Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Up to now, several studies have investigated estrogen receptor (ER)–estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER–ERE interactions.
ISSN: 1618-2642

hal-01418341
https://hal.archives-ouvertes.fr/hal-01418341
https://hal.archives-ouvertes.fr/hal-01418341/document
https://hal.archives-ouvertes.fr/hal-01418341/file/Estrogen%20Receptor%20preparation%20effects_accepted.pdf
DOI : 10.1007/s00216-016-9967-0

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Maghrebides, Betics and some parts of the Calabrian, NE Sicilian and Tuscan allochtons constitute dismembered fragments of the Alpine belt that resulted from the Cenozoic collision between Africa and Eurasia and the opening of the Western Mediterranean basin. Mineral and whole-rock geochemical analyses have been performed on three distinct outcrops of mantle material from the Edough Massif of NE Algeria, namely the Bou Maiza (BM) gabbros, the La Voile Noire (LVN) amphibolites and the Sidi Mohamed (SM) peridotites. In all samples, Sr isotopes are largely affected by seawater alteration (87Sr/86Sract. > 0.70384 and up to 0.70888) and cannot be used to evaluate the nature of the source reservoirs. SM peridotites display variable depleted mantle Nd isotopic signatures (εNdact. from + 7.0 to + 12.2) and geochemical features suggesting no significant chemical depletion as a result of partial melting and melt extraction (Mg# < 90; slightly LREE-depleted patterns with La/YbN = 0.33-0.39). These rocks are interpreted as parts of the subcontinental lithospheric mantle incorporated into the crustal units of the Edough Massif during the early stages of opening of the Algerian basin. BM gabbros and LVN amphibolites show geochemical signatures indicating derivation from a common Depleted Mantle reservoir (εNd > + 7.9) and are likely cogenetic, but without filiation with the SM peridotites. Pb isotopes indicate a contribution of sediments in the source reservoir, which is attributed to contamination solely by hydrous fluids released from a sedimentary component. This observation, together with a LILE-enrichment, suggests a back-arc basin environment. These results indicate that BM and LVN units were obducted onto the North African margin and subsequently fragmented, probably during doming and exhumation of the lower continental crust of the Edough massif. Doming resulted in opposite movements of the overlying oceanic units, southward for the BM units and northward for LVN amphibolites.
ISSN: 0040-1951

hal-01122777
https://hal.archives-ouvertes.fr/hal-01122777
DOI : 10.1016/j.tecto.2014.03.031

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Nonylphenol (NP) is an endocrine disruptor with harmful effects including feminization and carcinogenesis on various organisms. This substance is a degradation product of nonylphenol ethoxylates (NPEO) that is used in several industrial and agricultural processes. In this paper, we examined the assessment of NP exposure on chick embryo development, using a concentration consistent with the environmental concentrations of NP. With this aim, NP (between 0.1 and 50μg/egg) was injected into the yolk of egg through a small needle hole in the shell. We report the effect of NP on chick reproductive system development although the effect we observed is lower than those observed by exposition to other endocrine disruptors. However, histological analysis highlighted a decrease of intraluminal seminiferous surface area in 64.12% of case (P=0.0086) and an heterogeneous organization of the renal tubules when 10μg/egg were injected. Moreover, an impairment of liver development with an abnormal bile spillage was observed when higher concentration of NP was injected (50μg/egg).
Environment International

hal-00878651
https://hal.archives-ouvertes.fr/hal-00878651
https://hal.archives-ouvertes.fr/hal-00878651/document

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This study presents new geochemical data on 26 mafic lavas from the Middle Atlas and Central Morocco volcanic provinces, including Miocene nephelinites and Pliocene-Quaternary (3.9-0.6Ma) nephelinites, basanites, alkali and subalkaline basalts. Most of them represent near-primary magmas, although some alkali basalts were derived from the minor fractionation of olivine and diopside phenocrysts. These evolved samples and the subalkaline basalt display higher 207Pb/204Pb and Zr/Nb ratios and lower εNd consistent with their contamination by lower crustal granulites during an open fractionation process. The progressive enrichment in incompatible elements observed from alkali basalts to nephelinites suggests their derivation from decreasing partial melting degrees of an enriched mantle source located at the garnet-spinel transition zone. The strong negative spikes observed for K in multielement patterns indicate that this source contained a residual pargasitic amphibole. We propose that partial melting occurred at around 2GPa, i.e. near the lithosphere-asthenosphere boundary beneath the Middle Atlas (60-80km).The trace element and isotopic Sr-Nd-Pb-Hf signature of the uncontaminated lavas displays a geochemical flavour intermediate between those of high μ (HIMU), "C", and enriched mantle components. It is very similar to that of abundant metasomatic amphibole- and clinopyroxene-rich lithospheric peridotites and pyroxenites carried by Middle Atlas lavas, which likely represent an analog of the source of these lavas. It is therefore not necessary to postulate the contribution of a "fresh" asthenospheric mantle to their genesis. We propose that they resulted from the partial melting of the base of a veined lithospheric mantle metasomatised during the late Cretaceous by alkaline melts from the Central Atlantic plume, the ancestor of the Canary plume. Melting was probably triggered by the flux of a hot mantle within a regional SW-NE sub-lithospheric channel, in response to either the Alboran slab retreat or edge-driven convection around the West African craton.
ISSN: 0024-4937

insu-01056523
https://hal-insu.archives-ouvertes.fr/insu-01056523
DOI : 10.1016/j.lithos.2014.07.009

Éditeur(s) : HAL CCSD
Résumé : International audience
Environmental context The safeguarding of water supplies and drinking water is a major issue when considering human health risk management. In this context, an automatic and on-site fractionation system for the detection of organic contaminants has been developed. The main goal of this system is to establish an initial diagnosis by identifying a class of substances involved in a case of pollution. Abstract This paper proposes a new approach for the preconcentration, fractionation, prediction and detection of organic micropollutants in water. The main aim of this study was to implement an innovative fractionation method and a prediction model based on the physicochemical properties of compounds and interactions with the sorbent of solid-phase extraction cartridges. Two sorbents (Strata-SAX and Oasis-HLB) and three specific eluting solvents (mixture of methanol and sodium chloride and mixtures of acetonitrile and ultrapure water) were used to separate organic compounds into five specific fractions according to their physicochemical properties (anionic and cationic or neutral compounds with various polarity). More than 75 molecules (43 molecules individually studied and a mixture with 43 molecules, including 11 molecules individually studied) including pesticides, pharmaceuticals, endocrine disruptors and polycyclic aromatic hydrocarbons, with various properties were studied, and the results showed that the elution fraction can be predicted for more than 85% of the compounds. This methodology could simplify the analytical chain by reducing detailed analysis on limited categories of compounds, and could be used for a rapid and on-site screening of organic compounds. © CSIRO 2016.
Environmental Chemistry

hal-01373945
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373945
DOI : 10.1071/EN15135

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The estrogen receptor (ER) is a transcription factor that binds under 17-b-estradiol (E2) stimulation as homodimer to a short DNA consensus sequence named estrogen response element (ERE). The ER/ERE interaction has been assessed by several research groups through different methodologies notably by surface plasmon resonance (SPR) techniques. The biochemical parameters and conditions (solvent, ER concentration, salt, time and temperature) used to prepare samples before analysis were very different from one study to another. But no studies have aimed to compare the effect of these modifications on ER/ERE interaction. Therefore the main objective of the present paper was to assess the influence of biochemical parameters onto the ER/ERE interaction with the final aim to improve the comprehension of this interaction. Our results highlighted that parameters like solvent, ER concentration, salt and surfactant concentration, temperature and time deeply modify ER/ERE interaction. Nevertheless, the dimer formation under E2 stimulation occurred with all tested conditions. Altogether, incubation parameters of ER with E2, deeply modify its binding level onto ERE. These data constitute an important key point to consider for the improvement of ER/ERE detection method depending upon the aim of the study (interaction measurement, environmental detection, development of new technologies or devices).
ISSN: 0003-9861

hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061/document
https://hal.archives-ouvertes.fr/hal-00936061/file/Impactsur biochemical_design_on_estrogen_receptor_estrogen_response_element_interaction_by_surface_plasmon_resonance_technology-accepted.pdf
DOI : 10.1016/j.abb.2013.11.006