Observation sur la maturation et la reproduction en captivité des crevettes peneides en milieu tropical Auteur(s) : Michel, A Éditeur(s) : Publ. CNEXO (France) (Actes Colloq.)(no. 7) Résumé : The present development of Peneid shrimp culture is limited by the problem set by the routine obtainment of reproduction in captivity. In Polynesia where no species of commercial interest is found naturally, this obtainment is a necessary prerequisite. The observations at the Centre Océanologique du Pacifique of CNEXO were made on 6 species of the genus Penaeus (P. merguiensis, P. aztecus, P. japonicus, P. monodon, P. vannamei and P. stylirostris) raised and maintained in 12 m² outdoortanks. At temperatures, between 25 and 32°, a salinity of 35 and a pH of 8,2, maturations and spawnings are seen throughout the year and several generations were obtained : P. merguiensis, F7 ; P. aztecus et P. japonicus, F3 ;P. monodon, F2 ; P. stylirostris and P. vannamei F1. For P. aztecus et P. mondon maturation is induced by unilateral eyestalk ablation. The animals behavior copulation and external signs of ovarian, development are described. The factors which seem essential to the proper progress of maturation are the temperature, the light intensity, the food and the state of health of the animals. Le développement des élevages de crevettes Pénéidés est actuellement limité par les problèmes que pose l'obtention en routine de la reproduction en captivité. En Polynésie où aucune espèce d'intérêt commercial ne se trouve à l'état naturel, cette obtention était un préalable nécessaire. Les observations effectuées au Centre Océanologique du Pacifique du CNEXO ont porté sUr 6 espèces du genre Penaeus (P. merguiensis, p. aztecus, P. japonicus, P. monodon, P. vannamei et P. stylirostris) élevées et maintenues dans des bacs extérieurs de 12 m² en circulation ouverte. Avec des températures variant de 25 à 32°, une salinité de 35 et un pH voisin de 8,2, maturations et pontes s'obserVent toute l'année et plusieurs générations ont été obtenues : P. merguiensis, F7 ; P. aztecus et P. japonicus, F3 ;P. monodon, F2 ; P. stylirostris et P. vannamei, F1. Pour P. aztecus et P. mondon, la maturation est induite par épédonculation unilatérale. Le comportement des animaux, la copulation et les signes extérieurs du développement des ovaires sont décrits. Les facteurs qui paraissent essentiels au bon déroulement du processus de maturation sont la température l'éclairement, l'alimentation et l'état de santé des animaux Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1977/publication-6073.pdf http://archimer.ifremer.fr/doc/00000/6073/ | Partager |
Combined effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris Auteur(s) : Lemaire, Pierrette Bernard, E Martinez-paz, J Chim, Liet Éditeur(s) : Elsevier Résumé : In New Caledonia, the "syndrome 93," which results in mass mortalities of farmed shrimp Penaeus stylirostris, occurs during the transitional and the cold seasons. The transitional season is characterized by an important fall in the average water temperature from 28 to 19 degreesC in 2 weeks. Besides this marked temperature fall, there are also nyctemeral temperature changes of up to 7 degreesC in 12 h. The cold season is characterized by an average water temperature of 19 degreesC with extreme lows of 14 degreesC. The objective of this study was to determine the effect of temperature and its variations on the osmoregulatory capacity of juvenile (7-10 g average weight) and subadult (25 g average weight) shrimps subjected to conditions of different salinity. A reduction in temperature level resulted in a decreased osmoregulatory capacity (OC) (defined as the difference between the osmolality of the haemolymph and that of sea water) at low salinity (hyper-CO) and at high salinity (hypo-CO), respectively, below and above the isoosmotic point (26.2 ppt). In subadult shrimps, the hyper-CO was affected when temperature dropped from 26 to 22 degreesC. The hypo-CO was modified only when temperature decreased down to 15 degreesC. Furthermore, the sensitiveness of osmoregulation to temperature changes was dependent on the developmental stage of the shrimp. Subadults were more sensitive than juvenile animals. The value of the isoosmotic point, which did not depend on the shrimp developmental stage, increased when temperature decreased to 17 or 15 degreesC. Our results provide an additional explanation of the "syndrome 93" and display the unfavourable influence of marked temperature drop on shrimp health. Our research could provide a possibility of seeking a nutritional and/or genetic solution to improve the capacity of the shrimps to maintain their homeostasis within a rearing medium with fluctuating temperatures. Aquaculture (0044-8486) (Elsevier), 2002-06 , Vol. 209 , N. 1-4 , P. 307-317 Droits : 2002 Elsevier Science B.V. All rights reserved http://archimer.ifremer.fr/doc/2002/publication-395.pdf DOI:10.1016/S0044-8486(01)00756-6 http://archimer.ifremer.fr/doc/00000/395/ | Partager |
Etude des tris précoces en élevage de crevettes P. stylirostris dans un objectif de sélection génétique. Février à Juillet 2000. Auteur(s) : De Decker, Sophie Résumé : Schemes optimisation for genetic improvement for growth of Penaeus stylirostris requires the development of technologies for grading shrimps easily. This work shows that bar and mesh graders are very suitable for early selection at post-larval stages, as long as early growth is heritable. Experimental data sets lead to the equations which give the grading weight as a function of the dimensions of the graders. Mesh graders which allow an earlier selection than bar graders (PL19 instead of PL25) open a new way to implement experimental selection into production hatcheries. L’optimisation des schémas d’amélioration génétique de la croissance de Penaeus stylirostris, passe nécessairement par la mise au point des techniques de tri rapide et facile à mettre en oeuvre par les acteurs de la filière. Ce travail montre que les trieurs à barreaux ou à maille sont des outils adaptés à une sélection précoce en sortie d’écloserie, dans la mesure où la croissance précoce s’avèrerait héritable. Les tris expérimentaux ont permis d’établir les formules permettant de calculer les poids de tris en fonction des dimensions des trieurs. Les trieurs à maille qui permettent de trier les crevettes dès PL19 ouvrent des perspectives intéressantes pour intégrer une démarche de sélection expérimentale au sein des écloseries de production. Droits : 2000 Ifremer http://archimer.ifremer.fr/doc/00203/31439/29836.pdf http://archimer.ifremer.fr/doc/00203/31439/ | Partager Voir aussi tri sélection génétique crevettes Penaeus stylirostris grading sorter genetic selection shrimps Penaeus stylirostris Télécharger |
Aquaculture activities in Fiji Auteur(s) : Vereivalu, T Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 3, pp.31-36 Résumé : Fiji has no aquaculture tradition, but the Government is engaged in aquaculture production since 1969, with the help of other countries and international organization like F.A.O. Some species have been tested: Fishes: Grass carp (Ctenopharyngodon idellus ) and Tilapia ; Molluscs: Oyster (Crassostrea echinata ) and Mussel (Mytilus viridis ); Crustacean: freshwater prawn (Macrobrachium rosenbergii ) and shrimps (Penaeus monodon, P. indicus, P. stylirostris ). Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1989/acte-1498.pdf http://archimer.ifremer.fr/doc/00000/1498/ | Partager |
Cross breeding of different domesticated lines as a simple way for genetic improvement in small aquaculture industries: Heterosis and inbreeding effects on growth and survival rates of the Pacific blue shrimp Penaeus (Litopenaeus) stylirostris Auteur(s) : Goyard, Emmanuel Goarant, Cyrille Ansquer, Dominique Brun, Pierre De Decker, Sophie Dufour, Robert Galinie, C Peignon, Jean-marie Éditeur(s) : Elsevier Résumé : Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two successive generations. Results were very consistent: F-1-hybrids growth rates in earthen ponds were 37% (+/-7% SD) higher than for pure populations. Cage experiments demonstrated no competition between the different populations when reared together or separately in a common environment. The F-1-hybrids also showed better survival rates in all experiments. Combining the results on growth and survival rates leads to the conclusion that biomass production is much higher with F-1-hybrid populations than with pure populations using the same quantity of juveniles stocked: biomass production in ponds was increased 1.4 and 2.3 times on year I and year 2 respectively, and 1.9 times in floating cages. The advantage of growing F-1-hybrids appeared proportionally higher when environmental and sanitary conditions led to poorer survival (34% in year 2 vs. 56% in year 1). These results are a good example of performance improvement by heterosis effect and/or of performance loss due to inbreeding in the pure populations. This study demonstrates that aquaculture industries which cannot afford large selection programs may benefit from using two different inbred parental stocks to produce F-1-hybrids for each commercial growout. This is notably true when only inbred populations are available, or when introduction of genetic variability from the wild or from other genetic resources represents a zoo-sanitary risk. In our case, the expected increase in L stylirostris production could be around 85% (according to our average results) if producers keep stocking their ponds at their current densities using F-1-hybrids. However, for sustainability reasons, it is advisable to stock F-1-hybrid animals at lower densities, the gain in performance allowing producing the same amount of biomass with less input. (C) 2008 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2008-06 , Vol. 278 , N. 1-4 , P. 43-50 Droits : 2008 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/2008/publication-4316.pdf DOI:10.1016/j.aquaculture.2008.03.018 http://archimer.ifremer.fr/doc/00000/4316/ | Partager Voir aussi Vibriosis Survival Growth Penaeus Litopenaeus stylirostris Shrimp Cross breeding Genetic improvement Télécharger |
Toxic factors of Vibrio strains pathogenic to shrimp Auteur(s) : Goarant, Cyrille Herlin, Jose Brizard, Raphael Marteau, Anne-laure Martin, C Martin, B Éditeur(s) : Inter-research Résumé : Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20 degrees C) and not at higher temperature (30 degrees C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria. Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2000-03 , Vol. 40 , N. 2 , P. 101-107 Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/00000/10543/7600.pdf DOI:10.3354/dao040101 http://archimer.ifremer.fr/doc/00000/10543/ | Partager |
Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia Auteur(s) : Saulnier, Denis Avarre, Jean-christophe Le Moullac, Gilles Ansquer, Dominique Levy, Peva Vonau, Vincent Éditeur(s) : Inter-Research Résumé : Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V. penaeicida was developed. It gave an expected amplicon of approximately 310 bp in ethidium bromide-stained agarose gels. The specificity of these primers was assessed with different Vibrio species. Furthermore, DNA extracted by the ChelexTM method could be used to detect fewer than 20 cultured Vibrio cells in seawater or shrimp hemolymph by this assay. It appears to be a reliable screening method for detecting V. penaeicida in shrimp and from the aquatic environment. Diseases of aquatic organisms (0177-5103) (Inter-Research), 2000-03 , Vol. 49 , N. 2 , P. 109-115 Droits : Inter-Research 2000 http://archimer.ifremer.fr/doc/2000/publication-4436.pdf DOI:10.3354/dao040109 http://archimer.ifremer.fr/doc/00000/4436/ | Partager |
Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris Auteur(s) : Bierne, Nicolas Bezuart, Ivan Vonau, Vincent Bonhomme, François Bedier, Edouard Éditeur(s) : Elsevier Résumé : Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the positive correlation was not significant, but the combined test of single locus heterozygote advantage over the three loci was significant (P = 0.005).Use of DNA microsatellite markers, whose selectively neutral status is generally accepted, allowed us to reject the hypothesis of direct overdominance at marker loci as an explanation for an association of growth rate with heterozygosity. The average depression associated with one locus in our case is above or equivalent to the amount observed for a 10% increase of inbreeding in other species. These results suggest that heterozygosity at neutral marker loci is sufficiently well correlated with individual inbreeding coefficients to reveal a significant residual inbreeding load for growth rate in Tahitian P. stylirostris stocks. Inbreeding during hatchery propagation was, thus, insufficient to purge the ancestral load. Aquaculture (0044-8486) (Elsevier), 2000-04 , Vol. 184 , N. 3-4 , P. 203-219 Droits : 2000 Elsevier Science B.V. All rights reserved http://archimer.ifremer.fr/doc/2000/publication-753.pdf DOI:10.1016/S0044-8486(99)00331-2 http://archimer.ifremer.fr/doc/00000/753/ | Partager Voir aussi Penaeus stylirostris Heterozygosity Marker associated heterosis Inbreeding depression Microsatellites Télécharger |
Traitement des crevettes contre le noircissement .Mise au point d'une ligne de traitement Auteur(s) : Chantreau, Patrick Résumé : Once fished, the shrimps must be cooled quickly in water cooled in order to slow down all chemical and bacteriological activities.
In addition, in order to avoid blackening (mélanose) shrimps (céphalothorax and legs) it is necessary to treat those with an antioxydant product.
The product currently used to inhibit this enzymatic change is the soda niétabisuifite (Na-> S7 O5), until now, after being fished. the shrimps were directly soaked in a cooled water tank, containing the pesticide.
The solution of treatment was progressively prepared needs. The concentration of this one was approximately 2 to 3% of métabisulfite, that is to say 6 to 9 G of SO2 per liter for a starting product containing 320 G of SO2 approximately.
In order to preserve a good quality of the liquid of treatment it was essential to often renew it (every 400 to 500 kg) what was not always the case, the métabisulfite being often added progressively with the liking of the appointed person with the treatment of shrimps.
In order to cure this delicate problem of renewal of vat and uniformity of the treatment, the SODACAL obtained the system of treatment by sprinkling. Une fois pêchées, les crevettes doivent être refroidies rapidement dans de l'eau réfrigérée afin de ralentir toutes activités chimiques et bactériologiques. D'autre part, afin d'éviter le noircissement (mélanose) des crevettes (céphalothorax et pattes) il est nécessaire de traiter celles-ci avec un produit anti-oxydant. Le produit actuellement utilisé pour inhiber cette altération enzymatique est le niétabisuifite de soude (Na-> S7 O5), jusqu'à maintenant, après avoir été pêchées. les crevettes étaient directement trempées dans une cuve d'eau réfrigérée, contenant le produit de traitement. La solution de traitement était préparée au fur et à mesure des besoins. La concentration de celle-ci était d'environ 2 à 3 % de métabisulfite, soit 6 à 9 g de SO2 par litre pour un produit de départ contenant 320 g de SO9 environ. Afin de conserver une bonne qualité du liquide de traitement il était indispensable de le renouveler souvent (tous les 400 à 500 kg) ce qui n'était pas toujours le cas, le métabisulfite étant souvent rajouté au fur et à mesure au gré de la personne préposée au traitement des crevettes. Afin de remédier à ce problème délicat de renouvellement de bac et d'uniformité du traitement, la SODACAL s'est dotée du système de traitement par aspersion. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1991/rapport-1648.pdf http://archimer.ifremer.fr/doc/00000/1648/ | Partager Voir aussi New Caledonia Blackening Treatment Penaeus stylirostris Shrimp Nouvelle Calédonie Noircissement Traitement Penaeus stylirostris Crevette Télécharger |
Penaeid reared brood stock : closing the cycle Auteur(s) : AQUACOP Éditeur(s) : 10 th Meeting of the World Mariculture Society. Honolulu, January 1979 Résumé : Three shrimp species, Penaeus monodon, P. stylirostris (Panama and Mexican strains) and P. vannamei have matured and spawned in captivity at the Centre Océanologique du Pacifique (COP) laboratory. The shrimp, originally stocked as juveniles or postlarvae, were reared to adult size in tanks and ponds and have completed the cycle: F3 generation for P. monodon, F2 for P. stylirostris (Panama strain) and P. vannamei and F1 for P. stylirostris (Mexican strain). Maturation occurs naturally under our rearing conditions or is induced by unilateral eyestalk ablation. Details are given on mating behavior, ovarian development, number of spawnings, egg viability and maintenance of captive brood stock. Partial results so far obtained for P. monodon and P. stylirostris clearly show a reared brood stock could sustain a commercial hatchery. [NOT CONTROLLED OCR] Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1979/acte-4423.pdf http://archimer.ifremer.fr/doc/00000/4423/ | Partager |
Acquisition of susceptibility to Vibrio penaeicida in Penaeus stylirostris postlarvae and juveniles Auteur(s) : Goarant, Cyrille Regnier, France Brizard, Raphael Marteau, Anne-laure Éditeur(s) : Elsevier Résumé : Vibriosis is a major disease problem in shrimp aquaculture, affecting all developmental stages, from larvae in hatchery tanks to juveniles and broodstock in growout ponds. However, bacterial strains responsible for vibriosis in the successive stages are usually considered to be different, and virulence specificity has been reported both at the species and at the stage levels. The so-called « Syndrome 93 » is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Penaeus stylirostris in growout ponds and broodstock tanks in New Caledonia. This pathology does not cause any mortality in hatchery or nursery phases. An experimental infection design using balneation of postlarvae and early juveniles in V. penaeicida suspensions was used to evaluate the developmental stage at which shrimp become sensitive to this vibriosis. We demonstrated that the acquisition of susceptibility to this pathogen is very sudden and correlated with the acquisition of the definitive rostral formula, and from this draw conclusions regarding virulence mechanisms of V. penaeicida in P. stylirostris. Aquaculture (Elsevier), 1998-12 , Vol. 169 , N. 3-4 , P. 291-296 Droits : 1998 Elsevier Science B.V. All rights reserved http://archimer.ifremer.fr/doc/1998/publication-383.pdf DOI:10.1016/S0044-8486(98)00380-9 http://archimer.ifremer.fr/doc/00000/383/ | Partager |
Ten years of experimental rearing of Penaeid shrimp in Tahiti and New Caledonia (South Pacific) Éditeur(s) : Proceedings of the 15 th Meeting World Mariculture Society, Vancouver Résumé : 5ince 1973, the "Centre Océanologique du Pacifique" has been conducting in its Tahitian and New-Caledonian facilities, tropical penaeid shrimp rearings. Up to now, among the different species tested, Penaeus monodon, P. stylirostris, P. indicus and P. vannamei have been selected and currently cultured for several generations. [NOT CONTROLLED OCR] Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1984/acte-4444.pdf http://archimer.ifremer.fr/doc/00000/4444/ | Partager |
La biosécurité à l'Ifremer LEAD,station de Saint-Vincent Auteur(s) : Herlin, Jose Résumé : Penaeus stylirostris shrimp imported from Hawaii for a genetic program and kept at the Saint-Vincent research facility had progressively developed a high sensitivity to IHHNV, the etiological agent of the Infectious, Hypodermic, and Hematopoietic Necrosis. Fears were that the virus could not only be transferred to their offspring, but also that the New Caledonian strain of Penaeus stylirostris, so far considered as resistant, could become sensitive by an off balance of its hosts/pathogen equilibrium. In October 2008, a joint decision between Uprac (shimp owner), Davar (New Caledonian Animal Health Authority) and Ifremer (scientific partner) was taken to terminate all pure breed and hybrid Hawaiian populations remaining in the ponds, and to implement an intensive fallowing protocol, and controlled restocking. IHHNV was also suspected to trouble experiments carried out in the research facility (mortalities, deformities, slow growth), thus affecting research programs. Difficulties pointed out by IHHNV have triggered since 2008 some awareness on biosecurity issues at some level of the New Caledonian shrimp industry. At the St-Vincent research station, it led to a risk assessment and implementation of biosecurity procedures (fallowing, propagation, grow out and use of screened animals) which minimizes the risk of expression of IHHNV.
The use of a brand new hatchery building on site also helped in resetting routine hygiene procedures during production and in controlling incoming and outgoing items (shrimp, water, feed, personnel, equipment, pests and wastes).
IHHNV has not been detected on analysed samples since then nor have any symptoms been found during experimental or broodstock growout. Keeping this control alive is considered as a useful tool to lower the risk of potential health threats, to get a better understanding in case of an outbreak, and to increase the reliability of experimental results. Les crevettes originaires d’Hawaii et conservées au LEAD St-Vincent, dans le cadre du programme d’introduction de variabilité génétique dans le cheptel Calédonien, ont progressivement montré une sensibilité importante au virus IHHNV, agent de la Nécrose Hypodermale et Hématopoïétique Infectieuse (mortalité, croissance ralentie, déformations). Les risques d’une transmission génétique de cette sensibilité à leur descendance à moyen terme, et de son développement par rupture de l’équilibre hôte/pathogène sur la souche dite « Calédonienne » jusqu’alors considérée comme résistante à l’IHHN, ont conduit à partir d’octobre 2008, avec l’accord et l’appui de l’UPRAC et de la DAVAR, à la suppression des animaux hawaiiens ou hybrides encore présents dans les bassins de terre du LEAD St-Vincent, au vide sanitaire de ces derniers, et au repeuplement contrôlé du site. D’autre part, le virus IHHN est soupçonné d’avoir pu affecter les animaux utilisés dans les expérimentations menées au LEAD-St-Vincent (mortalités, déformations, croissance réduite), influençant donc potentiellement leur bon déroulement et celui des programmes de recherches. Les obstacles soulevés par l’expression du virus IHHN ont donc initié depuis 2008 une prise de conscience et la mise en place de démarches de biosécurité dans certains points de la filière. A la station de St-Vincent, cette démarche s’est traduite par une identification/évaluation des risques et l’application de mesures (vide sanitaire, reproduction, élevage et utilisation des animaux contrôlés) chacune dotée de procédures qui regroupées, minimisent le risque d’introduction et de dissémination de l’IHHNV. La prise en main de la nouvelle structure d’écloserie sur le site de la SASV a également été mise à profit pour revoir les procédures d’hygiène de routine dans la conduite des productions et dans le contrôle des flux intrants et sortants (animaux, eau, aliments, personnel, équipement, nuisibles, déchets). Aucune détection d’IHHNV sur les échantillons prélevés n’a depuis été observée, de même qu’aucun symptôme de l’expression de cette maladie sur les animaux en élevage sur le site de Saint-Vincent. La poursuite de ces mesures est envisagée comme un outil d’aide à la minimisation des problèmes potentiels, à leur compréhension en cas d’apparition, et à la fiabilisation des résultats. Droits : 2010 Ifremer http://archimer.ifremer.fr/doc/00118/22949/20776.pdf http://archimer.ifremer.fr/doc/00118/22949/ | Partager |
Expériences 97 sur l'influence des sédiments du bassin d'élevage sur la physiologie de Penaeus stylirostris Auteur(s) : Cochard, Jean-claude Résumé : Les crevettes pénéides maintenues captives dans des cages posées sur le fond des bassins d'élevage subissent un stress dû au sédiment qui peut être évalué par la comparaison entre la valeur de la pression osmotique de leur hémolymphe et celle des crevettes libres du même bassin. Les objectifs des travaux réalisés à la station de Saint-Vincent (SASV, Nouvelle Calédonie) étaient de confirmer la variabilité spatiale de l'intensité du stress et de rechercher dans les sédiments un indicateur physique ou chimique corrélé à ce stress. Des lots de 25 à 30 crevettes de 13,2g, pêchées à l'épervier, ont été placés dans des cages de grillage métallique déposées en 12 points du bassin d'élevage. Les cages ont été relevées au bout de 24 heures et la pression osmotique des crevettes au stade d'inter-mue C comparée à celles d'individus libres pêchés le même jour. L'expérience ayant été renouvelée 4 fois, le plan expérimental était constitué de 4 blocs de 12 cages. Les caractéristiques du sédiment ont été étudiées sur deux séries de mesures in situ (pH de l'eau interstitielle, potentiel d'oxydoréduction) ou de prélèvements (matière sèche, taux de matière organique, teneur en azote ammoniacal) réalisés à proximité immédiate du point de dépôt des cages. L'étude révèle l'existence d'un gradient significatif d'intensité du stress chez les crevettes captives, elle a permis de détecter une corrélation linéaire très significative entre le pH de surface des sédiments et la capacité osmorégulatrice des crevettes. Ces observations ont été confirmées par une étude expérimentale réalisée à Tahiti au Centre Océanologique du Pacifique. Droits : 1997 Ifremer http://archimer.ifremer.fr/doc/00131/24265/22263.pdf http://archimer.ifremer.fr/doc/00131/24265/ | Partager Voir aussi Penaeus stylirostris capacité osmorégulatrice stress sédiment aquaculture Crustacés. crevettes pénéides pH Télécharger |
Les échanges internationaux de crevettes pénéidés vivantes entre les pays du Pacifique, de l'Atlantique et de l'Europe Auteur(s) : Michel, Alain Éditeur(s) : Office Int Epizooties Résumé : The tremendous development of penaeid shrimp culture across the world over the past twenty years has led to international trade in eggs, larvae and spawners of the best shrimp species for aquaculture. Trade has involved, in particular, the following species: - Penaeus japonicus from Japan to Europe, the Pacific Islands and South America - P. monodon from South-East Asia to almost all tropical countries - P. vannamei and P. stylirostris from countries along the Pacific coast of South and Central America to the United States of America, the islands of the South Pacific, countries along the Atlantic coast of the Americas and certain countries in Africa. In the 1980s, research conducted by American and French teams enabled the breeding of stocks of spawners in captivity. This resulted in the establishment of lines of captive broodstock outside natural areas of distribution, and the development of new breeding farms in previously unproductive regions. Trade related to movements of these species is facilitated by the absence of legislation in most countries; even when such legislation does exist, it is not always applied These practices have led to the rapid development of world shrimp production, but have also contributed to the dissemination of pathogens. In recent years, the occurrence of serious epizootics - occasioning heavy losses world-wide - has led to a more cautious approach involving trade of post-larvae obtained from captive broodstock in which thorough control measures have been implemented for known diseases. Trade in wild shrimp will probably be abandoned progressively, to avoid the risk of introducing new pathogens. The main challenges for research in the near future are the development of diagnostic tools, to enable continuous control of captive broodstock, and the selection of strains which are resistant to the principal pathogens affecting these species. Revue Scientifique Et Technique-office International Des Epizooties (0253-1933) (Office Int Epizooties), 1996-06 , Vol. 15 , N. 2 , P. 499-515 Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/00091/20231/17883.pdf http://archimer.ifremer.fr/doc/00091/20231/ | Partager |
Shrimp aquaculture in New Caledonia Auteur(s) : Galinie, C Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 5, pp.45-50 Résumé : In New Caledonia, there is no indigenous shrimp species for commercial aquaculture and it is necessary to control the complete cycle in captivity. Since 1973 and the creation of the Station d'Aquaculture de Saint-Vincent (S.A.S.V.), a joint project between IFREMER, France-Aquaculture and the territory of New Caledonia, nine species have been tested and for one of these, P. stylirostris , (introduced in 1980), we are now in a commercial scale production with the tenth generation obtained in captivity. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1989/acte-1496.pdf http://archimer.ifremer.fr/doc/00000/1496/ | Partager Voir aussi New Caledonia Penaeus stylirostris Rearing Stocking density Larvae Growth Pellet feeds Intensive culture Télécharger |
Histological abnormalities related to mortality in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia Auteur(s) : Berthe, Franck Legroumellec, Marc Haffner, Philippe Renault, Tristan Éditeur(s) : European Association of Fish Pathologists Seventh International Conference Diseases of Fish and Shellfish Résumé : Outbreaks of mass mortality occurred since 1993 in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia. Since then, the disease, called "syndrome 93" (S93) has inflicted serious losses (60 to 100%) during the cold period and thus must be considered as an economically significant disease. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1995/acte-2974.pdf http://archimer.ifremer.fr/doc/00000/2974/ | Partager |
The state of the art of IFREMER in tropical aquaculture Auteur(s) : Calvas, Jacques Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 1, pp.3-9 Résumé : IFREMER, a French governmental agency, is engaged in research on tropical aquaculture since 1971, with the aims to develop new activities in French overseas territories and to export French technology to foreign countries. With 80 searchers and technicians the "Centre Oceanologique du Pacifique" is the main IFREMER's centre involved in tropical aquaculture. The research has been focused on the complete control of the whole cycle: constitution of broodstock, maturation and reproduction, larval rearing, and grow-out. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1989/acte-1500.pdf http://archimer.ifremer.fr/doc/00000/1500/ | Partager |
Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris Auteur(s) : Castex, Mathieu Lemaire, Pierrette Wabete, Nelly Chim, Liet Éditeur(s) : Elsevier Résumé : in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at a period of increased risk for an outbreak of the summer syndrome, higher mortalities were recorded, associated with high V. nigripulchritudo prevalence and loads in the shrimp haemolymph. In healthy shrimps (trial 1), no detectable response of the antioxidant defence system and of oxidative stress bio-indicators were observed. However, feeding the probiotic significantly increased the total antioxidant status (TAS) and glutathione peroxidase activity, while all other parameters remained significantly unchanged. In the shrimps exposed to V nigripulchritudo (trial 2), the antioxidant response was characterized by higher antioxidant enzyme activities (superoxide dismutase and catalase) and higher oxidative stress level in the digestive gland (higher oxidized/reduced glutathione ratio, higher malonclialdehyde and carbonyl protein contents) compared to levels found in trial 1. However, shrimps fed the probiotic diet exhibited (i) significantly higher final survival rates (67 +/- 3%) compared to the control (47 +/- 4%) and (ii) a lower prevalence of V nigripulchritudo throughout the trial. Moreover, the antioxidant response and the oxidative stress level recorded in the digestive gland with shrimps submitted to the probiotic diet were lower. In the light of these results, we confirm that P acidilactici enrichment in shrimps' diet seems an effective way of reducing the susceptibility of shrimps to bacterial pathogens. (c) 2009 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2009-09 , Vol. 294 , N. 3-4 , P. 306-313 Droits : 2009 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/2009/publication-6780.pdf DOI:10.1016/j.aquaculture.2009.06.016 http://archimer.ifremer.fr/doc/00000/6780/ | Partager |
Effets des renouvellements en eau sur l'écosystème des bassins d'élevages semi-intensifs de crevettes en Nouvelle-Calédonie durant la saison froide Auteur(s) : Lemonnier, Hugues Brizard, Raphael Résumé : Une expérience a été réalisée en conditions hivernales dans six bassins de terre pour déterminer l'effet des taux de renouvellement sur la production de crevettes Penaeus stylirostris, sur les caractéristiques de l'eau et du sédiment des enceintes d'élevage. Les budgets d'azote ont été établis en prenant en compte les différentes formes azotées dans l'eau, dans le sédiment, dans l'aliment et dans les crevettes. Un épisode de mortalité a été obserVé en cours d'élevage et peut être attribué à une epizootie dénommée "syndrome 93». Ce dernier était concomitant à une phase de croissance phytoplantonique et a été d'autant plus important que la biomasse phytoplanctonique dans le bassin d'élevage était élevée. Bien qu'une dilution importante de la colonne d'eau se soit avérée bénéfique pour la santé du cheptel pendant l'épisode de mortalité, l'augmentation des taux de renouvellement n'a en revanche pas permis d'améliorer de manière significative la qualité de l'environnement crevette sur toute la saison d'élevage (eau et sédiment). Une augmentation des échanges d'eau ne s'est pas accompagnée d'une meilleure exportation des déchets par les effluents. Cependant, les résultats montrent que cette augmentation accroît la productivité primaire. Les composés issus de la minéralisation de la matière organique et du métabolisme sont ainsi exportés sous forme particulaire plutôt que sous forme soluble. Les budgets d'azote montrent que la quantité de déchets rejetés par les effluents vers l'environnement côtier est faible. Ainsi, 10,1% à 16,90/o de l'azote introduit dans le milieu d'élevage sous forme d'aliment est exporté dans les effluents. Seulement 17,3% à 21,4% de l'azote d'origine alimentaire est retrouvé dans la chair des crevettes. L'augmentation des échanges d'eau implique une augmentation de l'accumulation azotée dans les sédiments de 21,0% à 50,1%. Parallèlement, le calcul de la proportion d'azote évacué par diffusion ou par dénitrification vers l'atmosphère diminue de 43,1% à 19,5% avec l'augmentation des taux de renouvellement. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/00079/19066/16661.pdf http://archimer.ifremer.fr/doc/00079/19066/ | Partager |