Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia Auteur(s) : Castex, Mathieu Chim, Liet Pham, Dominique Lemaire, Pierrette Wabete, Nelly Nicolas, Jean-louis Schmidely, P Mariojouls, C Éditeur(s) : Elsevier Résumé : We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases in the survival rate (7% and 15% respectively) and final biomass (8% and 12% respectively). No differences were recorded among treatments on growth performances, but significant lower food conversion ratios were obtained with probiotic treatment. After 5 weeks of rearing, the Hepatosomatic Index and the adjusted dry weight of the digestive gland were significantly increased by 10% and by 9% respectively in shrimps treated with probiotic. In the meantime, the specific activities of alpha amylase and trypsin in the digestive gland showed a significant effect of the probiotic treatment with respective increases by 35% and 55%. The rise in total trypsin activity following morning feeding was also enhanced by the probiotic treatment (P < 0.001). The highest concentration of probiotic (between 10(4)-10(5) CFU g(-1) of fresh gut) in the shrimp gut was obtained 2 h after feeding. The concentration remained high for 4 h after feeding before decreasing until the next meal. Total bacterial counts on Marine agar and TCBS in the gut were significantly lowered by the probiotic treatment over the 10 weeks of the experiment. Additionally, during the first 2 weeks, prevalence and load of V nigripulchritudo strains in haemolymph was lower in animals fed with the probiotic diet. This study demonstrated, under pond conditions, that feeding shrimp with live terrestrial lactic acid bacteria can be an effective treatment for improving shrimp culture affected by vibriosis. (c) 2008 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2008-03 , Vol. 275 , N. 1-4 , P. 182-193 Droits : 2008 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/2008/publication-4079.pdf DOI:10.1016/j.aquaculture.2008.01.011 http://archimer.ifremer.fr/doc/00000/4079/ | Partager |
Remplacement total des artémia par des microparticules inertes dans l'élevage larvaire de l'ombrine Sciaenops ocellatus en Martinique Auteur(s) : Petton, Bruno Falguiere, Jean-claude Éditeur(s) : Proceedings of the 59th Annual Gulf and Caribbean Fisheries Institute Résumé : In most of marine fishes cultured, larvae in their early development are fed with live prey (rotifer and/or artemia). In hatcheries, culture of these preys is labour consuming, needs specific facilities and represent an important part of production cast. On the other hand, world artemia production is subjected to fluctuation of harvest in the wild which could Iimit fish farming industry in the near future. A dried formula adapted to marine fish larvae has been perfected by Ifremer's center of Brest (France) and is distributed by a feeding company since 1999. This artificial microbound diet has been successfully tested on several temperate fishes such as European seabass (Dicentrarchus labrax), Atlantic cod (Gadus morhua) or European seabream (Sparus aurata) in a co-feeding (i.e. artemia and dried diet) strategy. Experiments described here were carried out ta evaluate the performances obtained during total substitution of artemia by this diet in Red drum (Sciaenops ocellatus) larval culture. ln 2004 and 2005, four experiments were conducted on feed technology aspects (experimental or industrial manufacturing), weaning strategy (direct or co-feeding) and feed distribution method (prototypes or classical self feeder) in comparison to the standardized method using live preys. At the end of larval phase (day 15) and at day 30, end of nursery phase (0.6 g), survival relative to control (RTC) was respectively 96.9% and 111.0% in the best treatment. Concerning growth, the weight RTC reached at day 15 and day 30 was respectively 82.8% and 72.4%. However, it is important to note that rearing protocols have to be adapted ta the use of these micro pellets in order toprevent degradation of culture quality. These results show that this new product can be applied on industrial scale in private hatcheries. But further research is needed ta evaluate replacement of rotifers by this dried formula in order to completely eliminate live prey in larval culture of Red drum. Dans la plupart des poisons marins élevés, les larves sont nourries avec des proies vivantes (rotifères et/ou artémia) au début de leur développement. Dans les écloseries, la culture de ces proies est consommatrice de main d'oeuvre, nécessite des installations spécifiques et entre pour une part importante dans le coût de production. Par ailleurs, la production mondiale d'artémia est dépendante de la fluctuation des récoltes dans le milieu naturel, ce qui pourrait gêner la filière piscicole marine dans un futur proche. Une formulation alimentaire inerte adaptée aux larves de poisson marin a été mise au point par le centre Ifremer de Brest (France) et est commercialisée par un provendier depuis 1999. Ces microparticules artificielles ont été testée avec succès sur plusieurs poissons tempérés comme le bar européen (Dicentrarchus labrax), la morue atlantique (Gadus morha) ou la daurade européenne (Sparus aurata) dans un contexte de co-feeding (i.e. artémia et aliment inerte). Les expériences décrites ici ont été menées afin d'évaluer les performances obtenues lors de la substitution totale des artémia par cet aliment dans l'élevage larvaire de l'ombrine (Sciaenops ocellatus). En 2004 et 2005, quatre expériences ont été menées sur l'aspect technologie alimentaire (fabrication expérimentale ou industrielle), la stratégie de sevrage (direct ou co-feeding) et le mode de distribution de l'aliment (distributeur classique ou prototypes) en comparaison au standard sur proies vivantes. En fin de phase larvaire (jour 15) et au jour 30 après une phase de prégrossissement (0.6g), la survie par rapport au témoin (RTC) était respectivement de 96.9% et 111 .1% dans le meilleur traitement. Concernant la croissance, les poids obtenus aux jours 15 et 30 atteignaient respectivement 82.8% et 72.4%. Cependant il est important de relever que les protocoles d'élevage doivent être adaptés à l'utilisation de ces microparticules afin de prévenir une dégradation de la qualité d'élevage. Ces résultats montrent que ce nouveau produit peut être appliqué à l'échelle industrielle dans les écloseries privées. Cependant, les recherches doivent être poursuivies pour évaluer les possibilités de remplacement des rotifères par ces aliments inertes afin de s'affranchir complètement de l'utilisation de proies vivantes dans l'élevage larvaire de l'ombrine. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/2006/acte-6597.pdf http://archimer.ifremer.fr/doc/00000/6597/ | Partager Voir aussi micro diets Sciaenops ocellatus larval culture aquaculture microparticules Sciaenops ocellatus élevage larvaire aquaculture Télécharger |
Selection of finfish species (seabass) as candidates for aquaculture in French Polynesia Auteur(s) : Fuchs, Jacques Gasset, Eric Nedelec, Georges Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 43, pp.461-484 Résumé : Species with a very high potential for aquaculture, allowing development projects on a short terms basis: the seabass originating from S.E. Asia. Species, potentially interesting but presenting some drawbacks, as difficulties to perform larval rearing or presenting pathological problem. Species considered as difficult to rear and not retained like Siganus argenteus , Siganidae or Caranx ignobilis , Carangidae. The future development of finfish in Polynesia is emphasized and a project of sea bass netcage culture in Polynesian lagoon is presented. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1989/acte-1474.pdf http://archimer.ifremer.fr/doc/00000/1474/ | Partager Voir aussi Siganus argenteus Oreochromis Coryphaenidae Epinephelus microdon Serranidae Lates calcarifer Centropomidae Pisces Fry Fish larvae Télécharger |
Premiers essais d'élevage larvaire de l'ombrine subtropicale (red fish) Sciaenops ocellatus dans les conditions intensives en Martinique Auteur(s) : Soletchnik, Patrick Thouard, Emmanuel Goyard, Emmanuel Baisnee, D Baisnee, D Baisnee, D Yvon, C Baker, P Résumé : Two batches of red drum larvae were air shipped from hatcheries in the U.S.A. and stocked in two intensive tank experiments at densities oc 7 :.nd 2-3 larvae per liter. Food items include live rotifers, copepods, brine shrimps. squid and commercial fish pellets. Growth rates were similar in both experiments up to day 20 (12-1" mm) but differences in feeding schedules and an infestation by the dinoflagelate Amyloodinium sp. affected the growth from day 20 to 43 in the second trial. Significant mortalities occurred in the tanks during the first feeding period (days 2 to 7) and again from days 17 to 20 as the result of cannibalism. Survival between day 2 and day 44 was 44% in the first trial. Abnormal inflation of the swim bladder and infection with Amyloodinium sp. reduced the survival to 6-19% in the second. Deux lots de larves du tambour rouge ont été transportés par avion depuis des écloseries aux USA et stockés dans deux essais en bac intensif aux densités oc 7 : nd 2-3 larves le litre. L'alimentation comprend les rotifères vivants, les copépodes, les artémias, les encornets et les boulettes de poisson commerciales. Les taux de croissance étaient pareils dans les deux expériences jusqu'au jour 20 (12-1''mm) mais des différences dans les programmes d'alimentation et une infestation par le dinoflagellé Amyloodinium sp. ont modifié la croissance à partir du jour 20 jusqu'au jour 43 dans le deuxième essai. Des mortalités importantes se sont produites dans les bacs pendant la première période d'alimentation (jours 2 et 7) et encore du jour 17 jusqu'au 20 à la suite d'un cannibalisme. La survie entre le jour 2 et le jour 44 était de 44 % dans le premier essai. Un gonflement anormal de la vessie natatoire et une infection de l'Amyloodinium sp. ont réduit la survie de 6 ¿ 19 % dans le deuxième. Droits : 1988 Ifremer, UAG, Orstom http://archimer.ifremer.fr/doc/1998/rapport-1914.pdf http://archimer.ifremer.fr/doc/00000/1914/ | Partager |
Shrimp aquaculture in New Caledonia Auteur(s) : Galinie, C Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 5, pp.45-50 Résumé : In New Caledonia, there is no indigenous shrimp species for commercial aquaculture and it is necessary to control the complete cycle in captivity. Since 1973 and the creation of the Station d'Aquaculture de Saint-Vincent (S.A.S.V.), a joint project between IFREMER, France-Aquaculture and the territory of New Caledonia, nine species have been tested and for one of these, P. stylirostris , (introduced in 1980), we are now in a commercial scale production with the tenth generation obtained in captivity. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1989/acte-1496.pdf http://archimer.ifremer.fr/doc/00000/1496/ | Partager Voir aussi New Caledonia Penaeus stylirostris Rearing Stocking density Larvae Growth Pellet feeds Intensive culture Télécharger |
Could a diet enriched with n-3 highly unsaturated fatty acids be considered a promising way to enhance the immune defences and the resistance of Penaeid prawns to environmental stress? Auteur(s) : Chim, Liet Lemaire, Pierrette Delaporte, Maryse Le Moullac, Gilles Galois, Robert Martin, Jean-louis Éditeur(s) : Blackwell science Résumé : The prawn Penaeus stylirostris (Stimpson), when fed for 28 days with n-3 highly unsaturated fatty acid (HUFA)-enriched feed pellets, demonstrated an enhanced resistance to variations in environmental parameters (a decrease in temperature and salinity over a 4-day period from 28 degreesC to 17 degreesC and from 35 parts per thousand to 10 parts per thousand respectively) and an improvement in their immune defence potential, i.e. increased agglutination titre of plasma and increased respiratory burst of haemocytes. Aquaculture Research (1355-557X) (Blackwell science), 2001-02 , Vol. 32 , N. 2 , P. 91-94 Droits : 2001 Blackwell http://archimer.ifremer.fr/doc/2001/publication-606.pdf DOI:10.1046/j.1365-2109.2001.00534.x http://archimer.ifremer.fr/doc/00000/606/ | Partager |