Éditeur(s) : HAL CCSD
Résumé : International audience
Japan Geological Union Meeting
Chiba, Japan

insu-01133044
https://hal-insu.archives-ouvertes.fr/insu-01133044

Éditeur(s) : HAL CCSD Geological Society of America
Résumé : International audience
Integrated Ocean Drilling Program Expedition 313 continuously cored uppermost Eocene to Miocene sequences on the New Jersey shallow shelf (Sites M27, M28, and M29). Previously, 15 Miocene (ca. 23-13 Ma) seismic sequence boundaries were recognized on several generations of multichannel seismic profi les using criteria of onlap, downlap, erosional truncation, and toplap. We independently recognize sequence boundaries in the cores and logs based on an integrated study of core surfaces, lithostratigraphy and process sedimentology (grain size, mineralogy, facies, and paleoenvironments), facies successions, stacking patterns, benthic foraminiferal water depths, downhole logs, core gamma logs, and chronostratigraphic ages. We use a velocitydepth function to predict the depths of seismic sequence boundaries that were tested by comparison with major core surfaces, downhole and core logs, and synthetic seismograms. Using sonic velocity (core and downhole), core density, and synthetic seismograms, we show that sequence boundaries correspond with acoustic impedance contrasts, although other stratal surfaces (e.g., maximum fl ooding and transgressive surfaces) also produce refl ections. Core data are suffi cient to link seismic sequence boundaries to impedance contrasts in 9 of 12 instances at Site M27, 6 of 11 instances at Site M28, and 8 of 14 instances at Site M29. Oligocene sequences have minimal lithologic and seismic expression due to deep-water locations on clinoform bottomsets. Miocene sequences (ca. 23-13 Ma) were sampled across several unconformity clinothems (prograding units) on topset, foreset, and bottomset locations. Excellent recovery allows core-seismic integration that confi rms the hypothesis that unconformities are a primary source of impedance contrasts. Our core-seismic-log correlations predict that key seismic surfaces observed in other sub surface investigations without core and/or well logs are stratal surfaces with sequence stratigraphic signifi cance.
EISSN: 1553-040X

insu-00946235
https://hal-insu.archives-ouvertes.fr/insu-00946235
DOI : 10.1130/GES00858.1

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0887-2333

hal-00866007
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00866007
DOI : 10.1016/j.tiv.2013.07.003
PUBMED : 23850984

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Primary hepatocyte cultures are now considered as convenient models for in vitro analyzing liver drug transport. However, if primary human and rat hepatocytes have been well-characterized with respect to drug transporter expression and regulation, much less is known for primary mouse hepatocytes. The present study was therefore designed to gain insights about this point. The profile of sinusoidal and canalicular drug transporter mRNA expression in short time (4h)-cultured mouse hepatocytes was found to be highly correlated with that of freshly isolated hepatocytes; by contrast, those of counterparts cultured for a longer time (until 4days) either in monolayer configurations on plastic or collagen or in sandwich configuration with matrigel were profoundly altered: uptake drug transporters such as Oct1, Oatps and Oat2 were thus down-regulated, whereas most of efflux transporters such as Mdr1a/b, Mrp3, Mrp4 and Bcrp were induced. Moreover, short time-cultured hepatocytes exhibited the highest levels of sinusoidal influx transporter activities. Transporter-mediated drug secretion into canalicular networks was however only observed in sandwich-cultured hepatocytes. Mouse hepatocytes cultured either in monolayer or sandwich configurations were finally shown to exhibit up-regulation of referent transporters in response to exposure to prototypical activators of the drug sensing receptors pregnane X receptor, aryl hydrocarbon receptor or constitutive androstane receptor. Taken together, these data demonstrate the feasibility of using primary mouse hepatocytes for investigating potential interactions of xenobiotics with hepatic transporter activity or regulation, provided that adequate culture conditions are retained.
ISSN: 0928-0987

inserm-00823243
http://www.hal.inserm.fr/inserm-00823243
DOI : 10.1016/j.ejps.2013.01.013
PUBMED : 23396053

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
The IL-33/ST2 axis plays a protective role in T-cell-mediated hepatitis, but little is known about the functional impact of endogenous IL-33 on liver immunopathology. We used IL-33-deficient mice to investigate the functional effect of endogenous IL-33 in concanavalin A (Con A)-hepatitis. IL-33(-/-) mice displayed more severe Con A liver injury than wild-type (WT) mice, consistent with a hepatoprotective effect of IL-33. The more severe hepatic injury in IL-33(-/-) mice was associated with significantly higher levels of TNF-alpha and IL-1 beta and a larger number of NK cells infiltrating the liver. The expression of Th2 cytokines (IL-4, IL-10) and IL-17 was not significantly varied between WT and IL-33(-/-) mice following Con A-hepatitis. The percentage of CD25(+) NK cells was significantly higher in the livers of IL-33(-/-) mice than in WT mice in association with upregulated expression of CXCR3 in the liver. Regulatory T cells (Treg cells) strongly infiltrated the liver in both WT and IL-33(-/-) mice, but Con A treatment increased their membrane expression of ST2 and CD25 only in WT mice. In vitro, IL-33 had a significant survival effect, increasing the total number of splenocytes, including B cells, CD4(+) and CD8(+) T cells, and the frequency of ST2(+) Treg cells. In conclusion, IL-33 acts as a potent immune modulator protecting the liver through activation of ST2(+) Treg cells and control of NK cells.
ISSN: 0193-1857

hal-01366372
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01366372
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01366372/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01366372/file/Ablation%20of%20interaction%20between%20IL-33%20and%201%20ST2.pdf
DOI : 10.1152/ajpgi.00097.2016

Éditeur(s) : HAL CCSD
Résumé : International audience
Geophysical Research Abstracts
Vienne, Austria

insu-00609949
https://hal-insu.archives-ouvertes.fr/insu-00609949

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Purpose The purpose of this review was to examine the comprehensively accumulated data regarding potential therapeutic aspects of exogenous administration of interleukin 33 (IL-33) or its antagonists in allergic, cancerous, infectious, and inflammatory diseases. Methods A selected review was undertaken of publications that examined the protective and exacerbating effects of IL-33 or its inhibitors in different diseases. Mechanisms of action are summarized to examine the putative role of IL-33 in various diseases. Findings IL-33 promoted antibacterial, antiviral, anti-inflammatory, and vaccine adjuvant functions. However, in TH2-biased respiratory, allergic, parasitic, and inflammatory conditions, IL-33 exhibited disease-sensitizing effects. The alarmin cytokine IL-33 induced protective effects in diseases via recruitment of regulatory T cells; antiviral CD8+ cells, natural killer cells, γδ T cells, and nuocytes; antibacterial and antifungal neutrophils or macrophages; vaccine-associated B/T cells; and inhibition of nuclear factor–κB–mediated gene transcription. In contrast, IL-33 exacerbated the disease process by increasing TH2 cytokines, IgE and eosinophilic immune responses, and inhibition of leukocyte recruitment in various diseases. Implications The protective or exacerbated aspects of use of IL-33 or its inhibitors are dependent on the type of infection or inflammatory condition, duration of disease (acute or chronic), organ involved, cytokine microenvironment, dose or kinetics of IL-33, and genetic predisposition. The alarmin cytokine IL-33 acts at cellular, molecular, and transcriptional levels to mediate pluripotent functions in various diseases and has potential therapeutic value to mitigate the disease process
ISSN: 0149-2918

hal-01295637
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01295637
PUBMED : 26992663
DOI : 10.1016/j.clinthera.2016.02.021

Éditeur(s) : HAL CCSD Geological Society of America
Résumé : International audience
In this study, a novel application of a statistical approach is utilized for analysis of downhole logging data from Miocene-aged siliciclastic shelf sediments on the New Jersey Margin (eastern USA). A multivariate iterative nonhierarchical cluster analysis (INCA) of spectral gamma-ray logs from Integrated Ocean Drilling Program (IODP) Expedition 313 enables lithology within this siliciclastic succession to be inferred and, through comparison with the 1311 m of recovered core, a continuous assessment of depositional sequences is constructed. Signifi cant changes in INCA clusters corroborate most key stratigraphic surfaces interpreted from the core, and this result has particular value for surface recognition in intervals of poor core recovery. This analysis contributes to the evaluation of sequence stratigraphic models of large-scale clinoform complexes that predict depositional environments, sediment composition, and stratal geometries in response to sea-level changes. The novel approach of combining statistical analysis with detailed lithostratigraphic and seismic refl ection data sets will be of interest to any scientists working with downhole logs, especially spectral gamma-ray data, and also provides a reference for the strengths and weaknesses of multi component analysis applied to continental margin lithofacies. The method presented here is appropriate for evaluating successions elsewhere and also has value for hydrocarbon exploration where sequence stratigraphy is a fundamental tool.
EISSN: 1553-040X

insu-00858238
https://hal-insu.archives-ouvertes.fr/insu-00858238
DOI : 10.1130/GES00913.1

Éditeur(s) : HAL CCSD
Résumé : International audience
AGU Fall Meeting 2010
San Francisco, United States

insu-01133024
https://hal-insu.archives-ouvertes.fr/insu-01133024

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Human hepatocytes cultured in a monolayer configuration represent a well-established in vitro model in liver toxicology, notably used in drug transporter studies. Polarized status of drug transporters, i.e., their coordinated location at sinusoidal or canalicular membranes, remains however incompletely documented in these cultured hepatocytes. The present study was therefore designed to analyze transporter expression and location in such cells. Most of drug transporters were first shown to be present at notable mRNA levels in monolayer-cultured human hepatocytes. Cultured human hepatocytes, which morphologically exhibited bile canaliculi-like structures, were next demonstrated, through immunofluorescence staining, to express the influx transporters organic anion transporting polypeptide (OATP) 1B1, OATP2B1 and organic cation transporter (OCT) 1 and the efflux transporter multidrug resistance-associated protein (MRP) 3 at their sinusoidal pole. In addition, the efflux transporters P-glycoprotein and MRP2 were detected at the canalicular pole of monolayer-cultured human hepatocytes. Moreover, canalicular secretion of reference substrates for the efflux transporters bile salt export pump, MRP2 and P-glycoprotein as well as sinusoidal drug transporter activities were observed. This polarized and functional expression of drug transporters in monolayer-cultured human hepatocytes highlights the interest of using this human in vitro cell model in xenobiotic transport studies.
ISSN: 0887-2333

hal-01141725
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725/file/Polarized%20location%20of%20SLC.pdf
DOI : 10.1016/j.tiv.2015.03.019

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
Les risques hydrométéorologiques sont au coeur du programme de recherche HyMeX sur le cycle de l'eau en Méditerranée. HyMeX a pour ambition d'améliorer leur prévision, de mieux connaître leur variabilité à l'échelle pluriannuelle et de renseigner sur leur évolution, dans le contexte du changement climatique. À cette fin, deux campagnes de mesures dédiées à ces événements extrêmes (SOP1 : pluies intenses et crues rapides ; SOP2 : vents forts et formation d'eaux denses) sont organisées en Méditerranée nord-occidentale, entre septembre 2012 et mars 2013.
ISSN: 0026-1181

hal-00829760
https://hal.archives-ouvertes.fr/hal-00829760
DOI : 10.4267/2042/48793

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
D'initiative française, le projet international HyMeX a pour objectif d'améliorer la compréhension du cycle de l'eau en Méditerranée, de sa variabilité, de l'échelle de l'événement météorologique aux échelles saisonnières et interannuelles, et de ses caractéristiques sur une décennie, dans un contexte de changement global. Le projet est motivé par le rôle déterminant des processus de mésoéchelle, couplés entre l'atmosphère, la mer et la terre, sur la variabilité du système climatique et sur le déclenchement d'événements hydrométéorologiques extrêmes (précipitations et inondations, vents forts et convection océanique, canicules et sécheresses). Le projet vise enfin à évaluer les conséquences de ces événements extrêmes sur la vulnérabilité sociale et économique de cette région et sa capacité d'adaptation.
ISSN: 0026-1181

hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758/document
https://hal.archives-ouvertes.fr/hal-00828758/file/meteo_2013_80_23%20%281%29.pdf
DOI : 10.4267/2042/48792

Éditeur(s) : HAL CCSD
Résumé : International audience
AGU Fall Meeting 2010
San Francisco, United States

insu-01133022
https://hal-insu.archives-ouvertes.fr/insu-01133022

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
Interleukin (IL)-33 is crucially involved in liver pathology and drives hepatoprotective functions. However, the regulation of IL-33 by cytokines of the IL-6 family, including oncostatin M (OSM) and IL-6, is not well studied. The aim of the present study was to determine whether OSM mediates regulation of IL-33 expression in liver cells. Intramuscular administration in mice of an adenovirus encoding OSM (AdOSM) leads to increase in expression of OSM in muscles, liver, and serum of AdOSM-infected mice compared with control mice. The increase of circulating OSM markedly regulated mRNA of genes associated with blood vessel biology, chemotaxis, cellular death, induction of cell adhesion molecules, and the alarmin cytokine IL-33 in liver. Steady-state IL-33 mRNA was upregulated by OSM at an early phase (8 h) following AdOSM infection. At the protein level, the expression of IL-33 was significantly induced in liver endothelial cells [liver sinusoidal endothelial cells (LSEC) and vascular endothelial cells] with a peak at 8 days post-AdOSM infection in mice. In addition, we found OSM-stimulated human microvascular endothelial HMEC-1 cells and human LSEC/TRP3 cells showed a significant increase in expression of IL-33 mRNA in a dose-dependent manner in cell culture. The OSM-mediated overexpression of IL-33 was associated with the activation/enrichment of CD4(+)ST2(+) cells in liver of AdOSM-infected mice compared with adenovirus encoding green fluorescent protein-treated control mice. In summary, these data suggest that the cytokine OSM regulates the IL-33 expression in liver endothelial cells in vivo and in HMEC-1/TRP3 cells in vitro and may specifically expand the target CD4(+)ST2(+) cells in liver
ISSN: 0193-1857

hal-01220632
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01220632
DOI : 10.1152/ajpgi.00398.2014
PUBMED : 26251474

Éditeur(s) : HAL CCSD
Résumé : International audience
Geophysical Research Abstracts
Vienne, Austria

insu-00609966
https://hal-insu.archives-ouvertes.fr/insu-00609966

Éditeur(s) : HAL CCSD
Résumé : National audience
Journées IODP France
Paris, France

insu-01133126
https://hal-insu.archives-ouvertes.fr/insu-01133126