Éditeur(s) : HAL CCSD Elsevier
Résumé : Eperua falcata (Aublet), a late-successional species in tropical rainforest and one of the most abundant tree in French Guiana, has developed an original strategy concerning N-acquisition by largely preferring nitrate, rather than ammonium (H. Schimann, S. Ponton, S. Hattenschwiler, B. Ferry, R. Lensi, A.M. Domenach, J.C. Roggy, Differing nitrogen use strategies of two tropical rainforest tree species in French Guiana: evidence from N-15 natural abundance and microbial activities, Soil Biol, Biochem. 40 (2008) 487-494). Given the preference of this species for nitrate, we hypothesized that root exudates would promote nitrate availability by (a) enhancing nitrate production by stimulating ammonium oxidation or (b) minimizing nitrate losses by inhibiting denitrification. Root exudates were collected in situ in monospecific planted plots. The phytochemical analysis of these exudates and of several of their corresponding root extracts was achieved using UHPLC/DAD/ESI-QTOF and allowed the identification of diverse secondary metabolites belonging to the flavonoid family. Our results show that (i) the distinct exudation patterns observed are related to distinct root morphologies, and this was associated with a shift in the root flavonoid content, (ii) a root extract representative of the diverse compounds detected in roots showed a significant and selective metabolic inhibition of isolated denitrifiers in vitro, and (iii) in soil plots the abundance of nirK-type denitrifiers was negatively affected in rhizosphere soil compared to bulk. Altogether this led us to formulate hypothesis concerning the ecological role of the identified compounds in relation to N-acquisition strategy of this species. (C) 2013 Elsevier Masson SAS. All rights reserved.
ISSN: 0981-9428

hal-01032437
https://hal.archives-ouvertes.fr/hal-01032437
DOI : 10.1016/j.plaphy.2013.05.003

Éditeur(s) : HAL CCSD Elsevier
Résumé : Understanding the pattern in spatial distribution of soil microbial processes is critical to understand the environmental factors that regulate them as well as to scale up these processes to ecosystem. Soil samples from a I ha tropical rainforest plot (Paracou, French Guiana) were analyzed according a nested sampling approach using different separation distances ranging from 0.4 to 40 m. The variability of substrate induced respiration (SIR) and of denitrification enzyme activity (DEA) was characterized in relation to various soil properties (total C and N contents, NIRS related index of soil organic matter quality, SOMQ and index of tree influence potential, IP). The variability of SIR and DEA was higher than that of environmental properties. The patterns of accumulated variance as a function of distance varied among the soil properties. The variability of SIR and DEA mainly occurred at small (1 m) scale (and at the 10-40 m-scales for SIR), probably reflecting the quality of litter input that results of the influence of local assemblage of different tree species, though changes in the soil N and C contents. Indeed, total soil C and N contents explained the microbial properties at every scale. Coefficients of codispersion showed that neither SOMQ nor IP did correlate with SIR and DEA, and confirmed that total C and N contents explained microbial properties in a scale dependent and complex manner. Such spatial dependency underlines the importance of soil heterogeneity in this tropical forest with implications for sampling strategies when studying the microbial processes and their response to disturbances. (C) 2012 Elsevier B.V. All rights reserved.
ISSN: 0016-7061

hal-01032409
https://hal.archives-ouvertes.fr/hal-01032409
DOI : 10.1016/j.geoderma.2012.12.009

Éditeur(s) : HAL CCSD Elsevier
Résumé : In the context of an ongoing monitoring study on the impacts of gold-mining activities on critical ecosystem processes, we explored the use of soil Denitrifying Enzyme Activity (DEA) and Substrate Induced Respiration (SIR) as ecosystem indicators in tropical rainforests of French Guiana. We also propose DEA/SIR ratio as ecosystem attribute able to describe the state of an ecosystem and to reflect changes in ecological processes. With this purpose, we measured SIR, DEA and DEA/SIR ratio in five gold-mining areas and five surrounding natural reference rainforests. We also measured indicators in two conditions of spontaneous regeneration of vegetation (stratified or not) and two conditions of soil rehabilitation (prior preparation of soils or not). We showed a high variability of DEA, SIR and DEA/SIR ratio in the natural reference forests. This pointed out the necessity to identify relevant reference systems - i.e. proving a close match in all relevant ecological dimensions - to compare with closed perturbed systems in order to assess the levels of alterations after disturbances. Results showed a high impact of gold mine on microbial processes with a strong decrease of DEA (10-fold lower), SIR (2-fold lower) and DEA/SIR ratio (8-fold lower) in perturbed areas in comparison with natural reference forests. The type of spontaneous vegetation (stratified or not) influenced the values of indicators as well as prior rehabilitation of soils, demonstrating the capacity of DEA. SIR and DEA/SIR ratio to respond in proportion to the perturbation (robustness) and to the different levels of restoration (sensitivity). The systematic decrease of the ratio DEA/SIR observed in the studied perturbed situations demonstrates clearly that the structure of microbial communities has been also modified. The ratio DEA/SIR proved to be robust and sensitive, and able to describe in fairly fine way changes of soil microbial communities in terms of structure and function in gold mine areas and during processes of restoration. We propose to use DEA, SIR and DEA/SIR ratio as bioindicators of both structural and functional aspects of C and N cycling in soils. Together with others bioindicators based on key supporting functions in soils, these indicators should accurately evaluate the ecological potential of natural ecosystems and the levels of degradation in case of land-use changes. (C) 2012 Elsevier Ltd. All rights reserved.
ISSN: 1470-160X

hal-01190730
https://hal.archives-ouvertes.fr/hal-01190730
DOI : 10.1016/j.ecolind.2012.01.021
PRODINRA : 213639

Éditeur(s) : HAL CCSD
Résumé :   Diffusion du document : INRA Antilles-Guyane, UMR ECOFOG, F97387 Kourou, Guyane (France) Diplôme : Master Recherche
il s'agit d'un type de produit dont les métadonnées ne correspondent pas aux métadonnées attendues dans les autres types de produit : DISSERTATION
Worldwide, there is concern regarding the declining quality of ecosystems, their rehabilitation, and their protection. In particular, the recognition that soil is a finite resource has spurred efforts to manage and restore it. In French Guiana, gold mining is a serious social and ecological problem, including the threats it poses to soil quality. The principal goal of this study was to establish a qualitative baseline of impacts of gold mining on soil microbial activity at five previously mined sites. We measured the potential rates of respiration and denitrification in disturbed soils, and compared theses rates with those of soils from adjacent forests, which served as un-disturbed references. Microbial activity, used as a bio-indicator, demonstrated the importance of soil rehabilitation, and the type of soil rehabilitation. We also found that visual observations of plant regeneration were insufficient to infer the rate of microbial activity. These bio-indicators can indicate soil quality, and help to formulate hypotheses about its developmnent. Bio-indicators can be used to obtain reliable indications about the quality of soil and contribute to decision-making in the management and restoration of this valuable resource.
Notre étude s'inscrit dans un contexte mondial de préoccupations environnementales, centrées notamment sur l'évaluation de la qualité des écosystèmes, la mise en place de leur réhabilitation et de leur sauvegarde. En particulier, la prise de conscience que le sol ne constitue pas une ressource inépuisable s'est accompagnée de la nécessité de gérer, voire de restaurer cette ressource. L'étude s'intègre également dans un contexte guyanais où l'exploitation aurifère constitue un problème écologique et sociétal majeur. Notre travail a pour but principal de dresser un bilan qualitatif des impacts de l'exploitation aurifère sur le fonctionnement microbien des sols de cinq sites miniers. Pour cela, nous avons effectué les mesures des activités potentielles de deux fonctions microbiennes, la respiration et la dénitrification, sur les sols des sites perturbés. Nous avons comparé ces valeurs d'activités microbiennes avec celles obtenues pour les sols de forêts naturelles (adjacentes à ces sites) que nous avons considérées comme la référence à atteindre (témoin). Les fonctions microbiennes, utilisées comme bio-indicateurs, ont mis en évidence l'importance de la réhabilitation et du type de réhabilitation sur la reprise du fonctionnement du sol. Nous avons constaté que l'observation de visu de la végétation ne suffit pas à évaluer de façon certaine la reprise de ce fonctionnement. Ces bio-indicateurs sont aptes à traduire l'état d'un sol perturbé et émettre des hypothèses quant à son évolution. Ainsi, les bio-indicateurs peuvent être utilisés afin d'obtenir des indications solides sur la qualité des sols et aider à la prise de décision en matière de gestion et de restauration de cette ressource.
https://hal.archives-ouvertes.fr/hal-01189544

hal-01189544
https://hal.archives-ouvertes.fr/hal-01189544
PRODINRA : 46710

Éditeur(s) : HAL CCSD Elsevier
Résumé :  
Microbial-based indicators, such as C and N contents or microbial functions involved in C and N cycles, are currently used to describe the status of soils in disturbed areas. Microbial functions are more accurate indicators but their measurement for studies at the ecosystem level remains problematical because of the huge spatial variability of these processes and, consequently, of the large number of soil samples which must be analyzed. Our goal was to test the capacity of near-infrared reflectance spectroscopy (NIRS) to predict respiration and denitrification but also carbon and nitrogen contents of soils submitted to various procedures of restoration. To achieve this objective, we took advantage of an experiment conducted on a reforestation system established after open-cast gold mining in French Guiana. In this experimental station, plantations of various ages and various soil textures were at our disposal. Our results showed that both plantations and soil texture had a strong impact on the recovery of soil functioning: carbon and nitrogen contents, respiration and denitrification increased with age of plantation and clay content. Calibrations were performed between spectral data and microbial-based indicators using partial least squares regression (PLS). The results showed that C and N contents were accurately predicted. Microbial functions were less precisely predicted with results more accurate on clayey soils than on sandy soils. In clayey soils, perturbed or restored soils and the year of plantation were discriminated very efficiently through principal component analyses of spectral signatures (over 80% of variance explained on the first two axes). Near-infrared spectroscopy may thus be extended to the prediction of functional soil parameters, but the capacity of this method must be strengthened by expending the databases with other soils in other contexts. The possibility of using NIRS provides many opportunities for understanding both the temporal dynamics and the spatial variability of the recovery of key microbial functions during soil restoration.
ISSN: 0929-1393

hal-01032188
https://hal.archives-ouvertes.fr/hal-01032188
DOI : 10.1016/j.apsoil.2007.07.001

Éditeur(s) : HAL CCSD Elsevier
Résumé :  
Previous studies in lowland tropical rainforests of French Guiana showed that, among non-N2-fixing trees, two groups of late successional species contrasting in their leaf 15N natural abundance coexist, suggesting two different main ways of nitrogen acquisition. Two abundant late-successional species typically co-occurring in rainforests in French Guiana, namely Eperua falcata and Dicorynia guianensis, were chosen as representative of each group. Stable isotope techniques and measurements of potentials of microbial N transformation were performed to assess to what extent leaf 15N natural abundance of these species could be related to (i) d15N signatures of soil mineral N sources and (ii) the capacity of soil to express nitrification and denitrification (both processes being directly involved in the balance between NH4 + and NO3 - ). Soil d15N-NH4 + was roughly similar to leaf d15N of D. guianensis (around 3.5%), suggesting a preferential use of NH4 +, whereas in E. falcata, leaf d15N values were closer to root d15N-NO3 _ values (0.2 and _2.0%, respectively), suggesting a preferential use of NO3 _. These differences in N source utilization were not accompanied by differences in availability in soil NO3 _ or in intensity of microbial functions responsible for soil N mineral evolution. However, (i) under both tree species, these functions showed clear spatial partitioning, with denitrification occurring potentially in soil and nitrification in the litter layer, and (ii) E. falcata fine roots colonized the litter layer much more strongly than D. guianensis fine roots. This strongly suggests that (i) the contrasted leaf d15N values found in the two late-successional species reveal distinct N acquisition strategies and (ii) the ability of roots to predominantly exploit the litter layer (E. falcata) or the soil (D. guianensis) may constitute an important explanation of the observed differences. A complementarity between tree species, based on mineral N resource partitioning (itself resulting from a spatially structured location of the microbial functions responsible for the balance between NH4 + and NO3 - ), can thus be supposed.
ISSN: 0038-0717

hal-01032035
https://hal.archives-ouvertes.fr/hal-01032035
DOI : 10.1016/j.soilbio.2007.09.011

Éditeur(s) : HAL CCSD
Résumé : We present high-resolution bulk sedimentary δ 15N data from the southern edge of the present-day oxygen minimum zone of the eastern South Pacific. The record is interpreted as representing changes in water column nitrogen removal during the last 70,000 years. We found significant fluctuations in the isotopic signal that suggest major reorganizations of the oxygen minimum zone at millennial timescales. These fluctuations were not related to other millennial-scale changes like the Northern Hemisphere's Dansgaard-Oeschger climate swings or local changes in primary productivity, so appear to be dictated by the Southern Hemisphere's climate rhythm. This is preliminarily corroborated by an overall agreement between our δ 15N data and the sedimentary proxy of ice sheet dynamics in Patagonia, which is in turn correlated with surface water properties at the midlatitude subduction region of the eastern South Pacific intermediate waters. Finally, potential implications on late Quaternary changes in atmospheric CO2 concentrations are discussed.
Paleoceanography / PALEOECEANOGRAPHY

hal-00407260
https://hal.archives-ouvertes.fr/hal-00407260
DOI : 10.1029/2006PA001308

Résumé : Une expérience a été réalisée en conditions hivernales dans six bassins de terre pour déterminer l'effet des taux de renouvellement sur la production de crevettes Penaeus stylirostris, sur les caractéristiques de l'eau et du sédiment des enceintes d'élevage. Les budgets d'azote ont été établis en prenant en compte les différentes formes azotées dans l'eau, dans le sédiment, dans l'aliment et dans les crevettes. Un épisode de mortalité a été obserVé en cours d'élevage et peut être attribué à une epizootie dénommée "syndrome 93». Ce dernier était concomitant à une phase de croissance phytoplantonique et a été d'autant plus important que la biomasse phytoplanctonique dans le bassin d'élevage était élevée. Bien qu'une dilution importante de la colonne d'eau se soit avérée bénéfique pour la santé du cheptel pendant l'épisode de mortalité, l'augmentation des taux de renouvellement n'a en revanche pas permis d'améliorer de manière significative la qualité de l'environnement crevette sur toute la saison d'élevage (eau et sédiment). Une augmentation des échanges d'eau ne s'est pas accompagnée d'une meilleure exportation des déchets par les effluents. Cependant, les résultats montrent que cette augmentation accroît la productivité primaire. Les composés issus de la minéralisation de la matière organique et du métabolisme sont ainsi exportés sous forme particulaire plutôt que sous forme soluble. Les budgets d'azote montrent que la quantité de déchets rejetés par les effluents vers l'environnement côtier est faible. Ainsi, 10,1% à 16,90/o de l'azote introduit dans le milieu d'élevage sous forme d'aliment est exporté dans les effluents. Seulement 17,3% à 21,4% de l'azote d'origine alimentaire est retrouvé dans la chair des crevettes. L'augmentation des échanges d'eau implique une augmentation de l'accumulation azotée dans les sédiments de 21,0% à 50,1%. Parallèlement, le calcul de la proportion d'azote évacué par diffusion ou par dénitrification vers l'atmosphère diminue de 43,1% à 19,5% avec l'augmentation des taux de renouvellement.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00079/19066/16661.pdf
http://archimer.ifremer.fr/doc/00079/19066/

Éditeur(s) : HAL CCSD
Résumé :   Diplôme : DEA
il s'agit d'un type de produit dont les métadonnées ne correspondent pas aux métadonnées attendues dans les autres types de produit : DISSERTATION
As part of a programme of degraded soils rehabilitation, and in order to follow the biological processes connected with the soils reconstitution, it is a requisite to determine indicators able to account for the evolution of the soil. These indicators can he found on structural and/or functional characteristics of the bacterial communities, such as soil respiration, denitrification or genetic diversity. Theses descriptors are able to characterize the global community or targeted communities chosen for their importance in the ecosystem functioning. In this work, we tried to know whether soil bacteria genetic diversity was able to be a relevant indicator of the soil evolution in a rehabilitated sequence. This diversity is apprehended via a molecular technique: ARISA. A chronosequence situated on a gold mine, already experimented as part of a programme of degraded zones rehabilitation, was at our disposal. Results show that ARISA is a very sensitive technique, able to account for the bacterial community heterogeneity in a soil but unable, at the parcel scale, to discriminate the treatments nor to account for the evolution in lime of planted treatments. Lastly, the advantage of this indicator of genetic diversity may arise from its confrontation to other indicators (functional, for example).
Dans le cadre de la revégétalisation d'un sol dégradé et afin de pouvoir suivre les processus biologiques liés à la reconstitution des sols, il est nécessaire de déterminer des indicateurs pouvant rendre compte de l'évolution de l'état du sol. Ces indicateurs peuvent être fondés sur des caractéristiques fonctionnelles et/ou structurales des communautés bactériennes telles que la respiration, la dénitrification ou la diversité génétique. Ces descripteurs peuvent analyser la communauté globale ou des communautés cibles choisies pour leur importance dans le fonctionnement de l'écosystème. L'objectif de ce stage était de savoir si la diversité génétique des bactéries du sol pouvait être un indicateur de l'évolution des sols au sein d'une séquence de revégétalisation. Cette diversité est appréhendée par une technique de biologie moléculaire : l'ARISA. On dispose d'une chronoséquence située sur une mine d'or étudiée dans le cadre d'un programme de réhabilitation des zones dégradées. Les résultats montrent que l'ARISA est une technique très sensible, qui rend bien compte de l’hétérogénéité des communautés bactériennes présentes dans un sol mais est incapable, à l’échelle de la parcelle, de discriminer ni les traitements ni de rendre compte de l'évolution dans le temps de ces parcelles. Enfin, l'intérêt de cet indicateur de diversité génétique pourra résulter de sa confrontation avec d'autres indicateurs (fonctionnels par exemple).
https://hal.archives-ouvertes.fr/hal-01189155

hal-01189155
https://hal.archives-ouvertes.fr/hal-01189155
PRODINRA : 9464