Éditeur(s) : Estuaries Research Federation
Résumé : Estuarine systems are complex environments where seasonal and spatial variations occur in concentrations of suspended particulate matter, in primary constituents, and in organic matter content. This study investigated in the laboratory the flocculation potential of estuarine-suspended particulate matter throughout the year in order to better identify the controlling factors and their hierarchy. Kinetic experiments were performed in the lab with a "video in lab" device, based on a jar test technique, using suspended sediments sampled every 2 months over a 14-month period at three stations in the Seine estuary (France). These sampling stations are representative of (1) the upper estuary, dominated by freshwater, and (2) the middle estuary, characterized by a strong salinity gradient and the presence of an estuarine turbidity maximum. Experiments were performed at a constant low turbulent shear stress characteristic of slack water periods (i.e., a Kolmogorov microscale > 1,000 A mu m). Flocculation processes were estimated using three parameters: flocculation efficiency, flocculation speed, and flocculation time. Results showed that the flocculation that occurred at the three stations was mainly influenced by the concentration of the suspended particulate matter: maximum floc size was observed for concentrations above 0.1 g l(-1) while no flocculation was observed for concentrations below 0.004 g l(-1). Diatom blooms strongly enhanced flocculation speed and, to a lesser extent, flocculation efficiency. During this period, the maximum flocculation speed of 6 A mu m min(-1) corresponded to a flocculation time of less than 20 min. Salinity did not appear to automatically enhance flocculation, which depended on the constituents of suspended sediments and on the content and concentration of organic matter. Examination of the variability of 2D fractal dimension during flocculation experiments revealed restructuring of flocs during aggregation. This was observed as a rapid decrease in the floc fractal dimension from 2 to 1.4 during the first minutes of the flocculation stage, followed by a slight increase up to 1.8. Deflocculation experiments enabled determination of the influence of turbulent structures on flocculation processes and confirmed that turbulent intensity is one of the main determining factors of maximum floc size.
Estuaries and coasts : Journal of the Estuarine Research Federation (1559-2723) (Estuaries Research Federation), 2009-07 , Vol. 32 , N. 4 , P. 678-693

Droits : 2009 Springer
http://archimer.ifremer.fr/doc/2009/publication-6631.pdf
DOI:10.1007/s12237-009-9160-1
http://archimer.ifremer.fr/doc/00000/6631/

Éditeur(s) : HAL CCSD
Résumé : National audience
Le littoral, caractérisé par une grande diversitéd’environnements (plages sableuses ou rocheuses, cordonsdunaires, falaises, lagunes, estuaires...) est une zone très mobile etévolutive du fait de forçages hydrodynamiques et météorologiquessouvent intenses. Cet environnement est fragile, car soumis àl’impact d’activités humaines qui ne cessent d’augmenter (urbanisation,aménagements...), mais aussi aux effets du changementclimatique (élévation du niveau de la mer, modification durégime de houles, inondations). Comprendre le fonctionnementhydromorpho-sédimentaire des environnements littoraux est crucialpour prédire leur évolution et celle des écosystèmes associés.Le Service National d’Observation labellisé par le CNRS, (SNO)DYNALIT, - Dynamique du Littoral et du Trait de Côte a pourbut de constituer une base de données hydromorphologiques sur lelong terme de 29 sites ateliers représentatifs des environnementslittoraux français (côtes sableuses, falaises et embouchures) afin :- De mesurer la cinématique de ces environnements et du trait decôte associé ;- De mieux comprendre les interactions entre les processushydrodynamiques-transport sédimentaires-réponse morphologiqueassociés à la dynamique du littoral ;- Utiliser les observations pour améliorer notre connaissance desréponses de la morphologie des littoraux dans différents contextesde forçages météo-marins ;- D’utiliser ces observations pour analyser l’importance relativedes facteurs anthropiques et naturels sur la dynamique littorale ;- De se positionner en interlocuteur pour les responsables de ladéfinition et la mise en oeuvre des politiques de gestion des risqueslittoraux.
RST 2016, livre des résumés
Caen, France

insu-01387873
https://hal-insu.archives-ouvertes.fr/insu-01387873

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The parasitic life cycle of Leishmania includes an extracellular promastigote stage that occurs in the gut of the insect vector. During that period, the sucrose metabolism and more specifically the first glycosidase of this pathway are essential for growth and survival of the parasite. We investigated the expression of the invertase BfrA in the promastigote and amastigote stages of three parasite species representative of the three various clinical forms and of various geographical areas, namely Leishmania major, L. donovani and L. braziliensis. Thereafter, we cloned, overexpressed and biochemically characterized this invertase BfrA from L. major, heterologously expressed in both Escherichia coli and L. tarentolae. For all species, expression levels of BfrA mRNA were correlated to the time of the culture and the parasitic stage (promastigotes > amastigotes). BfrA exhibited no activity when expressed as a glycoprotein in L. tarentolae but proved to be an invertase when not glycosylated, yet owing low sequence homology with other invertases from the same family. Our data suggest that BfrA is an original invertase that is located inside the parasite. It is expressed in both parasitic stages, though to a higher extent in promastigotes. This work provides new insight into the parasite sucrose metabolism
ISSN: 0008-6215

hal-01187142
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187142
DOI : 10.1016/j.carres.2015.07.001
PUBMED : 26279524