Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : In this work we combined apatite fission track and biotite/K-feldspar 40Ar/39Ar ages with tectonic data in the west central part of the Axial Zone of the Pyrenees. We discuss the exhumation ages and rates of the Néouvielle, Bordère-Louron, and Bielsa Variscan granites and their relationships with the timing and sequence of south vergent basement thrusting within the Pyrenean orogenic prism. The 40Ar/39Ar ages on K-feldspars from the Néouvielle massif (sample NV7) seem to indicate tectonic movements on the Eaux-Chaudes thrust during the early middle Eocene. Fission track results suggest that the exhumation of the Néouvielle massif occurred around 35 Ma and exhumation of the Bordère-Louron massif around 32 Ma in relation to thrusting on the Gavarnie thrust. The Bielsa massif was exhumed from around 19 Ma by out-of-sequence movements on the Bielsa thrust. We thus show that whereas most of the Pyrenean basement thrust faults (here the Eaux-Chaudes, Gavarnie, and Guarga thrusts) were active in sequence toward the southern foreland from the early Eocene to the earliest Miocene, some of them (here the Bielsa thrust) were activated out of sequence in the hinterland, later than the generally accepted Aquitanian age for the end of the Pyrenean compression. Finally, the apatite fission track modeling indicate a last cooling episode starting around 5 Ma which is most certainly related to the Pliocene reexcavation of the southern and northern flanks of the Pyrenees
ISSN: 0278-7407

hal-00406582
https://hal.archives-ouvertes.fr/hal-00406582
DOI : 10.1029/2006TC002080

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
IODP Site U1309 was drilled at Atlantis Massif, an oceanic core complex, at 30 degrees N on the Mid-Atlantic Ridge (MAR). We present the results of a bulk rock geochemical study (major and trace elements) carried out on 228 samples representative of the different lithologies sampled at this location. Over 96% of Hole U1309D is made up of gabbroic rocks. Diabases and basalts cross-cut the upper part of the section; they have depleted MORB compositions similar to basalts sampled at MAR 30 degrees N. Relics of mantle were recovered at shallow depth. Mantle peridotites show petrographic and geochemical evidence of extensive melt-rock interactions. Gabbroic rocks comprise: olivine-rich troctolites (>70% modal olivine) and troctolites having high Mg# (82-89), high Ni (up to 2300 ppm) and depleted trace element compositions (Yb 0.06-0.8 ppm); olivine gabbros and gabbros (including gabbronorites) with Mg# of 60-86 and low trace element contents (Yb 0.125-2.5 ppm); and oxide gabbros and leucocratic dykes with low Mg# (<50), low Ni (similar to 65 ppm) and high trace element contents (Yb up to 26 ppm). Troctolites and gabbros are amongst the most primitive and depleted oceanic gabbroic rocks. The main geochemical characteristics of Site U1309 gabbroic rocks are consistent with a formation as a cumulate sequence after a common parental MORB melt, although (lack of systematic) downhole variations indicate that the gabbroic series were built by multiple magma injections. In detail, textural and geochemical variations in olivine-rich troctolites and gabbronorites suggest chemical interaction (assimilation?) between the parental melt and the intruded lithosphere. Site U1309 gabbroic rocks do not represent the complementary magmatic product of 30 degrees N volcanics, although they sample the same mantle source. The bulk trace element composition of Site U1309 gabbroic rocks is similar to primitive MORB melt compositions; this implies that there was no large scale removal of melts from this gabbro section. The occurrence of such a large magmatic sequence implies that a high magmatic activity is associated with the formation of Atlantis Massif. Our results suggest that almost all melts feeding this magmatic system stays trapped into the intruded lithosphere.
ISSN: 0012-821X

hal-00413562
https://hal.archives-ouvertes.fr/hal-00413562
DOI : 10.1016/j.epsl.2008.12.034

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0168-583X

hal-01685803
https://hal.univ-antilles.fr/hal-01685803
DOI : 10.1016/0168-583X(93)90750-Z

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0257-8972

hal-01685814
https://hal.univ-antilles.fr/hal-01685814
DOI : 10.1016/0257-8972(92)90273-D

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0168-583X

hal-01685818
https://hal.univ-antilles.fr/hal-01685818
DOI : 10.1016/0168-583X(91)95793-D

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Expeditions 304 and 305 of the Integrated Ocean Drilling Program cored and logged a 1.4 km section of the domal core of Atlantis Massif. Postdrilling research results summarized here constrain the structure and lithology of the Central Dome of this oceanic core complex. The dominantly gabbroic sequence recovered contrasts with predrilling predictions; application of the ground truth in subsequent geophysical processing has produced self-consistent models for the Central Dome. The presence of many thin interfingered petrologic units indicates that the intrusions forming the domal core were emplaced over a minimum of 100-220 kyr, and not as a single magma pulse. Isotopic and mineralogical alteration is intense in the upper 100 m but decreases in intensity with depth. Below 800 m, alteration is restricted to narrow zones surrounding faults, veins, igneous contacts, and to an interval of locally intense serpentinization in olivine-rich troctolite. Hydration of the lithosphere occurred over the complete range of temperature conditions from granulite to zeolite facies, but was predominantly in the amphibolite and greenschist range. Deformation of the sequence was remarkably localized, despite paleomagnetic indications that the dome has undergone at least 45 degrees rotation, presumably during unroofing via detachment faulting. Both the deformation pattern and the lithology contrast with what is known from seafloor studies on the adjacent Southern Ridge of the massif. There, the detachment capping the domal core deformed a 100 m thick zone and serpentinized peridotite comprises similar to 70% of recovered samples. We develop a working model of the evolution of Atlantis Massif over the past 2 Myr, outlining several stages that could explain the observed similarities and differences between the Central Dome and the Southern Ridge.
ISSN: 0148-0227

hal-00617528
https://hal.archives-ouvertes.fr/hal-00617528
https://hal.archives-ouvertes.fr/hal-00617528/document
https://hal.archives-ouvertes.fr/hal-00617528/file/2010JB007931.pdf
DOI : 10.1029/2010JB007931

Éditeur(s) : HAL CCSD
Résumé : International audience
BACKGROUND: Africa faces a number of unique environmental challenges. Unfortunately, it lacks the infrastructure needed to support the comprehensive environmental studies that could provide the scientific basis to inform environmental policies. There are a number of known sources of endocrine-disrupting chemicals (EDCs) and other hazardous chemicals in Africa. However, a coordinated approach to identify and monitor these contaminants and to develop strategies for public health interventions has not yet been made. OBJECTIVES: This commentary summarizes the scientific evidence presented by experts at the First African Endocrine Disruptors meeting. We describe a "call to action" to utilize the available scientific knowledge to address the impact of EDCs on human and wildlife health in Africa. DISCUSSION: We identify existing knowledge gaps about exposures to EDCs in Africa and describe how well-designed research strategies are needed to address these gaps. A lack of resources for research and a lag in policy implementation slows down intervention strategies and poses a challenge to advancing future health in Africa. CONCLUSION: To address the many challenges posed by EDCs, we argue that Africans should take the lead in prioritization and evaluation of environmental hazards, including EDCs. We recommend the institution of education and training programs for chemical users, adoption of the precautionary principle, establishment of biomonitoring programs, and funding of community-based epidemiology and wildlife research programs led and funded by African institutes and private companies. https://doi.org/10.1289/EHP1774.
Environmental Health Perspectives

hal-01616487
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616487
DOI : 10.1289/EHP1774
PUBMED : 28935616

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0168-583X

hal-01685822
https://hal.univ-antilles.fr/hal-01685822
DOI : 10.1016/0168-583X(90)90675-K

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Facts -The capacity to exercise is markedly reduced in hot environments. -High humidity imposes further physiological strain on the body. -The combination of exercise and hot environments increases the risk of heat illness and heatstroke. Recommendations for players -Fluid intake should be sufficient to restrict body mass loss to o2% of the pre-exercise mass. -A minimum of 1-2 weeks of acclimatization should be undertaken before playing in a hot environment. Recommendations for organizers -Perform pre-participation screenings before competing in the heat. -Watch at-risk individual (e.g. sickle cell trait, vascular disease) and observe signs and symptoms if WBGT > 28°C. -Add additional fluid breaks and foresee medical coverage if WBGT > 30°C. -Reconsider event if WBGT > 32°C. Future research should investigate -The optimal cooling strategies prior and during sporting activities, i.e., halftime in football games. -The influence of acclimatization on football performance. -The impact of heat on decision making in players and match officials. -The impact of heat on spectators from cooler regions.
ISSN: 0905-7188

hal-00706256
https://hal.univ-antilles.fr/hal-00706256