Éditeur(s) : HAL CCSD
Résumé : International audience
Science & Sports

hal-01622939
https://hal-umontpellier.archives-ouvertes.fr/hal-01622939
DOI : 10.1016/S0765-1597(02)00065-5

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
ISSN: 1439-6319

hal-01622955
https://hal-umontpellier.archives-ouvertes.fr/hal-01622955
DOI : 10.1007/s00421-002-0637-x

Éditeur(s) : HAL CCSD Thieme Publishing
Résumé : International audience
The aim of the present study was to compare the effects of prior cycling and a successive run on respiratory muscle performance during a cycle-run succession as performed in the triathlon. We hypothesized that despite the moderate intensity of exercise and the absence of exhaustion, the crouched cycling position would induce a decrease in respiratory muscle performance that would be reversed by the successive vertical run position. Ten male triathletes (22.6 +/- 1.1 yr) performed a four-trial protocol: (1) an incremental cycle test to assess maximal oxygen uptake (VO2max), (2) 20 min of cycling (C), (3) 20 min of running (R), and (4) 20 min of cycling followed by 20 min of running (C-R). Trials 2, 3 and 4 were performed at the same metabolic intensity, i. e., 75 % of VO2max. Respiratory muscle force was assessed by measuring maximal expiratory (P(Emax)) and inspiratory (P(Imax)) pressures from the functional residual capacity (FRC) before and 10 min after C, R, and C-R. Respiratory muscle endurance was assessed one day before and 30 min after C, R, and C-R, by measuring the time limit (T(lim)), which corresponds to the length of time a respiratory load can be sustained before the process of fatigue develops sufficiently to cause task failure. The results showed a similar significant decrease in P(Imax) (132.4 +/- 4.9 versus 125.7 +/- 5.6 cm H2O, p < 0.05) and T(lim) (5.22 +/- 0.28 versus 3.68 +/- 0.32 min, p < 0.05) post-C and post-C-R (133.7 +/- 4.0 versus 126.9 +/- 5.2 cm H2O, and 5.29 +/- 0.18 versus 3.49 +/- 0.41 min, respectively, p < 0.05) compared with the pre-trial values. In contrast, P(Imax) and T(lim) were not significantly decreased post-R (131.8 +/- 6.1 cm H2O versus 129.6 +/- 6.4 cm H2O, and 4.90 +/- 0.69 versus 4.40 +/- 0.56 min, respectively, p > 0.05). We concluded that moderate intensity exercise not performed to exhaustion induced a decrease in respiratory muscle performance. Moreover, the respiratory muscle fatigue induced by prior cycling was maintained, and neither reversed nor worsened, by the successive run.
ISSN: 0172-4622

hal-01622938
https://hal-umontpellier.archives-ouvertes.fr/hal-01622938
DOI : 10.1055/s-2003-37201
PUBMED : 12582954

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : International audience
Measurements of branch xylem density, D_x, were made for 1466 trees representing 503 species, sampled from 80 sites across the Amazon basin. Measured values ranged from 240 kg m^?3 for a Brosimum parinarioides from Tapajos in West Pará, Brazil to 1130 kg m^?3 for an Aiouea sp. from Caxiuana, Central Pará, Brazil. Analysis of variance showed significant differences in average D_x across the sample plots as well as significant differences between families, genera and species. A partitioning of the total variance in the dataset showed that geographic location and plot accounted for 33% of the variation with species identity accounting for an additional 27%; the remaining "residual" 40% of the variance accounted for by tree to tree (within species) variation. Variations in plot means, were, however, hardly accountable at all by differences in species composition. Rather, it would seem that variations of xylem density at plot level must be explained by the effects of soils and/or climate. This conclusion is supported by the observation that the xylem density of the more widely distributed species varied systematically from plot to plot. Thus, as well as having a genetic component branch xylem density is a plastic trait that, for any given species, varies according to where the tree is growing and in a predictable manner. Exceptions to this general rule may be some pioneers belonging to Pourouma and Miconia and some species within the genera Brosimum, Rinorea and Trichillia which seem to be more constrained in terms of this plasticity than most species sampled as part of this study.
ISSN: 1810-6277

hal-00298004
https://hal.archives-ouvertes.fr/hal-00298004
https://hal.archives-ouvertes.fr/hal-00298004/document
https://hal.archives-ouvertes.fr/hal-00298004/file/bgd-5-2003-2008.pdf

Éditeur(s) : ISTPM
Résumé : Cette première campagne de la "Thalassa" sur les bancs occidentaux du Groënland pendant le mois de juillet et la première quinzaine d'août 1970 nous a permis de tirer d'intéressantes conclusions sur l'état actuel du stock de morue, la biologie des différentes espèces rencontrées et les conditions de milieu marin. Elle représente en outre une fructueuse expérience en ce qui concerne la technique de chalutage sur ces fonds particulièrement tourmentés, dans des conditions de navigation souvent difficiles. L'extension de la banquise issue de la région orientale du Groënland ne nous a pas permis d'étudier les bancs du premier tiers sud et nos travaux n'ont pu commencer qu'à hauteur du banc Dana. Les observations hydrologiques montrent que pendant l'été 1970 les eaux arctiques de la couche supérieure sont demeurées très froides et que le réchauffement superficiel était à peine marqué à partir du banc Fyllas pour atteindre un maximum de 4°20 sur le banc Disko. Quant à la formation sousjacente, originaire de la Mer d'Irminger, sa température maximale, de 5°, était proche de celle de la moyenne des années précédentes. Ce refroidissement de la couche supérieure, que l'on constatait déjà en 1966 et qui semble avoir atteint son degré le plus bas en 1968 et 1969, n'est pas sans avoir une incidence sur la répartition de la morue - notamment au moment de la ponte - et sur l'appauvrissement du stock. C'est sur le banc Dana seulement que les captures de morue ont atteint un aspect commercial par le bon rendement obtenu et aussi par un fort pourcentage de poissons de taille moyenne. Plus au N, sur le banc Fiskenaes, le rendement a fortement diminué pour devenir très faible sur le banc Fyllas et presque nul sur les bancs Lille et Store Hellefiske. Dans le secteur le plus septentrional, quelques individus seulement ont été pêchés au S du banc Disko. A partir de ce secteur, la prospection a d'ailleurs montré que la détection était nulle et, comme la plupart des fonds étaient impraticables, les chalutages ont été très réduits en nombre et en durée.
Science et Pêche (0036-8350) (ISTPM), 1970-12 , Vol. 198 , P. 1-26

Droits : Ifremer
http://archimer.ifremer.fr/doc/1970/publication-7004.pdf
http://archimer.ifremer.fr/doc/00000/7004/