Éditeur(s) : Mdpi Ag
Résumé : Marine pelagic diazotrophic cyanobacteria of the genus Trichodesmium (Oscillatoriales) are widespread throughout the tropics and subtropics, and are particularly common in the waters of New Caledonia. Blooms of Trichodesmium are suspected to be a potential source of toxins in the ciguatera food chain and were previously reported to contain several types of paralyzing toxins. The toxicity of water-soluble extracts of Trichodesmium spp. were analyzed by mouse bioassay and Neuroblastoma assay and their toxic compounds characterized using liquid chromatography coupled with tandem mass spectrometry techniques. Here, we report the first identification of palytoxin and one of its derivatives, 42-hydroxy-palytoxin, in field samples of Trichodesmium collected in the New Caledonian lagoon. The possible role played by Trichodesmium blooms in the development of clupeotoxism, this human intoxication following the ingestion of plankton-eating fish and classically associated with Ostreopsis blooms, is also discussed.
Marine Drugs (1660-3397) (Mdpi Ag), 2011-04 , Vol. 9 , N. 4 , P. 543-560

Droits : 2011 MDPI Publishing
http://archimer.ifremer.fr/doc/00040/15143/12576.pdf
DOI:10.3390/md9040543
http://archimer.ifremer.fr/doc/00040/15143/

Éditeur(s) : HAL CCSD
Résumé : Satellite and in situ chlorophyll concentration data collected as part of VALidation HYperspectral of a BIOgeochemical model (ValHyBio), a PNTS-sponsored project dedicated to satellite ocean-colour imaging of the Southwest Pacific, are analyzed to describe chlorophyll variability in the Coral Sea, a vast oligotrophic region characterized by a deep chlorophyll maximum and blue waters of high transparency. Average chlorophyll concentrations estimated from MODIS-Aqua are very low (<0.2 mg m-3) except in the vicinity of main islands and coral reefs, where enrichment may occur due to upwelling or internal waves that bring nutrients to the euphotic zone from the deep part of the water column. During the summer season, blooms of cyanobacteria (Trichodesmium) develop in the surface waters and may form large slicks. The nitrogen fixed by these slicks is a potential source of new nitrogen later assimilated by picoplankton and the marine food chain. Coastal areas exhibit higher chlorophyll concentrations because of nutrient input from the land, as observed in 2008 around New Caledonia. In lagoon areas, the sea colour is influenced by turbidity and bathymetry, and the MODIS OC3 algorithm is inadequate, with a systematic overestimation of chlorophyll concentration in the New Caledonia lagoon. Improved bio-optical algorithms are needed for those turbid and shallow waters, to allow not only a better description of chlorophyll variability, but also evaluation of chlorophyll simulations by recently developed biogeochemical models.
of the 12th International Coral Reef Symposium, 18E
CAIRNS, Australia

hal-00746470
https://hal.archives-ouvertes.fr/hal-00746470
https://hal.archives-ouvertes.fr/hal-00746470/document

Éditeur(s) : Copernicus Gesellschaft Mbh
Résumé : The Mediterranean Sea is one of the most oligotrophic marine areas on earth where nitrogen fixation has formally believed to play an important role in carbon and nitrogen fluxes. Although this view is under debate, the diazotrophs responsible for this activity have still not been investigated in the open sea. In this study, we characterised the surface distribution and species richness of unicellular and filamentous diazotrophs across the Mediterranean Sea by combining microscopic counts with size fractionated in situ hybridization (TSA-FISH), and 16S rDNA and nifH genes phylogenies. These genetic analyses were possible owing to the development of a new PCR protocol adapted to scarce microorganisms that can detect as few as 1 cell ml(-1) in cultures. Low concentrations of diazotrophic cyanobacteria were detected and this community was dominated at 99.9% by picoplankton hybridized to the Nitro821 probe, specific for unicellular diazotrophic cyanobacteria (UCYN). Among filamentous cyanobacteria only 0.02 filament ml(-1) of Richelia were detected in the eastern basin, while small (0.7-1.5 mu m) and large (2.5-3.2 mu m) Nitro821-targeted cells were recovered at all stations with a mean concentration of 3.5 cell ml(-1). The affiliation of the small Nitro821-targeted cells to UCYN-A was confirmed by 16S and nifH phylogenies in the western Mediterranean Sea. In the central and the eastern Mediterranean Sea no 16S rDNA and nifH sequence from UCYN was obtained as cells concentration were close to, or below PCR detection limit. Bradyrhizobium sequences dominated nifH clone libraries from picoplanktonic size fractions. A few sequences of gamma-proteobacteria were also detected in the central Mediterranean Sea. While low phosphate and iron concentrations could explain the absence of Trichodesmium sp., the factors that prevent the development of UCYN-B and C remain unknown. We also propose that the dominating picoplankters probably developed specific strategies, such as associations with protists or particles, and/or photosynthetic activity, to acquire carbon for sustaining diazotrophy.
Biogeosciences (1726-4170) (Copernicus Gesellschaft Mbh), 2011 , Vol. 8 , N. 3 , P. 827-840

Droits : Author(s) 2011. CC Attribution 3.0 License.
http://archimer.ifremer.fr/doc/00133/24392/22416.pdf
DOI:10.5194/bg-8-827-2011
http://archimer.ifremer.fr/doc/00133/24392/