Éditeur(s) : HAL CCSD American Society of Tropical Medicine and Hygiene
Résumé : International audience
We investigated infection of rodents and shrews by Leptospira spp. in two localities of Cambodia (Veal Renh, Kaev Seima) and in four types of habitat (forests, non-flooded lands, lowland rain-fed paddy fields, houses) during the wet and the dry seasons. Habitat preference was common, and rodent and shrew species were found only in houses or in rain-fed paddy fields or in forests. Among 649 small mammals trapped belonging to 12 rodent species and 1 shrew species, 71 of 642 animals tested were carriers of Leptospira according to the 16S ribosomal RNA marker used. Rodent infection was higher in low-slope locations, corresponding to rain-fed paddy fields, especially in the rainy season and in Kaev Seima. Rodents (Rattus exulans) and shrews (Suncus murinus) inhabiting households showed significantly low levels of infections, whereas rodents living in and near to forests (shrubby wasteland, orchards) showed high levels of infection.
American Journal of Tropical Medicine and Hygiene

pasteur-00808144
https://hal-riip.archives-ouvertes.fr/pasteur-00808144
DOI : 10.4269/ajtmh.2012.11-0349
PUBMED : 22665613

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : In the age of European exploration, terra incognita referred to lands that were largely unknown to Europeans. In the world of contemporary tourism, tourism incognita refers to the lands and experiences that are largely unknown to the global mass tourism industry (tourism cognita). Tourism incognita includes specialty niche tourisms that operate on the edge and beyond of mass tourism, as well as the entire world of non-tourism places. Examples of tourism cognita and tourism incognita that are discussed include: Dubai and Oman’s Musandam Peninsula, Cambodia’s Angkor Wat and the Tonle Sap, and the scuba diving destination of Manado on the Indonesian island of Sulawesi. Tourism incognita is shown to be vital to the success of the tourism economy because the places and experiences of tourism incognita are part of the imagination that drives the decision and desire to travel to a distant land.
À l'ère de l'exploration européenne, la terra incognita faisait référence aux terres alors largement inconnues des Européens. Dans le monde du tourisme contemporain, le tourisme incognita se renvoie aux terres et aux expériences qui sont en grande partie inconnues de l'industrie du tourisme de masse globale (tourisme cognita). Tourisme incognita comprend des créneaux touristiques spécialisés qui opèrent sur les marges du tourisme de masse, ainsi que l'ensemble du monde dela non-lieu touristique. Des exemples de tourisme et le tourisme cognita incognita traités sont: Dubaï et Oman Musandam Peninsula, Angkor wat au Cambodge et du Tonlé Sap et la destination de Manado plongée sous-marine sur l'île indonésienne de Sulawesi. Tourisme incognita est révélée déterminante pour le succès de l'économie touristique parce que les lieux et les expériences de tourisme incognita font partie de l'imaginaire qui entraîne la décision et le désir de voyager dans un pays lointain.
Cambodge
Dubaï
Indonésie
Oman

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5232
http://journals.openedition.org/etudescaribeennes/5232

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
A major requirement for malaria elimination is the development of transmission-blocking interventions. In vitro transmission-blocking bioassays currently mostly rely on the use of very few Plasmodium falciparum reference laboratory strains isolated decades ago. To fill a piece of the gap between laboratory experimental models and natural systems, the purpose of this work was to determine if culture-adapted field isolates of P. falciparum are suitable for in vitro transmission-blocking bioassays targeting functional maturity of male gametocytes: exflagellation. Plasmodium falciparum isolates were adapted to in vitro culture before being used for in vitro gametocyte production. Maturation was assessed by microscopic observation of gametocyte morphology over time of culture and the functional viability of male gametocytes was assessed by microscopic counting of exflagellating gametocytes. Suitability for in vitro exflagellation-blocking bioassays was determined using dihydroartemisinin and methylene blue. In vitro gametocyte production was achieved using two isolates from French Guiana and two isolates from Cambodia. Functional maturity of male gametocytes was assessed by exflagellation observations and all four isolates could be used in exflagellation-blocking bioassays with adequate response to methylene blue and dihydroartemisinin. This work shows that in vitro culture-adapted P. falciparum field isolates of different genetic background, from South America and Southeast Asia, can successfully be used for bioassays targeting the male gametocyte to gamete transition, exflagellation.
ISSN: 1475-2875

Droits : http://creativecommons.org/licenses/by/
pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/file/LeBas%20et%202015%20version%20publie%20%281%29.pdf
DOI : 10.1186/s12936-015-0752-x