Éditeur(s) : HAL CCSD
Résumé : International audience
o abstract
Journées Nationales Génie Côtier – Génie Civil, Dunkerque, 2-4 Juillet 2014

hal-01426583
https://hal.archives-ouvertes.fr/hal-01426583

Éditeur(s) : HAL CCSD
Résumé : International audience
This work aims to better understand the physical processes governing the wave propagation in a vertically shearedcurrent and the resulting nearshore circulation patterns. It is based on a high resolution hydro-morphodynamic fieldcampaign, ROUSTY2014, collecting a comprehensive hydro-morphodynamical dataset during a full winter season.The overall analysis highlights three main circulation patterns, largely controled by the bathymetric features and byco-working or competing wind and waves forcings. Regarding the vertical structuration of the circulation, most of fieldobservations shown seaward directed circulation with onshore component close to the air/sea surface whose intensityvaries according to incoming wave conditions. Forth, vertical shear increase when breaking happen close to sensorlocation and orientation of mean circulation depend on wind direction.
35th International Conference on Coastal Engineering
Antalya, Turkey

hal-01426582
https://hal.archives-ouvertes.fr/hal-01426582

Éditeur(s) : HAL CCSD
Résumé : International audience
Background Orodental diseases include several clinically and genetically heterogeneous disorders that can present in isolation or as part of a genetic syndrome. Due to the vast number of genes implicated in these disorders, establishing a molecular diagnosis can be challenging. We aimed to develop a targeted next-generation sequencing (NGS) assay to diagnose mutations and potentially identify novel genes mutated in this group of disorders. Methods We designed an NGS gene panel that targets 585 known and candidate genes in orodental disease. We screened a cohort of 101 unrelated patients without a molecular diagnosis referred to the Reference Centre for Oro-Dental Manifestations of Rare Diseases, Strasbourg, France, for a variety of orodental disorders including isolated and syndromic amelogenesis imperfecta (AI), isolated and syndromic selective tooth agenesis (STHAG), isolated and syndromic dentinogenesis imperfecta, isolated dentin dysplasia, otodental dysplasia and primary failure of tooth eruption. Results We discovered 21 novel pathogenic variants and identified the causative mutation in 39 unrelated patients in known genes (overall diagnostic rate: 39%). Among the largest subcohorts of patients with isolated AI (50 unrelated patients) and isolated STHAG (21 unrelated patients), we had a definitive diagnosis in 14 (27%) and 15 cases (71%), respectively. Surprisingly, COL17A1 mutations accounted for the majority of autosomal-dominant AI cases. Conclusions We have developed a novel targeted NGS assay for the efficient molecular diagnosis of a wide variety of orodental diseases. Furthermore, our panel will contribute to better understanding the contribution of these genes to orodental disease. Trial registration numbers NCT01746121 and NCT02397824
Journal of Medical Genetics

hal-01272929
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01272929
DOI : 10.1136/jmedgenet-2015-103302
PUBMED : 26502894

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Iran is an active continental domain accommodating the convergence between the Arabia and Eurasia plates. In northwestern Iran, deformation between the Central Iranian block and the Caucasus domain is mainly accommodated by right lateral strike-slip on the Tabriz fault. Cities and villages, including the city of Tabriz, have been destroyed by several strong historical earthquakes (M similar to 7). In this study, we compare the slip-rates estimated from geodetic measurements (radar interferometry and UPS) with those determined by dating a geomorphological offset of an alluvial fan along the Tabriz fault. The GPS measurements along two profiles normal to the Tabriz fault suggest a slip-rate of 7.3 +/- 1.3 mm yr(-1). The persistent scatterer radar interferometry analysis of Envisat satellite archives from 2003 to 2010 shows a velocity gradient (6 +/- 3 mm yr(-1)) across the Tabriz fault in agreement with GPS results. Moreover, it reveals that most of the area located south of the Tabriz fault is affected by subsidence, and that some sections of the fault probably act as barriers to fluid migration which may have an impact on its mechanical behaviour. West of Tabriz morphotectonic investigations on an alluvial fan surface show a right-lateral cumulative offset of 320 +/- 40 m Luminescence analyses of the coarse matrix alluvial fan deposits provide an age of 46 +/- 3 ka. This yields a slip-rate comprised between 6.5 and 7.3 mm yr(-1) along this segment. These results suggest that the Late Quaternary slip-rate is in agreement with the present-day slip-rate estimated by geodetic measurements, showing no slip-rate changes during the past 45 000 yr. Short-term variations within the 45 000 yr related to temporal earthquake clustering over few seismic cycles cannot be ruled out, but if they exist, they do not affect the geodetic and the geomorphological estimates. This study is in agreement with previous ones suggesting that long-term slip-rates (i.e. averaged over several tens of seismic cycles) are consistent with geodetic estimated slip-rates (i.e. extrapolated from few years of interseismic observations), and suggests that perturbations of fault slip-rates are related to variations over few seismic cycles.
ISSN: 0956-540X

hal-00844695
https://hal.archives-ouvertes.fr/hal-00844695
https://hal.archives-ouvertes.fr/hal-00844695v2/document
https://hal.archives-ouvertes.fr/hal-00844695/file/rizza2013.pdf
DOI : 10.1093/gji/ggt041

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BackgroundTo date, all studies conducted on breast cancer diagnosis have focused on the expression of the full-length 66-kDa estrogen receptor alpha (ERα66). However, much less attention has been paid to a shorter 46-kDa isoform (ERα46), devoid of the N-terminal region containing the transactivation function AF-1. Here, we investigated the expression levels of ERα46 in breast tumors in relation to tumor grade and size, and examined the mechanism of its generation and its specificities of coregulatory binding and its functional activities.MethodsUsing approaches combining immunohistochemistry, Western blotting, and proteomics, antibodies allowing ERα46 detection were identified and the expression levels of ERα46 were quantified in 116 ERα-positive human breast tumors. ERα46 expression upon cellular stress was studied, and coregulator bindings, transcriptional, and proliferative response were determined to both ERα isoforms.ResultsERα46 was expressed in over 70% of breast tumors at variable levels which sometimes were more abundant than ERα66, especially in differentiated, lower-grade, and smaller-sized tumors. We also found that ERα46 can be generated via internal ribosome entry site-mediated translation in the context of endoplasmic reticulum stress. The binding affinities of both unliganded and fully-activated receptors towards co-regulator peptides revealed that the respective potencies of ERα46 and ERα66 differ significantly, contributing to the differential transcriptional activity of target genes to 17β estradiol (E2). Finally, increasing amounts of ERα46 decrease the proliferation rate of MCF7 tumor cells in response to E2.ConclusionsWe found that, besides the full-length ERα66, the overlooked ERα46 isoform is also expressed in a majority of breast tumors. This finding highlights the importance of the choice of antibodies used for the diagnosis of breast cancer, which are able or not to detect the ERα46 isoform. In addition, since the function of both ERα isoforms differs, this work underlines the need to develop new technologies in order to discriminate ERα66 and ERα46 expression in breast cancer diagnosis which could have potential clinical relevance.
ISSN: 1465-5411

inserm-01412032
http://www.hal.inserm.fr/inserm-01412032
http://www.hal.inserm.fr/inserm-01412032/document
http://www.hal.inserm.fr/inserm-01412032/file/13058_2016_Article_780.pdf
DOI : 10.1186/s13058-016-0780-7

Éditeur(s) : HAL CCSD Blackwell Publishing
Résumé : International audience
Differentiating between forced regressive deposits from deglacial periods inhigh latitude domains and forced regressive deposits from the onset of glacialperiods in low latitude domains is fundamental for the accurate interpretationof glacial cycles within the geological record and then for thereconstruction of palaeogeography and palaeo-climate. A forced regressivedeglacial sequence is documented from the Lake Saint-Jean basin (Quebec,Canada). In this area, the Late Pleistocene to Holocene sediments haverecorded the Laurentide ice sheet retreat accompanied by the invasion ofmarine waters (Laflamme Gulf) from ca 129 cal kyr BP. Subsequently, fluviodeltaicand coastal prograding wedges were deposited; they followed thebase-level fall due to glacio-isostatic rebound. This succession, representinga transition from glacial to post-glacial periods within a previously glaciatedarea, was investigated through recent mapping, preserved landforms, faciesanalysis, and new optical stimulated luminescence and radiocarbon dates.Three basin-scale geological sections share a common lower part made ofisolated ice-contact fan deposits overlying bedrock. Throughout the entirebasin, ice-contact fans are capped by glacimarine muds. Above, fluvial andcoastal prograding systems were deposited and evolved through four steps:(i) deltaic systems progressively increased in width; (ii) coastal influence onsedimentation increased; (iii) hydrographic drainage systems became moreorganised; and (iv) deltas graded from steep (Gilbert delta) to low-angle foresets(mouth-bar delta). Deposited during the base-level fall from glacioisostaticrebound, the complete succession has been designated as a singlefalling stage system tract referred to as a deglacial falling stage system tract.It is representative of a deglaciation sequence in areas previously covered byice during glacial periods (i.e. medium to high latitude domains). Diagnosticcriteria are provided to identify such a deglacial falling stage system tract inthe geological record, which may aid identification of previously unknownglacial cycles.
ISSN: 1469-3496

insu-01184731
https://hal-insu.archives-ouvertes.fr/insu-01184731
DOI : 10.1111/sed.12196

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This manuscript reports on a novel field experiment carried out on a microtidal beach in Camargue, France. For the first time in the field, a comprehensive description of the groundwater dynamics under sandy beach swash zone is presented. A cross-shore network of 15 buried pressure sensors is combined with terrestrial LiDAR measurements to study the swash-groundwater dynamics. The presented data focus on the decay of a moderate storm which allows to monitor the evolution of the groundwater pressure field in response to the retreat of the swash zone. Both horizontal and vertical head gradients are measured within the porous sand soil to estimate the groundwater flow field using Darcy’s law. Time-averaged analysis demonstrates the presence of a rather consistent groundwater circulation pattern under the swash zone, shifting offshore with the swash zone. The main tendency is an offshore directed flow, with infiltration/exfiltration in the upper/lower parts of the swash zone. Time-resolved analysis highlights the typical groundwater response to swash events which consists mainly of an overall infiltration flow during the bed inundation by the swash tongue, a seaward flow during the swash retreat and, for long backwash events, a localized exfiltration flow under the next incoming uprush.
ISSN: 0309-1708

hal-01412450
https://hal.archives-ouvertes.fr/hal-01412450
DOI : 10.1016/j.advwatres.2016.09.009

Éditeur(s) : HAL CCSD
Résumé : International audience
BIOEM
Hangzhou, China

hal-01627606
https://hal.archives-ouvertes.fr/hal-01627606

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Mechanical forces influence the growth and shape of virtually all tissues and organs. Recent studies show that increased cell contractibility, growth and differentiation might be normalized by modulating cell tensions. Particularly, the role of these tensions applied by the extracellular matrix during liver fibrosis could influence the hepatocarcinogenesis process. The objective of this study is to determine if 3D stiffness could influence growth and phenotype of normal and transformed hepatocytes and to integrate extracellular matrix (ECM) stiffness to tensional homeostasis. We have developed an appropriate 3D culture model: hepatic cells within three-dimensional collagen matrices with varying rigidity. Our results demonstrate that the rigidity influenced the cell phenotype and induced spheroid clusters development whereas in soft matrices, Huh7 transformed cells were less proliferative, well-spread and flattened. We confirmed that ERK1 played a predominant role over ERK2 in cisplatin-induced death, whereas ERK2 mainly controlled proliferation. As compared to 2D culture, 3D cultures are associated with epithelial markers expression. Interestingly, proliferation of normal hepatocytes was also induced in rigid gels. Furthermore, biotransformation activities are increased in 3D gels, where CYP1A2 enzyme can be highly induced/activated in primary culture of human hepatocytes embedded in the matrix. In conclusion, we demonstrated that increasing 3D rigidity could promote proliferation and spheroid developments of liver cells demonstrating that 3D collagen gels are an attractive tool for studying rigidity-dependent homeostasis of the liver cells embedded in the matrix and should be privileged for both chronic toxicological and pharmacological drug screening.
ISSN: 0730-2312

hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/file/Increasing%203D%20Matrix%20Rigidity_accepted.pdf
DOI : 10.1002/jcb.25356

Éditeur(s) : HAL CCSD
Résumé : International audience
This work presents a synthesis of the present-day knowledge on both emerged and submerged carbonate platforms of the Guadeloupe archipelago. Onshore and offshore data acquired during the KaShallow project are presented. Since the early Zanclean, some isolated platforms developed onto a Jurassic to Tortonian basement which displayed reliefs inherited from a major extensional tectonic episode dated from −8 to −5 Ma. Marie-Galante, Grande Terre and the southern shelf of Grande-Terre show strong similarities with Zanclean to Gelasian red-algal facies upward changing into early Calabrian coral facies. A major erosional unconformity (SB2) was recognized and allows to distinguish two early Calabrian formations (Calcaires à Agaricia Formation and Calcaires à Acropora Formation). The final emersion of these platforms is late Calabrian. The La Désirade platform displays Zanclean to early Piacenzian red-algal facies upward changing into coral facies. This platform emerged as soon as in the late Piacenzian. The island subsided again in relation with later extensional tectonic episode and early Calabrian reef platform then unconformably deposited. The Colombie bank deposits comprise Pliocene basinal deposits overlain by late Calabrian coral reefs. The Flandre bank might have emerged synchronously with La Désirade but was drowned only in the late Pleistocene-Holocene interval. Four extensional tectonic episodes have been identified in the late Miocene-Recent interval. Their effects depend on the location of the platforms within the fore-arc.
Bulletin Société Géologique de France

insu-00816463
https://hal-insu.archives-ouvertes.fr/insu-00816463
DOI : 10.2113/gssgfbull.184.1-2.99

Éditeur(s) : HAL CCSD
Résumé : International audience
Two opposing visions of the Aegean backarc tectonics implicitly contain contrasting images of the rheological behaviour of the lihosphere, supported by different sets of observations. The propagation of the NAF and extension in the Corinth Rift suggest a strongly localising rheology whereas the formation of MCC in the Cyclades and the Rhodope suggests instead a more viscous behaviour. This paradox was the seminal question addressed by the ANR-EGEO programme (2007-2010) that leads to the following conclusions: (1) The exhumation of the Cycladic MCC's is accommodated by the N-dipping North Cycladic Detachment System (NCDS) that partly reworks the Vardar suture and at the base by a series of thrusts, including the basal contact of the Cycladic Blueschists (CBS) over the Cycladic Basement (CB). The activity of LANF is due to the reactivation of pre-existing discontinuities such as thrusts or earlier detachments and much less to the interaction with granitic plutons. (2) Exhumation in the Cyclades has proceeded in two stages: (a) Eocene syn-orogenic exhumation within the subduction lower/upperplate interface while the post-orogenic Rhodope MCC formed further north, (b) Oligo-Miocene post-orogenic extension in an MCC mode coeval with syn-orogenic exhumation in Crete and the Peloponnese. (3) Both stages were associated with slab retreat, as early as the Eocene with an acceleration at 30-35 Ma. (4) The localisation of the presently active steeply-dipping normal faults on the southern margin of the Corinth Rift may have been preceded by a partial reworking of thrusts and syn-orogenic detachments by shallow-dipping decollements. (5) The localisation of the NAF in the Marmara Sea region and the Northern Aegean Sea is now accurately dated between 5.3 and 5 Ma Ma using the erosion and deposition surfaces that mark the Messinian salinity Crisis and nannofossils ages. (6) The formation of MCCs in the Cyclades resulted in the draining of the low viscosity lower crust from the northern Aegean Sea and led to an easier coupling between upper mantle and upper crust, easing the localisation and propagation of the NAF. (7) A comparison between crustal and mantle finite strain suggests that mantle flow due to slab retreat controls the stretching of the crust. Our model involves progressive slab retreat and mantle flow below the overriding plate inducing the observed succession of tectonic episodes with a progressive localisation of extension due to several slab tearing events. There is no paradox in the tectonic history and rheological behaviour of the Aegean lithosphere but an evolution of the kinematic boundary conditions due to slab deformation at depth.
American Geophysical Union
San Francisco, United States

insu-00860518
https://hal-insu.archives-ouvertes.fr/insu-00860518
BIBCODE : 2010AGUFM.T13G..04J