Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We analyse temporal variations of gravity measured in southern Taiwan since November 2006 at 10 sites using absolute gravimeters and, since November 2008, at 70 sites using a relative gravimeter. We describe and apply methods to interpret the gravity changes in terms of local hydrological processes and vertical ground motions. The effect of land water is computed from local rainfall data and a model of rain accumulation and discharge in the ground. The effect of the vertical motions of the ground is estimated using time-series of permanent Global Positioning System (GPS) stations and the theoretical gravity to height ratio of −2 μGal cm−1. Unexpectedly, Morakot typhoon (2009 August), the strongest typhoon in Taiwan in 50 yr, was responsible for the highest gravity changes. Morakot triggered numerous large landslides and debris flow deposits. Their thicknesses are estimated from the gravity changes (up to ∼280 μGal) along with field observations and satellite images.
ISSN: 0956-540X

hal-00768216
https://hal.archives-ouvertes.fr/hal-00768216
DOI : 10.1093/gji/ggs019

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The island of Taiwan is located on the convergent boundary between the Philippine Sea plate and the Chinese continental margin. It offers very active mountain building and collapsing processes well illustrated by the rugged topography, rapid uplift and denudation, young tectonic landforms, active faulting and numerous earthquakes. In this paper, using simple models, we have estimated vertical movements and associated absolute gravity variations which can be expected along a profile crossing the southern part of the island and probably suffering the highest rates of rising. The two different tectonic styles proposed for the island, thin-skinned and thick-skinned, were taken into account. Horizontal and vertical movements were modeled by an elastic deformation code. Gravity variations due to these deformations are then modeled at a second step. They are dominated by plate and free-air effects, i.e. elevation of the topography, with several mu Gal yr(-1). By comparison, gravity changes generated by mass transfers are weak: maximum 0.1 mu Gal yr(-1) with the thin-skinned tectonic and 0.3 mu Gal yr(-1) with the thick-skinned tectonic. Though elastic rheology has limitations, this modeling offers interesting results on what gravity signal can be expected from the AGTO project (Absolute Gravity in the Taiwanese Orogen), which proposes to study the dynamic of these mountain ranges using absolute gravimetry (AG) and also including relative gravimetry (RG) and GPS measurements.
ISSN: 0264-3707

hal-00456035
https://hal.archives-ouvertes.fr/hal-00456035
DOI : 10.1016/j.jog.2009.09.004