Manioc : Portail | resultats 1 - 20 pour la recherche: Valentin H.

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

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Histoire de la Guadeloupe. Tome quatrième, 1803 à 1830
Auteur(s) : Lacour, Auguste (1805-1869)
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L'Oyapoc et l'Amazone : question brésilienne et française. Tome premier
Auteur(s) : Da Silva, Joaquim Caetano (1810-1873)

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Histoire des révolutions d'Haïti. Tome premier, [1789-1792]
Auteur(s) : Saint-Amand, J.
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13.904 : roman d'un forçat
Auteur(s) : Merlet, Jean-François-Louis (1878-1942)
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En Haïti : planteurs d'autrefois, nègres d'aujourd'hui
Auteur(s) : Aubin, Eugène (1863-1931)
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Journal de ma déportation à la Guyane française (fructidor an V - Ventôse an VIII) : publié d'après les manuscrits inédits
Auteur(s) : Laffon de Ladébat, André-Daniel (1746-1829)
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Rapport sur les troubles de Saint-Domingue fait au nom de la Commission des colonies, des Comités de salut public, de législation et de marine, réunis. Tome quatrième
Auteur(s) : Garran, Jean-Philippe (1748-1816)
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Année 1942 - Résumé des travaux effectués
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Upper mantle flow beneath and around the Hangay Dome, central Mongolia
Auteur(s) : Barruol, Guilhem Deschamps, Anne Déverchère, Jacques Mordvinova, Valentina V. Munkhuu, Ulziibat Perrot, Julie Artemiev, A. Dugarmaa, T.
Auteurs secondaires : Géosciences Montpellier ; Université des Antilles et de la Guyane (UAG) - Institut national des sciences de l'Univers (INSU - CNRS) - Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS) Géoazur (GEOAZUR) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Institut national des sciences de l'Univers (INSU - CNRS) - Observatoire de la Côte d'Azur - Centre National de la Recherche Scientifique (CNRS) Université européenne de Bretagne (UEB) Domaines Océaniques (LDO) ; Centre National de la Recherche Scientifique (CNRS) - Institut d'écologie et environnement - Observatoire des Sciences de l'Univers - Université de Brest (UBO) - Institut national des sciences de l'Univers (INSU - CNRS) Institute of the Earth's crust ; Siberian Branch of the Russian Academy of Sciences (SB RAS) Research Center of Astronomy and Geophysics of the Mongolian Academy of Science

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mongolia represents the northernmost area affected by the India–Asia collision, and it is actively deformed along transpressive belts closely associated with large-scale strike-slip faults. The active and past mantle flow beneath this region is, however, poorly known. In order to investigate deep mantle deformation beneath central Mongolia and its relation with the surrounding major structures such as the Siberian craton, the Gobi–Altay belt and the Baikal rift, a NS-trending profile of broadband seismic stations has been deployed in the summer 2003 from the southern Siberian craton to the Gobi–Altay range, crossing the entire Hangay dome. Mantle flow is deduced from the splitting of teleseismic shear waves such as SKS phases. In eastern Mongolia, the permanent station ULN in Ulaanbaatar reveals the presence of two anisotropic layers, the upper one being oriented NE–SW, close to the trend of lithospheric structures and the lower one NW–SE, close to the trend of Eurasia absolute plate motion. Along the NS profile in central Mongolia, seismic anisotropy deduced from SKS splitting reveals a homogeneous NW–SE trending structure, fully consistent with the observations made in the Altay–Sayan in western Mongolia. The observed delay times of 1.5 to more than 2.0 s favor consistent mantle flow over large mantle thicknesses. Since the lithosphere is less than 100 km thick beneath central Mongolia and since the observed fast directions are parallel to the trend of the lithospheric structures but also close to the trend of the absolute plate motion, we propose that both the lithosphere and the asthenosphere may join their anisotropic effects beneath central Mongolia to explain the large delay times. Although GPS vectors represent the instantaneous displacement of the Earth's surface and SKS splitting the time and vertical integration of finite strain at depth, we use the opportunity of the dense geodetic measurements available in this region to discuss the anisotropy pattern in term of present-day deformation. In the Eurasia-fixed reference frame, GPS and SKS both depict a similar trend beneath central Mongolia, suggesting a lithospheric block “escaping” toward the east that could orient olivine a-axes in the upper mantle, within a transpressive tectonic regime. A different behaviour is observed in western Mongolia: the GPS vectors trend NS, close to the regional compression direction, whereas the fast SKS directions trend EW, suggesting a tectonic regime close to a mode of axial shortening, generating the development of an EW-trending foliation at depth. We therefore propose that Mongolia is a place where active and frozen lithospheric deformation may add their effects, together with the sublithospheric flow. In the three sources of anisotropy inferred, a fundamental role is played by the Siberian craton that acted as an undeformable core of the continent through time: the frozen Paleozoic and Mesozoic structures wrap around the craton, building up the fast anisotropic direction pattern; the present-day sublithospheric flow induced by the plate motion is likely deflecting around its deep roots; finally, the present-day tectonic regime appears to be controlled by the presence of the craton to the north.
ISSN: 0012-821X

hal-00407853
https://hal.archives-ouvertes.fr/hal-00407853
https://hal.archives-ouvertes.fr/hal-00407853/document
https://hal.archives-ouvertes.fr/hal-00407853/file/Barruol_SKS_Mongolia_EPSL2008_hal.pdf
DOI : 10.1016/j.epsl.2008.07.027

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Catalogue des produits des colonies françaises envoyés à l'exposition universelle de Londres de 1862
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