Manioc : Portail | resultats 41 - 60 pour la recherche: Public Hospital Authority

Six months in the West Indies
Auteur(s) : Coleridge, Henry Nelson (1798-1843)
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Memoria sobre la esclavitud en la isla de Cuba con observaciones sobre los asertos de la prensa inglesa relativos al trafico de esclavos = Slavery in the island of Cuba, with remarks on the statements of the British press relative ot the slave trade
Auteur(s) : Torrente, Mariano (1792-1857)
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The English in the West Indies or the bow of Ulysses
Auteur(s) : Froude, James Anthony (1818-1894) Pearson, G.
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The knockabout club in the Antilles and thereabouts
Auteur(s) : Ober, Frederick Albion (1849-1913)
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Escapes from Cayenne
Auteur(s) : Chautard, Leon
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Claims on Hayti : message from the President of the United States
Auteur(s) : Committee on Foreign Affairs (United States House of Representatives)
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Antigua and the Antiguans: a full account of the colony and its inhabitants from the time of the Caribs to the present day, interspersed with anecdotes and legends : also, an impartial view of slavery and the free labour systems, the statistics of the island, and biographical notices of the principal families, Volume 2
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Identification of antigenic proteins from lichtheimia corymbifera for farmer's lung disease diagnosis
Auteur(s) : Rognon, Bénédicte Barrera, C. Monod, M. Valot, B. Roussel, S. Quadroni, M. Jouneau, S. Court-Fortune, I.
Auteurs secondaires : Laboratoire Chrono-environnement (LCE) ; Université Bourgogne Franche-Comté (UBFC) - Université de Franche-Comté (UFC) - Centre National de la Recherche Scientifique (CNRS) Institut de biologie et chimie des protéines [Lyon] (IBCP) ; Université Claude Bernard Lyon 1 (UCBL) - Centre National de la Recherche Scientifique (CNRS) Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) CHU Pontchaillou [Rennes] Service de Pneumologie [Saint-Etienne] ; CHU Saint-Etienne Service de Pneumologie et Allergologie ; CHU Clermont-Ferrand

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis. © 2016 Rognon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203

hal-01367148
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367148
DOI : 10.1371/journal.pone.0160888

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