Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
: Previously, we demonstrated that eicosapentaenoic acid enhanced ethanol-induced oxidative stress and cell death in primary rat hepatocytes via an increase in membrane fluidity and lipid raft clustering. In this context, another n-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA), was tested with a special emphasis on physical and chemical alteration of lipid rafts. Pretreatment of hepatocytes with DHA reduced significantly ethanol-induced oxidative stress and cell death. DHA protection could be related to an alteration of lipid rafts. Indeed, rafts exhibited a marked increase in membrane fluidity and packing defects leading to the exclusion of a raft protein marker, flotillin. Furthermore, DHA strongly inhibited disulfide bridge formation, even in control cells, thus suggesting a disruption of protein-protein interactions inside lipid rafts. This particular spatial organization of lipid rafts due to DHA subsequently prevented the ethanol-induced lipid raft clustering. Such a prevention was then responsible for the inhibition of phospholipase C-γ translocation into rafts, and consequently of both lysosome accumulation and elevation in cellular low-molecular-weight iron content, a prooxidant factor. In total, the present study suggests that DHA supplementation could represent a new preventive approach for patients with alcoholic liver disease based upon modulation of the membrane structures.
ISSN: 0278-6915

hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/file/Sergent-et-al-incl-figures-accepted.pdf
DOI : 10.1016/j.fct.2013.07.061

Éditeur(s) : CRILLASH : Centre de Recherches Interdisciplinaires en Lettres, Langues, Arts et Sciences Humaines
Extrait de : "Poétique et politique de l'altérité : colonialisme, esclavagisme, exotisme dans la littérature et les arts (XVIIe-XXIe siècles)" : colloque international, le 12 mars 2015. Université des Antilles et de la Guyane
Description : A partir du présupposé qui considère que les clichés idéalisant les Antilles ou, à l'inverse, les dévalorisant, continuent d'innerver les imaginaires européens et caribéens, une recherche a été développée sur le rapport du corps à ces clichés et ces préjugés. Qu'est-ce qu'une vision stéréotypée transposée dans le temps, l'espace ? Quel est l'impact du stéréotype sur les corps, leur mouvement, leur inscription dans le temps, dans l'espace et la musique ?
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15158
V15158

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
An active seismic experiment has been conducted across the southern Ryukyu margin east of Taiwan over the whole trench-arc-backarc system in May 2009. Twenty-four ocean bottom seismometers (OBS) were deployed from the Ryukyu trench to the southern Okinawa trough over the Ryukyu arc and forearc. Wide angle seismic data were recorded by the OBS array while coincident reflection seismic data were acquired using a 6 km long streamer and a 6600 cubic inch seismic airgun array. Results from tomographic inversion of 21091 travel time picks along this line allowed us to image crustal structures of the Ryukyu margin down to a depth of 25 km. The transect has been designed to provide a better seismic velocity structure of the subduction zone in a highly deformed area that has produced an M8 earthquake in 1920. The line crosses a seismic cluster of earthquakes which source mechanisms are still poorly understood. The subducting oceanic crust of the Huatung Basin is about 5-6 km thick. The underlying mantle exhibits low seismic velocities around 7.8 km/s suggesting some hydrothermal alterations or alteration of the upper mantle through faults generated by the flexure of the subducting plate as it enters the subduction. Low velocities, up to 4.5 km/s, associated with the accretionary wedge are well imaged from the trench back to the Nanao forearc. A major result concerns the abrupt termination of the buttress at the rear of the accretionary wedge. Despite the low resolution of the tomographic inversion near the subduction interface, several lines of evidence supporting the presence of a low velocity zone beneath the toe of the forearc buttress could be established. The Moho beneath the Ryukyu non-volcanic arc is located at a depth around 25 km depth.
ISSN: 0040-1951

hal-00795573
https://hal.archives-ouvertes.fr/hal-00795573
DOI : 10.1016/j.tecto.2011.10.010

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Expeditions 304 and 305 of the Integrated Ocean Drilling Program cored and logged a 1.4 km section of the domal core of Atlantis Massif. Postdrilling research results summarized here constrain the structure and lithology of the Central Dome of this oceanic core complex. The dominantly gabbroic sequence recovered contrasts with predrilling predictions; application of the ground truth in subsequent geophysical processing has produced self-consistent models for the Central Dome. The presence of many thin interfingered petrologic units indicates that the intrusions forming the domal core were emplaced over a minimum of 100-220 kyr, and not as a single magma pulse. Isotopic and mineralogical alteration is intense in the upper 100 m but decreases in intensity with depth. Below 800 m, alteration is restricted to narrow zones surrounding faults, veins, igneous contacts, and to an interval of locally intense serpentinization in olivine-rich troctolite. Hydration of the lithosphere occurred over the complete range of temperature conditions from granulite to zeolite facies, but was predominantly in the amphibolite and greenschist range. Deformation of the sequence was remarkably localized, despite paleomagnetic indications that the dome has undergone at least 45 degrees rotation, presumably during unroofing via detachment faulting. Both the deformation pattern and the lithology contrast with what is known from seafloor studies on the adjacent Southern Ridge of the massif. There, the detachment capping the domal core deformed a 100 m thick zone and serpentinized peridotite comprises similar to 70% of recovered samples. We develop a working model of the evolution of Atlantis Massif over the past 2 Myr, outlining several stages that could explain the observed similarities and differences between the Central Dome and the Southern Ridge.
ISSN: 0148-0227

hal-00617528
https://hal.archives-ouvertes.fr/hal-00617528
https://hal.archives-ouvertes.fr/hal-00617528/document
https://hal.archives-ouvertes.fr/hal-00617528/file/2010JB007931.pdf
DOI : 10.1029/2010JB007931

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Cell proliferation is a hallmark of cancer and depends on complex signaling networks that are chiefly supported by protein kinase activities. Therapeutic strategies have been used to target specific kinases but new methods are required to identify combined targets and improve treatment. Here, we propose a small interfering RNA genetic screen and an integrative approach to identify kinase networks involved in the proliferation of cancer cells. The functional siRNA screen of 714 kinases in HeLa cells identified 91 kinases implicated in the regulation of cell growth, most of them never being reported in previous whole-genome siRNA screens. Based on gene ontology annotations, we have further discriminated between two classes of kinases that, when suppressed, result in alterations of the mitotic index and provoke cell-cycle arrest. Extinguished kinases that lead to a low mitotic index mostly include kinases implicated in cytosolic signaling. In contrast, extinguished kinases that result in a high mitotic index mostly include kinases implicated in cell division. By mapping hit kinases in the PhosphPOINT phosphoprotein database, we generated scale-free networks consisting of 449 and 661 protein-protein interactions for kinases from low MI and high MI groups, respectively. Further analyses of the kinase interactomes revealed specific modules such as FER- and CRKL-containing modules that connect three members of the epidermal growth factor receptor (EGFR) family, suggesting a tight control of the mitogenic EGF-dependent pathway. Based on experimental studies, we confirm the involvement of these two kinases in the regulation of tumor cell growth. Based on a combined approach of large kinome-wide siRNA screens and ontology annotations, our study identifies for the first time two kinase groups differentially implicated in the control of cell proliferation. We further demonstrate that integrative analysis of the kinase interactome provides key information which can be used to facilitate or optimize target design for new therapeutic strategies. The complete list of protein-protein interactions from the two functional kinase groups will provide a useful database for future investigations.
ISSN: 1471-2164

hal-01118475
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118475
DOI : 10.1186/1471-2164-15-1169
PUBMED : 25540073

Résumé : Once fished, the shrimps must be cooled quickly in water cooled in order to slow down all chemical and bacteriological activities. In addition, in order to avoid blackening (mélanose) shrimps (céphalothorax and legs) it is necessary to treat those with an antioxydant product. The product currently used to inhibit this enzymatic change is the soda niétabisuifite (Na-> S7 O5), until now, after being fished. the shrimps were directly soaked in a cooled water tank, containing the pesticide. The solution of treatment was progressively prepared needs. The concentration of this one was approximately 2 to 3% of métabisulfite, that is to say 6 to 9 G of SO2 per liter for a starting product containing 320 G of SO2 approximately. In order to preserve a good quality of the liquid of treatment it was essential to often renew it (every 400 to 500 kg) what was not always the case, the métabisulfite being often added progressively with the liking of the appointed person with the treatment of shrimps. In order to cure this delicate problem of renewal of vat and uniformity of the treatment, the SODACAL obtained the system of treatment by sprinkling.
Une fois pêchées, les crevettes doivent être refroidies rapidement dans de l'eau réfrigérée afin de ralentir toutes activités chimiques et bactériologiques. D'autre part, afin d'éviter le noircissement (mélanose) des crevettes (céphalothorax et pattes) il est nécessaire de traiter celles-ci avec un produit anti-oxydant. Le produit actuellement utilisé pour inhiber cette altération enzymatique est le niétabisuifite de soude (Na-> S7 O5), jusqu'à maintenant, après avoir été pêchées. les crevettes étaient directement trempées dans une cuve d'eau réfrigérée, contenant le produit de traitement. La solution de traitement était préparée au fur et à mesure des besoins. La concentration de celle-ci était d'environ 2 à 3 % de métabisulfite, soit 6 à 9 g de SO2 par litre pour un produit de départ contenant 320 g de SO9 environ. Afin de conserver une bonne qualité du liquide de traitement il était indispensable de le renouveler souvent (tous les 400 à 500 kg) ce qui n'était pas toujours le cas, le métabisulfite étant souvent rajouté au fur et à mesure au gré de la personne préposée au traitement des crevettes. Afin de remédier à ce problème délicat de renouvellement de bac et d'uniformité du traitement, la SODACAL s'est dotée du système de traitement par aspersion.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1991/rapport-1648.pdf
http://archimer.ifremer.fr/doc/00000/1648/

Éditeur(s) : CRILLASH : Centre de Recherches Interdisciplinaires en Lettres, Langues, Arts et Sciences Humaines
Extrait de : "Poétique et politique de l'altérité : colonialisme, esclavagisme, exotisme dans la littérature et les arts (XVIIe-XXIe siècles)" : colloque international, le 12 mars 2015. Université des Antilles et de la Guyane
Description : La musique martiniquaise est caractéristique à la fois d'une identité et d'ouvertures qui laissent apparaître une capacité à véhiculer ses marqueurs tandis qu'elle est le vecteur d'une valorisation des musiques nées de rencontres avec les cultures autres. La biguine martiniquaise, témoin depuis le 19e siècle d'une évolution signifiante des sociétés qui l'ont créée et qui en dépendent, résulte de la rencontre de peuples étrangers à la terre occupée, tandis qu'elle marque avec force les antagonismes de ces mêmes peuples.
Siècle(s) traité(s) : 19
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15164
V15164

Éditeur(s) : HAL CCSD BioScientifica
Résumé : International audience
Paracetamol/acetaminophen (N-Acetyl-p-Aminophenol; APAP) is the preferred analgesic for pain relief and fever during pregnancy. It has therefore caused concern that several studies have reported that prenatal exposure to APAP results in developmental alterations in both the reproductive tract and the brain. Genitals and nervous system of male mammals are actively masculinised during foetal development and early postnatal life by the combined actions of prostaglandins and androgens, resulting in the male-typical reproductive behaviour seen in adulthood. Both androgens and prostaglandins are known to be inhibited by APAP. Through intrauterine exposure experiments in C57BL/6 mice, we found that exposure to APAP decreased neuronal number in the sexually dimorphic nucleus (SDN) of the preoptic area (POA) in the anterior hypothalamus of male adult offspring. Likewise, exposure to the environmental pollutant and precursor of APAP, aniline, resulted in a similar reduction. Decrease in neuronal number in the SDN-POA is associated with reductions in male sexual behaviour. Consistent with the changes, male mice exposed in uteri to APAP exhibited changes in urinary marking behaviour as adults and had a less aggressive territorial display towards intruders of the same gender. Additionally, exposed males had reduced intromissions and ejaculations during mating with females in oestrus. Together, these data suggest that prenatal exposure to APAP may impair male sexual behaviour in adulthood by disrupting the sexual neurobehavioral programming. These findings add to the growing body of evidence suggesting the need to limit the widespread exposure and use of APAP by pregnant women.
ISSN: 1470-1626

hal-01560285
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285/file/Prenatal%20exposures%20to%20paracetamol-accepted.pdf
DOI : 10.1530/REP-17-0165

Éditeur(s) : HAL CCSD Techno-Press
Résumé : Fractures and faults riddle the Earth’s crust on all scales, and the deformation associated with them is presumed to have had significant effects on its petrological and structural evolution. However, despite the abundance of directly observable earthquake activity, unequivocal evidence for seismic slip rates along ancient faults is rare and usually related to frictional melting and the formation of pseudotachylites. We report novel microstructures from garnet crystals in the immediate vicinity of seismic slip planes that transected lower crustal granulites during intermediate-depth earthquakes in the Bergen Arcs area, western Norway, some 420 million years ago. Seismic loading caused massive dislocation formations and fragmentation of wall rock garnets. Microfracturing and the injection of sulfide melts occurred during an early stage of loading. Subsequent dilation caused pervasive transport of fluids into the garnets along a network of microfractures, dislocations, and subgrain and grain boundaries, leading to the growth of abundant mineral inclusions inside the fragmented garnets. Recrystallization by grain boundary migration closed most of the pores and fractures generated by the seismic event. This wall rock alteration represents the initial stages of an earthquake-triggered metamorphic transformation process that ultimately led to reworking of the lower crust on a regional scale.
ISSN: 2287-528X

hal-01553309
https://hal.archives-ouvertes.fr/hal-01553309
https://hal.archives-ouvertes.fr/hal-01553309/document
https://hal.archives-ouvertes.fr/hal-01553309/file/austrheim2017.pdf
DOI : 10.1126/sciadv.1602067

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
: We investigated the in vitro effects of four alkyl-galactofuranoside derivatives, i.e. octyl-β-D-galactofuranoside (1), 6-amino-β-D-galactofuranoside (2), 6-N-acetamido-β-D-galactofuranoside (3), and 6-azido-β-D-galactofuranoside (4) on Leishmania donovani. Their mechanism of action was explored using electron paramagnetic resonance spectroscopy (EPR) and nuclear magnetic resonance (NMR), and ultrastructural alterations by transmission electron microscopy (TEM). Compound 1 showed the most promising effects by inhibiting promastigote growth at IC50 8.96 ± 2.5 μM. All compounds exhibit low toxicity towards human macrophages. Compound 1 had a higher selectivity index than the comparative molecule used, i.e. miltefosine (159.7 versus 37.9, respectively). EPR showed that compound 1 significantly reduced membrane fluidity, compared to control promastigotes and to compound 3. The furanose ring was shown to support this effect since the isomer galactopyranose had no effect on parasite membrane fluidity or growth. NMR showed a direct interaction of all compounds (1>2>3>4) with the promastigote membrane, as well as with octyl-galactopyranose and octanol, providing evidence that the n-octyl chain was primarily involved in the anchoring with the parasite membrane, followed by the putative crucial role of furanose ring in the anti-leishmanial activity. A morphologic analysis of compound 1-treated promastigotes by TEM revealed profound alterations on parasite membrane and organelles, but not with compound 3. Quantification of annexinV binding by flow cytometry confirmed that compound 1 induced apoptosis in >90% promastigotes. The effect of compound 1 was also assessed on intra-macrophagic amastigotes, and showed a reduction in amastigote growth associated with an increase of ROS production, thus validating its promising effect.
ISSN: 0066-4804

hal-00941772
https://hal.archives-ouvertes.fr/hal-00941772
PUBMED : 24468785
DOI : 10.1128/AAC.01350-13

Éditeur(s) : HAL CCSD
Résumé : International audience
Two fluorescent galactofuranosides were synthesized and their biological activities evaluated on non-infected and Leishmania infected macrophages. Both tagged scaffolds were able to penetrate macrophages. Compared to the activity of the parent octyl galactofuranoside used as a reference, the fluorescein-conjugate showed altered biological properties while the rhodamine 6G one synergistically acted with the lipid chain to significantly increase antiparasitic activity.
Bioorganic & Medicinal Chemistry Letters

hal-01446721
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446721
PUBMED : 27956346
DOI : 10.1016/j.bmcl.2016.11.090

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Under physiological conditions, the liver sinusoidal endothelial cells (LSECs) mediate hepatic immune tolerance toward self or foreign antigens through constitutive expression of anti-inflammatory mediators. However, upon viral infection or Toll-like receptor 2 (TLR2) activation, LSECs can achieve proinflammatory functions, but their role in hepatic inflammation during acute viral hepatitis is unknown. Using the highly virulent mouse hepatitis virus type 3 (MHV3) and the attenuated variants 51.6-MHV3 and YAC-MHV3, exhibiting lower tropism for LSECs, we investigated in vivo and in vitro the consequence of LSEC infection on their proinflammatory profiles and the aggravation of acute hepatitis process. In vivo infection with virulent MHV3, in comparison to attenuated strains, resulted in fulminant hepatitis associated with higher hepatic viral load, tissue necrosis, and levels of inflammatory mediators and earlier recruitment of inflammatory cells. Such hepatic inflammatory disorders correlated with disturbed production of interleukin-10 (IL-10) and vascular factors by LSECs. We next showed in vitro that infection of LSECs by the virulent MHV3 strain altered their production of anti-inflammatory cytokines and promoted higher release of proinflammatory and procoagulant factors and earlier cell damage than infection by attenuated strains. This higher replication and proinflammatory activation in LSECs by the virulent MHV3 strain was associated with a specific activation of TLR2 signaling by the virus. We provide evidence that TLR2 activation of LSCEs by MHV3 is an aggravating factor of hepatic inflammation and correlates with the severity of hepatitis. Taken together, these results indicate that preservation of the immunotolerant properties of LSECs during acute viral hepatitis is imperative in order to limit hepatic inflammation and damage. IMPORTANCE Viral hepatitis B and C infections are serious health problems affecting over 350 million and 170 million people worldwide, respectively. It has been suggested that a balance between protection and liver damage mediated by the host's immune response during the acute phase of infection would be determinant in hepatitis outcome. Thus, it appears crucial to identify the factors that predispose in exacerbating liver inflammation to limit hepatocyte injury. Liver sinusoidal endothelial cells (LSECs) can express both anti- and proinflammatory functions, but their role in acute viral hepatitis has never been investigated. Using mouse hepatitis virus (MHV) infections as animal models of viral hepatitis, we report for the first time that in vitro and in vivo infection of LSECs by the pathogenic MHV3 serotype leads to a reversion of their intrinsic anti-inflammatory phenotype toward a proinflammatory profile as well to as disorders in vascular factors, correlating with the severity of hepatitis. These results highlight a new virus-promoted mechanism of exacerbation of liver inflammatory response during acute hepatitis.
ISSN: 0022-538X

hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/file/Mouse%20Hepatitis%20Virus%20Infection_accepted.pdf
DOI : 10.1128/JVI.01069-16

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : Plant litter diversity effects on decomposition rates are frequently reported, but with a strong bias towards temperate ecosystems. Altered decomposition and nutrient recycling with changing litter diversity may be particularly important in tree species-rich tropical rainforests on nutrient-poor soils. Using 28 different mixtures of leaf litter from 16 Amazonian rainforest tree species, we tested the hypothesis that litter mixture effects on decomposition increase with increasing functional litter diversity. Litter mixtures and all single litter species were exposed in the field for 9 months using custom-made microcosms with soil fauna access. In order to test the hypothesis that the long-term presence of tree species contributing to the litter mixtures increases mixture effects on decomposition, microcosms were installed in a plantation at sites including the respective tree species composition and in a nearby natural forest where these tree species are absent. We found that mixture decomposition deviated from predictions based on single species, with predominantly synergistic effects. Functional litter diversity, defined as either richness, evenness, or divergence based on a wide range of chemical traits, did not explain the observed litter mixture effects. However, synergistic effects in litter mixtures increased with the long-term presence of tree species contributing to these mixtures as the home field advantage hypothesis assumes. Our data suggest that complementarity effects on mixed litter decomposition may emerge through long-term interactions between aboveground and belowground biota.
ISSN: 0029-8549

hal-01032393
https://hal.archives-ouvertes.fr/hal-01032393
DOI : 10.1007/s00442-011-1966-4

Éditeur(s) : Public Library Science
Résumé : Changes in phenology, the timing of seasonal activities, are among the most frequently observed responses to environmental disturbances and in marine species are known to occur in response to climate changes that directly affects ocean temperature, biogeochemical composition and sea level. We examined nesting seasonality data from long-term studies at 8 green turtle (Chelonia mydas) rookeries that include 21 specific nesting sites in the South-West Indian Ocean (SWIO). We demonstrated that temperature drives patterns of nesting seasonality at the regional scale. We found a significant correlation between mean annual Sea Surface Temperature (SST) and dates of peak nesting with rookeries exposed to higher SST having a delayed nesting peak. This supports the hypothesis that temperature is the main factor determining peak nesting dates. We also demonstrated a spatial synchrony in nesting activity amongst multiple rookeries in the northern part of the SWIO (Aldabra, Glorieuses, Mohéli, Mayotte) but not with the eastern and southern rookeries (Europa, Tromelin), differences which could be attributed to females with sharply different adult foraging conditions. However, we did not detect a temporal trend in the nesting peak date over the study period or an inter-annual relation between nesting peak date and SST. The findings of our study provide a better understanding of the processes that drive marine species phenology. The findings will also help to predict their ability to cope with climate change and other environmental perturbations. Despite demonstrating this spatial shift in nesting phenology, no trend in the alteration of nesting dates over more than 20 years was found.
Plos One (1932-6203) (Public Library Science), 2012-10 , Vol. 7 , N. 10 , P. 1-13

Droits : 2012 Dalleau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://archimer.ifremer.fr/doc/00098/20940/18558.pdf
DOI:10.1371/journal.pone.0046920
http://archimer.ifremer.fr/doc/00098/20940/

Éditeur(s) : HAL CCSD American College of Sports Medicine (ACSM)
Résumé : International audience
PURPOSE AND METHODS: To determine the effects of cycling on a subsequent triathlon run, nine male triathletes underwent four successive laboratory trials: 1) an incremental treadmill test, 2) an incremental cycle test, 3) 30 min of cycling followed by 5 km of running (C-R), and 4) 30 min of running followed by 5 km of running (R-R). Before and 10 min after the third and fourth trials, the triathletes underwent pulmonary function testing including spirometry and diffusing capacity testing for carbon monoxide (DL(CO)). During the C-R and R-R trials, arterialized blood samples were obtained to measure arterial oxygen pressure (PaO2). During all trials, ventilatory data were collected every minute using an automated breath-by-breath system. RESULTS: The results showed that 1) the oxygen uptake (VO2) observed during subsequent running was similar for the C-R and R-R trials; 2) the ventilatory response (VE) during the first 8 min of subsequent running was significantly greater in the C-R than in R-R trial (P < 0.05); 3) only the C-R trial induced a significant increase (P < 0.05) in residual volume (RV), functional residual capacity (FRC), and the ratio of residual volume to total lung capacity (RV/TLC); and 4) although a significant decrease (P < 0.05) in DL(CO) was noted after C-R, no difference between the two exercise trials was found for the maximal drop in PaO2. CONCLUSIONS: We concluded that 1) the C-R trial induced specific alterations in pulmonary function that may be associated with respiratory muscle fatigue and/or exercise-induced hypoxemia, and 2) the greater VE observed during the first minute of running after cycling was due to the specificity of cycling. This reinforces the necessity for triathletes to practice multi-trial training to stimulate the physiological responses experienced during the swim-cycle and the cycle-run transitions.
ISSN: 0195-9131

hal-00720412
https://hal.univ-antilles.fr/hal-00720412
https://hal.univ-antilles.fr/hal-00720412/document
https://hal.univ-antilles.fr/hal-00720412/file/Article.pdf

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Staphylococcus aureus is a highly versatile, opportunistic pathogen and the etiological agent of a wide range of infections in humans and warm-blooded animals. The epithelial surface is its principal site of colonization and infection. In this work, we investigated the cytopathic effect of S. aureus strains from human and animal origins and their ability to affect the host cell cycle in human HeLa and bovine MAC-T epithelial cell lines. S. aureus invasion slowed down cell proliferation and induced a cytopathic effect, resulting in the enlargement of host cells. A dramatic decrease in the number of mitotic cells was observed in the infected cultures. Flow cytometry analysis revealed an S. aureus-induced delay in the G2/M phase transition in synchronous HeLa cells. This delay required the presence of live S. aureus since the addition of the heat-killed bacteria did not alter the cell cycle. The results of Western blot experiments showed that the G2/M transition delay was associated with the accumulation of inactive cyclin-dependent kinase Cdk1, a key inducer of mitosis entry, and with the accumulation of unphosphorylated histone H3, which was correlated with a reduction of the mitotic cell number. Analysis of S. aureus proliferation in asynchronous, G1- and G2-phase-enriched HeLa cells showed that the G2 phase was preferential for bacterial infective efficiency, suggesting that the G2 phase delay may be used by S. aureus for propagation within the host. Taken together, our results divulge the potential of S. aureus in the subversion of key cellular processes such as cell cycle progression, and shed light on the biological significance of S. aureus-induced host cell cycle alteration.
ISSN: 1932-6203

hal-00873672
https://hal.archives-ouvertes.fr/hal-00873672
https://hal.archives-ouvertes.fr/hal-00873672/document
https://hal.archives-ouvertes.fr/hal-00873672/file/Staphylococcus_aureus-Induced.accepted.pdf
DOI : 10.1371/journal.pone.0063279

Éditeur(s) : HAL CCSD Royal Society, The
Résumé : International audience
Since its original description as a chordate, the Late Devonian Scaumenella mesacanthi has been interpreted alternately as a prochordate, a larval ostracoderm and an immature acanthodian. For the past 30 years, these minute specimens were generally considered as decayed acanthodians, most of them belonging to Triazeugacanthus affinis. Among the abundant material of `Scaumenella', we identified a size series of 188 specimens of Triazeugacanthus based on otolith characteristics. Despite taphonomic alteration, we describe proportional growth and progressive appearance of skeletal elements through size increase. Three ontogenetic stages are identified based on squamation extent, ossification completion and allometric growth. We demonstrate that what has been interpreted previously as various degrees of decomposition corresponds to ontogenetic changes.
ISSN: 1744-9561

hal-01545474
https://hal.archives-ouvertes.fr/hal-01545474
DOI : 10.1098/rsbl.2014.0950

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Rainbow is a dome-shaped massif at the 36°14′N nontransform offset along the Mid-Atlantic Ridge. It hosts three ultramafic-hosted hydrothermal sites: Rainbow is active and high temperature; Clamstone and Ghost City are fossil and low temperature. The MoMARDREAM cruises (2007, 2008) presented here provided extensive rock sampling throughout the massif that constrains the geological setting of hydrothermal activity. The lithology is heterogeneous with abundant serpentinites surrounding gabbros, troctolites, chromitites, plagiogranites, and basalts. We propose that a W dipping detachment fault, now inactive, uplifted the massif and exhumed these deep-seated rocks. Present-day deformation is accommodated by SSW-NNE faults and fissures, consistent with oblique teleseismic focal mechanisms and stress rotation across the discontinuity. Faults localize fluid flow and control the location of fossil and active hydrothermal fields that appear to be ephemeral and lacking in spatiotemporal progression. Markers of high-temperature hydrothermal activity (∼350°C) are restricted to some samples from the active field while a more diffuse, lower temperature hydrothermal activity (<220°C) is inferred at various locations through anomalously high As, Sb, and Pb contents, attributed to element incorporation in serpentines or microscale-sulfide precipitation. Petrographic and geochemical analyses show that the dominant basement alteration is pervasive peridotite serpentinization at ∼160–260°C, attributed to fluids chemically similar to those venting at Rainbow, and controlled by concomitant alteration of mafic-ultramafic units at depth. Rainbow provides a model for fluid circulation, possibly applicable to hydrothermalism at oceanic detachments elsewhere, where both low-temperature serpentinization and magmatic-driven high-temperature outflow develop contemporaneously, channeled by faults in the footwall and not along the detachment fault.
ISSN: 1525-2027

hal-01115281
https://hal.archives-ouvertes.fr/hal-01115281
https://hal.archives-ouvertes.fr/hal-01115281/document
https://hal.archives-ouvertes.fr/hal-01115281/file/Andreani_et_al-2014-Geochemistry%2C_Geophysics%2C_Geosystems.pdf
DOI : 10.1002/2014GC005269

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Maternal affective disorders are frequently treated with selective serotonin reuptake inhibitor medications (SSRIs); with up to 10% of women being prescribed these medications during pregnancy. Infant development depends on the early serotonergic environment, which is altered by perinatal SSRIs, raising concern about how these medications affect neural outcomes. While clinical and preclinical research suggests an impact of SSRIs on the developing brain, more research is needed to determine the effects on neuroplasticity, the serotonergic system, and the hypothalamic-pituitary-adrenal axis in neural regions mediating behavior. The current work investigated the effects of the SSRI, fluoxetine, on the serotonergic system in the prefrontal cortex (PFC) during pre-adolescence, and changes to synaptic markers and glucocorticoid receptor density in the cingulate cortex (medial PFC) of pre-adolescent and adult Sprague-Dawley male and female rats. To model aspects of Perinatal Depression and maternal anxiety, pre-gestational maternal stress was used resulting in male and female offspring from 4 groups: 1) control, 2) perinatal fluoxetine exposed, 3) pre-gestational maternal stress exposed, and 4) pre-gestational maternal stress + fluoxetine. Perinatal fluoxetine prevented the effects of maternal stress on 5-HT levels and 5-HT turnover ratio in the PFC of pre-adolescent offspring, particularly in females. However, pre-gestational stress reduced synaptophysin and PSD-95 densities in the cingulate cortex, effects that were more pronounced in males. Interestingly, perinatal fluoxetine exposure reduced GR density in adult males in this same brain area. Together, results show differential effects of perinatal SSRIs and pre-gestational maternal stress on neurodevelopment in the PFC of males and females.
ISSN: 0028-3908

hal-01647158
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647158
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647158/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647158/file/Gemmel%20-%20Perinatal%20fluoxetine%20prevents%20the%20effect.pdf
DOI : 10.1016/j.neuropharm.2017.10.009

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Pre-association of CD95 at the plasma membrane is mandatory for efficient death receptor signaling. This homotrimerization occurs through self-association of an extracellular domain called the pre-ligand assembly domain (PLAD). Using novel molecular and cellular tools, we confirmed that CD95-PLAD is necessary to promote CD95 multimerization and plays a pivotal role in the transmission of apoptotic signals. However, while a human CD95 mutant deleted of the previously described PLAD domain (amino acids 1 to 66) fails to interact with its wild-type counterpart and trigger autonomous cell death, deletion of amino acids 1 to 42 does not prevent homo- or hetero (human/mouse)-oligomerization of CD95, and thus does not alter transmission of the apoptotic signal. Overall, these findings indicate that the region between amino acids 43 to 66 corresponds to the minimal motif involved in CD95 homotypic interaction and is necessary to convey an efficient apoptotic signal. Interfering with this PLAD may represent a new therapeutic strategy for altering CD95-induced apoptotic and non-apoptotic signals.
ISSN: 1932-6203

hal-00873708
https://hal.archives-ouvertes.fr/hal-00873708
https://hal.archives-ouvertes.fr/hal-00873708/document
https://hal.archives-ouvertes.fr/hal-00873708/file/Precise_Mappingsur the_CD95-accepted.pdf
DOI : 10.1371/journal.pone.0046236