Éditeur(s) : HAL CCSD
Résumé : National audience
Congrès de la SPTC - Société de Pharmaco-Toxicologie Cellulaire
Paris, France

hal-01122677
https://hal.archives-ouvertes.fr/hal-01122677

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Exposure to disturbance is rarely considered in marine protected area planning. Typically, representing and replicating the habitat types present within protected areas is used to spread the risk of protecting frequently disturbed sites. This was the approach used during the 2004 re-zoning of the Great Barrier Reef Marine Park (GBRMP) via the Representative Areas Program. Over 10 years later, we examine whether the risk was spread by mapping exposure of coral reefs in the GBRMP to four disturbances that cause coral mortality: bleaching, tropical cyclones, crown-of-thorns starfish outbreaks, and freshwater inundation. Our objectives were to: (1) assess whether no-take areas include a range of disturbance regimes, and (2) identify coral reef areas with lower relative exposure. At least 13% and an average of 31% of reef locations in each of 11 exposure classes are included within no-take areas. A greater proportion of low-exposure areas are within no-take areas than high-exposure areas (34.2% vs. 28.3%). The results demonstrate the value of risk spreading when exposure data are not available while also showing that regularly assessing exposure increases capacity for adaptive, resilience-based reef management.
ISSN: 1755-263X

Droits : http://creativecommons.org/licenses/by/
hal-01225201
https://hal-univ-perp.archives-ouvertes.fr/hal-01225201
https://hal-univ-perp.archives-ouvertes.fr/hal-01225201/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01225201/file/Maynard_et_al-2015-Conservation_Letters.pdf
DOI : 10.1111/conl.12198

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : Copernicus Gesellschaft Mbh
Résumé : During the GWADASEIS cruise (Lesser Antilles volcanic arc, February-March 2009) a very high resolution (VHR) seismic-reflection survey was performed in order to constrain Late Quaternary to Present faulting. The profiles we obtained evidence frequent "ponding" of reworked sediments in the deepest areas, similar to the deposition of Mediterranean "homogenites". These bodies are acoustically transparent (few ms t.w.t. thick) and are often deposited on the hanging walls of dominantly normal faults, at the base of scarps. Their thickness appears sufficient to compensate (i.e. bury) co-seismic scarps between successive earthquakes, resulting in a flat and horizontal sea floor through time. In a selected area (offshore Montserrat and Nevis islands), piston coring (4 to 7m long) was dedicated to a sedimentological analysis of the most recent of these particular layers. It corresponds to non-stratified homogenous calcareous silty sand (reworked calcareous plankton and minor volcanoclastics). This layer can be up to 2 m thick, and overlies fine-grained hemipelagites. The upper centimeters of the latter represent the normal RedOx water/sediment interface. Pb-210 and Cs-137 activities lack in the massive sands, while a normal profile of unsupported Pb-210 decrease is observed in the hemipelagite below, together with a Cs-137 peak corresponding to the Atmospheric Nuclear Experiments (1962). The RedOx level was thus capped by a recent instantaneous major sedimentary event considered as post-1970 AD; candidate seismic events to explain this sedimentary deposits are either the 16 March 1985 earthquake or the 8 October 1974 one (M-w = 6.3 and M-w = 7.4, respectively). This leads to consider that the syntectonic sedimentation in this area is not continuous but results from accumulation of thick homogenites deposited after the earthquakes (as observed in the following weeks after Haiti January 2010 event, McHugh et al., 2011). The existence of such deposits suggests that, in the area of study, vertical throw likely results from cumulated effects of separated earthquakes rather than from aseismic creep. Examination of VHR profiles shows that all major co-seismic offsets are recorded in the fault growth sequence and that co-seismic offsets can be precisely estimated. By using a sedimentation rate deduced from Pb-210 decrease curve (0.5 mmyr(-1)) and taking into account minor reworking events detected in cores, we show that the Redonda system may have been responsible for five > M-6 events during the last 34 000 yr. The approach presented in this work differs from fault activity analyses using displaced sets of isochronous surfaces and postulating co-seismic offsets. Combining VHR seismic imagery and coring we can decipher co-seismic vs. slow continuous displacement, and thus actually estimate the amplitude and the time distribution of major co-seismic offsets.
Natural Hazards And Earth System Sciences (1561-8633) (Copernicus Gesellschaft Mbh), 2012 , Vol. 12 , N. 5 , P. 1755-1767

Droits : Author(s) 2012. CC Attribution 3.0 License.
http://archimer.ifremer.fr/doc/00089/20040/21990.pdf
DOI:10.5194/nhess-12-1755-2012
http://archimer.ifremer.fr/doc/00089/20040/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Anthropogenic impacts are increasingly affecting the world's oceans. Networks of marine protected areas (MPAs) provide an option for increasing the ecological and economic benefits often provided by single MPAs. It is vital to empirically assess the effects of MPA networks and to prioritize the monitoring data necessary to explain those effects. We summarize the types of MPA networks based on their intended management outcomes and illustrate a framework for evaluating whether a connectivity network is providing an outcome greater than the sum of individual MPA effects. We use an analysis of an MPA network in Hawai'i to compare networked MPAs to non-networked MPAs to demonstrate results consistent with a network effect. We assert that planning processes for MPA networks should identify their intended outcomes while also employing coupled field monitoring-simulation modeling approaches, a powerful way to prioritize the most relevant monitoring data for empirically assessing MPA network performance.
ISSN: 1932-6203

Droits : http://creativecommons.org/licenses/by/
hal-01341695
https://hal-univ-perp.archives-ouvertes.fr/hal-01341695
https://hal-univ-perp.archives-ouvertes.fr/hal-01341695/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01341695/file/journal.pone.0102298.PDF
DOI : 10.1371/journal.pone.0102298

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : International audience
During the GWADASEIS cruise (Lesser Antilles volcanic arc, February-March 2009) a very high resolution (VHR) seismic-reflection survey was performed in order to constrain Late Quaternary to Present faulting. The profiles we obtained evidence frequent "ponding" of reworked sediments in the deepest areas, similar to the deposition of Mediterranean "homogenites". These bodies are acoustically transparent (few ms t.w.t. thick) and are often deposited on the hanging walls of dominantly normal faults, at the base of scarps. Their thickness appears sufficient to compensate (i.e. bury) co-seismic scarps between successive earthquakes, resulting in a flat and horizontal sea floor through time. In a selected area (offshore Montserrat and Nevis islands), piston coring (4 to 7 m long) was dedicated to a sedimentological analysis of the most recent of these particular layers. It corresponds to non-stratified homogenous calcareous silty sand (reworked calcareous plankton and minor volcanoclastics). This layer can be up to 2 m thick, and overlies finegrained hemipelagites. The upper centimeters of the latter represent the normal RedOx water/sediment interface. 210Pb and 137Cs activities lack in the massive sands, while a normal profile of unsupported 210Pb decrease is observed in the hemipelagite below, together with a 137Cs peak correspond- ing to the Atmospheric Nuclear Experiments (1962). The RedOx level was thus capped by a recent instantaneous major sedimentary event considered as post-1970 AD; candidate seismic events to explain this sedimentary deposits are either the 16 March 1985 earthquake or the 8 October 1974 one (Mw=6.3 and Mw=7.4, respectively). This leads to consider that the syntectonic sedimentation in this area is not continuous but results from accumulation of thick homogenites deposited after the earthquakes (as observed in the following weeks after Haiti January 2010 event, McHugh et al., 2011). The existence of such deposits suggests that, in the area of study, vertical throw likely results from cumulated effects of separated earthquakes rather than from aseismic creep. Examination of VHR profiles shows that all major co-seismic offsets are recorded in the fault growth sequence and that co-seismic offsets can be precisely estimated. By using a sedimentation rate deduced from 210Pb decrease curve (0.5 mm yr−1) and taking into account minor reworking events detected in cores, we show that the Redonda system may have been responsible for five >M6 events during the last 34 000 yr. The approach presented in this work differs from fault activity analyses using displaced sets of isochronous surfaces and postulating co-seismic offsets. Combining VHR seismic imagery and coring we can decipher co-seismic vs. slow continuous displacement, and thus actually estimate the amplitude and the time distribution of major co-seismic offsets.
ISSN: 1561-8633

insu-00844581
https://hal-insu.archives-ouvertes.fr/insu-00844581
https://hal-insu.archives-ouvertes.fr/insu-00844581/document
https://hal-insu.archives-ouvertes.fr/insu-00844581/file/nhess-12-1755-2012.pdf
DOI : 10.5194/nhess-12-1755-2012

Éditeur(s) : HAL CCSD Springer Verlag (Germany) Springer Verlag
Résumé : International audience
ISSN: 1292-3818

hal-01118280
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118280
DOI : 10.1007/s10269-011-1983-2

Éditeur(s) : Cambridge Univ Press
Résumé : Integrated Multi-Trophic Aquaculture takes advantage of the mutualism between some detritivorous fish and phytoplankton. The fish recycle nutrients by consuming live (and dead) algae and provide the inorganic carbon to fuel the growth of live algae. In the meanwhile, algae purify the water and generate the oxygen required by fishes. Such mechanism stabilizes the functioning of an artificially recycling ecosystem, as exemplified by combining the euryhaline tilapia Sarotherodon melanotheron heudelotii and the unicellular alga Chlorella sp. Feed addition in this ecosystem results in faster fish growth but also in an increase in phytoplankton biomass, which must be limited. In the prototype described here, the algal population control is exerted by herbivorous zooplankton growing in a separate pond connected in parallel to the fish-algae ecosystem. The zooplankton production is then consumed by tilapia, particularly by the fry and juveniles, when water is returned to the main circuit. Chlorella sp. and Brachionus plicatilis are two planktonic species that have spontaneously colonized the brackish water of the prototype, which was set-up in Senegal along the Atlantic Ocean shoreline. In our system, water was entirely recycled and only evaporation was compensated (1.5% volume/day). Sediment, which accumulated in the zooplankton pond, was the only trophic cul-de-sac. The system was temporarily destabilized following an accidental rotifer invasion in the main circuit. This caused Chlorella disappearance and replacement by opportunist algae, not consumed by Brachionus. Following the entire consumption of the Brachionus population by tilapias, Chlorella predominated again. Our artificial ecosystem combining S. m. heudelotii, Chlorella and B. plicatilis thus appeared to be resilient. This farming system was operated over one year with a fish productivity of 1.85 kg/m(2) per year during the cold season (January to April).
Animal (1751-7311) (Cambridge Univ Press), 2013-02 , Vol. 7 , N. 2 , P. 322-329

Droits : The Animal Consortium 2012
http://archimer.ifremer.fr/doc/00118/22955/20945.pdf
DOI:10.1017/S1751731112001279
http://archimer.ifremer.fr/doc/00118/22955/

Éditeur(s) : HAL CCSD Copernicus Publications on behalf of the European Geosciences Union
Résumé : International audience
During the GWADASEIS cruise (Lesser Antilles volcanic arc, February–March 2009) a very high resolution (VHR) seismic-reflection survey was performed in order to constrain Late Quaternary to Present faulting. The profiles we obtained evidence frequent " ponding " of reworked sediments in the deepest areas, similar to the deposition of Mediterranean " homogenites ". These bodies are acoustically transparent (few ms t.w.t. thick) and are often deposited on the hanging walls of dominantly normal faults, at the base of scarps. Their thickness appears sufficient to compensate (i.e. bury) co-seismic scarps between successive earthquakes, resulting in a flat and horizontal sea floor through time. In a selected area (offshore Montserrat and Nevis islands), piston coring (4 to 7 m long) was dedicated to a sedimento-logical analysis of the most recent of these particular layers. It corresponds to non-stratified homogenous calcareous silty sand (reworked calcareous plankton and minor volcanoclas-tics). This layer can be up to 2 m thick, and overlies fine-grained hemipelagites. The upper centimeters of the latter represent the normal RedOx water/sediment interface. 210 Pb and 137 Cs activities lack in the massive sands, while a normal profile of unsupported 210 Pb decrease is observed in the hemipelagite below, together with a 137 Cs peak corresponding to the Atmospheric Nuclear Experiments (1962). The RedOx level was thus capped by a recent instantaneous major sedimentary event considered as post-1970 AD; candidate seismic events to explain this sedimentary deposits are either the 16 March 1985 earthquake or the 8 October 1974 one (M w = 6.3 and M w = 7.4, respectively). This leads to consider that the syntectonic sedimentation in this area is not continuous but results from accumulation of thick homogen-ites deposited after the earthquakes (as observed in the following weeks after Haiti January 2010 event, McHugh et al., 2011). The existence of such deposits suggests that, in the area of study, vertical throw likely results from cu-mulated effects of separated earthquakes rather than from aseismic creep. Examination of VHR profiles shows that all major co-seismic offsets are recorded in the fault growth sequence and that co-seismic offsets can be precisely estimated. By using a sedimentation rate deduced from 210 Pb decrease curve (0.5 mm yr −1) and taking into account minor reworking events detected in cores, we show that the Redonda system may have been responsible for five > M 6 events during the last 34 000 yr. The approach presented in this work differs from fault activity analyses using displaced sets of isochronous surfaces and postulating co-seismic offsets. Combining VHR seismic imagery and coring we can decipher co-seismic vs. slow continuous displacement, and thus actually estimate the amplitude and the time distribution of major co-seismic offsets.
Natural Hazards and Earth System Science

insu-01585969
https://hal-insu.archives-ouvertes.fr/insu-01585969
https://hal-insu.archives-ouvertes.fr/insu-01585969/document
https://hal-insu.archives-ouvertes.fr/insu-01585969/file/nhess-12-1755-2012-beck.pdf
DOI : 10.5194/nhess-12-1755-2012

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
A new method to grow large single crystals with stoichiometry close to 1:1:2 of CuInSe2, an important member of the I-III-VI2 family of chalcopyrite semiconductors for optoelectronic device applications, is described. This is called the horizontal varying gradient zone freeze technique and consists of the selenization in the liquid phase of stoichiometric Cu and In and later solidification under programmed varying gradient in a fully automated multiple zone horizontal furnace. All ingots obtained with Se pressure between 15 and 1760 mm of Hg were single phase having chalcopyrite structure and p-type conductivity. X-ray, DTA, optical and electrical characterizations of the samples taken from different parts of the ingots were made. From energy dispersive spectroscopy it is found that the samples of the ingot obtained with Se pressure at 836 mm of Hg were much closer to the ideal stoichiometry 1:1:2.
ISSN: 0022-0248

hal-00808624
https://hal.univ-antilles.fr/hal-00808624
DOI : 10.1016/0022-0248(95)00346-0