Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00018

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Les grands domaines naturels, rattachés aux diverses stations de villégiature, étaient dépositaires des meilleurs lacs à truites, d’immenses territoires de sauvagine, de cerf de Virginie et d’un grand nombre de rivières à saumon. Si le littoral et la montagne ont agi comme attracteurs dans le cas de la villégiature, il en a été autrement pour les clubs de chasse et de pêche, qui sont apparus au milieu du XIXe siècle et se sont multipliés à compter de 1885. En l’occurrence, la mécanique à l’œuvre semble avoir posé la « grande nature » comme visée territoriale et le « coureur des bois » comme figure emblématique du héros romantique. L’argument développé dans cet article va s’employer à montrer que le tourisme et la villégiature ont constitué au Québec des procédures de valorisation positionnelle de nature géoanthropologique. Le « clubage » des Laurentides, au sens large du terme, aura constitué en ce sens le présupposé de leur colonisation intérieure. Qu’il s’agisse du lac Saint-Jean, de la Mauricie ou des Laurentides en général, on retrouve l’efficacité du parcours structural de l’établissement humain développé en géographie structurale : l’occupation utilitaire d’un territoire disponible présuppose non pas d’abord une transformation de la nature, mais une valorisation des positions abstraites par le tourisme et la villégiature. 
The large natural areas, linked to the various resorts, were custodians of the best trout lakes, huge areas of waterfowl, deer of Virginia and a large number of salmon rivers. If the coast and the mountains acted as attractors in the case of the holiday, it was different for the club hunting and fishing, which have emerged in the mid-nineteenth century and have increased from 1885. In this case, the mechanics at work seems to have raised the "great outdoors" as a territorial and "rider of the woods" as emblematic figure of the romantic hero. In this case, the mechanics at work seems to have raised the "great outdoors" as a territorial and "rider of the woods" as emblematic figure of the romantic hero. The argument developed in this article will seek to show that tourism and vacationing were in Quebec recovery procedures such as positional geo-anthropological. The “clubage” Laurentian, in the broadest sense, will be formed in this sense the assumption of internal colonization. Whether Lake Saint-Jean, Mauricie and Laurentides in general, we find the effectiveness of the structural pathways of human settlement geography developed structural: the occupation of territory utility presupposes not available not primarily a transformation of nature but a recovery of vague ... by the tourism and the holiday resort.
Québec

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.3609
http://journals.openedition.org/etudescaribeennes/3609

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00030

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00008

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00038

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Prior to 2011, there were only two international airports in Jamaica, Norman Manley International Airport (NMIA) located in Kingston and Sangster International Airport (SIA) located in Montego Bay. NMIA was originally named Palisadoes Airport and was renamed for Jamaica’s second Chief Minister and national hero Norman Washington Manley. The SIA airport was originally named Montego Bay Airport. It was renamed for Jamaica’s second prime minister Sir Donald Sangster. This image shows an airplane wing and aerial view of Jamaica. Slide labeled approach to Jamaica.
Jamaica -- Caribbean region

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 59:6
http://ufdc.ufl.edu/AA00030848/00001

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00048

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00020

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00053

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00059

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00041

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00060

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
In 1953, the co-educational school Moore Town Primary and Junior High School in Portland, Jamaica was established as an all age school. The Bump Grave monument of Nanny of the Maroons is erected across the road at the front of the school. Nanny sometimes known as, Granny Nanny, is Jamaica’s only female National Hero. In this image, some school children dressed in school uniforms are standing outside the one-room school building. Slide labeled Jam. School in Moore Town.
Jamaica -- Caribbean region -- Moore Town, Portland

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
http://ufdc.ufl.edu/AA00031038/00001

Éditeur(s) : Scientific Technical Research Council Turkey-tubitak
Résumé : In 2002, the Bosphorus outlet was mapped using an EM 300 multibeam echo-sounder together with a Chirp sonar system. This survey, carried out on board the Ifremer RV 'Le Suroit' in the frame of the BlaSON project, completes the data previously acquired directly at the mouth of the Bosphorus by Di Iorio et al. (1999) in the frame of a NATO SACLANT Undersea Research project using jointly the NATO RV Alliance, and the Turkish Navy Survey ship 'Cubuklu'. This acoustic imaging has identified a canyon system at the slope and a shallow marine fan, which contains shelf incisions extending the Istanbul Strait incision. Multibeam bathymetry, ultra-high resolution seismic profiling and coring correlations on this subaqueous fan area allowed reconstruction of morphology and patterns of sediment distribution indicative of high energy sediment transport processes. The discovery of a shallow water/shelf type fan directly off shore from the Bosphorus and connected to its outlet is consistent with the theories of sudden discharges of large volumes of water. Age dating obtained at the bottom of this subaqueous shelf fan yielded an age of 6700 yr C-14 BP (uncorrected age) for the first marine mollusc encountered at the base. This is in accordance with a last and abrupt reconnection of the Marmara Sea to the Black Sea. A detailed morphological map of the shelf and slope along with seismic profile interpretation and core correlation is presented here. A synthesis is proposed to explain the formation of this subaqueous fan and its relationship with the last connection between Black and Marmara seas aft er the Last Glacial Maximum. This interpretation can be summarized as follows: stage A corresponds to the first erosion surface seen on the shelf related to the Last Glacial Maximum low stand; stage B is the ravine surface onlapping to ca. -30, -40 m; stage C is a second erosional surface related to a sea level fall and eroding most of underlying Unit 1B; and stage D corresponds to the onset of the fan deposit during a period of high water run-off from the Black Sea entering from the Bosphorus. Avulsion branches show that this fan has been active for a long time.
Turkish Journal Of Earth Sciences (1300-0985) (Scientific Technical Research Council Turkey-tubitak), 2011 , Vol. 20 , N. 3 , P. 287-305

Droits : 2011 TÜBİTAK
http://archimer.ifremer.fr/doc/00036/14676/13677.pdf
http://archimer.ifremer.fr/doc/00036/14676/

Éditeur(s) : Neidine Editeurs
Résumé : This article has for objective to put forward the ethical values of the sea and the ocean. After a reminder(abs of the marine origin of life on earth and what the human always carries in him tracks of this origin, we underline several characteristics: the regulation of the climate by the ocean, the specificities of part of the animal kingdom which unlike other did not conquer the terrestrial domain, the fact that marine biological material was at the origin of numerous Nobel prizes, finally which the sea holds number of biological, mineral, energy resources etc. which will be the resources of tomorrow. The following part is dedicated to the human values which the sea inculcates to those who frequent her. We offer a list of some current expressions of the French language the origin of which is marine or maritime. We discuss then about prosperous nations, sometimes small ones, which knew how to put forward the values of the sea and benefit from it for their influence. We talk about the symbols pulled by the sea, in particular the shells and their value, which various civilizations and peoples have known to adopt. We end on the New Caledonian case and on the opportunities and the assets which can be, considering its marine and maritime values as an island Pacific country.
Cet article a pour objectif de mettre en avant les valeurs éthiques de la mer et de l’océan. Après un rappel de l’origine marine de la vie sur terre et du fait que l’homme porte toujours en lui des traces de cette origine, on souligne plusieurs caractéristiques : la régulation du climat par l’océan, les spécificités de certains embranchements du règne animal qui à la différence d’autres n’ont pas conquis le domaine terrestre, le fait que matériel biologique marin a été à l’origine de nombreux prix Nobel, enfin que la mer recèle nombre de ressources biologiques, minérales, énergétiques etc… qui seront les ressources de demain. La partie suivante est consacrée aux valeurs humaines que la mer inculque à ceux qui la fréquentent. On offre une liste d’expressions courantes de la langue française dont l’origine est marine ou maritime. On discute ensuite des nations florissantes, parfois petites, qui ont su mettre en avant les valeurs de la mer et en tirer profit pour leur rayonnement. On disserte sur les symboles tirés de la mer et notamment des coquillages et de leur valeur que différentes civilisations et peuples ont su adopter. On conclue sur le cas calédonien et sur les opportunités et atouts qui peuvent être les siens de considérer ses valeurs marines et maritimes en tant que pays insulaire du Pacifique.
Tai Kona (2269-7535) (Neidine Editeurs), 2014-07 , N. 9 , P. 28-43

Droits : Tai Kona
http://archimer.ifremer.fr/doc/00203/31393/29782.pdf
http://archimer.ifremer.fr/doc/00203/31393/

Éditeur(s) : Aida García Alonso Aida García Alonso ( Habana, Cuba )
Résumé : (Acquisition) From private collection.
(Biographical) Aida García Alonso was Cuban. She attended Mexico's National School of Anthropology and History (ENAH) where she got a degree in cultural anthropology. After the Cuban Revolution, García Alonso returned to Cuba. There she started to do ethnographic research in Las Yaguas, a series of slums in Havana. Her work coincided with the Cuban government's project of relocating Las Yaguas' dwellers to new houses built by the new regime. The result of her fieldwork was the book Manuela la Mexicana published by Casa de las Americas in 1968. In the 1970s, García Alonso was preparing a second edition. For this newer edition she prepared photographs and statistics on the people who used to live in Las Yaguas and was relocated. Fidel Castro's government censored the work, and García Alonso went into exile to Mexico in 1980, where she died in 2009. This digital collection includes the photographs, captions, and statistics that were to be included in the second edition of Manuela la Mexicana.
Havana, Cuba

Droits : Applicable rights reserved.
http://ufdc.ufl.edu/AA00019386/00032

Éditeur(s) : HAL CCSD
Résumé : International audience
Many countries are legally obliged to embrace ecosystem-based approaches to fisheries management. Reductions in bycatch and physical habitat damage are now commonplace, but mitigating more sophisticated impacts associated with the ecological functions of target fisheries species are in their infancy. Here we model the impacts of a parrotfish fishery on the future state and resilience of Caribbean coral reefs, enabling us to view the tradeoff between harvest and ecosystem health. We find that the implementation of a simple and enforceable size restriction of >30 cm provides a win:win outcome in the short term, delivering both ecological and fisheries benefits and leading to increased yield and greater coral recovery rate for a given harvest rate. However, maintaining resilient coral reefs even until 2030 requires the addition of harvest limitations (<10% of virgin fishable biomass) to cope with a changing climate and induced coral disturbances, even in reefs that are relatively healthy today. Managing parrotfish is not a panacea for protecting coral reefs but can play a role in sustaining the health of reefs and high-quality habitat for reef fisheries.
http://hal.univ-reunion.fr/hal-01369752

hal-01369752
http://hal.univ-reunion.fr/hal-01369752
DOI : 10.1073/pnas.1601529113

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : International audience
Abstract The continuous record of large surface-rupturing earthquakes along the Dead Sea fault brings unprecedented insights for paleoseismic and archaeoseismic research. In most recent studies, paleoseismic trenching documents the late Holocene faulting activity, while tectonic geomorphology addresses the long-term behavior (>10 ka), with a tendency to smooth the effect of individual earthquake rupture events (Mw >7). Here, we combine historical, archaeological, and paleoseismic investigations to build a consolidated catalog of destructive surface-rupturing earthquakes for the last 14 ka along the left-lateral Jordan Valley fault segment. The 120- km-long fault segment limited to the north and the south by major pull-apart basins (the Hula and the Dead Sea, respectively) is mapped in detail and shows five subsegments with narrow stepovers (width < 3 km). We conducted quantitative geomorphology along the fault, measured more than 20 offset drainages, excavated four trenches at two sites, and investigated archaeological sites with seismic damage in the Jordan Valley. Our results in paleoseismic trenching with 28 radiocarbon datings and the archaeoseismology at Tell Saydiyeh, supplemented with a rich historical seismic record, document 12 surface-rupturing events along the fault segment with a mean interval of ∼1160 yr and an average 5 mm=yr slip rate for the last 25 ka. The most complete part of the catalog indicates recurrence intervals that vary from 280 yr to 1500 yr, with a median value of 790 yr, and suggests an episodic behavior for the Jordan Valley fault. Our study allows a better constraint of the seismic cycle and related short-term variations (late Holocene) versus long-term behavior (Holocene and late Pleistocene) of a major continental transform fault.
ISSN: 0037-1106

hal-00747401
https://hal.archives-ouvertes.fr/hal-00747401
DOI : 10.1785/0120100097

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
We show here that the Amalaoulaou complex, in the Pan-African belt of West Africa (Gourma, Mali), corresponds to the lower and middle sections of a Neoproterozoic intra-oceanic arc. This complex records a 90-130-Ma-long evolution of magmatic inputs and differentiation above a subducting oceanic slab. Early c. 793 Ma-old metagabbros crystallised at lower crustal or uppermost mantle depths (25-30 km) and have geochemical characteristic of high-alumina basalts extracted from a depleted mantle source slightly enriched by slab-derived sedimentary components ((La/Sm)(N) < 1; epsilon(Nd): +5.4-6.2; Sr-87/Sr-86: 0.7027-0.7029). In response to crustal thickening, these mafic rocks were recrystallised into garnet-granulites (850-1,000A degrees C; 10-12 kbar) and subject to local dehydration-melting reactions, forming trondhjemititic leucosomes with garnet-clinopyroxene-rutile residues. Slightly after the granulitic event, the arc root was subject to strong HT shearing during partial exhumation (detachment faults/rifting or thrusting), coeval with the emplacement of spinel- and garnet-pyroxenite dykes crystallised from a high-Mg andesitic parental magma. Quartz and hornblende-gabbros (700-660 Ma) with composition typical of hydrous volcanic rocks from mature arcs ((La/Sm)(N): 0.9-1.8; epsilon(Nd): +4.6 to +5.2; Sr-87/Sr-86: 0.7028-0.7031) were subsequently emplaced at mid-arc crust levels (similar to 15 km). Trace element and isotopic data indicate that magmas tapped a depleted mantle source significantly more enriched in oceanic sedimentary components (0.2%). Exhumation occurred either in two stages (700-660 and 623 Ma) or in one stage (623 Ma) with a final exhumation of the arc root along cold P-T path (550A degrees C, 6-9 kbar; epidote-amphibolite and greenschist facies conditions) during the main Pan-African collision event (620-580 Ma). The composition of magmas forming the Cryogenian Amalaoulaou arc and the processes leading to intra-arc differentiation are strikingly comparable to those observed in the deep section of exposed Mezosoic oceanic arcs, namely the Kohistan and Talkeetna complex. This evolution of the Amalaoulaou oceanic arc and its accretion towards the West African craton belong to the life and closure of the Pharusian Ocean that eventually led to the formation of the Greater Gondwana supercontinent, a similar story having occurred on the other side of the Sahara with the Mozambique Ocean.
ISSN: 0010-7999

hal-00639447
https://hal.archives-ouvertes.fr/hal-00639447
DOI : 10.1007/s00410-011-0624-5