Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
CYLD is a tumour suppressor gene mutated in familial cylindromatosis, a genetic disorder leading to the development of skin appendage tumours. It encodes a deubiquitinating enzyme that removes Lys63- or linear-linked ubiquitin chains. CYLD was shown to regulate cell proliferation, cell survival and inflammatory responses, through various signalling pathways. Here we show that CYLD localizes at centrosomes and basal bodies via interaction with the centrosomal protein CAP350 and demonstrate that CYLD must be both at the centrosome and catalytically active to promote ciliogenesis independently of NF-κB. In transgenic mice engineered to mimic the smallest truncation found in cylindromatosis patients, CYLD interaction with CAP350 is lost disrupting CYLD centrosome localization, which results in cilia formation defects due to impairment of basal body migration and docking. These results point to an undiscovered regulation of ciliogenesis by Lys63 ubiquitination and provide new perspectives regarding CYLD function that should be considered in the context of cylindromatosis.
ISSN: 2041-1723

hal-01063915
https://hal.archives-ouvertes.fr/hal-01063915
DOI : 10.1038/ncomms5585
PUBMED : 25134987

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The platinum-group element (PGE) systematics of continental mantle peridotites show large variability, reflecting petrogenetic processing of the upper mantle during partial melting and melt/fluid percolation inside the lithosphere. By removing Pd-Cu-Ni rich sulfides, partial melting events that have stabilized the sub-continental mantle lithosphere fractionated PPGEs (Palladium-group PGE; Pt, Pd) relative to IPGEs (Iridium-group PGE; Os, Ir, Ru, Rh). Residual base-metal sulfides (BMS) survive as enclosed IPGE-enriched Monosulfide Solid Solutions (Mss), which otherwise decompose into Ru-Os-Ir-rich refractory platinum-group minerals (PGMs) once the partial melts become S-undersaturated. The small-scale heterogeneous distribution of these microphases may cause extreme nugget effects, as seen in the huge variations in absolute PGE concentrations documented in cratonic peridotites. Magmas fluxing through the lithospheric mantle may change the initial PGE budgets inherited from the melting events, resulting in the great diversity of PGE systematics seen in peridotites from the sub-continental lithosphere. For instance, melt-rock reactions at increasing melt/rock ratios operate as open-system melting processes removing residual BMS/PGMs. Highly percolated peridotites are characterized by extreme PGE depletion, coupled with PGE patterns and Os-isotope compositions that gradually evolve toward that of the percolating melt. Reactions at decreasing melt-rock ratios (usually referred to as "mantle metasomatism") precipitate PPGE-enriched BMS that yield suprachondritic Pd/Ir and occasionally affect Pt/Ir and Rh/Ir ratios as well. Moreover, volatile-rich, small volume melts fractionate Os relative to Ir and S relative to Se, thereby producing rocks with supra-chondritic Os/Ir and S/Se coupled with supra-chondritic Pd/Ir and Pt/Ir. Major magmatic inputs at the lithosphere-asthenosphere boundary may rejuvenate the PGE systematics of the depleted mantle. Integrated studies of "refertilized" peridotites with worldwide provenance provide evidence for mixing between old PGM-rich harzburgitic protoliths and newly-precipitated BMS. Long-lived PGMs carry the Os-isotope compositions of ancient melt‐depletion events into seemingly undepleted fertile lherzolites. Another diagnostic feature of major refertilization processes is the increasing modal abundance of Pt-Pd-Te-Bi or Pt-As-S microphases. Due to regional-scale refertilization processes, sizeable (> 100 km) domains of the upper lithospheric mantle are now significantly enriched in Pd, Au, Cu, Se, and other incompatible chalcophile elements that are of considerable importance in PGE-ore forming events.
ISSN: 0024-4937

hal-00828626
https://hal.archives-ouvertes.fr/hal-00828626
DOI : 10.1016/j.lithos.2012.08.017

Éditeur(s) : HAL CCSD
Résumé : International audience
The Kazdağ Massif exposes a metamorphic dome in the Biga Peninsula of northwest Turkey. An extensional origin has been proposed for the dome, limited on both flanks by detachments and/or shear zones. The northern flank is bounded by the extensional Alakeçi Shear Zone (ASZ), whose P-T-t path is still poorly known. We therefore focus on its metamorphic conditions and related temporal history to precise its tectono-metamorphic evolution. The local tectonostratigraphy in structurally ascending order comprises: (i) the high-grade metamorphic core rocks of the Kazdaǧ Massif (gneisses and micaschists intercalated with amphibolites and marbles); (ii) the two kilometer-thick ASZ; (iii) the overlaying unmetamorphosed pre-Cenomanian accretionary Çetmi mélange; and (iv) Neogene sedimentary and volcanic cover rocks. ASZ mylonites were derived from both the core rocks and the mélange lithologies. From the north to the south the mylonitic fabrics in the ASZ depict a top-to-the N-NNE shearing, parallel to the NNE-plunging stretching lineation and NNW-dipping mylonitic foliation. This geometry implies normal sense movement i.e. north-side down-dip extensional displacement along this flank of the Kazdaǧ Massif. The northward transition from ductile to brittle-ductile regime through the ASZ shows that the deformation occurred at decreasing temperatures and degree of metamorphism. The paragenesis in equilibrium within the mylonitic gneisses and schists contains Qtz + Fs + Ms + Bt + Grt ± St ± Sill, with late retrogressive chlorite after biotite and garnet. Four samples of ASZ rocks yielded pressures between 6.9-5.7 kbar and temperatures between 706-587°C. Three samples from the mylonitic rocks supplied in situ isochron 36Ar/40Ar mica ages between 31.2-24.2 Ma, which we interpret to date the cooling of the mylonites following the P-T decrease across the ASZ. The metamorphic and structural results support the extensional character of the ASZ, and sketch transition from sillimanite core gneisses in the deeper structural levels to chlorite schists towards the top of the shear zone. These new data allow to precise the peak P-T conditions and the temporal evolution in the northern flank of the Kazdaǧ Massif, where Late Oligocene extensional exhumation was assisted by NNE-directed ductile-brittle ASZ, which had operated from amphibolite to greenschist facies. At the regional scale, this tectono-metamorphic pattern is similar to those observed on other places of the north Aegean domain.
IOP Conference Series
Austin, TE, United States

hal-00642725
https://hal-brgm.archives-ouvertes.fr/hal-00642725
DOI : 10.1088/1755-1307/2/1/012012

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The deep structure and deformation mechanisms of the Longmen Shan thrust belt (Sichuan, China), at the eastern border of the Tibetan plateau, were largely debated after the devastating Mw 7.9 Wenchuan earthquake (2008). Recent geophysical studies and field investigations have been focused on the active motion of the major Beichuan fault, which ruptured during the earthquake. However, the total exhumation across the fault still remains unclear. In the hanging wall of the Beichuan fault, the South China block is exhumed in the Pengguan massif. Close to the Beichuan fault, the rocks of the Pengguan massif underwent greenschist facies metamorphism associated with brittle-ductile deformation. No metamorphism is observed in the footwall of the fault. In this study, we characterize and date the metamorphic history recorded in the hanging wall of the Beichuan fault in order to constrain the depth and timing of exhumation of the rocks of the Pengguan massif along the fault. A high-resolution petrological approach involving chemical analyses and X-ray maps was used to analyze the micrometric metamorphic minerals. The P-T conditions of the greenschist facies metamorphic event were estimated by an inverse multi-equilibrium thermodynamic approach. The results, 280 ± 30 °C and 7 ± 1 kbar, suggest that the rocks of the Pengguan massif were exhumed from ca. 20 km depth. Our results underline the importance of the thrusting component in the long-term behavior of the Beichuan fault and provide a minimal depth at which the fault is rooted. In situ laser ablation 40Ar/39Ar dating of metamorphic white mica revealed that the greenschist overprint occurred at 135–140 Ma.The Pengguan massif was therefore partially thrusted along the Beichuan fault during the Lower Cretaceous, long before the Eocene-Miocene exhumation phase which is well-constrained by low-temperature thermochronology. Our results provide the first independent depth information for the exhumation history of the Pengguan massif and reveal a previously undocumented Lower Cretaceous tectonic event that marks the onset of the thick-skinned deformation in the external domain of the Longmen Shan (East of the Wenchuan Fault).
ISSN: 0743-9547

hal-01685556
https://hal.archives-ouvertes.fr/hal-01685556
DOI : 10.1016/j.jseaes.2017.04.003

Éditeur(s) : HAL CCSD Wiley
Résumé : The evolutionary potential of long-lived species, such as forest trees, is fundamental for their local persistence under climate change (CC). Genome–environment association (GEA) analyses reveal if species in heterogeneous environments at the regional scale are under differential selection resulting in populations with potential preadaptation to CC within this area. In 79 natural [i]Fagus sylvatica[/i] populations, neutral genetic patterns were characterized using 12 simple sequence repeat (SSR) markers, and genomic variation (144 single nucleotide polymorphisms (SNPs) out of 52 candidate genes) was related to 87 environmental predictors in the latent factor mixed model, logistic regressions and isolation by distance/environmental (IBD/IBE) tests. SSR diversity revealed relatedness at up to 150m intertree distance but an absence of large-scale spatial genetic structure and IBE. In the GEA analyses, 16 SNPs in 10 genes responded to one or several environmental predictors and IBE, corrected for IBD, was confirmed. The GEA often reflected the proposed gene functions, including indications for adaptation to water availability and temperature. Genomic divergence and the lack of large-scale neutral genetic patterns suggest that gene flow allows the spread of advantageous alleles in adaptive genes. Thereby, adaptation processes are likely to take place in species occurring in heterogeneous environments, which might reduce their regional extinction risk under CC
ISSN: 0028-646X

hal-01309135
https://hal.archives-ouvertes.fr/hal-01309135
PRODINRA : 343710
DOI : 10.1111/nph.13809

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Oisans Massif, located in the external zones of the western Alps, experienced significant shortening during the Alpine collision. While a series of major top-to-the west shear zones was recently described, the general low grade of the metamorphism has not attracted much petrological and geochronological studies. This paper provides combined temperature and age constraints on the evolution of the Oisans Massif. Temperature was estimated with the Raman Spectrometry of Carbonaceous Material (RSCM) method and chlorite geothermometry. Maximum temperature reached by the Mesozoic cover (Tmax) from Grenoble to the Galibier pass (E-W) and from Saint Jean de Maurienne to Embrun (N-S) yielded almost constant ca. 330 °C temperatures all through the Massif. Temperatures however strongly decrease either westward towards the top of the Vercors sedimentary sequence or eastward towards the Penninic Frontal Thrust. Age constraints were retrieved using 40Ar/39Ar in situ analyses performed on variously strained samples from Alpine shear zones. Over strain gradients, incipient Alpine recrystallizations progressively develop at the expense of former Variscan parageneses. A combined textural and EPMA approach permitted to identify newly formed chlorite and phengite that unequivocally grew in response to deformation or to low grade metamorphism. Chlorites recorded temperatures from ca. 350–150 °C during the activity of shear zones. In parallel, 40Ar/39Ar in situ experiments enabled dating deformation using both synkinematic phengites crystallized below the closure temperature of white-micas and former Variscan muscovite whose isotopic system have been, at least partially re-opened. Activity of top-to-the-west shear zones responsible for the shortening and thickening of the Oisans massif thus occurred between 34 and 33 and 25 Ma. Integrating these new age-constraints, Tmax estimates, and published geological data on the Oisans Massif and neighbouring areas allow proposing a new shortening scenario for the external zones. From ca. 34–33 Ma, the Oisans Massif was buried as a rigid block below more internal units and reached a temperature of ca. 330 °C. Shearing progressively localized along a series of top-to-the-W shear zones in the basement until 25 Ma. Deformation then localized along a major thrust at the base of the ECM massif and propagated along the basement-cover interface below the Vercors massif after 16 Ma.
ISSN: 0264-3707

Droits : info:eu-repo/semantics/OpenAccess
insu-01093878
https://hal-insu.archives-ouvertes.fr/insu-01093878
https://hal-insu.archives-ouvertes.fr/insu-01093878/document
https://hal-insu.archives-ouvertes.fr/insu-01093878/file/GEOD1326RA.pdf
DOI : 10.1016/j.jog.2014.09.004

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Extractives that provide natural resistance to Bagassa guianensis Aubl. heartwood were examined. Soil-bed tests showed that the B. guianensis heartwood resistance was significantly reduced after ethyl acetate extraction, whereas methanol and especially water extractions improved the resistance. The ethyl acetate extract was submitted to a bioguided fractionation, and fractions were tested against one wood-destroying fungal strain (Pycnoporus sanguineus) and two human pathogenic fungal strains (Candida glabrata (yeast) and Trichophyton rubrum (filamentous dermatophyte)). Fraction F7, which exhibited the strongest antifungal activity, was subsequently fractionated by high performance liquid chromatography (HPLC). Six previously described compounds were isolated. Although the two moracins, 6-O-methyl-moracin N (3) and moracin N (4) were the most active against fungal strains with MIC values between 4 and 16 μg ml−1, the isolated compounds showed less or equivalent antifungal activity than the initial fraction. Possible synergism between compounds 3 and 4 and other secondary metabolites have been hypothesized. Our study demonstrated that this extract as a whole might be used as a wood preservation or antimycotic product.
International Biodeterioration and Biodegradation

hal-00694532
https://hal.archives-ouvertes.fr/hal-00694532
DOI : 10.1016/j.ibiod.2011.10.016

Résumé : En raison de sa richesse en ressources génétiques, et des utilisations traditionnelles locales qui en sont faites, la biodiversité végétale issue du bassin amazonien constitue une véritable source de principes actifs à valoriser. L’espèce Euterpe oleracea Mart., vernaculairement appelée baie d’açai, qui connaît un intérêt scientifique important, est le parfait exemple de ressources naturelles bioactives valorisées issues de cette zone géographique. Les études scientifiques lui confèrent de très nombreuses propriétés biologiques, mais, la plus connue et la plus médiatisée est sa capacité antioxydante liée majoritairement à sa composition polyphénolique. En Guyane française, on peut parler de « diversité » au sein de la famille des Palmiers puisque plus de 75 espèces y ont été recensées. Parmi lesquelles, on peut citer une espèce très commune ayant des propriétés surtout alimentaires, et dont la connaissance phytochimique reste, à l’heure actuelle, très limitée : l’Oenocarpus bataua Mart dit patawa. Ce sujet de thèse de doctorat s’articule autour de la mesure de l’activité antioxydante du fruit mûr de ce palmier, et de la détermination des polyphénols responsables de cette dernière. La singularité de ce travail réside dans l’étude des différentes composantes tissulaires du fruit : mésocarpe, épicarpe et mélange épicarpe/mésocarpe (MEM). Dans un premier temps, les conditions les plus favorables d’extraction de biomolécules (notamment de l’épicarpe et du mésocarpe) ont été définies à l’aide du test DPPH. Les tests préliminaires effectués sur les tissus pris séparément, ont conduit à sélectionner un mélange acétone/eau (70/30, v/v) pour révéler, au mieux, la capacité antioxydante de chaque partie du fruit. Une étape de délipidation initiale s’est avérée nécessaire dans le cas de l’étude du mésocarpe. La confirmation de l’activité antioxydante a été réalisée au moyen d’autres tests d’activité chimique (TEAC, FRAP, ORAC), et a été complétée par l’utilisation d’un test d’activité biologique (KRL) en raison de ses mécanismes réactionnels plus complets. Il en ressort que le tissu végétal le plus antioxydant est le mésocarpe qui contient des proanthocyanidines, famille de composés phénoliques connue pour ses nombreuses activités biologiques.Le même travail a été effectué sur les tissus regroupés (fruit global ou MEM). Ainsi, a été retenue l’utilisation du solvant mixte acétone/eau sans étape de délipidation initiale. La capacité antioxydante du fruit étudié a été comparée à celle de l’açai, espèce choisie comme référence. Il s’avère que les extraits d’açai ont une activité antioxydante très supérieure à celle du patawa lorsqu’ils sont testés vis-à-vis de l’ORAC et du KRL. Le tissu mésocarpe a, lui, démontré une capacité antioxydante supérieure à celle de l’açai. Ces résultats sont à associer avec la composition phytochimique propre à chaque fruit. La composition polyphénolique du fruit de patawa, déterminée par UPLC/MSn, supposerait la présence d’anthocyanes, de tanins condensés, de stilbènes et d’acides phénoliques. Ces travaux, qui méritent d’être approfondis, en particulier pour le mésocarpe, ouvrent de nouvelles perspectives d’utilisation du fruit patawa, en particulier l’incorporation de composés phénoliques issus du mésocarpe dans des formulations galéniques ayant attrait aux domaines de la Nutrition, de la Cosmétique et de la Pharmaceutique.
Due to its wealth in genetic resources, and to traditional uses, plant biodiversity issued from the Amazonian Basin is a real source of active process to valorize. The specie Euterpeoleracea Mart., usually called acai berry, which is experiencing a huge scientific interest, is the perfect example of valued natural bioactive resources from the geographic area. Scientific studies give it many biological properties, but the most known is its antioxidant property mainly due to its polyphenolic composition. In French Guiana, we can use the term “diversity” within the palm family with more than 75 species identified. Among them is a common species, Oenocarpus bataua Mart., called “Patawa”, mainly with alimentary properties but for which knowledge of phytochemical properties is until now very poor. The present research deals with determining the antioxidant activity of this palm fruit and with the identification of the polyphenols responsible for it.The uniqueness of this work lays in the study of the different tissue components of this fruit namely the mesocarp, the epicarp and mixing epicarp / mesocarp (MEM). In a first time, the most favorable extraction conditions of biomolecules (particularly of the epicarp and mesocarp) were defined using the DPPH test. The preliminary tests performed on those tissues taken separately, have led to select an acetone / water (70/30, v / v) to reveal, at best, the antioxidant capacity of each part of the fruit. An initial defatting step was necessary in the case of the study of the mesocarp. The confirmation of the antioxidant activity was carried out by other tests of chemical activity (TEAC, FRAP, ORAC), and was supplemented by the use of a bioassay (KRL) due to its more complete reaction mechanisms. Results point out that the most antioxidant tissue is the mesocarp that contains proanthocyanidins, phenolics of a chemical family known for its numerous biological activities.The same work was performed on tissues combined (overall result). The mixed solvent acetone / water, without initial defatting step, has been selected. The antioxidant capacity of fruit was compared to that of the Acai specie chosen as a reference. It turns out that acai extracts have antioxidant activity much greater than that of Patawa when tested vis-à-vis of ORAC and KRL. In contrary, mesocarp tissue has a greater antioxidant capacity than that of Acai. These results can be associated with the phytochemical composition of each fruit. The polyphenolic composition of the fruit of Patawa determined by UPLC / MSn, reflects the presence of anthocyanins, condensed tannins, stilbene and phenolic acids. This work, which deserves to be deepened, especially for the mesocarp tissue, opens new prospects for the use of Patawa fruit, especially the incorporation of phenolic compounds from the mesocarp in pharmaceutical formulations linked to the fields of Nutrition, of Cosmetics and Pharmaceuticals.

http://www.theses.fr/2012AGUY0573/document

Éditeur(s) : Amer Geophysical Union
Résumé : The hydrological and geochemical properties of the waters constituting the Pacific Equatorial Undercurrent (EUC) determine the properties of the equatorial cold tongue. Understanding and quantifying the various EUC origins is therefore of prime importance. For this purpose, a high-resolution (1/4 degrees) interannual oceanic simulation was analyzed from the western tropical Pacific boundaries to 140 degrees W, using a Lagrangian framework. Waters from the Low-Latitude Western Boundary Currents (LLWBCs) transiting from Vitiaz Strait (the main contributor), from Solomon Strait, and via the Mindanao Current were identified as the principal sources to the EUC. Waters conveyed by the interior ocean off equator are negligible till 180 E. The LLWBCs' waters represent 87% of the EUC transport at 156 degrees E out of which the New Guinea Coastal Undercurrent (NGCU) is as large as 47%. The EUC meridional distribution suggests that the waters originating from Solomon Strait and Mindanao Current mostly remain in the hemisphere from which they originate. Contrastingly, Vitiaz Strait waters are found in both hemispheres. The vertical EUC distribution shows that the lower layer of the EUC is mainly composed of Vitiaz Strait waters. Finally, the source transport distributions were characterized, at their origin and within the EUC, as a function of density. These distributions showed that waters flowing through Vitiaz Strait at densities higher than those of the EUC (down to sigma(theta) = 27.2 kg m(-3)) undergo a diapycnal mixing and lighten during their journey to join the EUC. This lightening supports the suggestion that the NGCU is a major source for the EUC geochemical enrichment.
Journal Of Geophysical Research-oceans (0148-0227) (Amer Geophysical Union), 2011-12 , Vol. 116 , N. C12044 , P. 16 p.

Droits : 2011 by the American Geophysical Union
http://archimer.ifremer.fr/doc/00060/17105/14621.pdf
DOI:10.1029/2011JC007477
http://archimer.ifremer.fr/doc/00060/17105/

Éditeur(s) : HAL CCSD Wiley
Résumé : Tank bromeliads form a conspicuous, yet neglected freshwater habitat in Neotropical forests. Recent studies driven by interests in medical entomology, fundamental aspects of bromeliad ecology and experimental research on food webs have, however, prompted increasing interest in bromeliad aquatic ecosystems. As yet, there is nothing in the literature about the life histories and environmental drivers of invertebrate population dynamics in tank bromeliads. Based on fortnightly samples taken over oneyear, size frequency plots and individual dry masses allowed us to establish the life cycles and growth rates of the dominant aquatic invertebrates in a common bromeliad species of French Guiana. Linear mixed-effect models and Mantel tests were used to predict changes in density, biomass, and growth rates in relation to temperature, rainfall, humidity and detrital resources. Annual variations in invertebrate densities and biomasses could be described according to three types of distribution: unimodal, bimodal or almost constant. Despite seasonal variations, precipitation, temperature, relative humidity and detritus concentration accounted significantly for changes in density and biomass, but we found no significant responses in growth rates of most invertebrate species. Species rather displayed non-seasonal life cycles with overlapping cohorts throughout the year. There was also a trend for delayed abundance peaks among congeneric species sharing similar functional traits, suggesting temporal partitioning of available resources. Beyond novel knowledge, quantitative information on life histories is important to predict food-web dynamics under the influence of external forcing and self-organisation. Our results suggest that changes in species distribution that will affect population dynamics through biotic interactions in space and/or time could have greater effects on food webs and ecosystem functioning than changes in environmental factors per se.
ISSN: 0046-5070

hal-01607779
https://hal.archives-ouvertes.fr/hal-01607779
DOI : 10.1111/fwb.12862
PRODINRA : 407311

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
During the development of the Variscan orogeny, large amounts of granitic melt were produced, giving rise to the intrusion of granitoids at different structural levels. Despite numerous studies, ages available from previous work on the Cevennes granites remain largely imprecise. In order to better constrain the age and emplacement mode of these granites, we have combined U-Pb dating on monazites and zircons and Ar-40/Ar-39 dating on biotites with petrological observations, major element chemical analysis and SEM zircon imaging on five samples from the Aigoual-St Guiral-Liron and Mont Lozere granitic massifs. The results revealed that granitic intrusions and cooling in Southern Cevennes occurred in a short time span at similar to 306 Ma after the main episode of regional metamorphism. Petrological and chemical data suggest that they result from a mixing between mantle-derived basic magmas (lamprophyres) and lower crust acid magmas. At a regional scale the production of these melts occurred at the end of crustal thickening induced by nappe stacking, at the same time as the late anatectic events recorded further north in the Velay dome and the granulite facies metamorphism recorded in metasedimentary granulite enclaves brought up by Tertiary volcanoes of the Velay area (Bournac).
ISSN: 1437-3254

hal-00412183
https://hal.archives-ouvertes.fr/hal-00412183
DOI : 10.1007/s00531-007-0187-x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Highly siderophile elements (Platinum-group elements, Au and Re) are currently assumed to reside inside base metal sulfides (BMS) in the convecting upper mantle. However, fertile lherzolites sampled by Pyrenean orogenic peridotite massifs are unexpectedly rich in 0.5-3 mu m large micronuggets of platinum-group minerals (PGM). Among those, sulfides from the laurite-erlichmanite series (Ru, Os(Ir)S(As)2), Pt-Ir-Os alloys and Pt-Pd-Te-Bi phases (moncheite-merenskyite) are predominant. Not only the BMS phases but also the PGM micronuggets must be taken into account in calculation of the PGE budget of orogenic fertile lherzolites. Laurite is a good candidate for equilibrating the whole-rock budget of Os, it and Ru while accounting for supra-chondritic Ru/Ir-N. Textural relationships between PGMs and BMS highlight heterogeneous mixing between refractory PGMs (laurite/Pt-Ir-Os alloys) inherited from ancient refractory lithospheric mantle and late-magmatic metasomatic sulfides precipitated from tholeiitic melts. "Low-temperature" PGMs, especially Pt-Pd bismuthotellurides should be added to the list of mineral indicators of lithosphere refertilization process. Now disseminated within fertile lherzolites, "lithospheric" PGMs likely account for local preservation of ancient Os model ages (up to 2 Ga) detected in BMS by in-situ isotopic analyses. These PGMs also question the reliability of orogenic lherzolites for estimating the PGE signature of the Primitive Silicate Earth.
ISSN: 0012-821X

hal-00486109
https://hal.archives-ouvertes.fr/hal-00486109
DOI : 10.1016/j.epsl.2009.11.017

Éditeur(s) : HAL CCSD Springer
Résumé : International audience
In this study, we used data recorded by two consecutive passive broadband deployments on the Gulf of Aden northern margin, Dhofar region, Sultanate of Oman. The objective of these deployments is to map the young eastern Gulf of Aden passive continental margin crust and upper mantle structure and rheology. In this study, we use shear-wave splitting analysis to map lateral variations of upper mantle anisotropy beneath the study area. In this study, we found splitting magnitudes to vary between 0.33 and 1.0 s delay times, averaging about 0.6 s for a total of 17 stations from both deployment periods. Results show distinct abrupt lateral anisotropy variation along the study area. Three anisotropy zones are identified: a western zone dominated by NW-SE anisotropy orientations, an eastern zone dominat- ed with NE-SW anisotropy orientations, and central zone with mixed anisotropy orientations similar to the east and west zones. We interpret these shorter wavelength anisotropy zones to possibly represent fossil lithospheric mantle anisotropy. We postulate that the central anisotropy zone may be representing a Proterozoic suture zone that separates two terranes to the east and west of it. The anisotropy zones west and east were being used indicative of different terranes with different upper mantle anisotropy signatures.
ISSN: 1866-7411

hal-00647478
https://hal.archives-ouvertes.fr/hal-00647478
DOI : 10.1007/s12517-011-0477-2

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Androgen signaling, via the androgen receptor (AR), is crucial in mediating prostate cancer (PCa) initiation and progression. Identifying new downstream effectors of the androgens/AR pathway will allow a better understanding of these mechanisms and could reveal novel biomarkers and/or therapeutic agents to improve the rate of patient survival. We compared the microRNA expression profiles in androgen-sensitive LNCaP cells stimulated or not with 1 nM R1881 by performing a high-throughput reverse transcriptase-quantitative PCR and found that miR-135a was upregulated. After androgen stimulation, we showed that AR directly activates the transcription of miR-135a2 gene by binding to an androgen response element in the promoter region. Our findings identify miR-135a as a novel effector in androgens/AR signaling. Using xenograft experiments in chick embryos and adult male mice, we showed that miR-135a overexpression decreases in vivo invasion abilities of prostate PC-3 cells. Through in vitro wound-healing migration and invasion assays, we demonstrated that this effect is mediated through downregulating ROCK1 and ROCK2 expression, two genes that we characterized as miR-135a direct target genes. In human surgical samples from prostatectomy, we observed that miR-135a expression was lower in tumoral compared with paired adjacent normal tissues, mainly in tumors classified with a high Gleason score (⩾8). Moreover, miR-135a expression is lower in invasive tumors, showing extraprostatic extension, as compared with intraprostatic localized tumors. In tumor relative to normal glands, we also showed a more frequently higher ROCK1 protein expression determined using a semi-quantitative immunohistochemistry analysis. Therefore, in tumor cells, the lower miR-135a expression could lead to a higher ROCK1 protein expression, which could explain their invasion abilities. The highlighted relationship between miR-135a expression level and the degree of disease aggressiveness suggests that miR-135a may be considered as a prognostic marker in human PCa.Oncogene advance online publication, 28 July 2014; doi:10.1038/onc.2014.222.
ISSN: 0950-9232

hal-01063902
https://hal.archives-ouvertes.fr/hal-01063902
https://hal.archives-ouvertes.fr/hal-01063902/file/Androgen-regulated%20microRNA-135a.pdf
DOI : 10.1038/onc.2014.222
PUBMED : 25065599

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Anti-Müllerian hormone (AMH) plays crucial roles in sexual differentiation and gonadal functions. However, the possible extragonadal effects of AMH on the hypothalamic-pituitary-gonadal axis remain unexplored. Here we demonstrate that a significant subset of GnRH neurons both in mice and humans express the AMH receptor, and that AMH potently activates the GnRH neuron firing in mice. Combining in vivo and in vitro experiments, we show that AMH increases GnRH-dependent LH pulsatility and secretion, supporting a central action of AMH on GnRH neurons. Increased LH pulsatility is an important pathophysiological feature in many cases of polycystic ovary syndrome (PCOS), the most common cause of female infertility, in which circulating AMH levels are also often elevated. However, the origin of this dysregulation remains unknown. Our findings raise the intriguing hypothesis that AMH-dependent regulation of GnRH release could be involved in the pathophysiology of fertility and could hold therapeutic potential for treating PCOS
ISSN: 2041-1723

hal-01259206
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01259206
DOI : 10.1038/ncomms10055
PUBMED : 26753790

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We show that a new definition of the asymptotic structure of the (C,E,P)-algebras (which are a refinement of the Colombeau Generalized Algebras) allows to define a natural topology on them. Furthermore, this topology can be mixed with functorial aspects of the construction in order to give extension principles, which give powerful tools to solve singular differential problems in this framework.
ISSN: 0022-247X

hal-01529551
https://hal.univ-antilles.fr/hal-01529551
DOI : 10.1016/j.jmaa.2009.05.046

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Most peridotite massifs and mantle xenoliths show a wide range of isotopic variations, often involving significant decoupling between Hf, Nd and Sr isotopes. These variations are generally ascribed either to mingling of individual components of contrasted isotopic compositions or to time integration of parent-element enrichment by percolating melts/fluids, superimposed onto previous depletion event(s). However, strong isotopic decoupling may also arise during porous flow as a result of daughter-elements fractionation during solid-liquid interaction. Although porous flow is recognized as an important process in mantle rocks, its effects on mantle isotopic variability have been barely investigated so far. The peridotites of the Lherz massif (French Pyrenees) display a frozen melt percolation front separating highly refractory harzburgites from refertilized lherzolites. Isotopic signatures observed at the melt percolation front show a strong decoupling of Hf from Nd and Sr isotopes that cannot be accounted for by simple mixing involving the harzburgite protolith and the percolating melt. Using one dimensional percolation-diffusion and percolation-reaction modeling, we show that these signatures represent transient isotopic compositions generated by porous flow. These signatures are governed by a few critical parameters such as daughter element concentrations in melt and peridotite, element diffusivity, and efficiency of isotopic homogenization rather than by the chromatographic effect of melt transport and the refertilization reaction. Subtle variations in these parameters may generate significant inter-isotopic decoupling and wide isotopic variations in mantle rocks. (C) 2009 Elsevier B.V. All rights reserved.
ISSN: 0012-821X

hal-00413135
https://hal.archives-ouvertes.fr/hal-00413135
DOI : 10.1016/j.epsl.2008.12.033

Éditeur(s) : Oxford Univ Press Inc
Résumé : Since the transition from terrestrial to marine environments poses strong osmoregulatory and energetic challenges, temporal and spatial fluctuations in oceanic salinity might influence salt and water balance (and hence, body condition) in marine tetrapods. We assessed the effects of salinity on three species of sea snakes studied by mark-recapture in coral-reef habitats in the Neo-Caledonian Lagoon. These three species include one fully aquatic hydrophiine (Emydocephalus annulatus), one primarily aquatic laticaudine (Laticauda laticaudata), and one frequently terrestrial laticaudine (Laticauda saintgironsi). We explored how oceanic salinity affected the snakes' body condition across various temporal and spatial scales relevant to each species' ecology, using linear mixed models and multimodel inference. Mean annual salinity exerted a consistent and negative effect on the body condition of all three snake species. The most terrestrial taxon (L. saintgironsi) was sensitive to salinity over a short temporal scale, corresponding to the duration of a typical marine foraging trip for this species. In contrast, links between oceanic salinity and body condition in the fully aquatic E. annulatus and the highly aquatic L. laticaudata were strongest at a long-term (annual) scale. The sophisticated salt-excreting systems of sea snakes allow them to exploit marine environments, but do not completely overcome the osmoregulatory challenges posed by oceanic conditions. Future studies could usefully explore such effects in other secondarily marine taxa such as seabirds, turtles, and marine mammals.
Integrative And Comparative Biology (1540-7063) (Oxford Univ Press Inc), 2012-08 , Vol. 52 , N. 2 , P. 235-244

Droits : The Author 2012. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. All rights reserved.
http://archimer.ifremer.fr/doc/00089/20064/20739.pdf
DOI:10.1093/icb/ics081
http://archimer.ifremer.fr/doc/00089/20064/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Massif du Sud is a large ophiolitic complex that crops out in the southern region of New Caledonia (SW Pacific). It is dominated by harzburgite tectonite that locally shows a transitional gradation to massive dunite up section. Clinopyroxene, orthopyroxene and plagioclase progressively appear in dunite up to the transition to layered wehrlite and orthopyroxene-gabbro. The dunite-wehrlite and wehrlite-gabbro contacts are parallel and the latter defines the paleo-Moho.;Highly depleted modal, mineral and bulk rock compositions indicate that harzburgites are residues after high degrees (20-30%) of partial melting mainly in the spinet-stability field. Their relative enrichment in HFSE, LREE and MREE is due to re-equilibration of melting residues with percolating melts. Dunite formed in the Moho transition zone by reaction between residual mantle harzburgite and olivine-saturated melts that led to pyroxene dissolution and olivine precipitation. Rare clinopyroxene and plagioclase crystallized in interstitial melt pores of dunite from primitive, low-TiO2, ultra-depleted liquids with a geochemical signature transitional between those of island arc tholeiites and boninites.;Ascending batches of relatively high-SiO2, ultra-depleted melts migrated through the Moho transition zone and generated wehrlite by olivine dissolution and crystallization of clinopyroxene, orthopyroxene and plagioclase in variable amounts. These liquids were more evolved and were produced by higher degrees of melting or from a more depleted source compared with melts that locally crystallized clinopyroxene in dunite. Ultra-depleted magmas, non-cogenetic with those that formed the Moho transition zone, ascended to the lower crust and generated gabbroic cumulates with subduction-related affinity. Thus, the ultramafic and mafic rocks in the Moho transition zone and lower crust of the Massif du Sud ophiolite are not products of fractional crystallization from a single magma-type but are the result of migration and accumulation of different melts in a multi-stage evolution. The record of high partial melting in the mantle section, and migration and accumulation of ultra-depleted subduction-related melts in the Moho transition zone and lower crust support that the Massif du Sud ophiolite is a portion of forearc lithosphere generated in an extensional regime during the early phases of the subduction zone evolution. Our results show the existence of different types of ultra-depleted melt compositions arriving at the Moho transition zone and lower crust of an infant intraoceanic paleo-arc. Ultra-depleted melts may thus be a significant component of the melt budget generated in oceanic spreading forearcs prior to aggregation and mixing of a large range of melt compositions in the crust.
ISSN: 0009-2541

hal-00429333
https://hal.archives-ouvertes.fr/hal-00429333
DOI : 10.1016/j.chemgeo.2009.06.004

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The timing of E–W extension of the Tibetan plateau provides a test of mechanical models of the geodynamic evolution of the India–Asia convergence zone. In this work we focus on the Kung Co half graben (Southern Tibet, China), bounded by an active N–S normal fault with a minimum vertical offset of 1600 m. To estimate the onset of normal faulting we combined high and medium temperature (U–Pb, Ar/Ar) and low temperature ((U–Th)/He) thermochronometry of the Kung Co pluton, a two-mica granite of the northern Himalayan granitic belt that outcrop in the footwall of the fault. Biotite and muscovite Ar/Ar ages , are close from each other [not, vert, similar 16 Ma ± 0.2 (Ms) and not, vert, similar 15 ± 0.4 Ma (Bt)], which is typical of fast cooling. The zircon and apatite (U–Th)/He ages range from 11.3 to 9.6 Ma and 9.9 to 3.7 Ma respectively. These He ages are indicative of (1) fast initial cooling, from 11.3 to not, vert, similar 9 Ma, gradually decreasing with time and (2) a high geothermal gradient (not, vert, similar 400 °C/km), close to the surface at not, vert, similar 10 Ma. The Kung Co pluton was emplaced at about 22 Ma (U–Pb on zircon) at less than 10 km depth and 520–545 °C. Subsequent to its shallow emplacement, the pluton underwent fast thermal re-equilibration ending around 7.5 Ma, followed by a period of slow cooling caused either by the end of the thermal re-equilibration or by very slow exhumation (0.02–0.03 mm/yr) from not, vert, similar 7.5 Ma to at least 4 Ma. In either case the data suggest that the exhumation rate increased after 4 Ma. We infer this increase to be related to the initiation of the Kung Co normal fault. A critical examination of previously published data show that most not, vert, similar N–S Tibetan normal faults may have formed less than 5 Ma ago rather than in the Miocene as assumed by several authors. Such a young age implies that E–W extension is not related to the Neogene South Tibetan magmatism (25 to 8 Ma). Consequently, models relating E–W extension to magmatism, such as convective removal of the lower lithosphere, may be inappropriate. We rather think that this extension is related with local accommodation of boundary forces and displacements.
ISSN: 0012-821X

hal-00311791
https://hal.archives-ouvertes.fr/hal-00311791
DOI : 10.1016/j.epsl.2007.01.029