIOG) said that it stood in firm opposition to the Bill. The CIOG says the general purpose of the bill may be commendable and that the organisation’s objection is not based on a willingness to promote discrimination. Rather, the CIOG said, it was based on the fact that specific legal protection on the basis of sexual orientation without definition or qualification gives tacit legitimacy to practices which are considered criminal in Islam. “It is foreseeable that such a legal nod of approval (subtle as it may be) of these practices may pave the way for greater social (or even legal) acceptability in the future which, from the perspective of all Muslims including those in Guyana, is an undesirable and sinful outcome,” the CIOG statement said.
At Saturday’s event, Muslim teacher Moulana Mohamed Ali Zenjibari spoke of instances of abuse, discrimination and harsh penalties meted out to gay and lesbian persons in countries such as Iran and Saudi Arabia and noted that the Quran did not sanction such punishments for homosexual behaviour.
President of the CIOG, Fazeel Ferouz told Stabroek News that a meeting to discuss a strategy with regards to the bill was planned for tomorrow with various religious groups.
Stabroek News tried to get a comment on the issue from the PPP/C but to no avail.
ROAR leader, Ravi Dev said that his party was now having discussions on the issue. ROAR feels that it is an important question which has to do with morality and should be discussed across the country. He added legislators had to be in tune with their constituents on the issue.
SASOD is lobbying for the legislation through the sensitisation of MPs.
Keimo Benjamin, a law student at UG, gave a presentation based on the jurisprudential aspects of the discussion on sexual orientation. He argued that morality should not be the only guiding principle on which to base the laws. Sexual activities between two consenting male adults in private could not be equated with a violation of a person’s rights, he said, making the point that the thrust of his presentation was not whether homosexuality was wrong, but whether it violated the rights of others. He said that the attitudes of some towards this subject were based on preconceived notions and prejudices. He cited studies to show that the suppression of certain perceived deviant sexual impulses in persons might do more harm than good. One Harvard University study of teens who said they were gay indicated that those teens were three times more likely to commit suicide.
Vidyaratha Kissoon, of Help and Shelter, in his contribution on Saturday, expressed his displeasure at the low turnout at the forum and urged the parliamentarians who showed up to take the message to their colleagues. He noted that because of homophobia, the numerical minority was terrified of speaking out against instances of discrimination. Gays and lesbians in Guyana were subjected to ridicule and abuse, and walk the streets at night not looking for sex necessarily, but for the companionship of persons who empathise with them.
During his presentation, Joel Simpson, another member of SASOD, outlined a number of changes made within national jurisdictions that had international implications. One such crucial change was South Africa’s 1996 adoption of a new constitution, making that country the first in the world to expressly include sexual orientation as a prohibited ground of discrimination. It was the first time a developing country had taken the lead with respect to the rights of sexual minorities.
He also said that according to Douglas Sanders, a Canadian jurist, the rights of homosexual, bisexual or transsexual men and women had never been officially recognised by the United Nations, despite the fact that international laws on the issue began to emerge at the close of the Second World War.
Simpson said that under Article 170 (5), as amended by Section 8 of the Constitution (Amendment) (No. 4) Act 2000, the President is required to assent to any bill which is returned by the National Assembly unaltered after a two-thirds majority within 90 days of its presentation to him. To the parliamentarians present, Simpson stressed that the onus was now on them to adequately represent their constituents which include lesbian, gay, bisexual and transsexual Guyanese.
http://www.landofsixpeoples.com/news301/ns306115.htm
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Vote on sexual orientation should be a matter of conscience - MP Alexander
By Esther Elijah
Guyana Chronicle
June 8, 2003
PEOPLE’S National Congress (PNC/R) Member of Parliament Mr. Vincent Alexander, said the Opposition will vote on “conscience” when the piece of legislation on sexual orientation is again brought before the National Assembly.
“With specific reference to my party, when this Bill gets back to the Parliament in the spirit of the arguments here, we will not use the `whip’. Our party will not say we have to vote for the provision (in the Sexual Orientation Bill). We will allow our members to vote as a matter of conscience,” he told participants gathered in the Conference Room of the National Library.
“We feel this is a matter of conscience. You may end up with a collective position but you have to deal with us individually,” Alexander said at a poorly attended public consultation aimed at gaining support for sexual orientation to be considered a fundamental right in Guyana.
Alexander, one of the main persons who sat on the Constitutional Reform Commission that addressed this controversial clause, said the legislation was not meant to legalise homosexual activities in Guyana.
“It was intended to ensure that persons who have an orientation - a way of thinking - which may or may not lead to a certain activity, to not be discriminated against, in terms of their rights,” he explained.
Alexander noted that very often, discussions on the sexual orientation provision in the Bill have led to seepages into other areas where debates centre on the “right to be homosexual.”
“I am saying the Bill does not comment on that… However, law is peculiar, especially in a Common Law system. Once you venture out and change the law, very often you open other windows which we cannot definitively say exist or does not exist in advance,” he said.
“The fear of some people is that the legislators might say one thing and the Courts will eventually say something else. While some people can’t argue against the law, per say, they will say this has opened a window of opportunity not meant to be opened. So, it is better to stay without a window than open it and then have a possibility of something you didn’t intend to happen - happening sometime in the future.”
Alexander added: “I want to say I’ve found this activity to have been rich from the perspective of the amount of research which was done by student (speakers). Without any comment as to whether I agree with the arguments, I would wish that much more research on other issues be done by students, and that at the University (of Guyana) students would find it convenient to have forums on other issues, with the same depth of research for their own intellectual development.”
Alexander and other Opposition M.P, Lurlene Nestor were the only three Parliamentarians in attendance at the session organised by the recently formed `Students Against Sexual Orientation Discrimination’ (SASOD), a group that has a membership of 15 mostly University of Guyana (UG) students from various faculties.
SASOD was established two weeks ago.
With the exception of members of the press, the consultation only managed to attract 11 persons, a handful of who were SASOD members.
While congratulating the students for an “insightful” presentation, Nestor pointed out that whether or not she chooses to agree with their position is “another issue” and she is entitled to her “own view”.
“The issue of sexual orientation is very `toucheous’ …while we agree with the human rights provisions and all that as a society, we must also revert to our own society. Some of the very critical questions that I would want to ask is whether or not at the society (level) we are ready for that kind of thing,” she remarked.
“We cannot, at (any) time, ignore the religious groups in our society. If we look at statistics going back to 1992 from a survey done by the Bureau of Statistics, we would see that a small section of the Guyanese population might be considered as people who do not subscribe to a religious view. While the laws are not necessarily based on moral values, we must acknowledge the fact that we might want to revert to many of the cases that (concern) laws that protect public morality.”
Nestor told the speakers at the consultation these were some of the issues that they needed to deal with.
She highlighted, too, that what must be examined is the effects of same-sex marriages on society and how this issue must be tackled.
“These are some of the things we should consider and I don’t think you dealt with that in the presentations,” Nestor stated, adding that the issues must be addressed “frontally.”
“Do we think that with the coming to being of this Bill that there might be quite a number of challenges to the Constitution in relation to the same issue of a man marrying a man? What do we do at the society (level)? Do we recognise that?” were the questions directed at the five speakers at the session.
Nestor continued: “There is some argument that says, `Oh the Bill does not promote homosexuality or does not encourage a man to marry (another) man, but if you look at Section (15) that talks about `non-discrimination’ then how can we not, with the passage of this Bill, allow a man not to marry (another) man.”
According to Nestor, matters of this nature constitute some of the “inconsistencies” of the Bill.
On the argument raised by presenters at the session on who determines what is morality, Nestor said in the concept of democracy it is the people who are the determinants based on a “line of thinking.”
She also rebutted on grounds that put the spotlight on teachers who may have been caught “interfering” with their young students and who may subsequently be dismissed from their jobs.
“Could you imagine such a person interfering with a boy below age 10 - and by virtue of the fact that the Bill is there, the judges (in the case) will have to use their discretion in terms of what happens. We will have more Constitutional changes and problems (arising with the passage of the Bill).”
SASOD member and law student, Joel Simpson, in reply, said he doesn’t think any homosexual in Guyana wants to “run into a church and ask that people marry them or anything of that sort.”
At one point likening the church to a “club”, Simpson claimed the church has the right to exclude whomever it wants. He further stated that in accordance with the Constitution, people of the same sex do not currently have the right to marry, and will also not be able to do such an act with the passage of the Bill.
However, Simpson said it is possible that the law, with the passage of the Bill, would have to recognise same-sex domestic partnerships in relation to employment benefits, sharing of properties, etc.
Simpson said he believes there should be a realm of “public” and “private” morality between consenting adults, and implied that the Bill did not fully give “rights” to homosexuals.
But, Nestor interjected: “I am informing you further…that the Sexual Orientation provision has, in fact, in some way recognised the rights of homosexuals and we must accept that.”
Meanwhile, there was no vocal Christian representative(s) at the consultation and apart from the two Opposition representatives none other participant gave comments or directed questions at the presenters.
Other speakers in support of the sexual orientation clause were: Moulana Mohammed Ali Zenjiban, Assistant Director of the International Islamic College; Denuka Radzik from Red Thread, Keimo Benjamin, UG law student and Vidyartha Kissoon from Help & Shelter.
The Sunday Chronicle has been reliably informed that the Georgetown Ministers Fellowship, representing groups of Christian leaders staunchly against sexual orientation as a right in Guyana, have recently prepared a detailed 16-page document outlining issues arising from research to further boost their argument against the inclusion of the clause.
The document is yet to be made public.
http://www.landofsixpeoples.com/news301/nc306083.htm Droits : All rights reserved by the source institution. http://ufdc.ufl.edu/AA00014700/00001 | Partager |
A Negro festival drawn from nature in the island of St. Vincent / from an original picture by Agostine Brunyas, in the possession of Sir William Young Bart. F. R. S. Auteur(s) : Brunias, Agostino Éditeur(s) : John Stockdale John Stockdale ( London ) Résumé : In 1764 Italian artist Agostino Brunias visited the West Indies with Sir William Young, as his personal artist. Brunias returned to England about 1775, and emigrated to Dominica in 1784. Edwards defended slavery in his History, the second and later editions of which are illustrated with several pictures by Brunias. This print, however, probably depicts free men and women (they are better dressed than slaves). Brunias visions of noble savages and happy slaves are often reproduced, to this day. Illustration from The History, Civil and Commercial of the British Colonies in the West Indies / Bryan Edwards. St. Vincent and the Grenadines Droits : All rights reserved by the source institution. 1995-202-1 | Partager |
Trinidad and Tobago revised ordinances, 1950 ; Laws, etc. (Trinidad and Tobago revised ordinances, 1950 Auteur(s) : Trinidad Maingot, Elliot Francis, 1891- Éditeur(s) : Printed by C. F. Roworth, Govt. printers Printed by C. F. Roworth, Govt. printers ( London ) Résumé : (Statement of Responsibility) Prepared under the authority of the Law revision ordinance, ch. 1, no. 1, by Elliot Francis Maingot. Cover title: Laws of Trinidad and Tobago, 1950. "Contains the ordinances of the colony in force on the 31st day of December, 1950, exclusive of those reserved by ordinance no.23 of 1949 and by subsequent proclamations." Trinidad and Tobago 000255255 28706053 ABC5972 | Partager |
The history of Sandford and Merton ; Sandford and Merton Auteur(s) : Day, Thomas, 1748-1789 Hazard, Willis P ( Willis Pope ), 1825-1913 ( Publisher ) Duval, Peter S., 1804 or 5-1886 ( Lithographer ) Éditeur(s) : Willis P. Hazard Willis P. Hazard ( Philadelphia ) Résumé : (Statement of Responsibility) by Thomas Day ; beautifully illustrated. Illustrations chromolithographed by P.S. Duval. Cf. Osborne Coll., p. 243-244; 874-875. Cf. Gumuchian, 2064-2088. Cf. Welch, D.A. Amer. children's books, 269. (Funding) Brittle Books Program United States -- Pennsylvania -- Philadelphia Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide. 002248792 02125712 ALK0517 | Partager Voir aussi Boys -- Conduct of life -- Juvenile fiction ( lcsh ) Conduct of life -- Juvenile fiction ( lcsh ) Friendship -- Juvenile fiction ( lcsh ) Prejudices -- Juvenile fiction ( lcsh ) Natural history -- Juvenile fiction ( lcsh ) Country life -- Juvenile fiction ( lcsh ) Courage -- Juvenile fiction ( lcsh ) Boys -- Education -- Juvenile fiction ( lcsh ) Dialogues -- 1857 ( rbgenr ) Gold stamped cloth (Binding) -- 1857 ( local ) |
True stories of American history for our young people ... Auteur(s) : Bright, Marshal Huntington, 1834-1907 Monroe Book Company ( Publisher ) Éditeur(s) : Monroe Book Company Monroe Book Company ( Chicago Ill ) Résumé : (Statement of Responsibility) by Col. Marshal H. Bright and others ; embellished with about one hundred and fifty fine engravings by the best American artists. Date of publication on t.p. verso. Title page printed in red and black. Some plates printed in green or colors. "A book for home and fireside containing thoroughly truthful narratives of the greatest, most interesting and romantic incidents from the earliest days to the present time told in easy, familiar language." United States -- Illinois -- Chicago United States United States United States Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide. 002222878 ALG3124 263147925 | Partager |
Old Spanish cannon at Cabanas, with Havana harbor in the distance ; Manuel R. Bustamante Photograph Collection. Résumé : Written on verso: 'Old Spanish cannon at Cabannas sic, with Havana harbor in the distance. Each of the old guns bears the date of its making and is designated by a name and other inscriptions and ornamentation. Cabannas sic and Morro? are little more than 'show' places now and during the tourist season are thronged with sight-seers every pleasant day. Our view shows a party of them in the distance, listening to the Military Band playing in the shade of the great laurel trees.' Also: 'Used Sept 1926.' Probably used by the Munson Steamship Line in their publication, The Cuba Review. 1920-1930 CHC5017 Havana (Cuba) Droits : http://merrick.library.miami.edu/digitalprojects/copyright.html chc50170001840001001 1439 | Partager |