Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Spermmotility notably depends on the structural integrity of the flagellum and the regulation ofmicrotubule dynamics. Although researchers have started to use " omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined. In this study, we defined a core set of 116 gene products associated with the human sperm microtubulome (including products potentially involved in abnormal ciliary phenotypes and male infertility disorders). To this end, we designed and applied an integrated genomics workflow and combined relevant proteomics, transcriptomics, and interactomics datasets to reconstruct a dynamic interactome map. By further integrating phenotypic information, we defined a disease-interaction network; this enabled us to highlight a number of novel factors potentially associated with altered sperm motility and male fertility. Lastly, we experimentally validated the expression pattern of two candidate genes (CUL3 and DCDC2C) that had never previously been associated with male germline differentiation. Our analysis suggested that CUL3 and DCDC2C's products have important roles in the sperm flagellum. Taken as a whole, our results demonstrate that an integrated genomics strategy can highlight relevant molecular factors in specific sperm components. This approach could be easily extended by including other " omics" data (from asthenozoospermic men, for example) and identifying other critical proteins from the human sperm microtubulome. Summary Sentence Although researchers have started to use "omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined.
ISSN: 0006-3363

hal-01515563
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01515563
DOI : 10.1095/biolreprod.116.143479
PUBMED : 28395323

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Analysis of short-term deformation along the southern part of Dead Sea Fault (DSF) provides a systematic view of kinematics this part of the continental transform. The southern DSF consists of two principal segments: the Wadi Araba and Jordan Valley faults. In addition to other regional continuous GPS data, this study uses new data from 25 survey sites and 4 continuous GPS stations in Jordan for improved near-field observations. Resulting velocities are reported with 1-sigma uncertainties ranging from 0.4-1.0 mm/yr. Application of elastic dislocation models yields estimates of slip rates for Wadi Araba and Jordan Valley faults are 4.9 +/- 0.4 mm/yr and 4.7 +/- 0.4 mm/yr, respectively. Modeling also suggests different depths of effective fault locking with 15 +/- 5 km and 8 +/- 5 km for the Wadi Araba and Jordan Valley faults, respectively. These slip rates are generally consistent with the upper end of the range of slip rates estimated from late Quaternary geology. Spatial variations in effective fault locking generally correspond with a heterogeneous mantle lithosphere. A similar observation can be observed along the southern San Andreas Fault, and this may reflect the influence of heterogeneity in the uppermost mantle on crustal faulting processes.
ISSN: 1525-2027

hal-00689031
https://hal.archives-ouvertes.fr/hal-00689031
DOI : 10.1029/2011GC003736