Manioc : Portail | resultats 1 - 20 pour la recherche: Leba, Louis-Jérôme

Annuaire de la vie martiniquaise : 1936
Auteur(s) : Sylvestre, Emile (1...-19.. ; fonctionnaire) Lotaut, Eustache
Auteurs secondaires : Eboué, Félix (1884-1944)
Année de publication :

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Antiplasmodial activities of dyes against Plasmodium falciparum asexual and sexual stages: Contrasted uptakes of triarylmethanes Brilliant green, Green S (E142), and Patent Blue V (E131) by erythrocytes
Auteur(s) : Leba, Louis-Jérôme Popovici, Jean Estevez, Yannick Pelleau, Stéphane Legrand, Eric Musset, Lise DUPLAIS, Christophe
Auteurs secondaires : Laboratoire de Parasitologie, Centre National de Référence du Paludisme - Région Antilles-Guyane ; Institut Pasteur de la Guyane - Réseau International des Instituts Pasteur (RIIP) - WHO Collaborating Center for Surveillance of Antimalarial Drug Resistance Université de Guyane (UG) Institut Pasteur du Cambodge Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS) Génétique et Génomique des Insectes vecteurs ; Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS) This work was supported by European commission (REGPOT-CT-2011-285837-STRonGer), by Institut Pasteur (ACIP A10-2013) and by Investissement d’Avenir of the ANR (CEBA: ANR-10-LABX-25-01) We thank MR4 for providing us Plasmodium falciparum 7G8 (MRA-926 contributed by Thomas Wellems) and 3D7 (MRA-102 contributed by Daniel J. Carucci) malaria parasites. ANR-10-LABX-25-01/10-LABX-0025, CEBA, CEnter of the study of Biodiversity in Amazonia(2010)

Éditeur(s) : HAL CCSD
Résumé : International audience
The search for safe antimalarial compounds acting against asexual symptom-responsible stages and sexual transmission-responsible forms of Plasmodium species is one of the major challenges in malaria elimination programs. So far, among current drugs approved for human use, only primaquine has transmission-blocking activity. The discovery of small molecules targeting different Plasmodium falcip-arum life stages remains a priority in antimalarial drug research. In this context, several independent studies have recently reported antiplasmodial and transmission-blocking activities of commonly used stains, dyes and fluorescent probes against P. falciparum including chloroquine-resistant isolates. Herein we have studied the antimalarial activities of dyes with different scaffold and we report that the triar-ylmethane dye (TRAM) Brilliant green inhibits the growth of asexual stages (IC 50 2 mM) and has exflagellation-blocking activity (IC 50 800 nM) against P. falciparum reference strains (3D7, 7G8) and chloroquine-resistant clinical isolate (Q206). In a second step we have investigated the antiplasmodial activities of two polysulfonated triarylmethane food dyes. Green S (E142) is weakly active against P. falciparum asexual stage (IC 50 x 17 mM) whereas Patent Blue V (E131) is inactive in both antimalarial assays. By applying liquid chromatography techniques for the culture supernatant analysis after cell washings and lysis, we report the detection of Brilliant green in erythrocytes, the selective uptake of Green S (E142) by infected erythrocytes, whereas Patent Blue V (E131) could not be detected within non-infected and 3D7-infected erythrocytes. Overall, our results suggest that two polysulfonated food dyes might display different affinity with transporters or channels on infected RBC membrane.
International journal for parasitology. Drugs and drug resistance

Droits : http://creativecommons.org/licenses/by-nc-sa/
pasteur-01614675
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675/file/Leba%20LJ-article%20colorant-version%20publiee.pdf
DOI : 10.1016/j.ijpddr.2017.07.002

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Histoire maritime de France, comprenant l'histoire des provinces, des villes maritimes, des combats de mer ... par Léon Guérin ... Nouvelle éd. entièrement revue et augmentée de trois volumes...
Auteur(s) : Guérin, Léon (1807-1885?)
Année de publication :

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Use of Plasmodium falciparum culture-adapted field isolates for in vitro exflagellation-blocking assay.
Auteur(s) : Leba, Louis-Jérôme Musset, Lise Pelleau, Stéphane Estevez, Yannick Birer, Caroline Briolant, Sébastien Witkowski, Benoit Ménard, Didier
Auteurs secondaires : Laboratoire de Parasitologie ; Institut Pasteur de la Guyane - Réseau International de Instituts Pasteur Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS) Direction Interarmées du Service de Santé en Guyane ; Direction Interarmées du Service de Santé en Guyane Institut de Recherche Biomédicale des Armées (IRBA) ; Service de Santé des Armées Institut Pasteur du Cambodge ; Institut Pasteur du Cambodge - Réseau International des Instituts Pasteur (RIIP) Department of Life Sciences ; Imperial College London This work was supported by a European commission REGPOT-CT-2011-285837-STRonGer Grant within the FP7, by an Institut Pasteur ACIP (A10-2013) Grant and by Investissement d’Avenir Grant of the ANR (CEBA: ANR-10-LABX-25-01). ANR-10- LABX-25-01, CEBA, Labex CEBA ANR-10- LABX-25-01

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
A major requirement for malaria elimination is the development of transmission-blocking interventions. In vitro transmission-blocking bioassays currently mostly rely on the use of very few Plasmodium falciparum reference laboratory strains isolated decades ago. To fill a piece of the gap between laboratory experimental models and natural systems, the purpose of this work was to determine if culture-adapted field isolates of P. falciparum are suitable for in vitro transmission-blocking bioassays targeting functional maturity of male gametocytes: exflagellation. Plasmodium falciparum isolates were adapted to in vitro culture before being used for in vitro gametocyte production. Maturation was assessed by microscopic observation of gametocyte morphology over time of culture and the functional viability of male gametocytes was assessed by microscopic counting of exflagellating gametocytes. Suitability for in vitro exflagellation-blocking bioassays was determined using dihydroartemisinin and methylene blue. In vitro gametocyte production was achieved using two isolates from French Guiana and two isolates from Cambodia. Functional maturity of male gametocytes was assessed by exflagellation observations and all four isolates could be used in exflagellation-blocking bioassays with adequate response to methylene blue and dihydroartemisinin. This work shows that in vitro culture-adapted P. falciparum field isolates of different genetic background, from South America and Southeast Asia, can successfully be used for bioassays targeting the male gametocyte to gamete transition, exflagellation.
ISSN: 1475-2875

Droits : http://creativecommons.org/licenses/by/
pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01182753/file/LeBas%20et%202015%20version%20publie%20%281%29.pdf
DOI : 10.1186/s12936-015-0752-x

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