Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : In the new context of the agro-ecological transition, present agricultural systems will have to produce more and better in a more constraint world. Mixed crop livestock systems (MCLS) represent sound alternative ways to progressively achieve these goals through crop-livestock integration (CLI). CLI exploits the synergies between cropping and livestock systems through organic fertilization with manure or plant association, use of crop residues to feed livestock, .... It offers many opportunities to improve productivity, as well as to increase resource use efficiency and improve the resilience of the whole farming system. In the scientific literature, authors advocate the interest of MLCS and CLI, based on theoretical considerations, modelling and empirical evidence from local case studies. But these studies do not clearly identify the respective roles of diversity of activities and CLI management practices in improving performances at the level of the whole farming system. Our aim was thus to assess CLI at farm scale in a range of MCLS and to explain farm performances by analyzing the combination of activities and the level of integration. We conducted our analyses in Guadeloupe, (French West Indies), where MCLS and CLI are complex but important challenges for local agricultural. In order to analyze CLI in a holistic and systemic way, we applied a method traditionally used in ecology, ecological network analysis, to study the structure, functioning and performance of agrosystems. This method was implemented on a range of Guadeloupian MCLS where CLI practices where identified. Nitrogen was retained to conduct the analysis due to its central role for both animal and vegetal productions. The ENA method allows an estimation of productivity, resilience, efficiency, productivity and self- sufficiency of the flows network. Our results show that 1/ efficiency and resilience appear correlated to the complexity and intensity of the network of flows; 2/ efficiency and resiliency are positively correlated when we consider them in terms of N flows; 3/ in the Guadeloupian context, CLI concerns mainly specific practices, as feeding pigs with a wide range of crop residues and organic fertilization of small market gardens and plots used to grow tubers. But at whole system level, CLI remains rare and mainly depends on conventional management practices as mineral fertilization and animal complementation of some farming activities as sugar cane, cattle breeding and banana. Moreover, the combination of productions implemented determines the potential of CLI and related performances linked to relative N efficiency of each production. Consequently, performance and especially efficiency and self-sufficiency depend more on the nature of the activity than on CLI management practices. There is still a gap between theoretical studies and CLI in practice, even though CLI is more complicated than simple flows and provides other services and functions. Dans le nouveau contexte de la transition agro-écologique, les systèmes agricoles actuels devront produire plus et les améliorer dans plus de monde de contrainte. Les systèmes mélangés de bétail de culture (MCLS) représentent des manières alternatives saines d'atteindre progressivement ces buts par l'intégration de culture-bétail (CLI). Le CLI exploite les synergies entre l'emblavage et les systèmes de bétail par la fertilisation organique avec l'association d'engrais ou d'usine, utilisation des résidus de culture d'alimenter le bétail,?. Il donne beaucoup d'occasions d'améliorer la productivité, aussi bien que d'augmenter l'efficacité d'utilisation de ressource et d'améliorer la résilience du système d'exploitation agricole entier. Dans la littérature scientifique, les auteurs préconisent l'intérêt de MLCS et de CLI, basé sur des considérations théoriques, modeler et des preuves empiriques des études de cas locales. Mais ces études n'identifient pas clairement les rôles respectifs de la diversité des activités et des pratiques de gestion de CLI en améliorant des interprétations au niveau du système d'exploitation agricole entier. Notre but était ainsi d'évaluer le CLI à l'échelle de ferme dans une gamme de MCLS et d'expliquer des interprétations de ferme en analysant la combinaison des activités et du niveau de l'intégration. Nous avons réalisé nos analyses en Guadeloupe, (des Antilles françaises), où MCLS et le CLI sont des défis complexes mais importants pour agricole local. Afin d'analyser le CLI d'une manière holistique et systémique, nous avons appliqué une méthode traditionnellement employée en écologie, analyse réseau écologique, pour étudier la structure, le fonctionnement et l'interprétation des agrosystems. Cette méthode a été appliquée sur une chaîne de Guadeloupian MCLS où des pratiques en matière de CLI où identifiée. L'azote a été maintenu pour réaliser l'analyse due à son rôle central pour les productions animales et végétales. La méthode d'ENA permet une évaluation de la productivité, de la résilience, de l'efficacité, de la productivité et de l'autosuffisance du réseau d'écoulements. Nos résultats prouvent que 1 efficacités et résiliences semblent corrélées avec la complexité et l'intensité du réseau des écoulements ; 2 efficacité et élasticité sont franchement corrélés quand nous les considérons en termes d'écoulements de N ; 3 dans le contexte de Guadeloupian, CLI concerne principalement des pratiques spécifiques, en tant que les porcs de alimentation par un large éventail de résidus de culture et de fertilisation organique de petits jardins du marché et complots employés pour cultiver des tubercules. Mais à au niveau système entier, le CLI demeure rare et dépend principalement des pratiques de gestion conventionnelles en tant que complémentation minérale de fertilisation et d'animal de quelques activités agricoles comme canne à sucre, élevage et banane. D'ailleurs, la combinaison des productions mises en application détermine le potentiel du CLI et des interprétations relatives liés à l'efficacité relative de N de chaque production. En conséquence, l'interprétation et particulièrement l'efficacité et l'autosuffisance dépendent davantage de la nature de l'activité que sur des pratiques de gestion de CLI. Il y a toujours un espace entre les études et le CLI théoriques dans la pratique, quoique le CLI soit plus compliqué que des écoulements simples et fournisse d'autres services et fonctions.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16247
V16247

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : The natural 15N abundance method for estimating symbiotic biological N2-fixation was tested on legume trees from two rain forests on contrasting soils (oxisols and spodosols) in French Guiana. When possible, the significance of N2-fixing species in the plant community was evaluated in terms of density, biomass and contribution of N2-fixation to the building up of the total nitrogen mass in the leaves. Of the two sites, the rain forest on spodosols was the less favourable for application of the [delta]15N method: the available soil nitrogen was isotopically similar to fixed-N2. Hence, the results showed that a reliable estimate of N2-fixation could not be obtained. A substantial contribution of fixed-N2 to the nitrogen nutrition of legumes was found on oxisols, with an average value of 54 % Ndfa (Nitrogen derived from the atmosphere). The contribution of the N2-fixing legumes to the biomass of the stand was estimated to be 2 t ha[minus sign]1 for the leaf biomass and 136 t ha[minus sign]1 for the total above-ground plant biomass. With 7.5 % of trees in the stand able to fix N2 (462 out of 6156), N2-fixation was estimated to be 7 kg ha[minus sign]1 y[minus sign]1. These results are the first use of the [delta]15N method to estimate nitrogen input by N2-fixing legumes to a natural rain forest. The inter-site variability observed in the [delta]15N of the non-fixing plants suggested different nitrogen-cycling processes in the two soils. The [delta]15N of the non-N2-fixing plants could be related to the soil nitrogen availability and be used as an indicator of efficient or non-efficient nitrogen-cycling rain forests. The spatial variability of the [delta]15N in the plant-available soil nitrogen pool and the nitrogen balance in tropical rain forests are discussed.
ISSN: 0266-4674

hal-01032201
https://hal.archives-ouvertes.fr/hal-01032201
DOI : 10.1017/S0266467499000644

Éditeur(s) : HAL CCSD CSIRO Publishing
Résumé :  
Leaf traits of tropical tree species display an important inter-specific diversity, as detected for instance in the large range of values of leaf mass : area ratio (LMA). They also demonstrate a large irradiance-elicited plasticity, but there is still debate whether this plasticity differs among species. To address this question, leaf traits were recorded on saplings from 12 rainforest tree species in French Guiana, grown under approximately 5, 10 and 20% relative irradiance. Fifteen structural and physiological leaf traits related to photosynthesis were measured. The irradiance-elicited plasticity was quantified using a relative distance plasticity index. A large inter-specific diversity was detected for all leaf traits. A principal component analysis opposed species with a large mass-based photosynthesis, respiration, N content and photosynthetic nitrogen use efficiency, to species with a large leaf mass : area ratio, LMA. The two pioneer species used in this study displayed the largest photosynthetic capacity (and lowest LMA) and ranked at one end of the species continuum. Relative irradiance affected almost all traits with the exception of mass-based photosynthesis.Aweak interaction was found between species and relative irradiance and the species ranking was maintained among relative irradiance treatments for the majority of the traits. A principal component analysis of the values of relative-distance plasticity index failed to reveal any consistent patterns of traits or species.Weconcluded that irradiance-elicited plasticity of leaf traits was similar among species irrespective ofLMA and successional status, despite the occurrence of a large inter-specific diversity for the investigated traits
ISSN: 1445-4408

hal-01032129
https://hal.archives-ouvertes.fr/hal-01032129
DOI : 10.1071/FP09119

Éditeur(s) : HAL CCSD Springer Verlag
Résumé :  
Genetic variation for intrinsic water use efficiency (W i) and related traits was estimated in a full-sib family of Quercus robur L. over 3 years. The genetic linkage map available for this F1 family was used to locate quantitative trait loci (QTL) for W i, as estimated by leaf carbon stable isotope composition (δ 13C) or the ratio of net CO2 assimilation rate (A) to stomatal conductance to water vapour (g w) and related leaf traits. Gas exchange measurements were used to standardize estimates of A and g w and to model the sensitivity of g w to leaf-to-air vapour pressure deficit (sgVPD). δ 13C varied by more than 3‰ among the siblings, which is equivalent to 40% variation of W i. Most of the studied traits exhibited high clonal mean repeatabilities (>50%; proportion of clonal mean variability in global variance). Repeatabilities for δ 13C, leaf mass per area (LMA) and leaf nitrogen content were higher than 70%. For δ 13C, ten QTLs were detected, one of which was detected repeatedly for all 3 years and consistently explained more than 20% of measured variance. Four genomic regions were found in which co-localizing traits linked variation in W i to variations in leaf chlorophyll and nitrogen content, LMA and sgVPD. A positive correlation using clonal means between δ 13C and A/g w, as well as a co-localisation of QTL detected for both traits, can be seen as validation of the theoretical model linking the genetic architecture of these two traits.
ISSN: 1614-2942

hal-01032066
https://hal.archives-ouvertes.fr/hal-01032066
DOI : 10.1007/s11295-007-0107-z

Éditeur(s) : Actes Symposium, Bordeaux, France, 8-14 septembre 1981, pp 389-395
Résumé : Estimations of algal growth potential (AGP) of oyster-pond waters have been made by the use of bioassays and nutrient analysis. Data obtained demonstrated that nitrogen is the nutrient Iimiting AGP and that an important part of algal biomass is supported by organic nutrients. Such an uptake ranges from 0.6 to 30.7 µg-at.I-1 nitrogen ; which represents up to six times the total amount of inorganic nitrogen taken up. U.V oxydation and subsequent chemical analysis shown DON concentrations in oyster-pond waters varied with oyster presence or absence: values recorded were 10-40 µg-at N.I-1 when oysters were not present, and up to 50-60 µg-at N.I-1 when oysters were covering the bottom of the pond. The oyster Crassostrea gigas was demonstrated to excrete 77-93 % DON, versus 10-33 % N-NH4; urea excretion often represented three folds the NH4 excretion. The capability to take up organic nutrients varies with algal species ; the diatom Navicula ostrearia appeared to be the most efficient one.
L'utilisation de tests biologiques dans l'étude de la fertilité des eaux de claires ainsi que l'analyse chimique des réserves en sels nutritifs des mêmes eaux montrent qu'une part importante de la biomasse micro-algale peut être produite dans ces bassins, par assimilation par les cellules de substances organiques dissoutes. Ainsi, pour l'azote, des estimations indirectes aboutissent à des quantités de cet élément assimilé sous forme organique variant entre 0,6 et 30,7 µg-at.I-1 selon les modes d'évaluation, les espèces et l'origine des eaux, c'est-à-dire jusqu'à six fois les quantités d'ions minéraux prélevés par les algues. Ces valeurs sont tout à fait en concordance avec les teneurs en azote organique dissous effectivement présentes dans les eaux de claires: entre 10 et 40 µg-at.I-1 et jusqu'à 50 à 60 µg-at.I-1 dans les bassins où des huîtres sont immergées. On ne peut donc exclure une relation entre ces fortes valeurs en azote organique dissous et les huîtres. D'ailleurs, il est démontré que l'huître Crassostrea gigas peut excréter 77 à 93 % de rejets azotés sous forme organique, l'ammoniaque n'en représentant que 10 à 33 % et la quantité d'urée excrétée atteignant jusqu'à trois fois celle d'ammoniaque. L'utilisation de ces formes organiques de l'azote par les algues des claires varie avec les espèces; Navicula ostrearia semble être la mieux adaptée à ce type d'assimilation.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1981/acte-2946.pdf
http://archimer.ifremer.fr/doc/00000/2946/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Diploid budding yeast undergoes rapid mitosis when it ferments glucose, and in the presence of a non-fermentable carbon source and the absence of a nitrogen source it triggers sporulation. Rich medium with acetate is a commonly used pre-sporulation medium, but our understanding of the molecular events underlying the acetate-driven transition from mitosis to meiosis is still incomplete. We identified 263 proteins for which mRNA and protein synthesis are linked or uncoupled in fermenting and respiring cells. Using motif predictions, interaction data and RNA profiling we find among them 28 likely targets for Ume6, a subunit of the conserved Rpd3/Sin3 histone deacetylase-complex regulating genes involved in metabolism, stress response and meiosis. Finally, we identify 14 genes for which both RNA and proteins are detected exclusively in respiring cells but not in fermenting cells in our sample set, including CSM4, SPR1, SPS4 and RIM4, which were thought to be meiosis-specific. Our work reveals intertwined transcriptional and post-transcriptional control mechanisms acting when a MATa/α strain responds to nutritional signals, and provides molecular clues how the carbon source primes yeast cells for entering meiosis. Our integrated genomics study provides insight into the interplay between the transcriptome and the proteome in diploid yeast cells undergoing vegetative growth in the presence of glucose (fermentation) or acetate (respiration). Furthermore, it reveals novel target genes involved in these processes for Ume6, the DNA binding subunit of the conserved histone deacetylase Rpd3 and the co-repressor Sin3. We have combined data from an RNA profiling experiment using tiling arrays that cover the entire yeast genome, and a large-scale protein detection analysis based on mass spectrometry in diploid MATa/α cells. This distinguishes our study from most others in the field-which investigate haploid yeast strains-because only diploid cells can undergo meiotic development in the simultaneous absence of a non-fermentable carbon source and nitrogen. Indeed, we report molecular clues how respiration of acetate might prime diploid cells for efficient spore formation, a phenomenon that is well known but poorly understood.
ISSN: 1874-3919

hal-01122122
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122122
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122122/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122122/file/Integrated%20RNA-%20and%20protein%20profiling%20of%20fermentation.pdf
DOI : 10.1016/j.jprot.2015.01.015

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option B
Résumé : Variability of leaf traits related to photosynthesis was assessed in seedlings from 14 tree species growing in the tropical rain forest of French Guiana. Leaf photosynthetic capacity (maximum rate of carboxylation and maximum rate of electron transport) was estimated by fitting a biochemical model of photosynthesis to response curves of net CO2 assimilation rate versus intercellular CO2 mole fraction. Leaf morphology described by leaf mass per unit leaf area (LMA), density and thickness, as well as area- and mass-based nitrogen (N) and carbon (C) concentrations, were recorded on the same leaves. Large interspecific variability was detected in photosynthetic capacity as well as in leaf structure and leaf N and C concentrations. No correlation was found between leaf thickness and density. The correlations between area- and massbased leaf N concentration and photosynthetic capacity were poor. Conversely, the species differed greatly in relativeNallocation to carboxylation and bioenergetics. Principal component analysis (PCA) revealed that, of the recorded traits, only the computed fraction of total leafNinvested in photosynthesis was tightly correlated to photosynthetic capacity.We also used PCAto test to what extent species with similar shade tolerances displayed converging leaf traits related to photosynthesis. No clear-cut ranking could be detected among the shade-tolerant groups, as confirmed by a one-way ANOVA.We conclude that the large interspecific diversity in photosynthetic capacity was mostly explained by differences in the relative allocation of N to photosynthesis and not by leafNconcentration, and that leaf traits related to photosynthetic capacity did not discriminate shade-tolerance ranking of these tropical tree species.
ISSN: 0829-318X

hal-01031522
https://hal.archives-ouvertes.fr/hal-01031522

Éditeur(s) : HAL CCSD Elsevier
Résumé :  
Microbial-based indicators, such as C and N contents or microbial functions involved in C and N cycles, are currently used to describe the status of soils in disturbed areas. Microbial functions are more accurate indicators but their measurement for studies at the ecosystem level remains problematical because of the huge spatial variability of these processes and, consequently, of the large number of soil samples which must be analyzed. Our goal was to test the capacity of near-infrared reflectance spectroscopy (NIRS) to predict respiration and denitrification but also carbon and nitrogen contents of soils submitted to various procedures of restoration. To achieve this objective, we took advantage of an experiment conducted on a reforestation system established after open-cast gold mining in French Guiana. In this experimental station, plantations of various ages and various soil textures were at our disposal. Our results showed that both plantations and soil texture had a strong impact on the recovery of soil functioning: carbon and nitrogen contents, respiration and denitrification increased with age of plantation and clay content. Calibrations were performed between spectral data and microbial-based indicators using partial least squares regression (PLS). The results showed that C and N contents were accurately predicted. Microbial functions were less precisely predicted with results more accurate on clayey soils than on sandy soils. In clayey soils, perturbed or restored soils and the year of plantation were discriminated very efficiently through principal component analyses of spectral signatures (over 80% of variance explained on the first two axes). Near-infrared spectroscopy may thus be extended to the prediction of functional soil parameters, but the capacity of this method must be strengthened by expending the databases with other soils in other contexts. The possibility of using NIRS provides many opportunities for understanding both the temporal dynamics and the spatial variability of the recovery of key microbial functions during soil restoration.
ISSN: 0929-1393

hal-01032188
https://hal.archives-ouvertes.fr/hal-01032188
DOI : 10.1016/j.apsoil.2007.07.001

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Leaf dark respiration (R-dark) represents an important component controlling the carbon balance in tropical forests. Here, we test how nitrogen (N) and phosphorus (P) affect R-dark and its relationship with photosynthesis using three widely separated tropical forests which differ in soil fertility.R-dark was measured on 431 rainforest canopy trees, from 182 species, in French Guiana, Peru and Australia. The variation in R-dark was examined in relation to leaf N and P content, leaf structure and maximum photosynthetic rates at ambient and saturating atmospheric CO2 concentration.We found that the site with the lowest fertility (French Guiana) exhibited greater rates of R-dark per unit leaf N, P and photosynthesis. The data from Australia, for which there were no phylogenetic overlaps with the samples from the South American sites, yielded the most distinct relationships of R-dark with the measured leaf traits.Our data indicate that no single universal scaling relationship accounts for variation in R-dark across this large biogeographical space. Variability between sites in the absolute rates of R-dark and the R-dark : photosynthesis ratio were driven by variations in N- and P-use efficiency, which were related to both taxonomic and environmental variability.
ISSN: 0028-646X

hal-01559483
https://hal.univ-lorraine.fr/hal-01559483
DOI : 10.1111/nph.13992