Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 1433-8319

hal-01095295
https://hal-agroparistech.archives-ouvertes.fr/hal-01095295
DOI : 10.1007/s00606-010-0312-3

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Under physiological conditions, the liver sinusoidal endothelial cells (LSECs) mediate hepatic immune tolerance toward self or foreign antigens through constitutive expression of anti-inflammatory mediators. However, upon viral infection or Toll-like receptor 2 (TLR2) activation, LSECs can achieve proinflammatory functions, but their role in hepatic inflammation during acute viral hepatitis is unknown. Using the highly virulent mouse hepatitis virus type 3 (MHV3) and the attenuated variants 51.6-MHV3 and YAC-MHV3, exhibiting lower tropism for LSECs, we investigated in vivo and in vitro the consequence of LSEC infection on their proinflammatory profiles and the aggravation of acute hepatitis process. In vivo infection with virulent MHV3, in comparison to attenuated strains, resulted in fulminant hepatitis associated with higher hepatic viral load, tissue necrosis, and levels of inflammatory mediators and earlier recruitment of inflammatory cells. Such hepatic inflammatory disorders correlated with disturbed production of interleukin-10 (IL-10) and vascular factors by LSECs. We next showed in vitro that infection of LSECs by the virulent MHV3 strain altered their production of anti-inflammatory cytokines and promoted higher release of proinflammatory and procoagulant factors and earlier cell damage than infection by attenuated strains. This higher replication and proinflammatory activation in LSECs by the virulent MHV3 strain was associated with a specific activation of TLR2 signaling by the virus. We provide evidence that TLR2 activation of LSCEs by MHV3 is an aggravating factor of hepatic inflammation and correlates with the severity of hepatitis. Taken together, these results indicate that preservation of the immunotolerant properties of LSECs during acute viral hepatitis is imperative in order to limit hepatic inflammation and damage. IMPORTANCE Viral hepatitis B and C infections are serious health problems affecting over 350 million and 170 million people worldwide, respectively. It has been suggested that a balance between protection and liver damage mediated by the host's immune response during the acute phase of infection would be determinant in hepatitis outcome. Thus, it appears crucial to identify the factors that predispose in exacerbating liver inflammation to limit hepatocyte injury. Liver sinusoidal endothelial cells (LSECs) can express both anti- and proinflammatory functions, but their role in acute viral hepatitis has never been investigated. Using mouse hepatitis virus (MHV) infections as animal models of viral hepatitis, we report for the first time that in vitro and in vivo infection of LSECs by the pathogenic MHV3 serotype leads to a reversion of their intrinsic anti-inflammatory phenotype toward a proinflammatory profile as well to as disorders in vascular factors, correlating with the severity of hepatitis. These results highlight a new virus-promoted mechanism of exacerbation of liver inflammatory response during acute hepatitis.
ISSN: 0022-538X

hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/file/Mouse%20Hepatitis%20Virus%20Infection_accepted.pdf
DOI : 10.1128/JVI.01069-16

Éditeur(s) : HAL CCSD International Aroid Society
Résumé : International audience
ISSN: 0197-4033

hal-01095862
https://hal-agroparistech.archives-ouvertes.fr/hal-01095862

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Due to their geographic location and traditional diet, rich in seafood and marine mammals, the Inuit living in Arctic Quebec are exposed to high amounts of pollutants, including organochlorine pesticides (OCPs). While the adverse developmental effects of these pesticides on child cognitive functions are well known, the effects of developmental exposure to OP on sensory processes have not been investigated. The aim of this longitudinal study was to assess the effects of prenatal and childhood exposure to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (p,p'-DDT) and its major metabolite 1,1,-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE), on visual processing in Inuit children in Nunavik (Arctic Québec). p,p'-DDT and p,p'-DDE concentrations were determined from umbilical cord and 5- and 11-year plasma samples. Visual evoked potentials (VEPs) were successfully recorded in 150 children at 4 contrast levels (95%, 30%, 12%, and 4%). Hierarchical multiple regressions were conducted to determine the association between p,p'-DDT, or p,p'-DDE, exposure and VEPs while controlling for the effects of various confounders, including fish nutrients and other contaminants. p,p'-DDE measured in umbilical cord plasma was significantly related to the amplitude of the N150 response at the lowest contrast (4%). In addition, 5-year p,p'-DDE plasma concentration was significantly associated with decreased N75 amplitude. These findings indicate that p,p'-DDE exposure, both pre- and postnatally, during early childhood is associated with visual processing impairment later in life.
ISSN: 0161-813X

hal-01061309
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061309
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061309/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061309/file/Prenatal_and_5-year-NEUTOX-D-13-00192R2_v4.pdf
DOI : 10.1016/j.neuro.2014.04.009

Éditeur(s) : HAL CCSD John Libbey Eurotext
Résumé : National audience
ISSN: 1635-0421

hal-01118600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118600
DOI : 10.1684/ers.2014.0751

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Background: Blood Lead Levels (BLLs) have substantially decreased in recent decades in children in France. However, further reducing exposure is a public health goal because there is no clear toxicological threshold. The identification of the environmental determinants of BLLs as well as risk factors associated with high BLLs is important to update prevention strategies. We aimed to estimate the contribution of environmental sources of lead to different BLLs in children in France.Methods: We enrolled 484 children aged from 6 months to 6 years, in a nationwide cross-sectional survey in 2008-2009. We measured lead concentrations in blood and environmental samples (water, soils, household settled dusts, paints, cosmetics and traditional cookware). We performed two models: a multivariate generalized additive model on the geometric mean (GM), and a quantile regression model on the 10th, 25th, 50th, 75th and 90th quantile of BLLs. Results: The GM of BLLs was 13.8 μg/L (=1.38 µg/dL) (95% Confidence Intervals (CI): 12.7-14.9) and the 90th quantile was 25.7 μg/L (CI: 24.2-29.5). Household and common area dust, tap water, interior paint, ceramic cookware, traditional cosmetics, playground soil and dust, and environmental tobacco smoke were associated with the GM of BLLs. Household dust and tap water made the largest contributions to both the GM and the 90th quantile of BLLs. The concentration of lead in dust was positively correlated with all quantiles of BLLs even at low concentrations. Lead concentrations in tap water above 5 µg/L were also positively correlated with the GM, 75th and 90th quantiles of BLLs in children drinking tap water.Conclusions: Preventative actions must target household settled dust and tap water to reduce the BLLs of children in France. The use of traditional cosmetics should be avoided whereas ceramic cookware should be limited to decorative purposes.
ISSN: 0160-4120

Droits : info:eu-repo/semantics/OpenAccess
hal-01099719
https://hal.archives-ouvertes.fr/hal-01099719
https://hal.archives-ouvertes.fr/hal-01099719/document
https://hal.archives-ouvertes.fr/hal-01099719/file/Manuscript_Etchevers_EI_V3_no%20%20changes%20marked.pdf
DOI : 10.1016/j.envint.2014.10.007

Éditeur(s) : HAL CCSD International Aroid Society
Résumé : International audience
ISSN: 0197-4033

hal-01094213
https://hal-agroparistech.archives-ouvertes.fr/hal-01094213

Éditeur(s) : HAL CCSD
Résumé : National audience
Nous définissons l’efficacité des mouvements de tracé comme la capacité à concilier des caractéristiques de rapidité, fluidité et régularité satisfaisant aux attentes des enseignements scolaires. L’équipe éducative en charge, dès l’entrée à l’école maternelle, de l’accompagnement de l’acquisition de l’écriture, ne dispose ni de moyens d’évaluation objectifs et fiables de l’efficacité des mouvements de tracé adaptés aux profils des très jeunes enfants, ni d’outils d’aide à la décision des exercices les plus appropriés pour renforcer et contrôler la progression de chaque enfant compte-tenu de son déjà-là psychomoteur notamment. Nous expliciterons en quoi les dispositifs interactifs d’entrainement graphomoteur orientés stylo sont inadaptés à constituer des aides à la décision et à l’accompagnement des acquisitions pour ces trois domaines. Nous vérifierons ensuite expérimentalement l’existence de pistes, à la fois d’un point de vue technologique et méthodologique, pour doter ces dispositifs de capacité d’évaluation de l’efficacité des mouvements de tracé du jeune apprenti-scripteur.
Atelier IHM'A, actes IHM'14, 26e conférence francophone sur l'Interaction Homme-Machine
Lille, France

Droits : info:eu-repo/semantics/OpenAccess
hal-01089622
https://hal.archives-ouvertes.fr/hal-01089622
https://hal.archives-ouvertes.fr/hal-01089622/document
https://hal.archives-ouvertes.fr/hal-01089622/file/p29-duval.pdf

Éditeur(s) : HAL CCSD
Résumé : International audience
Les auteurs ont mesuré l'augmentation de température dans les spadices de deux espèces de Monstera: Monstera adansonii Schott et Monstera deliciosa Liebm. La température des spadices a été enregistrée parallèlement à la description du cycle floral (mouvement de la spathe, production d'odeur, libération de pollen et de résine) sur des plantes cultivées au Jardin botanique de Montréal. Les cycles floraux et thermogénétiques, qui sont identiques pour les deux espèces, durent 96 heures et s'étendent sur près de cinq jours. Quand la floraison débute (premier jour, phase femelle), la température du spadice augmente légèrement au dessus de la température ambiante jusqu'au début de la phase mâle. Le matin du cinquième jour, quand la température ambiante est à son plus bas, la température du spadice commence à augmenter et atteint un maximum en même temps que le pollen est libéré. Le type de cycles floraux et thermogénétiques indique que M adansonii et M deliciosia pourraient être pollinisés par des insectes (probablement des coléoptères) arrivant avant que la spathe soit complètement déroulée et repartant au moment de la libération du pollen.
Bulletin de la société d'histoire naturelle de Toulouse

hal-01086661
https://hal-agroparistech.archives-ouvertes.fr/hal-01086661

Éditeur(s) : HAL CCSD
Résumé : International audience
The pollination of Philodendron solimoesense (subgenus Meconostigma) was studied in four populations of French Guiana. Flowering is asynchronous within each population during July, and the flowering cycle is a 2-d process. Numerous insects visit Philodendron inflorescences, but the main pollinator seems to be Cyclocephala colasi (Scarabaeidae, Dynastinae). The pollination process displays aspects typical of beetle pollination: the production of heat and of a strong odor, the presence of a food reward (stigmatic secretion and sterile male flowers), and the presence of a copulation chamber. Flower heat production is important (ca. 117C above the ambient air) and may help to volatilize the fragrance. Attraction and choice-test experiments showed that C. colasi is not likely to depend on chemical information (such as pheromone) to localize conspecifics but may rely instead on stimuli produced by the inflorescences in order to meet mating partners
International Journal of Plant Sciences

hal-01202475
https://hal.archives-ouvertes.fr/hal-01202475

Éditeur(s) : HAL CCSD
Résumé : International audience
Viral replication in the liver is generally detected by cellular endosomal Toll-like receptors (TLRs) and cytosolic helicase sensors that trigger antiviral inflammatory responses. Recent evidence suggests that surface TLR2 may also contribute to viral detection through recognition of viral coat proteins but its role in the outcome of acute viral infection remains elusive. In this study, we examined in vivo the role of TLR2 in acute infections induced by the highly hepatotrophic mouse hepatitis virus (MHV) type 3 and weakly hepatotrophic MHV-A59 serotype. To address this, C57BL/6 (wild-type; WT) and TLR2 knockout (KO) groups of mice were intraperitoneally infected with MHV3 or MHV-A59. MHV3 infection provoked a fulminant hepatitis in WT mice, characterized by early mortality and high alanine and aspartate transaminase levels, histopathological lesions and viral replication whereas infection of TLR2 KO mice was markedly less severe. MHV-A59 provoked a comparable mild and subclinical hepatitis in WT and TLR2 KO mice. MHV3-induced fulminant hepatitis in WT mice correlated with higher hepatic expression of interferon-β, interleukin-6, tumour necrosis factor-α, CXCL1, CCL2, CXCL10 and alarmin (interleukin-33) than in MHV-A59-infected WT mice and in MHV3-infected TLR2 KO mice. Intrahepatic recruited neutrophils, natural killer cells, natural killer T cells or macrophages rapidly decreased in MHV3-infected WT mice whereas they were sustained in MHV-A59-infected WT mice and MHV3-infected TLR2 KO. MHV3 in vitro infection of macrophagic cells induced rapid and higher viral replication and/or interleukin-6 induction in comparison to MHV-A59, and depended on viral activation of TLR2 and p38 mitogen-activated protein kinase. Taken together, these results support a new aggravating inflammatory role for TLR2 in MHV3-induced acute fulminant hepatitis. © 2016 John Wiley & Sons Ltd
Immunology

Droits : info:eu-repo/semantics/OpenAccess
hal-01373946
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373946
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373946/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373946/file/Toll-like%20receptor%20%28tlr%29-2%20exacerbates%20murine%20acute%20viral%20hepatitis.pdf
DOI : 10.1111/imm.12627

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Northwest Iran is characterized by a high level of historical and instrumental seismicity related to the ongoing convergence between the Arabian and Eurasian plates. In this region, the main right-lateral strike-slip fault known as the North Tabriz fault (NTF) forms the central portion of a large crustal fault system called the Tabriz fault system (TFS). The NTF is a major seismic source along which at least three strong and destructive earthquakes have occurred since 858 AD. The two most recent destructive seismic events occurred in 1721 AD and 1780 AD, rupturing the SE and NW fault segments, respectively. This paper reports paleoseismological and quantitative geomorphologic investigations on the SE segment of the NTF, between the cities of Bostanabad and Tabriz. These observations help to improve our understanding of the seismic hazard for Tabriz city and its surrounding areas. Our field investigations revealed evidence of successive faulting events since the Late Quaternary. Paleoseismic investigations indicate that since 33.5 kyr, the SE segment of the NTF has experienced at least three major (M>7.5) seismic events, including the 1721 AD earthquake (M=7.6–7.7). Along the NW segment of the fault, however, our results suggest that the amount of strong (M~7.5) seismic events during the same period is significantly greater than along the SE segment. One possible explanation of such a difference in seismic activity is that the Late Quaternary-Holocene coseismic slip rate is decreasing along the NTF from the northwest to the southeast. This explanation contradicts the former hypothesis of a constant slip rate along the whole length of the NTF. In addition, more distributed deformation along several parallel fault branches, in a wider fault zone of the SE segment of the NTF may be considered as additional evidence for the estimation of lower rate of deformation along the fault segment. Such a slip distribution pattern can explain the existence of smaller (~300 m) Pliocene-Quaternary cumulative dextral offsets along the SE fault segment than the measured cumulative offsets along the NW segment (~800 m) of the NTF.
ISSN: 0040-1951

hal-01089420
http://hal.upmc.fr/hal-01089420
http://hal.upmc.fr/hal-01089420/document
http://hal.upmc.fr/hal-01089420/file/Solaymani%20Azad%20et%20al.%2C%202014%20%28accepted%29.pdf
DOI : 10.1016/j.tecto.2014.11.010

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490