Éditeur(s) : ESPE de Martinique : Ecoles Supérieures du Professorat et de l'Education ESPE de Martinique : Ecoles Supérieures du Professorat et de l'Education
Extrait de : "Lutte contre les discriminations dans l'éducation" : journée d'étude, le 4 juin 2015. Université des Antilles et de la Guyane
Description : Cette communication présente les résultats d'une expérience de terrain qui teste l'effet des représentations cognitives des relations inter-groupales des enfants sur l'interprétation des situations sociales et sur la qualité des rapports de type minorité-majorité sociale. Des groupes d'enfants portugais Blancs et Noirs, âgés de 8 à 10 ans ont pris part à la recherche. Les enfants ont participé à des situations intergroupales qui accentuaient des représentations de type « un seul groupe » (le groupe national), de type « identité double » (groupe national comprenant deux sous-groupes) ou encore « deux groupes entièrement distincts » (Blanc vs. Noir). Les deux premières représentations ont amélioré les relations entre les groupes, pour les deux, le groupe majoritaire et le groupe minoritaire. Néanmoins, pour le groupe majoritaire, la représentation « un seul groupe », mais non pas celle de l'identité double, a amélioré les intentions comportementales. L'inverse était vrai pour le groupe minoritaire mais via la représentation d'identité double. Les implications éducatives de cette recherche seront discutées.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15186
V15186
V15186

Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : The importance of longevity as an economically trait gives a picture of the flock efficiency and adaptation in a particular environment. A study was conducted in the experimental herd of Creole goats at INRA in Guadeloupe in order to test environmental (year and season at first kidding, age at first kidding and weight at first mating as well as genetic (index of resistance, sire) factors that affect longevity of does. Lifetime data set of 387 Creole does, reared at pasture all year long, was recorded over a period of 11 years (2001-2012). Does were bred for reproduction at 11months of age. Three mating periods were organized per year, corresponding to 3 climatic seasons, using buck effect. Data were analyzed using survival models (Survival Kit 6.1). The average age for culling was 5.03 years. The culling rate was higher for goats between 2 and 3 years (17 and 24%) and then gradually decreased. Year and season at first kidding did not have a long term influence on does? longevity; neither does age at first kidding. In contrast, weight at first mating had a significant effect and it can be recommended to farmers to mate primiparous goats heavier than 17 kg. Heritability was estimated to 0.16 allowing some genetic progress. No significant correlation was shown with the genetic breeding value of resistance to gastrointestinal parasitism. This study gave indications to breeders to improve their female flock management and increase does? longevity. L'importance de la longévité comme trait économique donne une image de l'efficacité et de l'adaptation de troupeau dans un environnement particulier. Une étude a été entreprise dans le troupeau expérimental de chèvres créoles à l'AICN en Guadeloupe afin d'examiner ambiant (l'année et la saison d'abord badiner, âge d'abord badiner et poids d'abord joindre aussi bien que (index de résistance, de père) les facteurs génétiques affectez dont la longévité fait. L'ensemble de données de vie du Créole 387 fait, élevé au pâturage tout au long de l'année, a été enregistré pendant 11 ans (2001-2012). Fait ont été multipliés pour la reproduction à 11months d'âge. Trois périodes d?accouplement ont été organisées par an, correspondant à 3 saisons climatiques, utilisant l'effet de mâle. Des données ont été analysées utilisant des modèles de survie (trousse de survie 6,1). L'âge moyen pour cueillir était de 5,03 ans. Le taux de cueillage était plus haut pour des chèvres entre 2 et 3 ans (17 et 24%) et alors graduellement diminué. L'année et la saison à premier badiner n'ont pas eu une influence à long terme sur la longévité des does ; ni l'un ni l'autre ne vieillit d'abord badiner. En revanche, le poids au premier accouplement a eu un effet significatif et il peut recommander aux agriculteurs de joindre les chèvres primipares des que 17 kilogrammes plus lourds. L'héritabilité a été estimée à 0,16 permettant du progrès génétique. Aucune corrélation significative n'a été montrée avec la valeur d'élevage génétique de la résistance au parasitisme gastro-intestinal. Cette étude a donné des indications aux éleveurs afin d'améliorer la gestion féminine de troupeau et d'augmenter la longévité de ces dernières.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16262
V16262
V16262

Éditeur(s) : EUTIC : le réseau international et interdisciplinaire pour les Enjeux et Usages des Technologies de l'Information et de la Communication
Extrait de : "Les écosystèmes numériques et la démocratisation informationnelle" : colloque, les 3 et 4 novembre 2015. Université des Antilles
Description : Cette communication, proposé par Nicolas Lamic, Maître de Conférences en Sciences de l'Education à l'Université des Antilles, a pour objet la présentation d'une démarche d'évaluation empirique (Tricot et al, 2003) d'un test d'implantation de compteurs électriques communicants auprès d'un échantillon de foyers martiniquais sélectionnés dans le cadre d'une recherche expérimentale menée dans l'optique d'un déploiement ultérieur de cette nouvelle technologie sur l'ensemble du territoire de la Martinique. La recherche, toujours en cours, s'organise sur deux phases. La première correspond à l'étape concomitante à l'installation du compteur au domicile des personnes sélectionnées pour l'expérimentation. Elle vise à étudier les conditions d'acceptabilité du compteur communicant. La deuxième phase quant à elle s'intéresse à l'acceptation du compteur et du portail sur lequel les consommateurs peuvent visionner leurs consommations, suivre des objectifs et recevoir des conseils.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15414
V15414

Éditeur(s) : CARDI : Caribbean Agricultural Rechearch and Development Institute Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Banana and plantain (Musa spp) are important foreign exchange earners in Guyana and the Windward Islands of the Caribbean. Black Sigatoka Disease (BSD) also referred to as black leaf streak disease is one of the most destructive diseases of banana and plantain and is present within the region. In Guyana within two years following its introduction in 2008, the industry was totally decimated, recording a 100% decline, turning the country to a net banana importer. Also, from 2009 ? 2012 the disease spread in the Windward Islands causing as much as 100% decrease in the export of banana. The disease is caused by the ascomycete fungus, Mycosphaerella fijiensis (anamorph Pseudocercospora fijiensis) and is aggressive, challenging to control and results in enormous crop damage if left uncontrolled. In an effort to develop an integrated management approach to the disease the Caribbean Agricultural Research and Development Institute (CARDI) with financial assistance from the Caribbean Development Bank (CDB) is evaluating five FHIA banana and plantain accessions and eight IITA plantain accessions for tolerance to BSD. The evaluation is presently conducted in four Caribbean countries, Dominica, Guyana, St Lucia and St Vincent and the Grenadines. In each country, four distinct agro- ecological zones were selected for the trial evaluation blocks and using a randomized block design, four blocks were established in each country. Five FHIA accessions: FHIA-01, FHIA-03, FHIA-18, FHIA-21 and FHIA-23 are being evaluated in Dominica, St Lucia and St Vincent and the Grenadines whereas, plantain accessions: PITA-17, PITA-21, PITA-22, PITA-23, PITA-24 and PITA-26 are to be evaluated in Guyana for tolerance to the disease. Cavendish are used as regional controls and as borders rows to increase disease pressure. The disease parameters evaluated are youngest leaf spotted (YLS), disease development time (DDT), leaf emission rate (LER), disease severity at 6 months, bunch emergence and harvest, and index of standing leaves. Agronomic data; days from planting to flowering, bunch weight, number of hands in bunch, average weight of hands and average number of fingers per hand and bunch, as well as organoleptic characteristics of both cooked and ripe fruits are also evaluated. The early data collection, of the FHIA only accessions, show trends in the disease tolerance and agronomic performance as well as their adaptability in the different agro-ecological zone. The trends indicate better disease tolerance of FHIA varieties with higher leaf counts before, at bunching and at harvest. The organoleptic test shows a preference to FHIA 03 and FHIA 21. La banane et le plantain (espèces de Musa) sont les acquéreurs de devises étrangères importants en Guyane et en Îles du Vent des Caraïbe. La maladie noire de Sigatoka (schéma) également désignée sous le nom de la maladie noire de filet de feuille est l'une des maladies les plus destructives de la banane et du plantain et est présente dans la région. En Guyane dans un délai de deux ans après son introduction en 2008, l'industrie a été totalement décimée, enregistrant une baisse de 100%, tournant le pays à un importateur net de banane. En outre, à partir de 2009-2012 la propagation de maladie en Îles du Vent causant pas moins de la diminution de 100% de l'exportation de la banane. La maladie est provoquée par le champignon d'ascomycète, fijiensis de Mycosphaerella (fijiensis anamorph de Pseudocercospora) et est agressive, contestant pour commander et les résultats dans l'énorme culture endommagent si laissé incontrôlé. Dans un effort de développer une approche intégrée de gestion à la maladie l'institut agricole des Caraïbes de recherche et développement (CARDI) avec l'aide financière de la banque de développement des Caraïbes (BDC) évalue des adhésions de banane et de plantain de cinq FHIA et huit adhésions de plantain d'IITA pour la tolérance au schéma. L'évaluation est actuellement effectuée quatre pays des Caraïbes, Dominique, Guyane, St Lucia et Saint-Vincent-et-les Grenadines. Dans chaque pays, quatre agro zones écologiques distinctes ont été sélectionnées pour les blocs d'essai d'évaluation et employant une conception de bloc randomisé, quatre blocs ont été établis dans chaque pays. Cinq adhésions de FHIA : FHIA-01, FHIA-03, FHIA-18, FHIA-21 et FHIA-23 sont évalués en Dominique, au St Lucia et au Saint-Vincent-et-les Grenadines tandis que, des adhésions de plantain : PITA-17, PITA-21, PITA-22, PITA-23, PITA-24 et PITA-26 doivent être évalués en Guyane pour la tolérance à la maladie. Cavendish sont employés en tant que contrôles régionaux et comme rangées de frontières pour augmenter la pression de la maladie. Les paramètres de la maladie évalués sont la plus jeune feuille repérée (YLS), le temps d'élaboration de la maladie (DDT), le taux d'émission de feuille (LER), la sévérité de la maladie à 6 mois, émergences et récoltes de groupe, et index des feuilles debout. Données agronomiques ; des jours de la plantation à la floraison, du poids de groupe, nombre de mains dans le poids de groupe et moyen des mains et du nombre moyen de doigts par main et groupe, aussi bien que les caractéristiques organoleptiques des fruits cuits et mûrs sont également évalués. La collecte de données tôt, des adhésions de FHIA seulement, exposition tend dans la tolérance de maladie et la représentation agronomique aussi bien que leur adaptabilité dans la zone agro-écologique différente. Les tendances indiquent qu'une meilleure tolérance de maladie des variétés de FHIA avec une plus haute feuille compte avant, à se rassembler et à la récolte. L'essai organoleptique montre une préférence à FHIA 03 et à FHIA 21.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16266
V16266

Éditeur(s) : Université des Antilles AREBio Groupe de recherche BIOSPHERES : BIOlogie, Sciences Physiques & Humaines pour les énergies Renouvelables, l
Extrait de : 1er colloque international BIOSPHERES, du 18 au 20 juin 2019. Université des Antilles
Description : The Chemical Purification, analysis and Screening core laboratories (CPAS) are located at the University of South Florida campus in Tampa. This core offers instrumentation and expertise in fractionation, purification, and various chemical analyses to support synthetic and natural products drug discovery research groups. Our main equipment are dedicated to chromatographic separation and purification (MPLC, HPLC) and mass-spectrometry for analytic detection, quantification and identification (LC-MS SQ, QqQ and QToF). The CPAS services also include Circular Dichroism spectrometry, general spectroscopic characterization (UV, FTIR), lyophilization (bench top and high capacity freeze-dryers), synthesis (microwave reactor), ADME screening (permeability and drug solubility testing), and high-throughput screening development capability (automated liquid handler and multimode plate reader). An example of three significant studies will be presented at the conference to illustrate the CPAS core facility services: 1) Identification of new bioactive compounds from mangrove endophytic fungi as potential alternatives to malaria drug resistance, 2) Myricanol isolated from Bayberry (Myrica Cerifera) and its synthetic derivatives targeting the microtubuleassociated protein tau accumulation in the Alzheimer's disease and 3) New ent-labdane diterpenes with solid tumor cell lines antiproliferative properties from the endemic plant Eupatorium obtusissmum from the island of Hispaniola. The core provides research lab training workshops in advanced technology and assistance so users can operate any instruments available in the laboratories to successfully accomplish their analyses and research projects.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V19063
V19063

Éditeur(s) : The American University The American University
Résumé : (Thesis) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
Six hypotheses tested that are no significant differences among faculty, students, and administrator respondent groups at the College of the Bahamas regarding importance of student growth and development, service and support goals as measured by SCGI.
Nassau, Bahamas
Bahamas

Droits : All rights reserved by the source institution.
COLOFB 1698

Éditeur(s) : University of West Indies, Barbados Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : A germination bioassay was developed to screen for potential phytotoxicity of soil amendment products from organic waste recycling. In phase 1 of the study, nine soil amendment products were applied as mulches on 3- to 5-week-old tomato seedlings (Solanum lycopersicum L.HA3019?) planted in 4L pots. Plant growth was reduced by the less mature mulch materials. Using lettuce (Lactuca sativa L.New Red Fire?) as the test species, a laboratory germination test was developed in phase 2 with aqueous extracts from a non-phytotoxic (safe) and a phytotoxic product identified in phase 1. Extracts were obtained by soaking 10g of air-dried product in 90 mL distilled water for 24h at 25°C. Diluted extracts (0, 25, 50, 75 and 100%) were applied to lettuce seeds sown on filter paper in petri-dishes and germination was monitored for 5 days, confirming the results from phase 1. In phase 3, the bioassay developed in phase 2 was used to test three previously untested local products and one imported product. Two of the local products were comparable to the imported product. Results obtained in phase 3 were confirmed using the mulch test as described in phase 1. Hence, the germination bioassay can be a useful tool for quality control in the production of(safe) soil amendment products from organic waste recycling.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16302
V16302

Résumé : Emilio Pons, declared that he is doing an excellent business. "'The required condition of the climate of Tampa for good cigars is said to be fully equal to that of Key West or Havana,' 'This has been proven by an actual and thorough test. Another advantage comes from the superior transportation facilities of the South Florida Railroad, which gets freight quickly to New York.'
(Funding) Digitized with funding from the Institute of Museum and Library Services as part of the Ephemeral Cities grant project.

Droits : All rights reserved. 2005.
C24-05722

Éditeur(s) : Physiomar 08 Physilogical aspects of reproduction, nutrition and growth "Marine molluscs in a changing environment"
Résumé : Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to B. ostreae. The first infections occurred after 4 months of challenge in the tested oysters (Family 1 and Family 2). The cumulative mortalities after 5 monts were 58% for the wil oysters, 9% for Family 1 (20-month old at the beginning of the experiment) and 20% for Family 2 (8-month, old). The parasite could be detected in 66.8% of the dying wild oysters, 67.5% of the dying oysters of Family 1, 89% of the dying oysters of Family 2 and only 11% of the surviving oysters of Family 2. The mortality was significantly higher in Family 2 thant in Family 1 (x2= 20.87, p<0.001, d.f.) as well as the level of infection by the parasite found in heart smear (x2=24.34, p<0.001, 4 d.f.). This result demonstrates that prespawning oysters as yong as 1 year-old can become infected with the parasite and die from bonamiosis. This result is inconsistent with the commonly accepted critical age of 2 years-old for the disease development. The most probable cause of the dscrepancy in the development of bonamiosis between the 2 tested families is a difference in genetic background.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2008/acte-4535.pdf
http://archimer.ifremer.fr/doc/00000/4535/

Éditeur(s) : University of Miami University of Miami ( Coral Gables, Florida )
Résumé : The purpose of this study was to investigate the level of empowerment among pregnant adolescents living in The Bahamas aged 18-19 years by testing the levels of autonomy, environmental mastery, personal growth, relationship with others, purpose in life and religiosity. The findings of the study may well be used to highlight areas for future research in pregnancy prevention programs for adolescents in The Bahamas.

Droits : All rights reserved by the source institution.

Éditeur(s) : Abeng Pub. Co. Abeng Pub. Co. ( Kingston Jamaica, Kingston, Jamaica )
Résumé : (Dates or Sequential Designation) v. 1- (no. 1- ); Feb. 1, 1969-v. 1, no. 35 (Oct. 3, 1969).
Jamaica
Jamaica
Jamaica
Jamaica

05001780
5001780

test

http://www.tramil.net/fototeca/plant15

Éditeur(s) : Elsevier
Résumé : Herbivorous (20% vegetable protein, 40% carbohydrate) and carnivorous diets (40% marine animal protein, 20% carbohydrate) were experimentally tested to assess their effect on the energy balance and energetic substrates utilized by postlarvae (PL's /15 days) and juvenile shrimp (3-6 g) of Litopenaeus vannamei. Postlarval stage 60 (PL60, early juveniles) shrimps fed HeD and CaD diets, then late juveniles (3-6 g) acclimated to the same diets were tested for their respective energy partitioning potential. No significant differences (p>0.05) on growth were obtained in early juveniles (mean final wet weight of 0.19 g). However in late juvenile stages a significant difference (p<0.05) in growth rate was observed. In terms of energy partitioning, both early and late juveniles seem to spend more energy in respiratory metabolism than in the elimination of excretion products. A change in feed composition based on quality protein sources induced some modifications on shrimp's activity measured by heat increment. Shrimps fed with an herbivorous diet showed a higher heat increment. Interestingly, the early stages of L vannamei display a remarkable capacity to assimilate a plant protein-based and a high carbohydrate level diet. Such capacity with a stimulation of genes would lead to a good adaptation of juveniles receiving all-plant diets to sustain growth performances up to a marketable size. The implications of these findings for the shrimp farming feeding costs are briefly discussed. (C) 2009 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier), 2009-11 , Vol. 296 , N. 1-2 , P. 123-128

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-7402.pdf
DOI:10.1016/j.aquaculture.2009.08.007
http://archimer.ifremer.fr/doc/00000/7402/

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Résumé : Test jpg

Droits : Ce document est protégé par le droit d'auteur. Il ne peut en aucun cas être utilisé sans l'autorisation de l'auteur et des ayant droits
Provenance : Université des Antilles et de la Guyane. Service commun de la documentation
Permalien : http://www.manioc.org/recherch/HASH9a0689272556acb40bf13b
HASH9a0689272556acb40bf13b

Éditeur(s) : HAL CCSD ACSM
Résumé : International audience
PURPOSE AND METHODS: This study was designed to compare the physiological responses and performance of well trained cyclists riding with two different chainring designs, round or eccentric, during a brief and intense cycling exercise: an "all-out" 1-km laboratory test. The eccentrically designed chainring was made of two crank arms sliding into each other, with the inside arm fixed on the center of the arm of a circular chainring and the outside arm sliding along the inside and revolving around an elliptical cam. This design increases crank arm length at the downstroke and decreases it during the upstroke, thus increasing and decreasing the torque. In terms of the chainring's revolution, the crank arm length at 0 degrees and 180 degrees is similar to the arm length of circular chainrings (175 mm). However, during the downstroke (0-180 degrees ), it increases to its maximum length of 200 mm at 90 degrees and then returns to its original length of 175 mm at 180 degrees. During the upstroke, it decreases to a minimum length of 150 mm at 270 degrees and then increases to 175 mm at 360 degrees. Eleven cyclists performed an all-out 1-km laboratory test using each chainring. The study was conducted over two consecutive weeks with the order of chainring use randomized. During all trials, ventilatory data were collected every minute using an automated breath-by-breath system. Heart rate was measured using a telemetry system. RESULTS: None of the cardiorespiratory variables showed significant differences between chainring trials. Performance, however, was significantly improved using the eccentric design (64.25 +/- 1.05 vs 69.08 +/- 1.38 s, P < 0.004, with the eccentric and the round design, respectively). CONCLUSION: We concluded that the eccentric chainring significantly improved the cycling performance during an all-out 1-km test. Further testing with indoor cycling specialists performing on a velodrome would be helpful to define the maximal possibilities of such a chainring.
Medicine & Science in Sports & exercise

hal-00720551
https://hal.univ-antilles.fr/hal-00720551
https://hal.univ-antilles.fr/hal-00720551/document
https://hal.univ-antilles.fr/hal-00720551/file/Article.pdf

Éditeur(s) : University of Florida Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Under tropical and sub-tropical climates, a sustainable development of agriculture is challenging because of the pest pressure with favorable weather conditions. Implementation of appropriate cropping systems including growing cover crops and crop rotation plays a critical role, which can break up the food chain and life cycles of field pests. Some field trials have demonstrated that among all tested summer cover crops, sunn hemp (Crotalaria juncea L. cv. Tropic sun) can grow vigorously during the rainy summer, cover the land quickly and densely, and it can produce a large quantity of biomass in two to three months. More importantly, sunn hemp can suppress soil root-knot nematodes via releasing some nematicides through its roots and through the decomposition of plant residues. Therefore, growing cover crop â? ? sunn hemp during the crop off season and rotating with valuable vegetable crops in the growing season has become a promising cropping system under the subtropical climate. Such a result may have a potential to be implemented in Caribbean countries to improve the sustainable development of agriculture.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16313
V16313

Résumé : Test jpg

Droits : Ce document est protégé par le droit d'auteur. Il ne peut en aucun cas être utilisé sans l'autorisation de l'auteur et des ayant droits
recherch:HASH9a0689272556acb40bf13b

Éditeur(s) : Inter-research
Résumé : Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20 degrees C) and not at higher temperature (30 degrees C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria.
Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2000-03 , Vol. 40 , N. 2 , P. 101-107

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00000/10543/7600.pdf
DOI:10.3354/dao040101
http://archimer.ifremer.fr/doc/00000/10543/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Are bisphenol A (BPA) and BPA analogs (BPA-A) safe for male human reproductive function? The endocrine function of human testes explants [assessed by measuring testosterone and insulin-like factor 3 (INSL3)] was impacted by exposure of the human adult testis explants to BPA/BPA-A. The few epidemiologic studies performed suggest that bisphenols have potential endocrine disruptive properties, but they did not identify clear and direct patterns of endocrine disruption. Adult human testis explants in culture were exposed to BPA and the analogs bisphenol F (BPF), bisphenol S (BPS), bisphenol E (BPE), bisphenol B (BPB) and bisphenol A diglycidyl ether (BADGE) at 10(-9)-10(-5) M for 24 or 48 h. Human adult testes were obtained from prostate cancer patients who had no hormone therapy, or from multiorgan donors. After ex vivo exposure to the investigated bisphenols, the measured outcomes were related to histopathology (gross morphology and germ cell viability determined by anti-caspase three immunohistochemistry), and the levels of testosterone, INSL3 and inhibin B were measured using immunoassays. The levels of mRNA encoding key enzymes of bisphenol biotransformation were investigated by quantitative PCR: UGT2B15 UDP (glucuronosyltransferase two family, polypeptide B15), GUSB (glucuronidase beta), SULT1A1 and 3 (sulfotransferase family 1 A member 1 and 3) and STS (steroid sulfatase). A significant dose-dependent inhibition was found between testosterone levels measured in the culture medium and concentrations of BPA (P = 0.00778 at 24 h and P = 0.0291 at 48 h), BPE (P = 0.039) and BPF (P = 0.00663). The observed BPA and BPA-A-induced inhibition of testosterone production varied according to duration of exposure and BPA/BPA-A concentrations. BPA (10(-9) M; P < 0.05), BPB (10(-9) M; P < 0.05), BPS (10(-9) and 10(-8) M; P < 0.05) and BADGE (10(-5) M; P < 0.05) increased Leydig cell INSL3 production. By contrast, BPE dose dependently inhibited INSL3 (P = 0.0372). Conversely, Sertoli cell function (inhibin B) and germ cell viability were not significantly affected by either bisphenols. N/A. Environmental compounds cannot be deliberately administered to men, justifying the use of an ex vivo approach. A relatively low number of testes samples were available for analysis (n = 3, except for testosterone secretion with n = 5). The active concentrations of BPA and BPA-A used in the study were higher than those found in human biological fluids. Under our experimental conditions, direct exposure to BPA or BPA-A can result in endocrine disturbance in the adult human testis.
ISSN: 0268-1161

hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/file/Parallel%20assessment%20of%20the%20effects%20of%20bisphenol%20A_accepted.pdf
DOI : 10.1093/humrep/dex093