Éditeur(s) : HAL CCSD
Résumé : International audience
South-east Iran is the site of a rare case of young transition between subduction and collision. We synthesized recent results in geodesy, tectonics, seismology and magnetism to understand the structure and kinematics of the Zagros-Makran transition. Surface observations (tectonics, magnetism and geodesy) draw a transpressive discontinuity consisting of several faults striking obliquely to the convergent plate motion, whereas deeper observations (seismology) support a smooth transition across the fault system. No lithospheric transform fault has been created, even though the transition already behaves like a major boundary in terms of tectonic style, seismic structure, lithology and magnetism. The Zendan-Minab-Palami fault system consists of several faults that accommodate a transpressive tectonic regime. It is the surface expression of a southward propagation of the N-trending right-lateral strike-slip fault system of Jiroft-Sabzevaran. Within each system the numerous faults will coalesce into a single, lithospheric, wrench fault.
ISSN: 0375-6440

hal-00356532
https://hal.archives-ouvertes.fr/hal-00356532
https://hal.archives-ouvertes.fr/hal-00356532/document
https://hal.archives-ouvertes.fr/hal-00356532/file/Regard_MinabSynthese_revised_AvecFigs.pdf
DOI : 10.1144/SP330.4

Éditeur(s) : Oxford Univ Press
Résumé : In subduction zones, melting and dehydration of the subducted slab introduce material into the mantle wedge and modify its chemical and isotopic composition. As a consequence, island arc lavas differ significantly from mid-ocean ridge basalts and ocean island basalts. In some arcs, the composition of lavas is strongly influenced by the sedimentary material introduced with the slab; in others, magma composition is mainly affected by aqueous fluids released by the slab. The Lesser Antilles arc is known for its extreme continental-crust-like signature but for some Lesser Antilles lavas subducted sediments are barely involved and enrichment in fluid-mobile elements (Ba, U, Sr, Pb, etc.) is the dominant feature. Here we evaluate whether La/Sm is a quantitative proxy of sediment involvement in volcanic arcs, and we relate dehydration and melting processes to the temperature and pressure conditions of the slab. We use Martinique as a case study because in this island both dehydration and sediment melting fingerprints coexist. We measured major and trace elements for about 130 age-constrained samples, carefully chosen to cover all volcanic phases of Martinique (25 Ma to present). Using these results we demonstrate that: (1) weathering does not modify the La/Sm ratio; (2) fractional crystallization of amphibole and/or garnet does not increase La/Sm by more than 20%; (3) rare earth element transfer from wall-rock to magma during fractionation is not significant; (4) melting of the mantle source increases La/Sm by only about 20%. As a consequence, we show that the proportion of slab sediment incorporated in the mantle wedge controls the La/Sm ratio of the source. The observed correlations between La/Sm and Nd and Hf isotopic compositions indicate that the effect of sediment addition is the overwhelming factor: La/Sm is a good proxy for slab sediment proportion in Martinique. We observe a geographical gradient between slab dehydration and sediment melting on the island. Whereas lavas located on the western side of the island display a clear sedimentary input in their source, lavas located on the eastern side of the island, closer to the trench, are clearly influenced by dehydration of the subducted slab. In addition, the aqueous fluids clearly come from the subducted basalt and they did not interact with the overlying sediments. The influence of sediment added to the source of the magmas increases from the eastern part to the western part of the island. We relate this geographical change to the pressure and temperature conditions at the slab surface. Sediments probably cross their solidus under Martinique and hydrous melting is triggered. Finally, we show that under all volcanic arcs where the signature of sediments overwhelms the signature of fluids, the slab surface reaches P-T conditions that allow the subducted sediments to melt. Inversely, under most volcanic arcs where the signal of aqueous fluids dominates over sediment melts, the subducted slab is not hot enough for the sedimentary pile to melt.
Journal Of Petrology (0022-3530) (Oxford Univ Press), 2012-12 , Vol. 53 , N. 12 , P. 2441-2464

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00113/22392/20234.pdf
DOI:10.1093/petrology/egs055
http://archimer.ifremer.fr/doc/00113/22392/

Résumé : After a period of rapid development of the sites (an increase in both reared surface area and production), tropical shrimp aquaculture is currently being faced with critical problems due to economical and ecological constraints. In many countries with favourable conditions for shrimp aquaculture such as indonesia or Vietnam, sites are often badly selected and/or over-exploited. This conducts to sorne extent to decreases or collapses in the production due to the difficulty in predicting the maximum production capacity of the sites. Furthermore, it appears that production sustainability depends on many factors among which socio-economy and ecolo gy are of prime importance. The STD3 project titled « Assessment of tropical shrimp aquaculture impact on the environment in tropical countries using hydrobiology, ecology and remote sensing as helping tools for diagnosis )) was initiated in 1994, with the aim to study the sustainability of marine shrimp aquaculture in tropical areas, main! y Indonesia and Vietnam, through the following tasks: (i) improve site selection and study the impact of aquaculture on marine environments presenting a variable sensitivity to organic sewage, (ii) analyse the socio-economical aspects and profitability of the aquaculture industry and of common resources (iii) use remote sensing and geographical data bases for diagnosis and monitoring of site degradation (iv) reinforce the capacity of Asian scientists through training. Severa! aquaculture sites have been investigated in the Lampung region (South Sumatra, Indonesia), the Mekong delta (Vietnam) and on the West coast of New-Caledonia, providing a large range of typical ecosystems encountered in Asia and in the Pacifie, from coralline sites to coastal plains with mangrove and deltaic areas. The study concerned the spatial structure and seasonal influence of these ecosystems, the temporal variation during the course of the project (3 years) and the relation between the ecological structure and farm productions. Remote sensing provides a synoptic vision over large land expanses. Severa! scenes were processed for landuse mapping using conventional classification techniques. Concerning water quality assessment, a general relation for the Java sea was found between image and field data in terms of total suspended matter. Applying this relation to a new site in Sumatra has provided an initial approach to water type and, together with land use mapping, a preliminary assessment of the suitability of the area to shrimp aquaculture development. Socio-economic research has been focused on the identification ofneeds for collective action, including public policy, in the perspective of shrimp farming sustainability. The main concern is in the regulation of shrimp farming intensification and extensification at the scale of coastal ecosystemic entities in a common property resource management perspective. The common considered here is coastal water quality. Comparing the local development profiles and the institutional grounds for the design and implementation of collective management rules shows that variables such as land tenure system, farm owner socio-economic profile, social homogeneity or heterogeneity of the farmers are key factors to analyse the potential for sustainability. The defmition of water quality and the possible means to ensure its collective management are discussed. The main conclusion is that the economie incentives to farming development are strong but there is no significant difference among traditional, semi-intensive and intensive systems in terms of economie efficiency or wealth distribution. The description of the functioning of the different kinds of ecosystems make it possible to give an advice concerning the positioning of the activity inside the ecosystem, and furthermore to have a reference state in order to determine the impact of the activity on the coastal environnement. This impact can be summarized as an increase in the concentration of total suspended matt€?r, of particulate organic matter and of sulfate reducing bacterias. This organic ma~.er is either issued from rearing activities or from telluric origine, due to mangrove eradication when building the ponds. An evaluation of the relationship existing between productivity and characteristics of each ecosystem bas been conducted by correlating ecological indicators data (concentration of total suspended matter, particulate organic matter, chlorophyll, percentage of pheopigments in total pigments and cyanobacteria and sulfatereducing bacteria in water and sediments) with the average production of the farms. This comparison lead to the compilation of a scale of observed production integrating the range of these environmental parameters with shrimp production levels, bound to facilitate the positioning of shrimp farming in relation with the confmement leve! of each ecosystem. Lastly, ali geographie data originated from the various compartments of the study have been geo-referenced and loaded into a geographical information system. This allows to display any query made on spatial variables and their related statistical data, including the ir variations over the last few years and to reveal patterns and phenomena otherwise not obvious.
Le programme mis en oeuvre par la DRV (Paris, CREMA-L'Houmeau, DELIAO Brest, COP Tahiti, GIE/RA Nouvelle Calédonie), dans le cadre d'un projet européen STD3 «(Sciences et Technique au Service du Développement», en collaboration avec l'Université de Montpellier II, l'Ecole Nationale d'Agronomie de Rennes, l'ITC (Hollande), l'Institut Océanographique de Nha Trang (Vietnam), le BADC de Jepara et le BPPT de Jakarta (Indonésie), avait pour but la mise au point de méthodes permettant d'améliorer la sélection des sites ainsi que le suivi du développement et de l'impact de l'aquaculture des crevettes Péneides en milieu tropical pour éviter la surexploitation. Quatre axes de recherche ont été défmis : a) identifier la nature et quantifier les rejets de déchets issus de ce type d'aquaculture, b) définir les caractéristiques écologiques des écosystèmes susceptibles d'accueillir l'activité aquacole et suivre leur évolution sous l'influence des rejets, c) déterminer l'influence de la gestion des fermes et des ressources (aspects économiques) sur les performances de productivité, d) déterminer la capacité de la télédétection comme outil de diagnostic d'impact à l'échelle de la région, e) mener un programme de formation d'étudiants et jeunes chercheurs Vietnamiens et Indonésiens.

Droits : 1998 Ifremer
http://archimer.ifremer.fr/doc/00132/24357/22357.pdf
http://archimer.ifremer.fr/doc/00132/24357/

Éditeur(s) : Amer Meteorological Soc
Résumé : The Congo River has the second largest rate of flow in the world and is mainly responsible for the broad tongue of low-salinity water that is observed in the Gulf of Guinea. Despite their importance, near-equatorial river plumes have not been studied as thoroughly as midlatitude plumes and their dynamics remain unclear. Using both theory and idealized numerical experiments that reproduce the major characteristics of the region, the authors have investigated the dynamics of the Congo River plume and examine its sensitivity to different forcing mechanisms. It is found that near-equatorial plumes are more likely to be surface trapped than midlatitude plumes, and the importance of the effect in describing the strong offshore extent of the low-salinity tongue during most of the year is demonstrated. It is shown that the buoyant plume constrained by the geomorphology is subject to the pulling of nonlinear structures and wavelike equatorial dynamics. The wind is found to strengthen the intrinsic buoyancy-driven dynamics and impede the development of the coastal southward current, in coherence with observations.
Journal Of Physical Oceanography (0022-3670) (Amer Meteorological Soc), 2014-03 , Vol. 44 , N. 3 , P. 980-994

Droits : 2014 American Meteorological Society
http://archimer.ifremer.fr/doc/00187/29850/28298.pdf
DOI:10.1175/JPO-D-13-0132.1
http://archimer.ifremer.fr/doc/00187/29850/

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : International audience
During the GWADASEIS cruise (Lesser Antilles volcanic arc, February-March 2009) a very high resolution (VHR) seismic-reflection survey was performed in order to constrain Late Quaternary to Present faulting. The profiles we obtained evidence frequent "ponding" of reworked sediments in the deepest areas, similar to the deposition of Mediterranean "homogenites". These bodies are acoustically transparent (few ms t.w.t. thick) and are often deposited on the hanging walls of dominantly normal faults, at the base of scarps. Their thickness appears sufficient to compensate (i.e. bury) co-seismic scarps between successive earthquakes, resulting in a flat and horizontal sea floor through time. In a selected area (offshore Montserrat and Nevis islands), piston coring (4 to 7 m long) was dedicated to a sedimentological analysis of the most recent of these particular layers. It corresponds to non-stratified homogenous calcareous silty sand (reworked calcareous plankton and minor volcanoclastics). This layer can be up to 2 m thick, and overlies finegrained hemipelagites. The upper centimeters of the latter represent the normal RedOx water/sediment interface. 210Pb and 137Cs activities lack in the massive sands, while a normal profile of unsupported 210Pb decrease is observed in the hemipelagite below, together with a 137Cs peak correspond- ing to the Atmospheric Nuclear Experiments (1962). The RedOx level was thus capped by a recent instantaneous major sedimentary event considered as post-1970 AD; candidate seismic events to explain this sedimentary deposits are either the 16 March 1985 earthquake or the 8 October 1974 one (Mw=6.3 and Mw=7.4, respectively). This leads to consider that the syntectonic sedimentation in this area is not continuous but results from accumulation of thick homogenites deposited after the earthquakes (as observed in the following weeks after Haiti January 2010 event, McHugh et al., 2011). The existence of such deposits suggests that, in the area of study, vertical throw likely results from cumulated effects of separated earthquakes rather than from aseismic creep. Examination of VHR profiles shows that all major co-seismic offsets are recorded in the fault growth sequence and that co-seismic offsets can be precisely estimated. By using a sedimentation rate deduced from 210Pb decrease curve (0.5 mm yr−1) and taking into account minor reworking events detected in cores, we show that the Redonda system may have been responsible for five >M6 events during the last 34 000 yr. The approach presented in this work differs from fault activity analyses using displaced sets of isochronous surfaces and postulating co-seismic offsets. Combining VHR seismic imagery and coring we can decipher co-seismic vs. slow continuous displacement, and thus actually estimate the amplitude and the time distribution of major co-seismic offsets.
ISSN: 1561-8633

insu-00844581
https://hal-insu.archives-ouvertes.fr/insu-00844581
https://hal-insu.archives-ouvertes.fr/insu-00844581/document
https://hal-insu.archives-ouvertes.fr/insu-00844581/file/nhess-12-1755-2012.pdf
DOI : 10.5194/nhess-12-1755-2012

Éditeur(s) : HAL CCSD
Résumé : Cette étude se situe dans la perspective de l'analyse de l'évolution post-messinienne de la marge ouest-tyrrhénienne (programme Metyss). Les pentes accidentées des reliefs granitiques au dessus de la vallée du Cedrino sont couvertes de l'amont vers l'aval par des blocs chaotiques, des accumulations de poudingues et brèches polygéniques ou monogéniques qui sont irrégulièrement cimentées et, plus localement, par des séquences gravelo-sableuses granoclassées de crues torrentielles dessinant des structures en auge. Plus bas, d'autres séquences plutôt sablo-silteuses correspondent à des éventails de chenaux anastomosés de plaine alluviale. En transgression sur ces dépôts de piedmont ou plus ou moins directement sur un socle granitique ou encore schisteux, plusieurs faciès marins composés aussi de sables granitiques relativement bien triés sont observés en bordure d'un golfe. L'ensemble des dépôts ont livré une microfaune permettant de leur attribuer un âge Pliocène supérieur et parfois proche de la limite Pliocène-Pléistocène. Ils correspondent à des environnements bathymétriques variés (littoral côtier, pro-delta, plateforme ou haut de pente) qui se succèdent verticalement sur seulement quelques dizaines de mètres. Les successions observées ne correspondent pas à des séquences transgressives classiques: en plusieurs cas, la séquence débute par un dépôt relativement profond, puis montre des oscillations variables avec des tendances très littorales, avant l'émersion finale, ailleurs la séquence marine est réduite à un seul dépôt littoral. Ces séquences dont les termes sont variables dans l'espace impliquent une activité tectonique synsédimentaire qui est attestée par plusieurs accidents et par des éboulements de falaise. Enfin, tous ces dépôts marins et continentaux sont cachetés par un ensemble de coulées basaltiques d'âge pléistocène. Les accumulations détritiques marines évoquent des corps sableux de type " Gilbert delta ". Cette étude montre que de tels édifices n'existent pas tout au moins à l'échelle de cette zone périlittorale. En effet, la géométrie des corps sableux n'est pas orientée vers la mer, mais présente des directions variables dictées par les axes gravitaires de plus grande pente, puis par des basculements post-sédimentaires d'origine tectonique. Par ailleurs, des lambeaux résiduels d'une surface d'érosion renfermant des blocs, des galets ou des nummulites oxydées de l'Éocène, ou encore des microfaunes allant de l'Oligocène jusqu'au Miocène moyen pourraient correspondre à la surface d'érosion messinienne. Cette marge est-sarde est cependant atypique dans la mesure où les premiers témoins d'une transgression n'apparaissent qu'à partir du Pliocène supérieur.
13ème Congrès Français de Sédimentologie
Dijon, France

hal-00657312
https://hal-brgm.archives-ouvertes.fr/hal-00657312

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Veterinary pharmaceuticals, widely used in intensive livestock production, may contaminate surface waters. Identifying their sources and pathways in watersheds is difficult because i) most veterinary pharmaceuticals are used in human medicine as well and ii) septic or sewer wastewater treatment plants (WWTP) can release pharmaceuticals into surface water, even in agricultural headwater watersheds. This study aimed to analyze the spatiotemporal variability of animal-specific, mixed-use, and human-specific pharmaceuticals, from agricultural headwaters with intensive livestock production and a WWTP to a watershed used for Water Framework Directive monitoring. Grab sampling was performed during one hydrological year upstream and downstream from a WWTP and at three dates in seven nested watersheds with areas of 1.9-84.1km2. Twenty pharmaceuticals were analyzed. Animal-specific pharmaceuticals were detected at all sampling dates upstream and downstream from the WWTP and at concentrations higher than those of human-specific pharmaceuticals. The predominance of animal-specific and mixed-use pharmaceuticals vs. human-specific pharmaceuticals observed at these sampling points was confirmed at the other sampling points. Animal-specific pharmaceuticals were detected mainly during runoff events and periods of manure spreading. A large percentage of mixed-use pharmaceuticals could come from animal sources, but it was difficult to determine. Mixed-use and human-specific pharmaceuticals predominated in the largest watersheds when runoff decreased. In areas of intensive livestock production, mitigation actions should focus on agricultural headwater watersheds to decrease the number of pathways and the transfer volume of veterinary pharmaceuticals, which can be the main contaminants
ISSN: 0048-9697

insu-01574739
https://hal-insu.archives-ouvertes.fr/insu-01574739
DOI : 10.1016/j.scitotenv.2017.07.206
PUBMED : 28783916

Éditeur(s) : Elsevier
Résumé : In May 1997, some white lesions evoking the white spot syndrome disease were observed in Litopenaeus stylirostris broodstock in New Caledonia. The occurrence of these lesions was neither associated with mortality, nor with histological evidence of white spot syndrome baculovirus (WSBV), The evidence suggests that these lesions result from a form of bacterial disease and are associated with an increased bacterial flora on the outer surface of the cuticle, as well as an increased incidence throughout the molt cycle. A microscopic observation of these lesions allows them to be differentiated from WSBV-associated lesions.
Aquaculture (0044-8486) (Elsevier), 2000-03 , Vol. 183 , N. 1-2 , P. 25-30

Droits : 2000 Elsevier Science B.V. All rights reserved
http://archimer.ifremer.fr/doc/2000/publication-373.pdf
DOI:10.1016/S0044-8486(99)00284-7
http://archimer.ifremer.fr/doc/00000/373/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Nanotribological properties of NbSe2 are studied using an atomic friction force microscope. The friction force is measured as a function of normal load and scan speeds ranging from 10 nm s−1 to 40 μm s−1 under two atmospheres (air and argon). At low speed, no effect of atmosphere is noticed and a linear relationship between the friction and normal forces is observed leading to a friction coefficient close to 0.02 for both atmospheres. At high speed, the tip/surface contact obeys the JKR theory and the tribological properties are atmosphere dependent: the shear stress measured in air environment is three times lower than the one measured under argon atmosphere. A special attention is paid to interpret these results through numerical data obtained from a simple athermal model based on Tomlinson approach.
ISSN: 1023-8883

hal-00780496
https://hal.archives-ouvertes.fr/hal-00780496
https://hal.archives-ouvertes.fr/hal-00780496/document
https://hal.archives-ouvertes.fr/hal-00780496/file/Delbe_6345.pdf
DOI : 10.1007/s11249-008-9400-8

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The dynamics of mantle plumes and the origin of their associated swells remain some of the most controversial topics in geodynamics. Here we construct a numerical model of the mantle flow beneath the French Polynesia region. Our study is based on a new regional seismic tomography model, which high resolution allows obtaining information at the scale of plumes. We find excellent correlations between the observed and the modeled dynamic swells, between the modeled flow pattern and the active volcanism and between the buoyancy fluxes obtained from our numerical model and the ones deduced from the swells morphology. These outstanding fits reveal for the first time that a direct link exists between the surface observations and mantle flows.
ISSN: 0094-8276

hal-01236157
http://hal.univ-reunion.fr/hal-01236157
http://hal.univ-reunion.fr/hal-01236157/document
http://hal.univ-reunion.fr/hal-01236157/file/Adam_South_Pacific_Superswell_GRL2010.pdf
DOI : 10.1029/2010GL042534

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We compare a global compilation of shear-wave splitting measurements with azimuthal seismic anisotropy parameters inferred from surface-wave tomography. The currently available splitting dataset is taken from a novel comprehensive collection of available publications that is updated interactively online. The comparison between the two types of data is made by calculating predicted splitting parameters from the anisotropic tomography model. Comparing these predicted splitting parameters with the observed ones, we find a considerable correlation between the two datasets at global scale. This result is noteworthy, since such correlation did not seem to exist in previous studies. The spatial resolution associated with the two types of methods is rather different. While surface waves have good vertical resolution and poor lateral resolution of several hundreds of kilometers, SKS splitting measurements have good lateral, but poor vertical resolution. The correlation can be understood in light of recent propositions that anisotropy seen by SKS splitting constrains mostly the upper mantle, and therefore a similar depth region as surface waves. The correlation also confirms the generally good quality of the shear-wave measurements, as well as that of the anisotropic tomography model.
ISSN: 0031-9201

hal-00429396
https://hal.archives-ouvertes.fr/hal-00429396
https://hal.archives-ouvertes.fr/hal-00429396/document
https://hal.archives-ouvertes.fr/hal-00429396/file/Wuestefeld_SKS_SW_correlation_PEPI2009-hal.pdf
DOI : 10.1016/j.pepi.2009.05.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Trace metals (Cd, U, Co, Ni, Cu, Ba, Fe, Mn), total organic carbon (TOC) and C and N stable isotope signatures (delta C-313 and delta N-15) were determined in short sediments cores from the inner and outer shelf off Concepcion, Chile (similar to 36 degrees S). The objectives were to establish the effect of environmental conditions on trace metal distributions at two shelf sites, one affected by seasonal oxygenation and the other by permanent low oxygen conditions due to the presence of the oxygen minimum zone (OMZ). We evaluate trace metals as proxies of past changes in primary productivity and the bottom water oxygen regime. Concentrations of pore water sulfides and NH4+ were also measured as indicators of the main diagenetic pathways at each site. Our results for the inner shelf (seasonal suboxia) suggest that the oxidative state of the sediments responds to seasonal pulses of organic matter and that seasonal oxygenation develops during high and low primary productivity in the water column. Here, positive fluxes (to the water column) estimated from pore water concentrations of several elements were observed (Ba, Co, Ni, Fe and Mn). The less reduced environment at this site produces authigenic enrichment of Cu associated with the formation of oxides in the oxic surface sediment layer, and the reduction of U within deeper sediment sections occur consistently with negative estimated pore water fluxes. In the outer shelf sediments (permanent suboxia, OMZ site), negative fluxes (to the sediment) were estimated for all elements, but these sediments showed authigenic enrichments only for Cd, Cu and U. The short oxygenation period during the winter season did not affect the accumulation of these metals on the shelf. The distribution of Cu, Cd and U have been preserved within the sediments and the authigenic accumulation rates estimated showed a decrease from the deep sections of the core to the surface sediments. This could be explained by a gradual decrease in the strength of the OMZ in the last 100 years, in combination with periods of strong oxygenations during El Nino events. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN: 0278-4343

hal-00745672
https://hal.archives-ouvertes.fr/hal-00745672
DOI : 10.1016/j.csr.2011.11.006

Éditeur(s) : HAL CCSD
Résumé : Staphylococcus aureus can cause a wide range of infections in humans and warm-blooded animals. The epithelial surface is the principal site of staphylococcal infection. The goal of this work was to investigate the cytopathic effect of S. aureus and its ability to affect host cell cycle. Cultures of human cervix cancer HeLa and mammary gland bovine MAC-T epithelial cells were used to study in vitro the effects of S. aureus strains from human or ruminant origins. Using microscopic examination, we found that S. aureus induced an enlargement of the host cells and we observed that S. aureus slowed down cell proliferation. Similar effect was observed on both human and bovine cells using the human or the ruminant strains. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infected HeLa and Mac-T cells, synchronized by Double Thymidine Block. Flow cytometry analysis showed that S. aureus induces G2/M phase delay in synchronous HeLa cells. Western blot analysis revealed that the G2/M phase delay was associated with an accumulation of inactive phosphorylated cyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of core histone H3, an important component of the epigenetic landscape. Altogether, these results suggest that the delay in G2/M phase transition of the infected epithelial cells may be one of the mechanisms employed by S. aureus to survive and to propagate inside the host. Further investigations are ongoing to fully understand the mechanisms associated to this type of S. aureushost cell interactions.
journées des Microbiologistes de l' INRA 2012
L'Isle sur la Sorgue, France

hal-01209365
https://hal.archives-ouvertes.fr/hal-01209365
PRODINRA : 174569

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Tectonically controlled landforms develop morphologic features that provide useful markers to investigate crustal deformation and relief growth dynamics. In this paper, we present results of morphotectonic experiments obtained with an innovative approach combining tectonic and surface processes (erosion, transport, and sedimentation), coupled with accurate model monitoring techniques. This approach allows for a qualitative and quantitative analysis of landscape evolution in response to active deformation in the three end-member geological settings: compression, extension, and strike–slip.Experimental results outline first that experimental morphologies evolve significantly at a short time scale. Numerous morphologic markers form continuously, but their lifetime is generally short because erosion and sedimentation processes tend to destroy or bury them. For the compressional setting, the formation of terraces above an active thrust appears mainly controlled by narrowing and incision of the main channel through the uplifting hanging-wall and by avulsion of deposits on fan-like bodies. Terrace formation is irregular even under steady tectonic rates and erosional conditions. Terrace deformation analysis allows retrieving the growth history of the structure and the fault slip rate evolution. For the extensional setting, the dynamics of hanging-wall sedimentary filling appears to control the position of the base level, which in turn controls footwall erosion. Two phases of relief evolution can be evidenced: the first is a phase of relief growth, and the second is a phase of upstream propagation of topographic equilibrium that is reached first in the sedimentary basin. During the phase of relief growth, the formation of triangular facets occurs by degradation of the fault scarp, and their geometry (height) becomes stationary during the phase of upstream propagation of the topographic equilibrium. For the strike–slip setting, the complex morphology of the wrench zone, composed of several interacting fault segments, enhances the interactions with the drainage network. Because of the widening of the main fault zone toward the surface, a significant amount of distributed deformation is observed along the wrench zone. Locally, where two terminations of fault segments interact, less than a quarter of the far field displacement can remain measurable using fault offsets, leading to a systematic underestimation of the real fault slip rate.These different experimental examples illustrate the great potential of the approach coupling deformation mechanisms and erosion–transport–sedimentation processes to investigate qualitatively and quantitatively the morphotectonic evolution of tectonically controlled landscapes.
ISSN: 0169-555X

hal-01243587
https://hal.archives-ouvertes.fr/hal-01243587
DOI : 10.1016/j.geomorph.2015.02.011

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : 51 pages; 15figures; 3 tables, accepted for publication in PloS ONE
International audience
We present a study about AFM imaging of living, moving or self-immobilized, bacteria in their genuine physiological liquid medium. No external immobilization protocol, neither chemical nor mechanical, was needed. For the first time, the native gliding movements of Gram-negative Nostoc cyanobacteria upon the surface, at speeds up to 900microns/h, were studied by AFM. This was possible thanks to an improved combination of a gentle sample preparation process and an AFM procedure based on fast and complete force-distance curves made at every pixel, drastically reducing lateral forces. No limitation in spatial resolution or imaging rate was detected. Gram-positive and non-motile R. wratislaviensis bacteria were studied as well. From the approach curves, Young modulus and turgor pressure were measured for both strains at different gliding speeds and are ranging from 20±3 to 105±5MPa and 40±5 to 310±30kPa depending on the bacterium and the gliding speed. For Nostoc, spatially limited zones with higher values of stiffness were observed. The related spatial period is much higher than the mean length of Nostoc nodules. This was explained by an inhomogeneous mechanical activation of nodules in the Nostoc. We also observed the presence of a soft extra cellular matrix (ECM) around the Nostoc. Both strains left a track of polymeric slime with variable thicknesses. For R. wratislaviensis, it is equal to few hundreds of nanometers, likely to promote its adhesion to the sample. While gliding, the Nostoc secretes a slime layer the thickness of which is in the nanometer range and increases with the gliding speed.
ISSN: 1932-6203

hal-00769765
https://hal.archives-ouvertes.fr/hal-00769765
https://hal.archives-ouvertes.fr/hal-00769765v3/document
https://hal.archives-ouvertes.fr/hal-00769765/file/REsubmission_In-situ_determination_Dhahri_Plos_One_R2_Hal.pdf

Éditeur(s) : HAL CCSD
Résumé : Staphylococcus aureus can cause a wide range of infections in humans and warm-blooded animals. The epithelial surface is the principal site of staphylococcal infection. The goal of this work was to investigate the cytopathic effect of S. aureus and its ability to affect host cell cycle. Cultures of human cervix cancer HeLa and mammary gland bovine MAC-T epithelial cells were used to study in vitro the effects of S. aureus strains from human or ruminant origins. Using microscopic examination, we found that S. aureus induced an enlargement of the host cells and we observed that S. aureus slowed down cell proliferation. Similar effect was observed on both human and bovine cells using the human or the ruminant strains. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infected HeLa and Mac-T cells, synchronized by Double Thymidine Block. Flow cytometry analysis showed that S. aureus induces G2/M phase delay in synchronous HeLa cells. Western blot analysis revealed that the G2/M phase delay was associated with an accumulation of inactive phosphorylated cyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of core histone H3, an important component of the epigenetic landscape. Altogether, these results suggest that the delay in G2/M phase transition of the infected epithelial cells may be one of the mechanisms employed by S. aureus to survive and to propagate inside the host. Further investigations are ongoing to fully understand the mechanisms associated to this type of S. aureus-host cell interactions.
International Symposium on Staphylococci and Staphylococcal Infections -ISSSI-2012
Lyon, France

hal-01209356
https://hal.archives-ouvertes.fr/hal-01209356
PRODINRA : 173265

Résumé : Le fluide chaud produit par les forages de la centrale géothermique de Bouillante (Guadeloupe) est enrichi en espèces dissoutes telles que la silice qui peuvent précipiter sous forme de dépôts solides lors de son refroidissement et gêner fortement la production électrique. L’un des principaux objectifs de ce travail de thèse a donc été d’améliorer l’état des connaissances sur les conditions physiques, chimiques et cinétiques de la précipitation de la silice amorphe à Bouillante. Après une étude des caractéristiques physico-chimiques des fluides et des dépôts associés au champ de Bouillante, des expériences sur la cinétique de précipitation de la silice, sur site, pour une large gamme de température (25 - 90°C) et de pH (4 - 12) ont été menées et analysées en détail.Pour l’ensemble de ces expériences, seule la polymérisation (réversible) de la silice dissoute, sous forme de suspensions colloïdales très stables en solution, a été observée. La modélisation des données expérimentales de cinétique montre que la vitesse de polymérisation de la silice suit une loi d’ordre 2 vis-à-vis de la silice dissoute jusqu’à l’équilibre en accord avec une dimérisation de la silice. Par contre, durant l’étape initiale de la réaction, une loi d’ordre 4 semble mieux adaptée, suggérant des réactions de polymérisation plus complexes avec formation de tétramères. La température a un effet limité sur la cinétique de polymérisation de la silice comparé à l’effet du pH qui est beaucoup plus important. L’énergie d’activation de la réaction globale est de 41 - 54 kJ pour des pH compris entre 5 et 8.La caractérisation de la silice précipitée a mis en évidence des différences notables entre la structure poreuse de la silice solide prélevée sur les installations de surface et celle de la silice colloïdale obtenue par polymérisation lors des expériences de cinétique, ce qui semble indiquer que des mécanismes différents interviennent dans ces deux contextes de précipitation de la silice dissoute.Le deuxième objectif de cette thèse était d’évaluer la possibilité de valoriser la silice extraite de l’eau géothermale de Bouillante dans un procédé de traitement des eaux naturelles par adsorption. Pour cela, les propriétés d’adsorption de la silice géothermale brute et fonctionnalisée par le poly(ethylèneimine) (ou PEI) ont été étudiées pour deux polluants : un colorant, le bleu de méthylène et un métal lourd, le plomb. Les résultats obtenus montrent que la silice brute possède une grande capacité d’adsorption vis-à-vis du bleu de méthylène. En outre, la fonctionnalisation de la silice par le PEI permet d’améliorer sa capacité d’adsorption vis-à-vis du plomb en solution, en accord avec un modèle cinétique du pseudo-second ordre suggérant une chimisorption. Le modèle d’isotherme de Redlich-Peterson est également en très bon accord avec les données expérimentales.
The hot geothermal fluid produced by the geothermal power plant of Bouillante (Guadeloupe) contains various dissolved components such as silica that can precipitate as solid deposits during their cooling and cause serious problems on the electricity generation. One of the aim of this study is to improve the understanding on the physical, chemical and kinetics conditions of the amorphous silica precipitation in the Bouillante geothermal field. After a study on the physical and chemical characteristics of the Bouillante fluids and deposits which can form during their exploitation, experiments on the kinetics of silica precipitation, on site, under a large range of temperature (25 – 90°C) and pH (4 – 12) have been carried out and analyzed in detail.During on site kinetics experimentations only dissolved silica polymerization (reversible) as colloidal gel, very stable in solution, was observed. Modeling of the experimental data showed that the kinetics polymerization is characterized by a 2-order kinetic law relative to dissolved silica until the equilibrium that is consistent with silica dimerization. On the other side, the initial stage of the reaction is better characterized by a 4-order kinetic law that suggested more complex polymerization reactions with tetramers formation.Temperature has a little effect on the polymerization kinetics compared with the pH which has an effect more important. The activation energy of silica precipitation calculated between 25 and 90°C, is about 41 - 54 kJ for pH ranging to 5 - 8.The characterization of the silica precipitate reveals significant differences in the porous structure of the silica collected on the surface installations and the one obtained from kinetic experiments that suggests different precipitation mechanisms. The second objective of this thesis was to evaluate the possibility to promote geothermal silica for the treatment of natural waters containing contaminants by adsorption. Adsorption properties of the untreated and functionalized by poly(ethylèneimine) (ou PEI) geothermal silica have been investigated for two pollutants: a colorant, the methylene blue and a heavy metal, the lead.Results showed that untreated silica has a great adsorption capacity concerning the methylene blue. Besides, silica functionalization by the PEI allows improving the adsorption capacity for lead in solution. The adsorption kinetics curves followed a pseudo-second order model consistent with a chimisorption. The isotherm model of Redlich-Peterson fitted well the experimental data.

http://www.theses.fr/2014AGUY0698/document

Éditeur(s) : HAL CCSD
Résumé :  
The gelatinous layer (G-layer) of tension wood fibres in hardwood contributes to the mechanical function of the living tree and has significant consequences on properties of solid wood. Its size, shape and structure observed by optical or electron microscopy exhibits characteristic anatomical features. However, we found that sectioning of non-embedded wood samples results in an uncontrolled swelling of the G-layer was monitored by serial sections, from an embedded wood sample, from its trimmed transverse face to that located several hundreds of micrometers deep. The results revealed that the initial cutting before embedding produced a border effect responsible for the swollen nature, which is similar to sections from non-embedded material. After a conventional embedding technique was applied, a section of at least 30 micrometres below the trimming surface is required to observe an un-swollen G-layer.
IAWA Journal

hal-01032054
https://hal.archives-ouvertes.fr/hal-01032054

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The present study aims at better deciphering the different mechanisms involved in the functioning of the subduction interplate. A 2D thermo-mechanical model is used to simulate a subduction channel, made of oceanic crust, free to evolve. Convergence at constant rate is imposed under a 100 km thick upper plate. Pseudo-brittle and non-Newtonian behaviours are modelled. The influence of the subduction channel strength, parameterized by the difference in activation energy between crust and mantle () is investigated to examine in detail the variations in depth of the subduction plane down-dip extent, . First, simulations show that numerical resolution may be responsible for an artificial and significant shallowing of if the weak crustal layer is not correctly resolved. Second, if the age of the subducting plate is 100 Myr, subduction occurs for any . The stiffer the crust is, that is, the lower is, the shallower is (60 km depth if kJ/mol) and the hotter the fore-arc base is. Conversely, imposing a very weak subduction channel ( J/mol) leads there to an extreme mantle wedge cooling and inhibits mantle melting in wet conditions. Partial kinematic coupling at the fore-arc base occurs if kJ/mol. If the incoming plate is 20 Myr old, subduction can occur under the conditions that the crust is either stiff and denser than the mantle, or weak and buoyant. In the latter condition, cold crust plumes rise from the subduction channel and ascend through the upper lithosphere, triggering (1) partial kinematic coupling under the fore-arc, (2) fore-arc lithosphere cooling, and (3) partial or complete hindrance of wet mantle melting. then ranges from 50 to more than 250 km depth and is time-dependent if crust plumes form. Finally, subduction plane dynamics is intimately linked to the regime of subduction-induced corner flow. Two different intervals of are underlined: 80–120 kJ/mol to reproduce the range of slab surface temperature inferred from geothermometry, and 10–40 kJ/mol to reproduce the shallow hot mantle wedge core inferred from conditions of last equilibration of near-primary arc magmas and seismic tomographies. Therefore, an extra process controlling mantle wedge dynamics is needed to satisfy simultaneously the aforementioned observations. A mantle viscosity reduction, by a factor 4–20, caused by metasomatism in the mantle wedge is proposed. From these results, I conclude that the subduction channel down-dip extent, , should depend on the subduction setting, to be consistent with the observed variability of sub-arc depths of the subducting plate surface.
ISSN: 0031-9201

hal-01623155
https://hal.archives-ouvertes.fr/hal-01623155
DOI : 10.1016/j.pepi.2017.05.008

Éditeur(s) : HAL CCSD
Résumé : National audience
La géologie a beaucoup évolué au cours des deux derniers siècles. On analyse l'histoire des géosciences autour du pôle universitaire montpelliérain, à travers l'évolution de la perception des paysages (illustré par l'exemple de la région du Pic Saint Loup) et de la connaissance géologique qui en découle. Les précurseurs ont d'abord établi la succession stratigraphique des roches, puis ils ont commencé de reporter les observations de surface sur des cartes. Les géologues ont ensuite tenté de comprendre les structures du sous-sol, d'abord à l'aide de coupes et plus récemment grâce à des représentations tridimensionnelles.
Annales SHHNH

hal-00933871
https://hal.archives-ouvertes.fr/hal-00933871
https://hal.archives-ouvertes.fr/hal-00933871/document
https://hal.archives-ouvertes.fr/hal-00933871/file/200ansGeolSHHNH.pdf