Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This study investigated the clinical epidemiology and resistance mechanisms of Acinetobacter baumannii and characterised the clonal diversity of carbapenem-resistant A. baumannii (CRAB) during an ICU-associated outbreak at Cayenne Hospital, French Guiana. All non-duplicate A. baumannii isolates from 2008 to 2014 were tested for antibiotic susceptibility by disk diffusion. Multilocus sequence typing, pulsed-field gel electrophoresis (PFGE) and characterisation of carbapenemase-encoding genes were performed on CRAB. Of the 441 A. baumannii isolates, most were from males (54.0%) and were detected mainly from the ICU (30.8%) and medicine wards (21.8%). In the ICU, strains were mainly isolated from the respiratory tract (44.1%) and bloodstream (14.0%), whereas in medicine wards they mainly were from wound/drainage (36.5%) and bloodstream (25.0%). A. baumannii showed the greatest susceptibility to piperacillin/tazobactam (92.7%), imipenem (92.5%), colistin (95.6%) and amikacin (97.2%), being lower in the ICU and medicine wards compared with other wards. An outbreak of OXA-23-producing CRAB occurred in the 13-bed ICU in 2010. CRAB strains were more co-resistant to other antimicrobials compared with non-CRAB. Molecular genetics analysis revealed five sequence types [ST78, ST107 and ST642 and two new STs (ST830 and ST831)]. Analysis of PFGE profiles indicated cross-transmissions of CRAB within the ICU, between the ICU and one medicine ward during transfer of patients, and within that medicine ward. This study provides the first clinical and molecular data of A. baumannii from French Guiana and the Amazon basin. The ICU was the highest risk unit of this nosocomial outbreak of OXA-23-producing CRAB, which could subsequently disseminate within the hospital.
ISSN: 0924-8579

hal-01506828
https://hal.archives-ouvertes.fr/hal-01506828
PUBMED : 27236843

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background - Pregnant women are a vulnerable population. Although regular follow-ups are recommended during pregnancy, not all pregnant women seek care. This pilot study wanted to assess whether the integration of data from administrative health information systems and socio-economic features allows identifying disparities in prenatal care trajectories.Methods - Prenatal care trajectories were extracted from the permanent sample of the French health insurance information system linked to the hospital discharge information system. The records of 2518 women who gave birth without complications in France in 2009 were analyzed. State sequence data analysis was performed to identify homogeneous groups of prenatal care trajectories. Socio-economic data were used to characterize their living environment.Results -We identified three groups of homogeneous prenatal care trajectories: (i) women with relatively high prenatal care consumption (~11 %), (ii) women with no prenatal care (~21 %), and (iii) women with an intermediate level of prenatal care (~66 %). Analysis of the socio-economic data demonstrated the association between disparities in prenatal care trajectories and the women’s living environment. Women with relatively high care consumption generally lived in socio-economically privileged areas (better education levels, employment status and housing conditions) compared with women with few or no prenatal care.Conclusions -Although ecological, our approach demonstrates that data from health administrative information systems could be used to describe prenatal care. However, more individual variables and an improvement of the data quality are needed to efficiently monitor the content and timing of prenatal care. Moreover, state sequence analysis, which was used in this context for the first time, proves to be an interesting approach to explore care trajectories. Finally, the integration of heterogeneous sources of data, including contextual information, might help identifying areas that require health promotion actions toward vulnerable populations, such as pregnant women.
ISSN: 1472-6963

hal-01169792
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01169792
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01169792/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01169792/file/Mining%20care%20trajectories%20using%20health%20administrative.pdf
DOI : 10.1186/s12913-015-0857-5

Éditeur(s) : HAL CCSD
Résumé : Carbon and Oxygen isotope profiles from a well-dated carbonate section spanning the Sinemurian time on the Caussenard High (southeast France) are compared to sedimentological data and the results from sequence stratigraphy analysis, in order to discuss the origin of isotopic variations in a shallow-marine carbonate succession. The sedimentological study has enabled the identification of four types of depositional environments (peritidal, lagoonal, sand shoal, outer-shelf) that formed meter-scale cycles. The latter are arranged in two third-order cycles whose boundaries correspond to distinct δ13C and δ18O negative peaks (at the end of the Hettangian time and during Middle Sinemurian). The origin of the isotopic signals is discussed and several hypothesis are envisaged: sea water ageing, early meteoric diagenesis. Isotopic curves observed here seems to be related to early meteoric diagenesis linked to variations of third-order sea-level cycles rather than environmental factors (temperature, salinity).
Swiss Journal of Geosciences

hal-00406603
https://hal.archives-ouvertes.fr/hal-00406603
DOI : 10.1007/s00015-007-1206-4

Éditeur(s) : HAL CCSD University of the West Indies
Résumé : International audience
La séquence de l'occupation précolombienne de l'île de Saint-Martin couvre une fourchette chronologique d'environ 4000 ans, de 2500 av. J.C. à 1500 ap.J.C.. On perçoit trois principales phases culturelles : une période précéramique suivie de deux phases céramiques, saladoïde et postsaladoïde. Deux référentiels chronologiques sont en cours d'élaboration. Le premier concerne l'occupation humaine restituée à partir de l'analyse de gisements précolombiens récemment documentés. Le second, qui se rapporte à l'évolution des paléomilieux, est construit à partir d'une analyse pluridisciplinaire du remplissage sédimentaire de plusieurs lagunes. La séquence étudiée a fourni à la base une datation vers 2900 av. J.C., elle couvre toute la période précolombienne. Les premiers résultats montrent des possibilités de corrélations entre les phases de transitions culturelles et les changements paléoclimatiques durant l'Holocène.
Proceedings of the twenty-fisrt congress of the international association for caribbean archaeology
Saint-Augustine, United States

halshs-00995384
https://halshs.archives-ouvertes.fr/halshs-00995384
https://halshs.archives-ouvertes.fr/halshs-00995384/document
https://halshs.archives-ouvertes.fr/halshs-00995384/file/Bonnissent_al_2007_PCR_aiac_cor.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The barrier reef tract of New Caledonia is the second largest in the world enclosing lagoons of variable width. The Late Quaternary evolution of these barrier reefs and lagoons is poorly known. A recent high resolution seismic and multibeam bathymetry survey was carried out in the south-western lagoon from shoreline to open ocean across the barrier reef pass systems to perform sequence stratigraphy and to better understand the Quaternary evolution of the lagoon. Two depositional sequences bounded by type-1 erosional surfaces can be identified. The bounding surfaces delineate the bottom of two generations of incised valleys. System tracts comprising a complete succession of regressive, lowstand, transgressive and highstand tracts were observed only in the upper sequence. Chronostratigraphic and sedimentological interpretations of the seismic data are based on correlations with the chronology defined in the nearby cores drilled through the reefs and in the gravity cores previously recovered in the inner lagoon. Interpretation of seismic data is also carried out on the basis of correlation with multibeam bathymetric data. Our results indicate that the history of the New Caledonian lagoon probably does not exceed 200ka marked by two or three brief episodes each of immersion of about ten thousand years. The bathymetric data reveal that during successive low sea level stands, the lagoon was dependent upon erosional fluvial networks. The deep fluvial valleys were connected to the continental slope through the passes. Seismic records reveal that upstream palaeo-channels are not connected to the modern coastal rivers. In contrast, many aggrading deposits, including numerous stacking channels, and extending across the proximal part of the outer lagoon, can be identified. Examination of drainage network morphology suggests a progressive tilt of the outer lagoon. Such evolution reveals that the Nouméa lagoon which is one of the widest in the world is an exceptional modern example of a rimmed shelf providing data to analyze the past counterparts.
ISSN: 0031-0182

insu-00338953
https://hal-insu.archives-ouvertes.fr/insu-00338953
DOI : 10.1016/j.palaeo.2008.08.012

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: The filamentous fungus Trichoderma reesei is the main industrial cellulolytic enzyme producer. Several strains have been developed in the past using random mutagenesis, and despite impressive performance enhancements, the pressure for low-cost cellulases has stimulated continuous research in the field. In this context, comparative study of the lower and higher producer strains obtained through random mutagenesis using systems biology tools (genome and transcriptome sequencing) can shed light on the mechanisms of cellulase production and help identify genes linked to performance. Previously, our group published comparative genome sequencing of the lower and higher producer strains NG 14 and RUT C30. In this follow-up work, we examine how these mutations affect phenotype as regards the transcriptome and cultivation behaviour. Results: We performed kinetic transcriptome analysis of the NG 14 and RUT C30 strains of early enzyme production induced by lactose using bioreactor cultivations close to an industrial cultivation regime. RUT C30 exhibited both earlier onset of protein production (3 h) and higher steady-state productivity. A rather small number of genes compared to previous studies were regulated (568), most of them being specific to the NG 14 strain (319). Clustering analysis highlighted similar behaviour for some functional categories and allowed us to distinguish between induction-related genes and productivity-related genes. Cross-comparison of our transcriptome data with previously identified mutations revealed that most genes from our dataset have not been mutated. Interestingly, the few mutated genes belong to the same clusters, suggesting that these clusters contain genes playing a role in strain performance. Conclusions: This is the first kinetic analysis of a transcriptomic study carried out under conditions approaching industrial ones with two related strains of T. reesei showing distinctive cultivation behaviour. Our study sheds some light on some of the events occurring in these strains following induction by lactose. The fact that few regulated genes have been affected by mutagenesis suggests that the induction mechanism is essentially intact compared to that for the wild-type isolate QM6a and might be engineered for further improvement of T. reesei. Genes from two specific clusters might be potential targets for such genetic engineering.
ISSN: 1754-6834

Droits : http://creativecommons.org/licenses/by/
hal-01112360
http://hal.upmc.fr/hal-01112360
http://hal.upmc.fr/hal-01112360/document
http://hal.upmc.fr/hal-01112360/file/Poggi-Parodi_2014_Kinetic.pdf
DOI : 10.1186/s13068-014-0173-z

Éditeur(s) : HAL CCSD Elsevier
Résumé : Rh (Rhesus) is a major blood group system in man, which is clinically significant in transfusion medicine. Rh antigens are carried by an oligomer of two major erythroid specific polypeptides, the Rh (D and CcEe) proteins and the RhAG glycoprotein, that shared a common predicted structure with 12 transmembrane a-helices (M0 to M11). Non erythroid homologues of these proteins have been identified (RhBG and RhCG), notably in diverse organs specialized in ammonia production and excretion, such as kidney, liver and intestine. Phylogenetic studies and experimental evidence have shown that these proteins belong to the Amt/Mep/Rh protein superfamily of ammonium/methylammonium permease, but another view suggests that Rh proteins might function as CO2 gas channels. Until recently no information on the structure of these proteins were available. However, in the last two years, new insight has been gained into the structural features of Rh proteins (through the determination of the crystal structures of bacterial AmtB and archeaebacterial Amt-1. Here, models of the subunit and oligomeric architecture of human Rh proteins are proposed, based on a refined alignment with and crystal structure of the bacterial ammonia transporter AmtB, a member of the Amt/ Mep/Rh superfamily. This alignment was performed considering invariant structural features, which were revealed through Hydrophobic Cluster Analysis, and led to propose alternative predictions for the less conserved regions, particularly in the N-terminal sequences. The Rh models, on which an additional Rh-specific, N-terminal helix M0 was tentatively positioned, were further assessed through the consideration of biochemical and immunochemical data, as well as of stereochemical and topological constraints. These models highlighted some Rh specific features that have not yet been reported. Among these, are the prediction of some critical residues, which may play a role in the channel function, but also in the stability of the subunit structure and oligomeric assembly. These results provide a basis to further understand the structure/function relationships of Rh proteins, and the alterations occurring in variant phenotypes.
ISSN: 1246-7820

hal-00068842
https://hal.archives-ouvertes.fr/hal-00068842

Éditeur(s) : HAL CCSD
Résumé : International audience
Calculation of seismic risks in a zone are based on stochastic models describing at best the seismic activity. Our focus is the spatio-temporal evolution of the probability of earthquake occurrences. The main modelling tool is the theory of the point processes. We discuss about the spatio-temporal extension of ETAS (Epidemic Type Aftershock Sequence) with marks and study the properties of the log-likelihood and the maximum likelihood estimators. Seismic data from the Lesser Antilles Arc carried out between 1999 and 2004 are analyzed.
39th Annual Meeting of the Statistical Society of Canada
Wolfville, Canada

hal-00601513
https://hal.archives-ouvertes.fr/hal-00601513

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Rh (Rhesus) is a major blood group system in man, which is clinically significant in transfusion medicine. Rh antigens are carried by an oligomer of two major erythroid specific polypeptides, the Rh (D and CcEe) proteins and the RhAG glycoprotein, that shared a common predicted structure with 12 transmembrane a-helices (M0 to M11). Non erythroid homologues of these proteins have been identified (RhBG and RhCG), notably in diverse organs specialized in ammonia production and excretion, such as kidney, liver and intestine. Phylogenetic studies and experimental evidence have shown that these proteins belong to the Amt/Mep/Rh protein superfamily of ammonium/methylammonium permease, but another view suggests that Rh proteins might function as CO2 gas channels. Until recently no information on the structure of these proteins were available. However, in the last two years, new insight has been gained into the structural features of Rh proteins (through the determination of the crystal structures of bacterial AmtB and archeaebacterial Amt-1. Here, models of the subunit and oligomeric architecture of human Rh proteins are proposed, based on a refined alignment with and crystal structure of the bacterial ammonia transporter AmtB, a member of the Amt/Mep/Rh superfamily. This alignment was performed considering invariant structural features, which were revealed through Hydrophobic Cluster Analysis, and led to propose alternative predictions for the less conserved regions, particularly in the N-terminal sequences. The Rh models, on which an additional Rh-specific, N-terminal helix M0 was tentatively positioned, were further assessed through the consideration of biochemical and immunochemical data, as well as of stereochemical and topological constraints. These models highlighted some Rh specific features that have not yet been reported. Among these, are the prediction of some critical residues, which may play a role in the channel function, but also in the stability of the subunit structure and oligomeric assembly. These results provide a basis to further understand the structure/function relationships of Rh proteins, and the alterations occurring in variant phenotypes.
ISSN: 1246-7820

hal-00112914
https://hal.archives-ouvertes.fr/hal-00112914
DOI : 10.1016/j.tracli.2006.02.001