Extrait de : Les bagnes : Histoire, Types, Mœurs, Mystères (p. 384)
Résumé : Illustration représentant une fanfare de forçats au bagne de Toulon
Siècle(s) traité(s) : 19
Droits : Domaine public
Permalien : http://www.manioc.org/images/FRA110250436i1
FRA110250436i1

Extrait de : Deux années au Brésil (P.19)
Résumé : Vignette montrant des musiciens allemands à bord du steamer The Tyne faisant route vers le Brésil
Siècle(s) traité(s) : 19
Droits : Domaine public
Provenance : Université des Antilles et de la Guyane. Service commun de la documentation
Permalien : http://www.manioc.org/images/HASH62c92253fe6630cd687e6f
HASH62c92253fe6630cd687e6f

Extrait de : "Madinina ""Reine des Antilles""" (En regard de la page 29)
Résumé : Cliché
Siècle(s) traité(s) : 20
Droits : Domaine public
Permalien : http://www.manioc.org/images/PAP110650051i1
PAP110650051i1

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Steel pan music, which evolved from African drum music, originated in Trinidad and Tobago in the early 20th century and is often a featured instrument during Carnival. As one of the national emblems of Trinidad and Tobago, steel pans were traditionally made from used steel oil drums or steel containers. The container’s head is concave and the playing surface is divided into convex sections by slants to make the pan's range of sounds. In this image are used steel oil drums, some steel pans with the Shell Oil Company logo and the word "Invaders," painted white metal steel pan stands, and breadfruit trees. One poster is advertising the Shell Invaders Steel Orchestra. Other signs includes phrases such as: "We are the greatest" and "Notice there is to be no unnecessary coasting in the yard." In the background is a woman standing on top of a concrete building's roof with laudry and men standing near the wall. Slide labeled Trin. 16.
Trinidad and Tobago -- South America -- Trinidad
Trinidad and Tobago -- Caribbean region -- Trinidad

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
http://ufdc.ufl.edu/AA00031210/00001

Résumé : Caption on back of photograph: "The new year finds Havana much gayer than it has been for the past few years. Vacation throngs are arriving daily by passenger ships and airplanes. Bathing beaches are crowded, and yachting, fishing, hunting and racing are attracting enthusiasts. Social activities are increasing. Spanish and Cuban clubs have arranged special siestas. The Cuban Industrial and Agricultural Exhibition at Rancho Boyerso, just outside of Havana, will attract many visitors. Will P. Taylor, Manager of the National Hotel of Cuba, reports a larger number of reservations than anytime during the past five years. A well known orchestra is under the direction of Sr. Armando Romeu, Jr. The daily tea and dinner dances are very popular, All in all, the National Hotel of Cuba looks forward to the most active season in its history. All rights reserved by the source institution. Shows the front facade and landscaped grounds of the hotel.
(Biographical) The National Hotel was designed in 1930 by the New York architectural firm of McKim, Mead & White.
(Preferred Citation) Cite as Miami News Collection, HistoryMiami,1989-011-4325.
Havana, Cuba

Droits : All rights reserved by the source institution.
1989-011-4325
http://ufdc.ufl.edu/AA00015711/00001

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Two steel pan instrument makers are hammering the surfaces of drums to make the range of sound for steel pan instruments. One of the men is hammering a white steel drum, while the other is in the distance near red steel drums. These particular steel pans are made from used steel oil drums. The steel pan, a percussion instrument, originated in Trinidad and Tobago and is one of the country’s national emblems. In the yard are other used steel oil drums, white metal steel pan stands, and many breadfruit trees. In the background are buildings, some made of wood with zinc paneled roofs. There is also a poster advertising the Shell Invaders Steel Orchestra, a steel band active during the 1960s and sponsored by Shell Oil Company. Some of the notices in the yard read as follows: "Notice don't sit stand lean or prop on the stands," "We are the greatest," "Notice absolutely no unnecessary coasting in the yard." Slide labeled Trin. 17
Trinidad and Tobago -- South America -- Trinidad
Trinidad and Tobago -- Caribbean region -- Trinidad

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
http://ufdc.ufl.edu/AA00031211/00001

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : Université de Guyane
Extrait de : "Bals masqués de Guyane et d'ailleurs. Identités et imaginaires carnavalesques en question" : colloque international, les 26 et 27 janvier 2017. Université de Guyane
Description : Le bal paré masqué ou le personnage emblématique du touloulou émerge le temps d'une saison et anime la vie sociétale l'imaginaire guyanais. Il est le point de mire au niveau de certaine conversation, des devantures de magasins et au niveau dans le milieu des bals masqués revêt l'habit d'apparat qui évolue au fil des temps. Ce personnage de touloulou aux multiples attraits et cela dans l'optique de converger tous les regards vers lui. Le touloulou, lui, entend régenter à sa guise les espaces dédiés à la danse. Le carnaval est sujet à de nombreuses interprétations et inspire de nombreux écrivains. Le bal paré masqué guyanais dans "le bois roman du serpent noir", (Bernard Blasse, 1980) est entrevu comme une délivrance, le signe d'une renaissance pour la cité et dès lors, tout s?orchestre avec frénésie, autour de cet événement mémorable. Le carnaval et en l?occurrence le bal paré est pour certain, un monde commun et imaginé des possibles un monde ardent et convulsé autour d'un personnage phare, éphémère, mais qu'en est-il somme toute, des lendemains festifs quand les masques tombent et l'humain lui, renvoyé à son quotidien ?
Siècle(s) traité(s) : 20
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V17165
V17165

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
: Until recently, synonymous mutations (which do not change amino acids) have been much neglected. Some evidence suggests that this kind of mutations could affect mRNA secondary structure or stability, translation kinetics and protein structure. To explore deeper the role of synonymous mutations, we studied their consequence on the functional activity of the estrogen receptor alpha (ERα). The ERα is a ligand-inducible transcription factor that orchestrates pleiotropic cellular effects, at both genomic and non-genomic levels in response to estrogens. In this work we analyzed in transient transfection experiments, the activity of ERα carrying the synonymous mutation Ala87, a polymorphism involving about 5-10% of the population. In comparison to the wild type receptor, our results show that ERαA87 mutation reduces the transactivation efficiency of ERα on an ERE reporter gene while its expression level remains similar. This mutation enhances 4-OHT-induced transactivation of ERα on an AP1 reporter gene. Finally, the mutation affects the subcellular localization of ERα in a cell type specific manner. It enhances the cytoplasmic location of ERα without significant changes in non-genomic effects of E2. The functional alteration of the ERαA87 determined in this work highlights the relevance of synonymous mutations for biomedical and pharmacological points of view.
Journal of steroid biochemistry and molecular biology

inserm-01015532
http://www.hal.inserm.fr/inserm-01015532
DOI : 10.1016/j.jsbmb.2014.02.016
PUBMED : 24607813

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Interleukin-27 (IL-27) belongs to the IL-6/IL-12 family of cytokines, associated with different inflammatory diseases and orchestrates its biological activity via common heterodimeric receptor composed of WSX-1 (IL-27Rα) and gp130. The present study was aimed to investigate the regulation of CXCL9, CXCL10, and CXCL11 chemokines in hepatic cells (human LX-2 cell line derived from normal human stellate cells (HSC), primary human hepatocytes, HSC, and HepG2 cells) and concanavalin A (ConA)-induced liver inflammation. We demonstrated that IL-27, but not IL-6, induced/up-regulated CXCR3 ligand genes (CXCL9, CXCL10, and CXCL11; out of 26 selected genes) in a STAT1-dependent manner in hepatic cells in vitro both at transcript and protein levels. In ConA-induced T cell-mediated hepatic model, we showed that soluble IL-27/IFNγ was elevated following ConA hepatitis in association with increased CXCL9, CXCL10, and CXCL11 expression in the liver. The exogenous IL-27 administration induced CXCR3 ligands in mouse liver at 4 h with any significant effect on recruitment of CXCR3(+) immune cells in the liver. The neutralization of IL-27 during ConA hepatitis differentially modulated (transcript vs protein expression) CXCR3 ligands and IFNγ during ConA-induced hepatitis with down-regulated expression of CXCL9 and CXCL10 at transcript level. The IFNγ, complementary regulated the expression of CXCR3 ligands as their up-regulation during ConA hepatitis, was abolished in IFNγ KO mice. In summary, IL-27 up-regulated the CXCL9, CXCL10, and CXCL11 chemokine expression in hepatic cells. IL-27 regulated CXCR3 ligand expression in IFNγ-dependent manner during acute hepatitis suggesting a complementary role of IL-27 and IFNγ to moderate liver inflammation via regulation of CXCR3 ligands. KEY MESSAGE: IL-27 up-regulated CXCR3 ligand expression in human hepatic cells in vitro. IL-27 up-regulated CXCR3 ligand expression and secretion in ConA hepatitis in vivo. CXCR3 ligand expression was down-regulated by blocking IL-27 or IFNγ deficiency. IL-27 modulated liver injury by regulation of CXCR3 ligands in IFNγ-dependent manner.
ISSN: 0946-2716

hal-01214006
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01214006
DOI : 10.1007/s00109-015-1319-6
PUBMED : 26199110

Éditeur(s) : HAL CCSD Peter Lang
Résumé : International audience
https://hal.archives-ouvertes.fr/hal-01151982

hal-01151982
https://hal.archives-ouvertes.fr/hal-01151982

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na+/H+ exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways.
ISSN: 2045-2322

hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/file/Cleaved%20FAS%20ligand%20CC-BY.pdf
DOI : 10.1038/srep28008

Éditeur(s) : HAL CCSD
Résumé : Half-way between optimization and astronautic, this research study deals with satellites constel- lations design problems. In order to find the required number of satellites and to correctly set their position, such are the technical challenges of this thesis. To minimise the cost and reduce the time such are the economical challenges the space industries are confronted in a daily manner. The major difficulties of these problems are the size and the characteristics of the search space The irregularity of the criterions The mathematical and physical heterogeneity of parameters forbids the use of classical algorithms The evaluation of a solution, which uses a time consuming evalua- tion without returning pertinent information about the good or bad properties of the constellations, forbid a massive exploration of the search space. From this constatation and several preliminary studies a new approach is born. Based on a better use of the simulation and on a simplification of the criterions, the algorithm is composed of several levels and uses different optimization techniques it integrates a knowledge database on the orbits and a numerical optimization process both orchestrated by a metaheuristic algorithm. This new approach tries to bypass the main drawbacks of the field with a decomposition of the problem.
A mi-chemin entre l optimisation et l astronautique ce travail de recherche s attaque au problème du design de constellations de satellites Déterminer le nombre de satellites nécessaires pour ré- pondre à un besoin et les positionner de manière correcte tels sont les enjeux techniques de cette thèse Minimiser les coûts et les délais tels sont les enjeux économiques auxquels est confronté le domaine spatial au quotidien Les difficultés majeures de ce problème sont d une part la taille et les caractéristiques de l espace de recherche; D autre part la non-régularité des critères utilisés et la nature mathématique et phy- sique hétérogène de l ensemble des paramètres (paramètres entiers et paramètres réels) interdisant l utilisation d algorithmes classiques; Enfin l évaluation des solutions qui met en oeuvre une simu- lation coûteuse en temps et en calculs sans retourner d information pertinente sur les bonnes ou mauvaises propriétés de la constellation évaluée interdit une exploration massive de l espace de recherche De ces constatations et de diverses études préliminaires est née une nouvelle approche Basé sur une meilleure exploitation de la simulation et sur une simplification des critères utilisés l algorithme composé de plusieurs niveaux met en oeuvre plusieurs techniques d optimisation En intégrant une base de connaissance sur les orbites et une méthode numérique de recherche d optimum le tout or- chestré par un algorithme métaheuristique cette nouvelle approche tente de résoudre les difficultés intrinsèques au domaine d application en décomposant le problème.
https://tel.archives-ouvertes.fr/tel-00634769

tel-00634769
https://tel.archives-ouvertes.fr/tel-00634769
https://tel.archives-ouvertes.fr/tel-00634769/document
https://tel.archives-ouvertes.fr/tel-00634769/file/grandchamp_phd_2001.pdf

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Mammalian spermatogenesis is a complex and highly orchestrated combination of processes in which male germline proliferation and differentiation result in the production of mature spermatozoa. If recent genome-wide studies have contributed to the in-depth analysis of the male germline protein-encoding transcriptome, little effort has yet been devoted to the systematic identification of novel unannotated transcribed regions expressed during mammalian spermatogenesis. We report high-resolution expression profiling of male germ cells in rat, using next-generation sequencing technology and highly enriched testicular cell populations. Among 20 424 high-confidence transcripts reconstructed, we defined a stringent set of 1419 long multi-exonic unannotated transcripts expressed in the testis (testis-expressed unannotated transcripts [TUTs]). TUTs were divided into 7 groups with different expression patterns. Most TUTs share many of the characteristics of vertebrate long noncoding RNAs (lncRNAs). We also markedly reinforced the finding that TUTs and known lncRNAs accumulate during the meiotic and postmeiotic stages of spermatogenesis in mammals and that X-linked meiotic TUTs do not escape the silencing effects of meiotic sex chromosome inactivation. Importantly, we discovered that TUTs and known lncRNAs with a peak expression during meiosis define a distinct class of noncoding transcripts that exhibit exons twice as long as those of other transcripts. Our study provides new insights in transcriptional profiling of the male germline and represents a high-quality resource for novel loci expressed during spermatogenesis that significantly contributes to rat genome annotation.
ISSN: 0006-3363

hal-01063924
https://hal.archives-ouvertes.fr/hal-01063924
DOI : 10.1095/biolreprod.114.118166
PUBMED : 24740603