Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We analyse temporal variations of gravity measured in southern Taiwan since November 2006 at 10 sites using absolute gravimeters and, since November 2008, at 70 sites using a relative gravimeter. We describe and apply methods to interpret the gravity changes in terms of local hydrological processes and vertical ground motions. The effect of land water is computed from local rainfall data and a model of rain accumulation and discharge in the ground. The effect of the vertical motions of the ground is estimated using time-series of permanent Global Positioning System (GPS) stations and the theoretical gravity to height ratio of −2 μGal cm−1. Unexpectedly, Morakot typhoon (2009 August), the strongest typhoon in Taiwan in 50 yr, was responsible for the highest gravity changes. Morakot triggered numerous large landslides and debris flow deposits. Their thicknesses are estimated from the gravity changes (up to ∼280 μGal) along with field observations and satellite images.
ISSN: 0956-540X

hal-00768216
https://hal.archives-ouvertes.fr/hal-00768216
DOI : 10.1093/gji/ggs019

Éditeur(s) : HAL CCSD
Résumé : International audience
After the 2009 Morakot typhoon, which triggered numerous large landslides in Taiwan, Mouyen et al. (2013)showed for the first time the potential of time-lapse gravity survey to infer the mass of sediments transferringby landsliding or through rivers. By providing an integrated measurement of masses, gravimetry might thus becomplementary to common methods used to assess the sediments discharge of rivers. But the masses of rocksdisplaced by Morakot were exceptionally large as a result of the record-breaking rainfalls brought by this typhoonand one might wonder to what extent time-lapse gravimetry could record such sediment transfers. In order tobetter assess the capabilities of this method, we set a time-lapse gravity network dedicated to the monitoring ofsuch sediments transfers in Paolai village (south-central Taiwan). Paolai is located near the large Laonong riverwhere temporary alluvial deposits of sediments exist and face steep mountain slopes likely to experience landslides.Both features are considered as potential source of mass transfers, and in turn of temporal gravity changes. Thefirst base gravity measurements were done in November 2015, using absolute and relative gravimeters, and will berepeated every year, before and after the typhoon season. In the same time, we also use a terrestrial lidar to scan thegeometry of both the river and the mountain slopes, hence providing a detailed topographical survey of the studiedarea. Adding Lidar measurements is an efficient strategy to solve for the non-uniqueness of gravity solutions.Meanwhile, we use the Eros morphodynamic model, that combine landsliding and flooding models, to investigatevarious scenarios of landsliding and subsequent sediment transport and compute the gravity changes on a virtualnetwork of gravimeters. This gives us insights on the expected order of magnitudes for these surface sedimenttransfers, which are useful to unravel the induced gravity signal from others sources such as local hydrology orvertical ground displacements. The modeling results also enable us to set network location that are best defined todetect mass changes associated with sediment transfer. The main objective of this study is to test whether timelapsegravity brings a new and reliable information compared to present methods monitoring sediments transfersand landscape evolution.
European Geosciences Union General Assembly 2016
Vienne, Austria

insu-01308554
https://hal-insu.archives-ouvertes.fr/insu-01308554