Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Most tumors undergo metabolic reprogramming towards glycolysis, the so-called Warburg effect, to support growth and survival. Overexpression of IF1, the physiological inhibitor of the F0F1ATPase, has been related to this phenomenon and appears to be a relevant marker in cancer. Environmental contributions to cancer development are now widely accepted but little is known about the underlying intracellular mechanisms. Among the environmental pollutants humans are commonly exposed to, benzo[a]pyrene (B[a]P), the prototype molecule of polycyclic aromatic hydrocarbons (PAHs), is a well-known human carcinogen. Besides apoptotic signals, B[a]P can also induce survival signals in liver cells, both likely involved in cancer promotion. Our previous works showed that B[a]P elicited a Warburg-like effect, thus favoring cell survival. The present study aimed at further elucidating the molecular mechanisms involved in the B[a]P-induced metabolic reprogramming, by testing the possible involvement of IF1. We presently demonstrate, both in vitro and in vivo, that PAHs, especially B[a]P, strongly increase IF1 expression. Such an increase, which might rely on β2-adrenergic receptor activation, notably participates to the B[a]P-induced glycolytic shift and cell survival in liver cells. By identifying IF1 as a target of PAHs, this study provides new insights about how environmental factors may contribute to related carcinogenesis.
ISSN: 2045-2322

hal-01518808
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01518808
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01518808/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01518808/file/Hardonni%C3%A8re%20et%20al%202017.pdf
DOI : 10.1038/s41598-017-00269-7

Éditeur(s) : HAL CCSD Elsevier Elsevier Masson
Résumé : International audience
La maladie alcoolique du foie se caractérise par une stéatose précoce, qui, pour seulement 20 % des buveurs excessifs, peut évoluer vers la stéatohépatite ou la cirrhose. À l'heure actuelle, la description des facteurs de risque et des mécanismes cellulaires et moléculaires impliqués dans la progression de la maladie restent toujours incomplète. Ces dernières années, les radeaux lipidiques, microstructures de la membrane plasmique, ont été décrits comme une nouvelle cible de l'exposition à l'éthanol. Or les acides gras polyinsaturés oméga 3, présents naturellement dans certains aliments comme les poissons gras, se sont révélés être capables de modifier le microenvironnement physicochimique de ces microstructures avec des effets bénéfiques pour certaines pathologies comme l'inflammation ou le cancer. L'objectif de cette revue est ainsi d'établir un lien entre les altérations membranaires induites par les oméga 3 et leurs effets sur les mécanismes moléculaires impliqués dans la progression de la maladie alcoolique du foie.
ISSN: 0985-0562

hal-00966797
https://hal.archives-ouvertes.fr/hal-00966797
https://hal.archives-ouvertes.fr/hal-00966797/document
https://hal.archives-ouvertes.fr/hal-00966797/file/Acides_gras_polyinsaturA_s_omA_ga_3_et_toxicitA_hA_patique_de_la_A_thanol_rA_le_du_remodelage_membranaire.pdf
DOI : 10.1016/j.nupar.2013.10.003

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
: Previously, we demonstrated that eicosapentaenoic acid enhanced ethanol-induced oxidative stress and cell death in primary rat hepatocytes via an increase in membrane fluidity and lipid raft clustering. In this context, another n-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA), was tested with a special emphasis on physical and chemical alteration of lipid rafts. Pretreatment of hepatocytes with DHA reduced significantly ethanol-induced oxidative stress and cell death. DHA protection could be related to an alteration of lipid rafts. Indeed, rafts exhibited a marked increase in membrane fluidity and packing defects leading to the exclusion of a raft protein marker, flotillin. Furthermore, DHA strongly inhibited disulfide bridge formation, even in control cells, thus suggesting a disruption of protein-protein interactions inside lipid rafts. This particular spatial organization of lipid rafts due to DHA subsequently prevented the ethanol-induced lipid raft clustering. Such a prevention was then responsible for the inhibition of phospholipase C-γ translocation into rafts, and consequently of both lysosome accumulation and elevation in cellular low-molecular-weight iron content, a prooxidant factor. In total, the present study suggests that DHA supplementation could represent a new preventive approach for patients with alcoholic liver disease based upon modulation of the membrane structures.
ISSN: 0278-6915

hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/file/Sergent-et-al-incl-figures-accepted.pdf
DOI : 10.1016/j.fct.2013.07.061

Éditeur(s) : HAL CCSD
Résumé : Session 3: Health & Nutrition/Fat and Salt Reduction
absent
The eighth Cheese symposium
Moorepark, Ireland

hal-01409434
https://hal.archives-ouvertes.fr/hal-01409434
PRODINRA : 47217

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Human leishmaniasis are widely spread diseases with different clinical forms caused by about 20 species within the Leishmania genus. Leishmania species identification is relevant for therapeutic management and prognosis, especially for cutaneous and muco-cutaneous forms. Several methods are available to identify Leishmania species from culture, but they have not been standardized for the majority of the currently described species, with the exception of MultiLocus Enzyme Electrophoresis. Moreover, these techniques are expensive, time-consuming and not available in all laboratories. Within the last decade, mass spectrometry (MS) has been adapted for microorganism identification, including Leishmania However, no commercial reference mass spectral database is available. In this study, a reference mass spectral library (MSL) for Leishmania isolates, accessible through a free web-based application (mass spectral identification, MSI), was constructed and tested. It includes mass spectral data for 33 different Leishmania species, including species that infect humans, animals and phlebotomine vectors. Four laboratories on two continents evaluated MSI performance using 268 samples of which 231 were Leishmania strains. All Leishmania strains, but one, were correctly identified at least at the complex level. A risk of species misidentification within the L. donovani, L. guyanensis and L. braziliensis complexes was observed, as already reported with other techniques. The tested application was reliable, with identification results comparable to those obtained with reference methods, but with a more favorable cost-efficiency ratio. This free online identification system relies on a scalable database and can be implemented directly in the user's computers.
ISSN: 0095-1137

hal-01571305
https://hal.archives-ouvertes.fr/hal-01571305
DOI : 10.1128/JCM.00845-17
PUBMED : 28724559

Éditeur(s) : HAL CCSD
Résumé : International audience
International Scientific Conference on Probiotics and Prebiotics (IPC)
Kosice, Slovakia

hal-01122606
https://hal.archives-ouvertes.fr/hal-01122606

Éditeur(s) : HAL CCSD Centre de Biotechnologies de Bretagne
Résumé :  
absent
4th International Symposium Health Molecules & Ingredients : Proceedings, conferences and posters abstracts
Quimper, France

hal-01409415
https://hal.archives-ouvertes.fr/hal-01409415
PRODINRA : 40774