Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The behaviour of the four Ra isotopes (measured by gamma spectrometry) and 222Rn (measured with an AlphaGUARD ionisation chamber) has been investigated in NaCl-rich thermal waters of Balaruc-les-Bains on the Mediterranean coast (South of France). This study allows identification of the deep thermal water signature and reveals the influence of seawater or karst water inflows on Ra isotopes and 222Rn. The deep thermal water has 226Ra and 222Rn activities of 840 and 1900 mBq/l, respectively, a (228Ra/226Ra) activity ratio of 0.59, and low (224Ra/228Ra) and (223Ra/226Ra) ratios of 0.67 and 0.025, respectively. Several arguments suggest a dominant role of radioactive decay for short-lived Ra isotopes and Rn during the relatively rapid ascent of thermal water through wide open fractures and drains. The low (223Ra/226Ra) ratio constrains the maximum ascent velocity of thermal water to 8-10 m/h. Seawater inflow into the hydrothermal system results in an enrichment in short-lived Ra isotopes and 222Rn. The high (223Ra/226Ra) ratio (0.23) suggests that 223Ra, 224Ra and 222Rn produced by alpha-recoil in the upper-Jurassic limestone are entrained by seawater percolation through the coastal basement, due to active pumping in one of the near shore production well. This process would be enhanced by a low water/rock ratio (i.e. in a low-porosity, micro-fractured limestone). Mixing of this enriched seawater with thermal water induces barite precipitation with co-precipitation of a large fraction of Ra. Short-lived Ra isotopes may thus be useful tracers of seawater flux towards the continent. Conversely, the inflow and mixing of karst groundwater result in a general dilution of all dissolved elements, only a small enrichment in 224Ra and 223Ra, but a large enrichment in 222Rn (up to 26 Bq/l). The combination of Ra isotopes and 222Rn data provides a good way to trace the dynamics of different water masses along coastal areas.
ISSN: 0016-7037

hal-00795512
https://hal.archives-ouvertes.fr/hal-00795512
DOI : 10.1016/j.gca.2012.09.010

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We provide results of a detailed study of the first peridotite xenoliths of proven mantle origin reported from Bezymyanny volcano in the Klyuchevskoy Group, northern Kamchatka arc. The xenoliths are coarse spinel harzburgites made up mainly of Mg-rich olivine as well as subhedral orthopyroxene (opx) and Cr-rich spinel, and also contain fine-grained interstitial pyroxenes, amphibole and feldspar. The samples are unique in preserving the evidence for both initial arc mantle substrate produced by high-degree melt extraction and subsequent enrichment events. We show that the textures, modal and major oxide compositions of the Bezymyanny xenoliths are generally similar to those of spinel harzburgite xenoliths from Avacha volcano in southern Kamchatka. However, coarse opx from the Bezymyanny harzburgites has higher abundances of light and medium rare earth elements and other highly incompatible elements than coarse opx from the Avacha harzburgites. We infer that (1) the sub-arc lithospheric mantle beneath both Avacha and Bezymyanny (and possibly between these volcanoes) consists predominantly of harzburgitic melting residues, which experienced metasomatism by slab-related fluids or low-fraction, fluid-rich melts and (2) the degrees of metasomatism are higher beneath Bezymyanny. By contrast, xenolith suites from Shiveluch and Kharchinsky volcanoes 50-100 km north of the Klyuchevskoy Group include abundant cumulates and products of reaction of mantle rocks with silicate melts at high melt/rock ratios. The high melt flux through the lithospheric mantle beneath Shiveluch and Kharchinsky may be related to the asthenospheric flow around the northern edge of the sinking Pacific plate; lateral propagation of fluids in the mantle wedge south of the plate edge may contribute to metasomatism in the mantle lithosphere beneath the Klyuchevskoy Group volcanoes.
ISSN: 0377-0273

hal-00941258
https://hal.archives-ouvertes.fr/hal-00941258
DOI : 10.1016/j.jvolgeores.2012.12.022

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mantle noble gases record important and ancient isotopic heterogeneities, which fundamentally influence our understanding of mantle geodynamics, yet these heterogeneities are difficult to fully interpret without understanding the basic mechanisms of noble gas storage and transport in mantle minerals. A series of annealing experiments that mimic mantle conditions (i.e. sub-solidus with natural, polycrystalline, texturally equilibrated olivines at low noble gas partial pressures) show that intergranular interfaces (grain boundaries) are major hosts for noble gases in the mantle, and that interfaces can dramatically fractionate noble gases from their radio-parents (U + Th and K). Therefore, noble gas isotopic heterogeneities in the mantle could result from grain size variations. Fine-grained lithologies (mylonites and ultramylonites, for example) with more grain boundaries will have lower U/3He ratios (compared to a coarse grained equivalent), which, over time, will preserve higher 3He/4He ratios. As predicted by theory of points defect diffusivity, these results show that noble gas diffusion along interfaces is different from those in the grain lattice itself at low temperatures. However, for grain size relevant of the Earth's mantle, the resulting effective correlated activation energies (Ea)(Ea) and pre-exponential factors (Do/a2)(Do/a2) produce similar diffusivities at mantle temperatures for interface- and lattice-hosted helium. Therefore, grain boundaries do not significantly affect helium transport at mantle conditions and length scales.
ISSN: 0012-821X

hal-01236013
https://hal.archives-ouvertes.fr/hal-01236013
DOI : 10.1016/j.epsl.2015.08.024

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Vertebrate genomes contain around 20,000 protein-encoding genes, of which a large fraction is still not associated with specific functions. A major task in future genomics will thus be to assign physiological roles to all open reading frames revealed by genome sequencing. Here we show that C2orf62, a highly conserved protein with little homology to characterized proteins, is strongly expressed in testis in zebrafish and mammals, and in various types of ciliated cells during zebrafish development. By yeast two hybrid and GST pull-down, C2orf62 was shown to interact with TTC17, another uncharacterized protein. Depletion of either C2orf62 or TTC17 in human ciliated cells interferes with actin polymerization and reduces the number of primary cilia without changing their length. Zebrafish embryos injected with morpholinos against C2orf62 or TTC17, or with mRNA coding for the C2orf62 C-terminal part containing a RII dimerization/docking (R2D2) - like domain show morphological defects consistent with imperfect ciliogenesis. We provide here the first evidence for a C2orf62-TTC17 axis that would regulate actin polymerization and ciliogenesis.
ISSN: 1932-6203

hal-00955242
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00955242
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00955242/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00955242/file/C2orf62_and_TTC17-accepted.pdf
DOI : 10.1371/journal.pone.0086476

Résumé : If Sea Bass fish farming (Dicentrarchus labrax L.) is currently experiencing an increase in Southern Europe, it is vital, in order to better reflect raising structures, to control its wastes. In order to do so, quantifying this species' wastes is essential to be able to present simple excretion ratios, easily useable by the farmer. Those ratios have been based on nitrogen, phosphorus and carbon masses established on intensive farming and 4 batches belonging to his stock (averaging 24, 5 to 362, 5 g). Each batch was followed up during the course of 1 month and fish farming in its entirety for 2 months. Mass assessments for nitrogen and phosphorus were level ((intrants = extrants) but never precise, contrary to what can be found in bibliography. The main wastes defined were linked to the biomasses found as well as the food quantity fed. Sea Bass carbon excretion could not be clearly defined. According to these follow ups, phosphorus would be the element better utilized by Sea Bass considering nutritional provision. Nitrogen, phosphorus and carbon quantities rejected through farming are very high since for nitrogen and carbon, 90% of the ingestion would be rejected in the environment.
INTRODUCTION : Les pêcheries de langoustine de Mer Celtique se situent essentiellement dans les subdivisions Vllg et Vllh. La réglementation communautaire sur les maillages à langoustine y a été appliquée à partir de 1978. L'emploi d'un maillage dérogataire pour la pêche de la langoustine n'est possible que si le pourcentage d'espèces protégées par une taille marchande pêchées en même temps que la langoustine est inférieur à un seuil déterminé en relation avec le maillage en vigueur. L'application de cette réglementation s'est effectuée suivant un calendrier particulier Avec un pourcentage d'espèces protégées supérieur à 60 %, la pêche est considérée comme dirigée vers le poisson et le maillage est celui de la réglementation générale, soit 80 mm. En fait, comme cela a été mentionné à maintes reprises, sous la pression de cette réglementation contraignante, les flottilles et les techniques de pêche ont évolué très rapidement si bien que le pouvoir de capture de ces navires, aussi bien sur la langoustine que sur le poisson, a considérablement augmenté. Dans la majorité des cas, la flexibilité est devenue la règle et l'adoption du maillage de 80 mm tend à se généraliser. Ces navires s'orientent toujours momentanément vers la pêche la plus rentable, la langoustine demeurant l'espèce-cible, même si elle ne constitue pas toujours pondéralement l'essentiel des captures. Un des reproches majeurs fait aux langoustiniers de Mer Celtique était la destruction possible d'immatures de poissons. Une évaluation de ces rejets a été réalisée en 1980, soit donc avec un maillage situé entre 55 et 60 mm. Pour la plupart des espèces, les rejets sont anecdotiques en raison vraisemblablement d'une distribution spatiale différente des immatures et des adultes. Dans le cas du merlan, les immatures sont concentrés dans les zones côtières en hiver et ne sont capturés que si les langoustiniers sont amenés à abandonner la zone des bancs centraux en raison, en particulier, du mauvais temps. Seuls le merlu et la cardine présentent des pourcentages de rejets très importants car ils sont inféodés aux mêmes fonds vaseux que la langoustine, et leurs migrations trophiques, s'il en existe, ont lieu dans ces aires de dépôts sédimentaires au pied des hauts fonds. Dans ces premières évaluations, le volume observé des rejets de merlu étaient pour le moins inquiétant et laissait supposer, soit qu'il existait sur le fond au moment du recrutement des quantités énormes d'immatures soit que la sélectivité du chalut était abaissée par la présence de la langoustine. Ce deuxième point a été vérifié depuis et il semble que la sélectivité du merlu avec ou sans chalut à langoustine soit strictement la même. Les rejets de langoustine n'ont pas été comptabilisés car les valeurs auraient été inexploitables en raison de la différence entre la taille marchande communautaire, soit 25 mm de longueur céphalothoracique et la taille française soit 34, 5 mm. Ils seraient respectivement de 1, 75 % en nombre dans le premier cas contre 55 % dans le deuxième. En raison des modifications importantes intervenues dans l'aménagement des pêches dans cette zone, il était indispensable de refaire une évaluation pondérale des rejets : Pour obtenir des compositions en taille des espèces ne se prêtant pas aux analyses habituelles sur les âges. Pour calculer des courbes de tri manuel sur les espèces dont il est difficile d'évaluer les rejets en routine, mais seulement dans la mesure où les rejets se superposent à la fraction commerciale de la capture.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1985/rapport-1753.pdf
http://archimer.ifremer.fr/doc/00000/1753/

Éditeur(s) : HAL CCSD American Physical Society
Résumé : International audience
We investigate a class of granular materials characterized by the possibility of interlocking between the particles. The interlocking is modeled by its effect through rolling resistance depending on relative rotation and normal force at the contact points and involving a single parameter analogous to the sliding friction coefficient. The model, which is introduced in the framework of the contact dynamics, method, is applied to simulate the simple shear of a large granular sample. We present a detailed analysis regarding the influence of rolling and sliding friction parameters on the macroscopic response in terms of shear strength, fabric properties, and force transmission. Interestingly, two distinct regimes can be distinguished in which the steady-state shear strength is controlled by either rolling resistance or sliding friction. The relative contributions of rolling and sliding contacts to the shear strength are consistent with the same two regimes. Interlocking strongly affects the force network by enhancing the arching effect and thus increasing the relative importance of weak contact forces and torques, which is reflected in a decreasing power-law probability distribution of the contact forces and torques below the mean. Due to the combined effect of friction and interlocking, the force-carrying backbone takes an increasingly columnar aspect involving a low fraction of particles. Our data suggest that the nature of the weak contact network is strongly affected by the formation of these columns of particles which do not need to be propped laterally. In particular, in the limit of high rolling resistance and sliding friction, the role of the weak network of contacts is no longer to prop the force chains, but, like the strong contact network, to actively sustain the deviatoric load imposed on the system.
ISSN: 1539-3755

hal-00412020
https://hal.archives-ouvertes.fr/hal-00412020
DOI : 10.1103/PhysRevE.78.021301

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together, while communities from the same site but different seasons were unrelated. Both DNA and RNA-based surveys provided similar relative abundances for most class-level taxonomic groups. Yet, particular groups were overrepresented in one of the two templates, such as marine alveolates (MALV)-I and MALV-II that were much more abundant in DNA surveys. Overall, the groups displaying the highest relative contribution were Dinophyceae, Diatomea, Ciliophora and Acantharia. Also, well represented were Mamiellophyceae, Cryptomonadales, marine alveolates and marine stramenopiles in the picoplankton, and Monadofilosa and basal Fungi in sediments. Our extensive and systematic sequencing of geographically separated sites provides the most comprehensive molecular description of coastal marine protist diversity to date.
ISSN: 1462-2912

hal-01191731
http://hal.upmc.fr/hal-01191731
http://hal.upmc.fr/hal-01191731/document
http://hal.upmc.fr/hal-01191731/file/Massana_2015_Marine_protist.pdf
DOI : 10.1111/1462-2920.12955

Éditeur(s) : American Geophysical Union
Résumé : The origins and fates of the Atlantic salinity maximum water (SMW), formed through excess evaporation in the tropics and subtropics of both hemispheres, are studied using monthly mean outputs of a numerical simulation of the world ocean climatological circulation. After defining formation domains from the surface salinity field and the vertical stratification, a Lagrangian technique is used to estimate the formation rates and main pathways in each hemisphere and the role of this water in the framework of the warm water return flow of the meridional overturning cell. Formation rates around 9 and 11 Sv are found in the Southern and Northern Hemispheres, respectively. While the export of the southern SMW from its formation area is realized by the western boundary currents, that of the northern SMW mainly results from interior subduction. Equatorward of the formation regions, a fraction of each SMW variety is entrained in the subtropical cells that connect the subtropics to the equatorial region. Poleward of them, both varieties are seen to feed the regions of subtropical mode water (STMW) formation around 35degrees of latitude in both hemispheres. The bulk of the transport associated with each variety eventually turns northward: This amounts to similar to6 Sv of southern SMW gathered in the North Brazil Undercurrent (NBUC), and similar to10 Sv of northern SMW found in the Gulf Stream at 35degreesN, of which 8 Sv have gone through the Caribbean Sea. Of the 13.4-Sv northward transport of the meridional overturning cell estimated by the model at 47degreesN, more than 50% (6.9 Sv) is found to have transited through at least one of the SMW regions. This gives an indication of the likely important role of SMW formation in the observed northward salinity increase of the upper Atlantic Ocean.
Journal of Geophysical Union - Research C - Oceans (0148-0227) (American Geophysical Union), 2002-09 , Vol. 107 , N. C10 , P. NIL_610-NIL_624

Droits : 2002 by the American Geophysical Union
http://archimer.ifremer.fr/doc/2002/publication-769.pdf
DOI:10.1029/2002JC001318
http://archimer.ifremer.fr/doc/00000/769/

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Mantle cell lymphoma (MCL) is a hematologic neoplasm characterised by the t(11;14)(q13;q32) translocation leading to aberrant cyclin D1 expression. The cell functions of cyclin D1 depend on its partners and/or subcellular distribution, resulting in different oncogenic properties. We observed the accumulation of cyclin D1 in the cytoplasm of a subset of MCL cell lines and primary cells. In primary cells, this cytoplasmic distribution was correlated with a more frequent blastoid phenotype. We performed immunoprecipitation assays and mass spectrometry on enriched cytosolic fractions from two cell lines. The cyclin D1 interactome was found to include several factors involved in adhesion, migration and invasion. We found that the accumulation of cyclin D1 in the cytoplasm was associated with higher levels of migration and invasiveness. We also showed that MCL cells with high cytoplasmic levels of cyclin D1 engrafted more rapidly into the bone marrow, spleen, and brain in immunodeficient mice. Both migration and invasion processes, both in vivo and in vitro, were counteracted by the exportin 1 inhibitor KPT-330, which retains cyclin D1 in the nucleus. Our data reveal a role of cytoplasmic cyclin D1 in the control of MCL cell migration and invasion, and as a true operator of MCL pathogenesis.
ISSN: 2045-2322

hal-01635754
https://hal.archives-ouvertes.fr/hal-01635754
DOI : 10.1038/s41598-017-14222-1
PUBMED : 29066743

Éditeur(s) : HAL CCSD
Résumé : International audience
This paper deals with image processing. This study takes place in a segmentation process based on texture analysis. We use the multifractal ap-proach to characterize the textures. More precisely we study a particular multi-fractal spectrum called the large deviation spectrum. We consider two statistical methods to numerically compute this spectrum. The resulting spectrum, com-puted by both methods over an image, is a one dimension spectrum. In the scope of this article, we extend these methods in order to obtain a two dimen-sions spectrum which could be assimilated to an image. This 2D spectrum al-lows a local characterization of the image singularities while a 1D spectrum is a global characterization. Moreover, the computation of the spectrum requires the use of a measure. We introduce here a pre processing based on the gradient to improve the measure. We show results on both synthetic and real world images. Finally, we remark that the resulting 2D spectrum is close to the resulting im-age of an edge detection process while edge detection using one dimension spectrum requires post processing methods. This statement will be used for fu-ture works.
SITIS Proceedings
Hammamet, Tunisia

hal-00602273
https://hal.archives-ouvertes.fr/hal-00602273

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
We performed 31 friction experiments on glassy basalts (GB) and glass-free basalts (GFB) at slip rates up to 6.5 m s−1 and normal stress up to 40 MPa (seismic conditions). Frictional weakening was associated to bulk frictional melting and lubrication. The weakening distance (Dw) was about 3 times shorter in GB than in GFB, but the steady state friction was systematically higher in GB than in GFB. The shorter Dw in GB may be explained by the thermal softening occurring at the glass transition temperature (Tg ~500°C), which is lower than the bulk melting temperature (Tm ~1250°C) of GFB. Postexperiment microanalyses suggest that the larger crystal fraction measured in GB melts results in the higher steady state friction value compared to the GFB melts. The effect of interstitial glass is to facilitate frictional instability and rupture propagation in GB with respect to GFB.
ISSN: 0094-8276

hal-01054139
https://hal.archives-ouvertes.fr/hal-01054139
DOI : 10.1002/2013GL058601

Éditeur(s) : HAL CCSD
Résumé : International audience
In real world situations, each person is generally in contact with only a small fraction of the entire population and exchange information through these interactions. Their number and their frequency vary from one to another individual and may be much depending on mobility of individuals. The objective of this article is to better understand how human mobility may have an impact on mobile social networking systems. This should help to answer a question as: "How might an information, a rumor, a pathogen, etc., driven by physical proximity, spread through a population?". We present a first stage of our work in which we focus on percolation processes as information flow mechanisms. We propose a synthetic mobility model and we define an artificial world populated by heterogeneous agents who differ in their mobility. Simulations are conducted on a multi-agent programmable environment. Our experimental results clearly demonstrate positive correlations between agent mobility factors and percolation thresholds.
Procedia CS

hal-00767043
https://hal.archives-ouvertes.fr/hal-00767043
DOI : 10.1016/j.procs.2012.06.007

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mid-ocean ridge basalts (MORBs) are the most abundant magmas produced on Earth. They are widely studied to infer mantle compositions and melting processes. However, MORB liquids are also the complex end-product of a variety of intra-crustal processes such as partial or fractional crystallization, melt–rock interaction, and contamination. Deciphering the relative contribution of these different processes is of first-order importance. Contamination at ocean crustal levels is likely, and may occur at magma chamber margins where fresh magmas can interact with previously hydrothermally altered rocks. Characterizing the composition of this crustal contaminant component is critical if we are to understand the relative importance of each component in the resulting MORB liquid.Here we present the results of experiments designed to reproduce the processes occurring at oceanic magma chamber roofs, where crustal contamination should be most extensive, by melting a representative sample of the sheeted dike complex. Anatectic melts thus produced are likely to represent the principal crustal contaminant in MORB. These melts were characterized for major and trace elements, showing B, Zr, Hf, and U enrichment, and Sr, Ti, and V depletion relative to original MORB liquids. In comparison to the starting material, relative element fractionations are observed in the anatectic melts, with enrichments of: U relative to Ba, Nb, and Th; LREE and MREE relative to Sr; and Zr–Hf relative to LREE. Bulk partition coefficients for element partitioning during magma chamber roof anatexis are derived and proposed as valuable tools for tracking MORB contamination.Comparison with natural samples from the East Pacific Rise and the Oman ophiolite shows that anatectic melts can crystallize in situ to form oceanic plagiogranite intrusions, and that residual assemblages associated with the hydrous partial melting stage are represented by hornfelsic dikes and enclaves (also named granoblastic basalts). We now recognize these as commonplace at the root of the sheeted dike complex both at present-day and fossil oceanic spreading centers.
ISSN: 0024-4937

hal-01115500
https://hal.archives-ouvertes.fr/hal-01115500
DOI : 10.1016/j.lithos.2014.05.018

Éditeur(s) : HAL CCSD
Résumé : International audience
Volumes of magma intruded in and emitted by volcanoes through time can be estimated by reconstruction of vol-cano's morphology and time sequence. Classical approaches for quantifying magma volumes on active volcanoes are based on the difference between pre-and post-eruption digital elevation models (DEM), but this kind of approach needs the pre-eruptive surfaces to be available. For old and eroded volcanoes these surfaces are poorly constrained. However, because the geometrical form of many volcanic edifices exhibits a remarkable symmetry we propose, here, a new approach using primary volcanic landforms in order to estimate the amount of the both erupted and eroded material and to locate eruptive centers. A large fraction of composite volcanoes have near constant slope on their flanks and a form that is concave upwards near their summits. But many phenomena can lead to non-symetrical edifices and complex morphologies can result, for example from parasitic centers of volcanism on the flanks, from alternation of short effusive and explosive construction phases, from flank or caldera collapses, or from glacial and other types of erosion. In this study we propose that, on the first order approximation, complex morphologies can be modeled by piling regular cones. In this model, cones centers and slopes are derived by fitting primary volcanic landform with a linear function :elevation=f(distance from center). Such an approach allows to estimate both errors on location of the eruptive center and on the volume of the resulting cones. This model can then be used for quantifying volume of erupted and eroded material, and for quantifying catastrophic events as giant landslides or flank collapse. This approach is tested on four different active volcanoes : Mount Mayon (Philippines), Mount Fuji (Japan), Mount Etna (Sicily) and Mount Teide (Canary Island) to estimate errors in volume between modeled and actual edifices. It is then used on volcanoes of La Réunion hotspot to reconstruct the Piton des Neiges and Piton de la Fournaise volcanoes at its different stages of growing.
EGU 2014
Vienna, Austria

hal-01386609
http://hal.univ-reunion.fr/hal-01386609

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : Background. In wild plant populations, genetic divergence within continuous stands is common, sometimes at very short geographical scales. While restrictions to gene flow combined with local inbreeding and genetic drift may cause neutral differentiation among subpopulations, microgeographical variations in environmental conditions can drive adaptive divergence through natural selection at some targeted loci. Such phenomena have recurrently been observed in plant populations occurring across sharp environmental boundaries, but the interplay between selective processes and neutral genetic divergence has seldom been studied.[br/]Methods. We assessed the extent of within-stand neutral and environmentally-driven divergence in the Neotropical tree [i]Eperua falcate Aubl[/i]. (Fabaceae) through a genome-scan approach. Populations of this species grow in dense stands that cross the boundaries between starkly contrasting habitats. Within-stand phenotypic and candidate-gene divergence have already been proven, making this species a suitable model for the study of genome-wide microgeographic divergence. Thirty trees from each of two habitats (seasonally flooded swamps and well-drained plateaus) in two separate populations were genotyped using thousands of AFLPs markers. To avoid genotyping errors and increase marker reliability, each sample was genotyped twice and submitted to a rigorous procedure for data cleaning, which resulted in 1196 reliable and reproducible markers.[br/]Results. Despite the short spatial distances, we detected within-populations genetic divergence, probably caused by neutral processes, such as restrictions in gene flow. Moreover, habitat-structured subpopulations belonging to otherwise continuous stands also diverge in relation to environmental variability and habitat patchiness: we detected convincing evidence of divergent selection at the genome-wide level and for a fraction of the analyzed loci (comprised between 0.25% and 1.6%). Simulations showed that the levels of differentiation for these outliers are compatible with scenarios of strong divergent selection
ISSN: 1932-6203

hal-01204220
https://hal.archives-ouvertes.fr/hal-01204220
https://hal.archives-ouvertes.fr/hal-01204220/document
https://hal.archives-ouvertes.fr/hal-01204220/file/journal.pone.0121394.PDF
PRODINRA : 287494

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We report concentrations of hydrogen (H) in upper mantle minerals of peridotites (olivine and pyroxenes) transported by alnöitic lavas, which erupted on the southwestern border of the Ontong Java Plateau (Malaita, Solomon Islands, West Pacific). Unpolarized FTIR analyses show that olivine, orthopyroxene, and diopside contain 2–32 ppm, 162–362 ppm and 159–459 ppm wt H2O, respectively. In the studied lherzolites, garnets are anhydrous. The concentration of hydrogen within individual olivine and pyroxene grains is almost homogeneous, indicating no evidence of dehydration or hydration by ionic diffusion. In the lherzolite, the concentration of hydrogen in olivine tends to increase weakly with depth (based on geothermobarometry), consistent with the increase of water solubility with increasing water fugacity as a function of pressure, but concentrations remain well below water-saturation values determined experimentally. The highest concentration of H in olivine (32 ppm wt H2O) is, however, found in refractory spinel harzburgites, which equilibrated at depths of 85 km., while deeper specimens as the high-temperature spinel harzburgites, and some of the garnet lherzolites, contain less hydrogen in olivine. Olivines from pyroxene- or pargasite-rich peridotites have also lower hydrogen concentrations. We interpret the high hydrogen concentrations in olivine from the refractory spinel harzburgites as due to (1) simultaneous hydration and metasomatism of the lithospheric mantle by a water-rich silicate melt/fluid, during which hydrogen follows MREE and where spinel harzburgite have experienced ‘stealth’ metasomatism, and/or (2) to a late ‘fleeting’ hydrogen metasomatism, which would hydrate the rock after this first ‘stealth’ metasomatism event. In the second case, the composition of the ‘fleeting’ percolating fluid (small volume fraction of very evolved fluids, with high volatiles concentration and transient properties) is likely to be linked to the decrease of the plume activity and resulting in the downwelling of the lithosphere asthenosphere boundary. The difference in hydrogen concentration between harzburgites and lherzolites could be linked to variation in trace elements in olivine induced by the first ‘stealth’ metasomatism alone or associated to the late ‘fleeting’ hydrogen metasomatism, triggered by the lack of secondary crystallization of clinopyroxenes in the harzburgite. To conclude, H concentrations in upper mantle minerals may thus be correlated to metasomatic events, with the later yielding variation of the mineral assemblage and trace element composition that constitute the peridotites.
ISSN: 0024-4937

hal-01143385
https://hal.archives-ouvertes.fr/hal-01143385
DOI : 10.1016/j.lithos.2014.11.005

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In this paper, we have presented a spectral and a multifractal analysis performed on 412 time series of wind speed data each of duration of 350 s and sampled at 20 Hz. The average spectrum for the wind speed displays a scaling behavior, in the inertial range, over two decades, with b ¼ 1:68 close to the Kolmogorov value À 5/3. A multifractal analysis has been motivated by the presence of scaling invariance in data set. Then we have considered their scaling properties in the framework of fully developed turbulence and multifractal cascades. The results obtained for wind speed confirm that the exponent scaling function z V ðqÞ is nonlinear and concave. This exponent characterizes the scaling functions in the inertial range indicating that the wind speed is intermittent and multifractal. Moreover the theoretical quadratic relation for lognormal multifractals is well fitted. We investigate the consequence for wind energy production: we generate stochastic simulations of a multifractal random walk, and using a power curve derived from experimental data, we generate the associated power time series. We show that, due to the saturation of the power curve for large speed values, when the input time series (turbulent wind speed) is multifractal, the output can be almost monofractal.
ISSN: 0167-6105

hal-01527886
https://hal.archives-ouvertes.fr/hal-01527886
DOI : 10.1016/j.jweia.2012.06.002

Éditeur(s) : HAL CCSD Mineralogical Society
Résumé : International audience
Almost all magmas contain sulfide- or sulfate-bearing phases. In most natural samples the sulfur-bearing phase is a sulfide, which typically is pyrrhotite (Fe1−xS) or pyrite (FeS2) although chalcopyrite (CuFeS2), pentlandite ((Fe,Ni)9S8), sphalerite (ZnS) or molybdenite (MoS2) may be present as well. Sulfate minerals are rare at magmatic conditions, and anhydrite (CaSO4) is the most common. Other magmatic SO4-bearing minerals include the sodalite group minerals (haüyne simplified formula: (Na,Ca)4-8(Al6Si6(O,S)24)(SO4,Cl)1-2), scapolite minerals (silvialite: (Ca,Na)4Al6Si6O24(SO4,CO3)), and S-bearing apatite (Ca5(PO4)3(F, Cl, OH)). Barite (BaSO4) has been mentioned in rare cases (Marchev 1991). Sulfur-bearing minerals usually constitute a negligible fraction of the mineral assemblage in magmatic rocks and thus can be classified as accessory minerals. The crystallization of sulfide, sulfate, and S-bearing minerals strongly depends on melt composition, temperature and pressure, and the S speciation in melt which, in turn, is strongly dependent on the prevailing oxygen fugacity (Baker and Moretti 2011, this volume; Wilke et al. 2011, this volume). Irrespectively of the low abundance of S-bearing minerals, the evolution of sulfur in magmas may be evaluated from the occurrence of sulfides and sulfates. These minerals are critical for estimating the activity of various sulfur-bearing species in magmas and can be used to constrain the oxygen fugacity and the S concentration in the melt (e.g., pre-eruptive sulfur concentration in melts). The presence of either sulfide or sulfate in silicate melt indicates that the predominant dissolved sulfur species in the melt are S2− or S6+, respectively. Typically one of these species is dominant, but there are conditions where S2− ...
ISSN: 1529-6466

hal-00644580
https://hal.archives-ouvertes.fr/hal-00644580
DOI : 10.2138/rmg.2011.73.10

Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
Enhancers are developmentally controlled transcriptional regulatory regions whose activities are modulated through histone modifications or histone variant deposition. In this study, we show by genome-wide mapping that the newly discovered deoxyribonucleic acid (DNA) modification 5-hydroxymethylcytosine (5hmC) is dynamically associated with transcription factor binding to distal regulatory sites during neural differentiation of mouse P19 cells and during adipocyte differentiation of mouse 3T3-L1 cells. Functional annotation reveals that regions gaining 5hmC are associated with genes expressed either in neural tissues when P19 cells undergo neural differentiation or in adipose tissue when 3T3-L1 cells undergo adipocyte differentiation. Furthermore, distal regions gaining 5hmC together with H3K4me2 and H3K27ac in P19 cells behave as differentiation-dependent transcriptional enhancers. Identified regions are enriched in motifs for transcription factors regulating specific cell fates such as Meis1 in P19 cells and PPARγ in 3T3-L1 cells. Accordingly, a fraction of hydroxymethylated Meis1 sites were associated with a dynamic engagement of the 5-methylcytosine hydroxylase Tet1. In addition, kinetic studies of cytosine hydroxymethylation of selected enhancers indicated that DNA hydroxymethylation is an early event of enhancer activation. Hence, acquisition of 5hmC in cell-specific distal regulatory regions may represent a major event of enhancer progression toward an active state and participate in selective activation of tissue-specific genes.
ISSN: 0305-1048

inserm-00716879
http://www.hal.inserm.fr/inserm-00716879
http://www.hal.inserm.fr/inserm-00716879/document
http://www.hal.inserm.fr/inserm-00716879/file/nar.gks595.full.pdf
DOI : 10.1093/nar/gks595

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : International audience
The present study is a contribution to the "neXt50 challenge", a coordinated effort across C-HPP teams to identify the SO most tractable missing proteins (MPs) on each chromosome. We report the targeted search of 38 theoretically detectable MPs from chromosomes 2 and 14 in Triton X-100 soluble and insoluble sperm fractions from a total of 15 healthy donors. A targeted mass spectrometry-based strategy consisting of the development of LC-PRM assays (with heavy labeled synthetic peptides) targeting 92 proteotypic peptides of the 38 selected MPs was used. Out of the 38 selected MPs, 12 were identified with two or more peptides and 3 with one peptide after extensive SDS-PAGE fractionation of the two samples and with overall low-intensity signals. The PRM data are available via ProteomeXchange in PASSEL (PASS01013). Further validation by immunohistochemistry on human testes sections and cytochemistry on sperm smears was performed for eight MPs with antibodies available from the Human Protein Atlas. Deep analysis of human sperm still allows the validation of MPs and therefore contributes to the C-HPP worldwide effort. We anticipate that our results will be of interest to the reproductive biology community because an in-depth analysis of these MPs may identify potential new candidates in the context of human idiopathic infertilities.
ISSN: 1535-3893

hal-01681656
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01681656
DOI : 10.1021/acs.jproteome.7b00374