Éditeur(s) : HAL CCSD
Résumé : National audience
Congrès de la SPTC - Société de Pharmaco-Toxicologie Cellulaire
Paris, France

hal-01122677
https://hal.archives-ouvertes.fr/hal-01122677

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Souttoufide belt is situated between the High-Atlas in the north, the Mauritanides in the south and the West African Craton as well as the Tindouf basin in the east. Thus, this belt is a part of the West African fold belt system which surrounds the West African Craton. Outcrops occur only in three areas, while the rest of the Souttoufide belt is covered by Meso- to Cenozoic sediments. These areas are, from the south to the north, the Adrar Souttouf Massif, the Smara Zemmour area, and the western part of the Anti-Atlas. Because of the continuing processing and numerous publications, the Anti-Atlas cannot be dealt with in detail. Fundamental geological work in both of the other areas was mainly done during the second third of the 20th century, followed by several decades of inactivity. Numerous studies were done in the Adrar Souttouf massif during the last decade, while the Smara-Zemmour area still remains poorly investigated. Most of them were focused on geochronological analyses and geochemistry. Accordingly, the obtained data led to a new interpretation. The Adrar Souttouf is interpreted as a Pan-African belt exhibiting a Neoproterozoic island arc reworked by the Variscan collision between the North American Craton (NAC) and the West African Craton (WAC). The Smara-Zemmour area is considered as the external part of this Variscan belt thrusted onto the Palaeozoic Tindouf basin. The western Anti-Atlas as considered as a Pan-African active margin reworked during the Variscan orogen. Together, these three areas, lead to the hypothesis of a Late Pan-African belt capped by the Early and Middle Palaeozoic covers and then affected by the Middle Carboniferous tectonic event (330 Ma) related to the Pangaean assembly. Correlations with adjacent belts (Mauritanides and Anti-Atlas) are considered.
ISSN: 0899-5362

hal-01382815
https://hal.archives-ouvertes.fr/hal-01382815
DOI : 10.1016/j.jafrearsci.2015.04.016

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Toxicology Letters
Paris, France

hal-01122721
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122721
DOI : 10.1016/j.toxlet.2011.05.182

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Heterocyclic aromatic amines (HAA) are formed in cooked meat, poultry and fish but also arise in tobacco smoke and exhaust gases. HAA are potential human carcinogens, which require metabolic activation to exert their genotoxicity. Human tissues can bioactivate HAA to produce reactive intermediates that bind to DNA. HAA DNA adduct formation occurs in human hepatocytes; however, the potential of HAA to form DNA adducts has not been investigated in human T lymphocytes. In this study, we investigated the ability of human T lymphocytes activated with PMA/Ionomycin or CD3/CD28 to express functional CYP1 activity and bioactivate three major HAA: 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), and 2-amino-9H-pyrido[2,3-b]indole (AαC) to form DNA adducts. Adducts were measured by ultraperformance liquid chromatography-electrospray ionization/multistage scan mass spectrometry. The highest level of DNA adducts occurred for AαC (16 adducts per 109 nucleotides), followed by PhIP (9 adducts per 109 nucleotides). In contrast, DNA adducts formed from MeIQx and the structurally related aromatic amine 4-aminobiphenyl, a known human carcinogen, were below the limit of detection (< 3 adducts per 109 nucleotides). Moreover, we demonstrate that AαC is a potent inducer of CYP1A1 and CYP1B1 activity through a transcriptional mechanism involving the AhR pathway. Overall, our results highlight the capacity of activated human T lymphocytes to more efficiently bioactivate AαC to form DNA adducts than other prominent HAA or 4-ABP. Environ. Mol. Mutagen. 57:656-667, 2016.
ISSN: 0893-6692

hal-01400486
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01400486
DOI : 10.1002/em.22059
PUBMED : 27801952

Éditeur(s) : HAL CCSD
Résumé : International audience
BACKGROUND: Dairy working increases the prevalence of lower airway respiratory diseases, especially COPD and asthma. Epidemiological studies have reported that chronic inhalation of organic dusts released during specific daily tasks could represent a major risk factor for development of these pathologies in dairy workers. Knowledge on size, nature and biological activity of such organic dusts remain however limited. OBJECTIVE: To compare size distribution, microbial composition and cellular effects of dusts liberated by the spreading of straw bedding in five French dairy farms located in Brittany. RESULTS: Mechanized distribution of straw bedding generated a cloud of inhalable dusts in the five dairy farms' barns. Thoracic particles having a 3-7.5µm size constituted 58.9-68.3% of these dusts. Analyses of thoracic dusts by next generation sequencing showed that the microbial dust composition differed between the five French farms, although Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria represent more than 97.5% of the bacterial phyla detected in each sample. Several bacteria genera comprising of human pathogenic species, such as Pseudomonas, Staphylococcus, Thermoactinomyces or Saccharopolyspora were identified. Cladosporium and Alternaria fungal genera, which are potent environmental determinants of respiratory symptoms, were detected in dusts collected in the five farms and their levels reached 15.5-51.1% and 9-24.7% of assignable fungal sequences in each sample, respectively. Finally, all dust samples significantly and strongly increased the expression of the pro-inflammatory TNF-α, IL-1β, IL-6 and IL-8 cytokines at both mRNA and protein levels in human monocyte-derived macrophages. Their effects were dose-dependent and detectable from 1µg/ml. The intensity of the macrophage responses however differed according to the samples. CONCLUSIONS: Our results strengthen the hypothesis that organic dusts released during the distribution of straw bedding are mainly constituted of thoracic particles which are small enough to deposit on lower bronchial epithelium of dairy farmers and induce inflammation.
Environmental Research

hal-01579610
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01579610
DOI : 10.1016/j.envres.2017.07.025
PUBMED : 28735230