Éditeur(s) : Leslie's Weekly Leslie's Weekly ( New York )
Résumé : Page 173 from Leslie's Weekly, Nov,. Article entitled "The Kingston earthquake at short range", by Francis E. Clark, Founder of the United Society of Christian Endeavor continues on verso.
Six illustrations: Plum Point Lighthouse, which was damaged by the earthquake and its ligth extinguished, causing the stranding of the steamship "Prinz Waldemar". -- Refugees each receiving the meagre food supply of two potatoes, a piece of bread, some brown sugar, and molasses. -- The American Consulate a mass of ruins - Vice-Counsul William H. Orrett standing in front of his demolished office. -- Hundreds of refugees in camp a the temporary market near the Parade ground. -- A crowd of homeless and hungry people waiting for the opening of the gate of the race-course relief station. -- Curious incident of the quake - Queen Victoria's statue, once facing the sea, turned completely around. -- Steamship "Prinz Waldemar," of the Hamburg-American Line, wrecked on the Jamaica coast because the Plum Point Light went out. -- Entrance to Up Park Camp, headquarters of the First West Indian Regiment, showing wreckage of gateways and buildings.
Kingston (Jamaica)

Droits : All rights reserved by the source institution.
2005-348-1

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
This photograph shows the rope making process in greater detail. Once the strands are attached to the wooden, they are stretched out. As the man at the very end pulls and turns his end the girl holding the wooden panel in the middle makes sure that the rope is kept straight and does not get tangled. This process, which is common for rope making generally uses three or four strands that are twisted into rope with a hand operated rope machine such as the one pictured. The photograph also shows two men at the very end with the plank, a girl holding the wooden panel and a man taking a break under the shade of a nearby tree.
Cuba -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 84: 5
http://ufdc.ufl.edu/CA01200451/00001

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
The photograph shows one of the stages in the rope-making process. The girl in the center is holding a wooden panel that separates three strands of rope while the man at the end turns the handle that will twist the rope together.
Cuba -- Caribbean region -- Cuba -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 85: 1
http://ufdc.ufl.edu/CA01200467/00001

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
A man in the process of straightening out a hand made rope. He is holding between his legs and under his arms the strands of leaves that he will place in the rope as he twists it.
Cuba -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 84: 4
http://ufdc.ufl.edu/CA01200450/00001

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Processing rope in the Cuban countryside. Strands of rope being hung out before they are stretched out and intertwined in the final process of hand made rope making.
Cuba -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 84: 17
http://ufdc.ufl.edu/CA01200463/00001

Éditeur(s) : Springer
Résumé : Batch culture experiments using viral enrichment were conducted to test the response of a coastal bacterial community to autochthonous (i.e., co-existing) or allochthonous riverine viruses. The effects of viral infections on bacterial dynamics and activity were assessed by epifluorescence microscopy and thymidine incorporation, respectively, whereas the effect of viral infection on bacterial community composition was examined by polymerase chain reaction-single strand conformation polymorphism 16S ribosomal RNA fingerprinting. The percentages of high nucleic acid-containing cells, evaluated by flow cytometry, were significantly correlated (r (2) = 0.91, n = 12, p < 0.0001) to bacterial production, making this value a good predictor of active cell dynamics along the study. While confinement and temperature were the two principal experimental factors affecting bacterial community composition and dynamics, respectively, additions of freshwater viruses had significant effects on coastal bacterial communities. Thus, foreign viruses significantly reduced net bacterial population increase as compared to the enrichment treated with inactivated virus. Moreover, freshwater viruses recurrently and specifically affected bacterial community composition, as compared to addition of autochthonous viruses. In most cases, the combined treatment viruses and freshwater dissolved organic matter helped to maintain or even enhance species richness in coastal bacterial communities in agreement to the 'killing the winner' hypothesis. Thus, riverine virus input could potentially influence bacterial community composition of the coastal bay albeit with modest modification of bulk bacterial growth.
Microbial Ecology (0095-3628) (Springer), 2009-02 , Vol. 57 , N. 2 , P. 295-306

Droits : 2009 Springer
http://archimer.ifremer.fr/doc/2009/publication-6636.pdf
DOI:10.1007/s00248-008-9428-1
http://archimer.ifremer.fr/doc/00000/6636/

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
The aim of the study was to investigate the impact of first-line combined antiretroviral therapy (cART) regimen on the course of CD8 + T-cell counts in human immunodeficiency virus (HIV)-infected patients. A retrospective observational study conducted on the French DAT'AIDS Cohort of HIV-infected patients. We selected 605 patients initiating a first-line cART between 2002 and 2009, and which achieved a sustained undetectable HIV plasma viral load (pVL) for at least 12 months without cART modification. The evolution of CD8 + T-cell counts according to cART regimen was assessed. CD8 + T-cell counts were assessed in 572 patients treated with 2NRTIs+1PI/r (n= 297), 2NRTIs+1NNRTI (n= 207) and 3NRTIs (n= 68). In multivariate analysis, after 12 months of follow-up, the 3NRTIs regimen was associated with a significantly smaller decrease of CD8 + T-cell count compared with NNRTI-containing regimens (–10.2 cells/mL in 3NRTIs vs –105.1 cells/mL; P=0.02) but not compared with PI-containing regimens (10.2 vs –60.9 cells/mL; P=0.21). After 24 months, the 3NRTIs regimen was associated with a smaller decrease of CD8 + T-cell count and % compared with PI/r-and NNRTI-containing regimens (0.2 in 3NRTIs vs –9.9 with PI/r-regimens, P=0.001, and vs –11.1 with NNRTI-regimens, p < 0.0001). A focus analysis on 11 patients treated with an INSTI-containing cART regimen during the study period showed after 12 months of follow-up, a median decrease of CD8 + T-cell count of –155 [inter quartile range: –302; –22] cells/mL. Our data highlight the fact that cART regimens have differential effects on CD8 pool down regulation. Abbreviations: cART = combined antiretroviral therapy, HIV = human immunodeficiency virus, INSTI = integrase strand transfer inhibitor, NNRTI = non-nucleotide reverse transcriptase inhibitor, NRTI = nucleotide reverse transcriptase inhibitor, PI/r = Ritonavir-boosted protease inhibitor, pVL = plasma viral load.
ISSN: 0025-7974

inserm-01408002
http://www.hal.inserm.fr/inserm-01408002
http://www.hal.inserm.fr/inserm-01408002/document
http://www.hal.inserm.fr/inserm-01408002/file/2016%2C%20Poizot-Martin%20-%20First-line%20cART%20regimen%20impacts%20the%20course%20of%20CD8%2B%20T-cell%20counts%20in%20HIV-infected%20patients.pdf
DOI : 10.1097/MD.0000000000005087

Éditeur(s) : HAL CCSD American Association of Petroleum Geologists
Résumé : International audience
Determination of the membrane seal capacity of deformation bands is critical for managing geologic reservoirs in porous sandstones. In this study, we have analyzed a cataclastic shear-band network developed in uncemented porous sandstone in Provence, France. Geometrical analyses of the bands show significant differences between three types of bands (single strand, multistrand, and band cluster), sorted by their number of strands, their amount of shear displacement, and their thicknesses. At the microscopic scale, the image-analysis porosities and the grain-size distributions allow definition of three different types of microstructural deformation: damage zone, protocataclastic, and cataclastic. Whereas damage zone and protocataclastic deformations are observed in each type of band, cataclastic strands are observed in clusters and, sometimes, in multistrands. Cataclastic strands are characterized by a porosity reduction of 10 to 25% and a permeability reduction of three to five orders of magnitude compared to the host rock. Field observations of iron hydroxide precipitations around the bands suggest that cataclastic strands were membrane seals to water flow under vadose condition. This study therefore highlights the importance of the degree of cataclasis in shear bands as membrane seals to subsurface fluid flows in sandstone reservoirs.
ISSN: 0149-1423

hal-00766557
https://hal.archives-ouvertes.fr/hal-00766557
DOI : 10.1306/04051211097

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
New structural observations coupled with 15 U/Pb and 24 Ar/Ar new ages from the Karakorum shear zone (KSZ) constrain the timing and slip rate of the right-lateral Karakorum fault zone (KFZ), one of the great continental Asian strike-slip faults. In the Tangtse-Darbuk area, the Tangtse (SW) and Muglib (NE) mylonitic strands of the KSZ frame the less deformed Pangong Range. Inherited U/Pb ages show that granitic protoliths are mostly from the Karakorum and Ladakh batoliths, with a major Miocene melting event lasting from ≥21.5 to 13.5 Ma. Some of the Miocene granitic bodies show structural evidence for intrusion synkinematic to the KSZ. The oldest of these granitoids is 18.8 ± 0.4 Ma old, implying that deformation started prior to ∼19 Ma. Microstructural data show that right-lateral deformation pursued during cooling. Ar/Ar data show that ductile deformation stopped earlier in the Tangtse (∼11 Ma) than in the Muglib strand (∼7 Ma). Deformation ended at ∼11 Ma in the Tangtse strand while it is still active in the Muglib strand, suggesting a progressive localization of deformation. When merged with published observations along the KFZ, these data suggest that the KFZ nucleated in the North Ayilari range at least ∼22 Ma ago. The long-term fault rate is 0.84 to 1.3 cm/yr, considering a total offset of 200 to 240 km. The KSZ collected magma produced within the shear zone and/or deeper in crust for which the producing mechanism stays unclear but was not the lower crustal channel flow.
ISSN: 0278-7407

hal-00745066
https://hal.archives-ouvertes.fr/hal-00745066
DOI : 10.1029/2011TC003049

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Saccharomyces cerevisiae is an established model organism for the molecular analysis of fundamental biological processes. The genomes of numerous strains have been sequenced, and the transcriptome and proteome ofmajor phases during the haploid and diploid yeast life cycle have been determined. However, much less is known about dynamic changes of the proteome when cells switch from mitotic growth to meiotic development. We report a quantitative protein profiling analysis of yeast cell division and differentiation based on mass spectrometry. Information about protein levels was integrated with strand-specific tiling array expression data. We identified a total of 2366 proteins in at least one condition, including 175 proteins showing a statistically significant > 5-fold change across the sample set, and 136 proteins detectable in sporulating but not respiring cells. We correlate protein expression patterns with biological processes and molecular function by Gene Ontology term enrichment, chemoprofiling, transcription interference and the formation of double stranded RNAs by overlapping sense/antisense transcripts. Our work provides initial quantitative insight into protein expression in diploid respiring and differentiating yeast cells. Critically, it associates developmentally regulated induction of antisense long noncoding RNAs and double stranded RNAs with fluctuating protein concentrations during growth and development.
ISSN: 1874-3919

hal-01485858
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858/file/Becker%20et%20al.%20-%20The%20protein%20expression%20landscape%20of%20mitosis%20and%20me.pdf
DOI : 10.1016/j.jprot.2016.12.016

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
DNase I requires Ca²+ and Mg²+ for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca²+ while the other two sites coordinate Mg²+. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca²+ stabilizes the functional DNase I structure. The presence of Mg²+ in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.
ISSN: 1553-734X

hal-00645165
https://hal.archives-ouvertes.fr/hal-00645165
https://hal.archives-ouvertes.fr/hal-00645165/document
https://hal.archives-ouvertes.fr/hal-00645165/file/journal.pcbi.1001000.pdf
DOI : 10.1371/journal.pcbi.1001000

Éditeur(s) : HAL CCSD Cold Spring Harbor Laboratory Press
Résumé : International audience
Meiotic DNA double-stranded breaks (DSBs) initiate genetic recombination in discrete areas of the genome called recombination hotspots. DSBs can be directly mapped using chromatin immunoprecipitation followed by sequencing (ChIP-seq). Nevertheless, the genome-wide mapping of recombination hotspots in mammals is still a challenge due to the low frequency of recombination, high heterogeneity of the germ cell population, and the relatively low efficiency of ChIP. To overcome these limitations we have developed a novel method--single-stranded DNA (ssDNA) sequencing (SSDS)--that specifically detects protein-bound single-stranded DNA at DSB ends. SSDS comprises a computational framework for the specific detection of ssDNA-derived reads in a sequencing library and a new library preparation procedure for the enrichment of fragments originating from ssDNA. The use of our technique reduces the nonspecific double-stranded DNA (dsDNA) background >10-fold. Our method can be extended to other systems where the identification of ssDNA or DSBs is desired.
ISSN: 1088-9051

hal-00877675
https://hal.archives-ouvertes.fr/hal-00877675
DOI : 10.1101/gr.130583.111
PUBMED : 22367190

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
A young girl in the Cuban countryside standing behind a machine used to make rope. The rope is attached to the plank, while someone else stretches the ropes and twists the strands together.
Cuba -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 84: 20
http://ufdc.ufl.edu/CA01200466/00001

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Homologous recombination is the key process that generates genetic diversity and drives evolution. SPO11 protein triggers recombination by introducing DNA double stranded breaks at discreet areas of the genome called recombination hotspots. The hotspot locations are largely determined by the DNA binding specificity of the PRDM9 protein in human, mice and most other mammals. In budding yeast Saccharomyces cerevisae, which lacks a Prdm9 gene, meiotic breaks are formed opportunistically in the regions of accessible chromatin, primarily at gene promoters. The genome-wide distribution of hotspots in this organism can be altered by tethering Spo11 protein to Gal4 recognition sequences in the strain expressing Spo11 attached to the DNA binding domain of the Gal4 transcription factor. To establish whether similar re-targeting of meiotic breaks can be achieved in PRDM9-containing organisms we have generated a Gal4BD-Spo11 mouse that expresses SPO11 protein joined to the DNA binding domain of yeast Gal4. RESULTS: We have mapped the genome-wide distribution of the recombination initiation sites in the Gal4BD-Spo11 mice. More than two hundred of the hotspots in these mice were novel and were likely defined by Gal4BD, as the Gal4 consensus motif was clustered around the centers in these hotspots. Surprisingly, meiotic DNA breaks in the Gal4BD-Spo11 mice were significantly depleted near the ends of chromosomes. The effect is particularly striking at the pseudoautosomal region of the X and Y chromosomes -- normally the hottest region in the genome. CONCLUSIONS: Our data suggest that specific, yet-unidentified factors influence the initiation of meiotic recombination at subtelomeric chromosomal regions.
ISSN: 1471-2164

inserm-00849779
http://www.hal.inserm.fr/inserm-00849779
http://www.hal.inserm.fr/inserm-00849779/document
http://www.hal.inserm.fr/inserm-00849779/file/1471-2164-14-493.pdf
DOI : 10.1186/1471-2164-14-493

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Meiotic recombination predominantly occurs at discrete genomic loci called recombination hotspots, but the features defining these areas are still largely unknown (reviewed in refs 1-5). To allow a comprehensive analysis of hotspot-associated DNA and chromatin characteristics, we developed a direct molecular approach for mapping meiotic DNA double-strand breaks that initiate recombination. Here we present the genome-wide distribution of recombination initiation sites in the mouse genome. Hotspot centres are mapped with approximately 200-nucleotide precision, which allows analysis of the fine structural details of the preferred recombination sites. We determine that hotspots share a centrally distributed consensus motif, possess a nucleotide skew that changes polarity at the centres of hotspots and have an intrinsic preference to be occupied by a nucleosome. Furthermore, we find that the vast majority of recombination initiation sites in mouse males are associated with testis-specific trimethylation of lysine 4 on histone H3 that is distinct from histone H3 lysine 4 trimethylation marks associated with transcription. The recombination map presented here has been derived from a homogeneous mouse population with a defined genetic background and therefore lends itself to extensive future experimental exploration. We note that the mapping technique developed here does not depend on the availability of genetic markers and hence can be easily adapted to other species with complex genomes. Our findings uncover several fundamental features of mammalian recombination hotspots and underline the power of the new recombination map for future studies of genetic recombination, genome stability and evolution.
ISSN: 0028-0836

hal-00877681
https://hal.archives-ouvertes.fr/hal-00877681
DOI : 10.1038/nature09869
PUBMED : 21460839

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Lithospheric-scale faults related to oblique subduction are responsible for some of the most hazardous earthquakes reported worldwide. The mega-thrust in the Sunda sector of the Sumatran oblique subduction has been intensively studied, especially after the infamous 2004 Mw 9.1 earthquake, but its onshore kinematic complement within the Sumatran subduction, the transform Sumatran Fault System, has received considerably less attention. In this paper, we apply a combination of analysis of Digital Elevation Models (ASTER GDEM) and field evidence to resolve the kinematics of the leading edge of deformation of the northern sector of the Sumatran Fault System. To this end, we mapped the northernmost tip of Sumatra, including the islands to the northwest, between 4.5°N and 6°N. Here, major topographic highs are related to different faults. Using field evidence and our GDEM structural mapping, we can show that in the area where the fault bifurcates into two fault strands, two independent kinematic regimes evolve, both consistent with the large-scale framework of the Sumatran Fault System. Whereas the eastern branch is a classic Riedel system, the western branch features a fold-and-thrust belt. The latter contractional feature accommodated significant amounts (c. 20%) of shortening of the system in the study area. Our field observations of the tip of the NSFS match a strain pattern with a western contractional domain (Pulau Weh thrust splay) and an eastern extensional domain (Pulau Aceh Riedel system), which are together characteristic of the tip of a propagating strike-slip fault, from a mechanical viewpoint. For the first time, we describe the strain partitioning resulting from the propagation of the NSFS in Sumatra mainland. Our study helps understanding complex kinematics of an evolving strike-slip system, and stresses the importance of field studies in addition to remote sensing and geophysical studies.
ISSN: 0040-1951

hal-01467196
https://hal.archives-ouvertes.fr/hal-01467196
DOI : 10.1016/j.tecto.2016.04.050

Éditeur(s) : HAL CCSD
Résumé : International audience
BACKGROUND: The aryl hydrocarbon receptor (AhR) has gradually emerged as a regulator of inflammation in the lung and other tissues. AhR may interact with the p65-subunit of the nuclear factor (NF)-κB transcription factors, but reported outcomes of AhR/NF-κB-interactions are conflicting. Some studies suggest that AhR possess pro-inflammatory activities while others suggest that AhR may be anti-inflammatory. The present study explored the impact of AhR and its binding partner AhR nuclear translocator (Arnt) on p65-activation and two differentially regulated chemokines, CXCL8 (IL-8) and CCL5 (RANTES), in human bronchial epithelial cells (BEAS-2B). RESULTS: Cells were exposed to CXCL8- and CCL5-inducing chemicals, 1-nitropyrene (1-NP) and 1-aminopyrene (1-AP) respectively, or the synthetic double-stranded RNA analogue, polyinosinic-polycytidylic acid (Poly I:C) which induced both chemokines. Only CXCL8, and not CCL5, appeared to be p65-dependent. Yet, constitutively active unligated AhR suppressed both CXCL8 and CCL5, as shown by siRNA knock-down and the AhR antagonist α-naphthoflavone. Moreover, AhR suppressed activation of p65 by TNF-α and Poly I:C as assessed by luciferase-assay and p65-phosphorylation at serine 536, without affecting basal p65-activity. In contrast, Arnt suppressed only CXCL8, but did not prevent the p65-activation directly. However, Arnt suppressed expression of the NF-κB-subunit RelB which is under transcriptional regulation by p65. Furthermore, AhR-ligands alone at high concentrations induced a moderate CXCL8-response, without affecting CCL5, but suppressed both CXCL8 and CCL5-responses by Poly I:C. CONCLUSION: AhR and Arnt may differentially and independently regulate chemokine-responses induced by both inhaled pollutants and pulmonary infections. Constitutively active, unligated AhR suppressed the activation of p65, while Arnt may possibly interfere with the action of activated p65. Moreover, ligand-activated AhR suppressed CXCL8 and CCL5 responses by other agents, but AhR ligands alone induced CXCL8 responses when given at sufficiently high concentrations, thus underscoring the duality of AhR in regulation of inflammation. We propose that AhR-signaling may be a weak activator of p65-signaling that suppresses p65-activity induced by strong activators of NF-κB, but that its anti-inflammatory properties also are due to interference with additional pathways.
Cell communication and signaling: CCS

hal-01063555
https://hal.archives-ouvertes.fr/hal-01063555
DOI : 10.1186/s12964-014-0048-8

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Paleozoic-early Mesozoic geology of Tibet was controlled by the rift-drift, seafloor spreading and subduction zone tectonics of a Paleo-Tethyan realm, which evolved between the West Cathaysides (WC) and the East Cimmerides (EC). Different suture zones with ophiolites and ophiolitic mélanges, high-pressure metamorphic belts, magmatic arcs and accretionary prism complexes separating different terranes mark multiple subduction-accretion systems within this Paleo-Tethyan domain, reminiscent of the modern Western Pacific Ocean. Discrete basins separated by different continental blocks and magmatic arcs constituted a complex paleogeography of Paleo-Tethys, and these oceanic strands were closed as a result of subduction with different polarities during the late Paleozoic-Triassic. The Longmu Tso Shuanghu-Changning Menglian Suture zone (LS-CMS) in Tibet represents the main tectonic boundary between the WC and EC that developed in the Devonian. The East Kunlun-A’nyemaqen oceanic slab was subducted northward beneath the East Kunlun terrane in northern Tibet, whereas the Sumdo oceanic slab was subducted northward beneath the South Qiangtang-North Lhasa terrane in southern Tibet. The Longmu Tso Shuanghu-Changning Menglian and Jinshajiang-Ailaoshan-Song Ma ophiolites were developed and emplaced in subduction-accretion systems with opposite polarities (westward and eastward) beneath the North Qiangtang-Qamdo-Simao-Indochina terrane in central Tibet. The Jinshajiang-Ailaoshan-Song Ma oceanic slab was subducted westward beneath the North Qiangtang-Simao-Indochina terrane along the Jinshajiang and Ailaoshan-Song Ma trenches in a trench-ridge-trench triple junction plate configuration. The Emeishan mantle plume produced a large Permian basaltic terrane, developed on the western passive margin of the South China block. The final closure of the Paleo-Tethyan oceanic branches resulted in continental collisions and development of a vast Indosinian orogenic collage in the latest Triassic-Jurassic.
ISSN: 0743-9547

insu-01119604
https://hal-insu.archives-ouvertes.fr/insu-01119604
https://hal-insu.archives-ouvertes.fr/insu-01119604/document
https://hal-insu.archives-ouvertes.fr/insu-01119604/file/Xu-JAES-2015.pdf
DOI : 10.1016/j.jseaes.2015.01.021

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Environmental factors such as pesticides can cause phenotypic changes in various organisms, including mammals. We studied the effects of the widely used herbicide atrazine (ATZ) on meiosis, a key step of gametogenesis, in male mice. METHODS: Gene expression pattern was analysed by Gene-Chip array. Genome-wide mapping of H3K4me3 marks distribution was done by ChIP-sequencing of testis tissue using Illumina technologies. RT-qPCR was used to validate differentially expressed genes or differential peaks. RESULTS: We demonstrate that exposure to ATZ reduces testosterone levels and the number of spermatozoa in the epididymis and delays meiosis. Using Gene-Chip and ChIP-Seq analysis of H3K4me3 marks, we found that a broad range of cellular functions, including GTPase activity, mitochondrial function and steroid-hormone metabolism, are affected by ATZ. Furthermore, treated mice display enriched histone H3K4me3 marks in regions of strong recombination (double-strand break sites), within very large genes and reduced marks in the pseudoautosomal region of X chromosome. CONCLUSIONS: Our data demonstrate that atrazine exposure interferes with normal meiosis, which affects spermatozoa production
ISSN: 1471-2164

hal-01225493
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01225493
DOI : 10.1186/s12864-015-2095-y
PUBMED : 26518232

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Genetics and molecular biology have been instrumental for a better understanding of heritable defects causing human infertility over the past decades. More recently, the field of reproductive biology has harnessed genome biological approaches to gain insight into molecular processes underlying normal and pathological gametogenesis and gamete function. We are currently witnessing yet another quantum leap in our ability to monitor the flow of information from the genome via the transcriptome to the proteome: tiling arrays that cover both strands of a given target genome and RNA-Seq, a method based on ultra-high throughput DNA sequencing, enable us to study noncoding and protein-coding transcripts with unprecedented precision and depth at a reasonable cost. These technologies have spawned a thriving discipline within the bioinformatics field that employs information technology for managing and interpreting biological high-throughput data. This review outlines database projects and online analysis tools useful for life scientists in general and discusses in detail selected projects that have specifically been developed for researchers and clinicians in the field of reproductive biology. This article is part of a Special Issue entitled: Molecular Genetics of Human Reproductive Failure.
ISSN: 0006-3002

hal-00877444
https://hal.archives-ouvertes.fr/hal-00877444
DOI : 10.1016/j.bbadis.2012.05.018
PUBMED : 22687534