Éditeur(s) : HAL CCSD
Résumé : International audience
Two fluorescent galactofuranosides were synthesized and their biological activities evaluated on non-infected and Leishmania infected macrophages. Both tagged scaffolds were able to penetrate macrophages. Compared to the activity of the parent octyl galactofuranoside used as a reference, the fluorescein-conjugate showed altered biological properties while the rhodamine 6G one synergistically acted with the lipid chain to significantly increase antiparasitic activity.
Bioorganic & Medicinal Chemistry Letters

hal-01446721
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446721
PUBMED : 27956346
DOI : 10.1016/j.bmcl.2016.11.090

Éditeur(s) : Université Paris 6
Résumé : The aggregative behaviour of the dolphinfish (Coryphaena hippurus) is studied in order to improve our knowledge of the mechanism involved in the association of this species with the floating objects and to evaluate its potential impact in term of exploitation, targeted or not. This PhD thesis is composed of four chapters. Through an extensive analyze of the literature, the first chapter takes stock of the present knowledge on the FADs, on the aggregative behaviour of pelagic fish and on the dolphinfish. The second chapter deals specifically with the aggregative behaviour of the dolphinfish by the way of acoustic tagging of fish carry out during offshore campaigns around drifting FADs. The feeding habits of the dolphinfish around FADs are the main purpose of the third chapter. It is studied by the way of two complementary methods : stomach contains analysis of dolphinfish aggregated around several types of floating objects and underwater inventory of the marine fauna under these floating objects. Finally, the fourth and last chapter sums all the results and suggests a theory to explain the aggregative behaviour of the dolphinfish. We also try to estimate the possible consequences of this behaviour on the abundance and on the distribution of this species on a larger scale.
Le comportement agrégatif de la dorade coryphène (Coryphaena hippurus) est étudié dans le but de mieux comprendre le déterminisme de l'association de cette espèce avec les objets flottants et d'en évaluer les impacts potentiels en terme de sensibilité à l'exploitation halieutique, qu'elle soit ciblée ou non. Ce mémoire de thèse est organisé en quatre chapitres. A travers une analyse approfondie de la bibliographie, le premier chapitre présente un bilan des connaissances actuelles sur les DCP, l'agrégation des poissons pélagiques et la dorade coryphène. Le second chapitre traite plus spécifiquement du comportement agrégatif de la dorade coryphène. Il a été abordé à l'aide de marquages électroniques réalisés au cours de campagnes hauturières autour de DCP dérivants. Le comportement trophique de la dorade coryphène fait l'objet du troisième chapitre. Il est abordé par l'utilisation conjointe de deux méthodes d'investigation : l'analyse des contenus stomacaux de dorades coryphènes agrégées sous divers types d'objets flottants et la réalisation d'inventaires faunistiques sous-marins autour de ces objets flottants. Enfin, le quatrième et dernier chapitre synthétise l'ensemble des résultats obtenus, propose une théorie du comportement agrégatif de la dorade coryphène et tente d'évaluer les conséquences possibles de ce comportement sur la distribution et l'abondance de l'espèce à grande échelle.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2004/these-323.pdf
http://archimer.ifremer.fr/doc/00000/323/

Éditeur(s) : Editions de l'ORSTOM
Résumé : A research programm on the marine green turtle Chelonia mydas nesting on the two major reproduction sites for the species in the Indian Ocean, conducled over last 15 years, included intensive tagging programm. Processing of these lagging data provides estimations of adult female populations {or each site and season from two independent methods. First classical method is the ratio of total number of nests produced over a define time on the mean individual number of nests by female during the same time (November, December, January). The computed mean individual number ranges from 3 to 3.5 obtained over 2 to 3 months. The second method is the multiple capture-recapture method (JOLLY, 1965; SEBER, 1965; MANLY, 1984). Il is an original tentative of applying this method for the daily estimation of females number on a nesting site. The results obtained suggest this method to be applied for the species on other sites and to other marine turtle species. Population estimates obtained by these two independent methods are coherent Roughly, the two populations through seasonal nesting activity appear in wealthy state with a mean estimation number of 850 to 1100 females for Tromelin and more variable eslimations ranging from 2000 lo 11 000 females by season for Europa, considering lhe three major nesting months: November to January
Un programme d'observations sur les populations de tortue verte marine Chelonia mydas pondant sur les deux sites de ponte majeurs de l'espèce dans l'océan Indien, Tromelin et Europa, comportait notamment un programme intensif de marquage. L'exploitation de ces données de marquage a permis de réaliser des estimations du nombre de femelles pondant sur chaque site par deux méthodes différentes. La première méthode est celle, classiquement utilisée, du rapport entre le nombre total de pontes déposées pour une période donnée et le nombre moyen de pontes par femelle durant le même temps. On démontre que le nombre moyen individuel de ponte est de l'ordre de 3 à 3,5 pontes par saison. La deuxième méthode est celle des estimations par marquages-recaptures multiples (J OLL Y -SEBER, 1965; MANLY, 1984). C'est une utilisation originale de cette méthode pour l'estimation journalière des populations de tortues femelles sur un site de ponte, qui doit pouvoir être appliquée sur d'autres sites pour la même espèce et pour d'autres espèces de tortues marines. Les résultats obtenus par ces deux méthodes différentes sont cohérents. Schématiquement, on peut conclure à deux populations abondantes de 850 à 1 ZOO femelles sur Tromelin par saison et des estimations moyennes variant de 2 000 à Il 000 tortues par saison pour l'ensemble des plages d'Europa pour les trois mois principaux de ponte par saison: novembre à janvier.
Océanographie tropicale (0245-9418) (Editions de l'ORSTOM), 1986 , Vol. 21 , N. 1 , P. 3-22

Droits : ORSTOM
http://archimer.ifremer.fr/doc/00113/22406/20103.pdf
http://archimer.ifremer.fr/doc/00113/22406/

Éditeur(s) : Gauthier-Villars
Résumé : Two algal communities on unconsolidated sand, dominated by two Caulerpa populations, were studied in the southwestern New Caledonian lagoon. Biomass, growth rates and production were measured at two stations over one year. Biomass and growth rate of Halimeda were estimated monthly by counting the number of new and lost segments on tagged plants and by weighing them. Production was assessed monthly by comparing two successive measurements. The average rates measured were 0.60 s f-1 d-1 (segment frond-1 day-1 ) for Halimeda incrassata and 0. 15 s f-1 d-1 for H. discoidea. Production in terms of organic carbon amounted to 3.79 g m-2 y-1 for H. incrassata and 3.37 g m-2 y-1 for H. discoidea. The production of H. discoidea population is subject to seasonal variations.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1991 , Vol. 14 , N. 6 , P. 581-588

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00102/21277/18886.pdf
http://archimer.ifremer.fr/doc/00102/21277/

Éditeur(s) : HAL CCSD The Royal Society
Résumé : International audience
Conservation commonly requires trade-offs between social and ecological goals. For tropical small-scale fisheries, spatial scales of socially appropriate management are generally small— the median no-take locally managed marine area (LMMA) area throughout the Pacific is less than 1 km 2. This is of particular concern for large coral reef fishes, such as many species of grouper, which migrate to aggregations to spawn. Current data suggest that the catchment areas (i.e. total area from which individuals are drawn) of such aggregations are at spatial scales that preclude effective community-based management with no-take LMMAs. We used acoustic telemetry and tag-returns to examine reproductive migrations and catchment areas of the grouper Epinephelus fuscoguttatus at a spawning aggregation in Papua New Guinea. Protection of the resultant catchment area of approximately 16 km 2 using a no-take LMMA is socially untenable here and throughout much of the Pacific region. However, we found that spawning migrations were skewed towards shorter distances. Consequently, expanding the current 0.2 km 2 no-take LMMA to 1–2 km 2 would protect approximately 30–50% of the spawning population throughout the non-spawning season. Contrasting with current knowledge, our results demonstrate that species with moderate reproductive migrations can be managed at scales congruous with spatially restricted management tools.
ISSN: 2054-5703

Droits : http://creativecommons.org/licenses/by/
hal-01297717
https://hal-univ-perp.archives-ouvertes.fr/hal-01297717
https://hal-univ-perp.archives-ouvertes.fr/hal-01297717/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01297717/file/150694.full.pdf
DOI : 10.1098/rsos.150694

Éditeur(s) : HAL CCSD
Résumé : International audience
Wound healing is important for sharks from the earliest life stages, for example, as the 'umbilical scar' in viviparous species heals, and throughout adulthood, when sharks can incur a range of external injuries from natural and anthropogenic sources. Despite anecdotal accounts of rapid healing in elasmobranchs, data regarding recovery and survival of individuals from different wound or injury types has not been systematically collected. The present study documented: (i) 'umbilical scar' healing in wild-caught, neonatal blacktip reef sharks while being reared for 30 days in flow-through laboratory aquaria in French Polynesia; (ii) survival and recovery of free-swimming blacktip reef sharks in Australia and French Polynesia following a range of injuries; and (iii) long-term survival following suspected shark-finning activities. Laboratory monitoring, tag-recapture records, telemetry data and photo-identification records suggest that blacktip reef sharks have a high capacity to survive and recover from small or even large and severe wounds. Healing rates, recovery and survival are important factors to consider when assessing impacts of habitat degradation and fishing stress on shark populations. The present study suggests that individual survival may depend more on handling practices and physiological stress rather than the extent of physical injury. These observations also contribute to discussions regarding the ethics of tagging practices used in elasmobranch research and provide baseline healing rates that may increase the accuracy in estimating reproductive timing inferred from mating scars and birth dates for neonatal sharks based on umbilical scar healing status. Cite as: Chin A, Mourier J, Rummer JL (2015) Blacktip reef sharks (Carcharhinus melanopterus) show high capacity for wound healing and recovery following injury. Conserv Physiol 3: doi:10.1093/conphys/cov062.
Conservation physiology

Droits : http://creativecommons.org/licenses/by/
hal-01250745
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745/file/Conserv%20Physiol-2015-Chin-.pdf
DOI : 10.1093/conphys/cov062

Éditeur(s) : Inter-Research
Résumé : We studied the foraging rhythms of green sea turtles Chelonia mydas on the seagrass beds of N'Gouja Bay, Mayotte Island (Comoros Archipelago) with acoustic transmitters and moored listening stations. We monitored 8 tagged turtles (4 probable males, 3 probable females and 1 immature), from 70 to 109 cm curved carapace length (CCL), for durations ranging from 5 to 92 d. The turtles exhibited a regular diel pattern: they foraged mainly during the day (on average 87% of seagrass detections were between 06:00 and 18:00 h) and rested on the inner reef slope during the night. Night time feeding activities were observed on the seagrass bed when the night light was high. The presence of turtles on the seagrass bed at night was significantly correlated with a night light index (r = 0.54, p = 0.002), which included both moon light and cloudiness indices. Behaviour of the only immature individual observed was similar to adult turtles, although it rested more frequently around noon. All turtles displayed a high fidelity to 1 foraging site within the seagrass bed. Acoustic transmitters and permanent listening stations are an appropriate technique for long-term behavioural studies of turtles, with no human interaction with turtles during tracking, and represent a suitable technique to assess the possible effects of environmental changes or human activities upon green turtle behaviour.
Marine Ecology Progress Series (0171-8630) (Inter-Research), 2006 , Vol. 306 , P. 295-302

Droits : Inter-Research 2006
http://archimer.ifremer.fr/doc/2006/publication-3616.pdf
DOI:10.3354/meps306295
http://archimer.ifremer.fr/doc/00000/3616/

Éditeur(s) : HAL CCSD Instituto Oswaldo Cruz, Ministério da Saúde
Résumé : International audience
Leishmania are protozoan parasites that show remarkable diversity, as revealed by the various clinical forms of leishmaniasis, which can range from mild skin lesions to severe metastatic cutaneous/mucosal lesions. The exact nature and extent of Leishmania phenotypic diversity in establishing infection is not fully understood. In order to try to understand some aspects of this diversity, we subcutaneously infected BALB/c mice with first and second generation subclones of a L. amazonensis strain isolated from a patient (BA125) and examined in vivo lesion growth rate and antimony susceptibility. In vivo fast-, medium- and slow-growing subclones were obtained; moreover, fast-growing subclones could generate slow-growing subclones and inversely, revealing the continuous generation of diversity after passage into mice. No antimony-resistant subclone appeared, probably a rare occurrence. By tagging subclone cells with a L. amazonensis genomic cosmid library, we found that only a very small number of founding cells could produce lesions. Leishmania clones transfected with in vivo selected individual cosmids were also diverse in terms of lesion growth rate, revealing the cosmid-independent intrinsic characteristics of each clone. Our results suggest that only a few of the infecting parasites are able to grow and produce lesions; later, within the cell mixture of each lesion, there coexist several parasite populations with different potentialities to grow lesions during the next infection round. This may reflect a sort of programmed heterogeneity of individual parasites, favoring the survival of some individuals in various environmental conditions.
ISSN: 0074-0276

hal-01489007
https://hal-univ-perp.archives-ouvertes.fr/hal-01489007

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Background: Researchers sorely need markers and approaches for biodiversity exploration (both specimen linked and metagenomics) using the full potential of next generation sequencing technologies (NGST). Currently, most studies rely on expensive multiple tagging, PCR primer universality and/or the use of few markers, sometimes with insufficient variability. Methodology/Principal Findings: We propose a novel approach for the isolation and sequencing of a universal, useful and popular marker across distant, non-model metazoans: the complete mitochondrial genome. It relies on the properties of metazoan mitogenomes for enrichment, on careful choice of the organisms to multiplex, as well as on the wide collection of accumulated mitochondrial reference datasets for post-sequencing sorting and identification instead of individual tagging. Multiple divergent organisms can be sequenced simultaneously, and their complete mitogenome obtained at a very low cost. We provide in silico testing of dataset assembly for a selected set of example datasets. Conclusions/Significance: This approach generates large mitogenome datasets. These sequences are useful for phylogenetics, molecular identification and molecular ecology studies, and are compatible with all existing projects or available datasets based on mitochondrial sequences, such as the Barcode of Life project. Our method can yield sequences both from identified samples and metagenomic samples. The use of the same datasets for both kinds of studies makes for a powerful approach, especially since the datasets have a high variability even at species level, and would be a useful complement to the less variable 18S rDNA currently prevailing in metagenomic studies.
ISSN: 1932-6203

hal-01544766
https://hal.archives-ouvertes.fr/hal-01544766
DOI : 10.1371/journal.pone.0051263

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : The original baitboat fishing technique using a permanent association between the fishing boat and the tuna school has been developed by the baitboat fleet of Dakar, Senegal and is also in use in the Canary Islands. This new fishing technique, the result of 20 years of improvements still on, has induced a dramatic increase of the catch yields which, in turn, has sustained the survival and even a recent development of the fleet. This technique is based on the aggregating behaviour of tropical tunas. However, it contains a dynamic component which is not found in tuna fishing on drifting or anchored fads. The specifications of the method, its refinement over the years and its main consequences for the fishery are described together with the school exchanges between baitboats over months even from one year to the next. Based on the analysis of 1228 recoveries from 5500 tagged fish, tuna movements among associated schools, between associated schools and free schools fished by purse seiners and movements in and out of the baitboat fishing grounds help to better understand the dynamics of tunas and schools of this peculiar tuna association. These data show a very high recovery rate, a remarkable tuna fidelity to the original school for all species, a small number of recoveries within the purse seine catch as well as the rare tuna movements outside the baitboat area. The study highlights the complexity and the numerous consequences of tuna and school behaviour.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15304/12637.pdf
http://archimer.ifremer.fr/doc/00042/15304/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : In order to analyze the large-scale structure of the genome of Norway spruce (Picea abies Karst.), a pseudo-testcross genetic linkage map was built using markers of six different types, belonging to the low (amplified fragment length polymorphisms, simple sequence repeats) or high (sequence-specific amplified polymorphisms, inter-retrotransposon amplified polymorphisms) copy-number fraction of the genome, and including expressed region-derived markers (expressed sequence tag polymorphisms). Twenty seven and 23 linkage groups of at least four markers were obtained for the female and the male parent maps, respectively. A subset of these linkage groups coalesced into 13 bi-parental linkage groups through markers shared between the two maps. This map was used to investigate the frequency of each marker type over chromosomes and the distribution of marker types relative to each other, using autocorrelation techniques. Our results show that, while the composition of chromosomes is homogeneous, low- and high-copy-number markers tend to occupy separate regions of the linkage groups, and that expressed sequences are preferentially associated with microsatellites and separated from retrotransposons. These results indicate that the spatial structure of Norway spruce chromosomes is not homogeneous
ISSN: 1614-2942

hal-01032033
https://hal.archives-ouvertes.fr/hal-01032033
DOI : 10.1007/s11295-005-0012-2

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
A hybrid linear ion-trap Fourier-transform ion cyclotron resonance mass spectrometer was used for top-down characterization of the abundant human salivary Cystatins, including S, S1, S2, SA, SN, C, and D, using collisionally activated dissociation (CAD) after chromatographic purification of the native, disulfide intact proteins from saliva. Post-translational modifications and protein sequence polymorphisms arising from single nucleotide polymorphisms (SNPs) were assigned from precursor and product ion masses at a tolerance of 10 ppm allowing confident identification of individual intact mass tags. Cystatins S, S1, S2, SA and SN were cleaved of a N-terminal 20 amino-acid signal peptide, and Cystatin C a 26-residue peptide, to yield a generally conserved N-terminus. In contrast, Cystatin D isoforms with 24 and 28 amino-acid residue N-terminal truncations were found such that their N-termini were not conserved. Cystatin S1 was phosphorylated at Ser3, while S2 was phosphorylated at Ser1 and Ser3 of the mature protein, in agreement with previous work. Both Cystatin D isoforms carried the polymorphism C46R (SNP: rs1799841). The 14328 Da isoform of Cystatin SN previously assigned with polymorphism P31L due to a SNP (rs2070856) was found only in whole saliva. Parotid secretions contained no detectable Cystatins while whole saliva largely mirrored the contents of submandibular/sublingual (SMSL) secretions. Top-down high-resolution mass spectrometry is a powerful tool for the identification and characterization of potential protein biomarkers in saliva.
ISSN: 1044-0305

hal-00677483
https://hal.archives-ouvertes.fr/hal-00677483
DOI : 10.1016/j.jasms.2010.01.025

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together, while communities from the same site but different seasons were unrelated. Both DNA and RNA-based surveys provided similar relative abundances for most class-level taxonomic groups. Yet, particular groups were overrepresented in one of the two templates, such as marine alveolates (MALV)-I and MALV-II that were much more abundant in DNA surveys. Overall, the groups displaying the highest relative contribution were Dinophyceae, Diatomea, Ciliophora and Acantharia. Also, well represented were Mamiellophyceae, Cryptomonadales, marine alveolates and marine stramenopiles in the picoplankton, and Monadofilosa and basal Fungi in sediments. Our extensive and systematic sequencing of geographically separated sites provides the most comprehensive molecular description of coastal marine protist diversity to date.
ISSN: 1462-2912

hal-01191731
http://hal.upmc.fr/hal-01191731
http://hal.upmc.fr/hal-01191731/document
http://hal.upmc.fr/hal-01191731/file/Massana_2015_Marine_protist.pdf
DOI : 10.1111/1462-2920.12955

Éditeur(s) : HAL CCSD Wiley Open Access
Résumé : International audience
Coral disease outbreaks have increased over the last three decades, but their causal agents remain mostly unclear (e.g., bacteria, viruses, fungi, protists). This study details a 14-month-long survey of coral colonies in which observations of the development of disease was observed in nearly half of the sampled colonies. A bimonthly qPCR method was used to quantitatively and qualitatively evaluate Symbiodinium assemblages of tagged colonies, and to detect the presence of Vibrio spp. Firstly, our data showed that predisposition to disease development in general, and, more specifically, infection by Vibrio spp. in Acropora cytherea depended on which clades of Symbiodinium were harbored. In both cases, harboring clade D rather than A was beneficial to the coral host. Secondly, the detection of Vibrio spp. in only colonies that developed disease strongly suggests opportunistic traits of the bacteria. Finally, even if sporadic cases of switching and probably shuffling were observed, this long-term survey does not suggest specific-clade recruitment in response to stressors. Altogether, our results demonstrate that the fitness of the coral holobiont depends on its initial consortium of Symbiodinium, which is distinct among colonies, rather than a temporary adaptation achieved through acquiring different Symbiodinium clades.
ISSN: 2045-7758

hal-01346931
https://hal-uvsq.archives-ouvertes.fr/hal-01346931
https://hal-uvsq.archives-ouvertes.fr/hal-01346931/document
https://hal-uvsq.archives-ouvertes.fr/hal-01346931/file/2016%20Rouze%20EcolEvol.pdf
DOI : 10.1002/ece3.1895

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little isknown about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals.Results: Out of the 358 and 346 expressed sequence tags (ESTs) obtained by conducting two SSH libraries respectively, the expression patterns of 37 genes were tested with a real-time quantitative PCR (RT-qPCR) approach by pooling five individuals of each phenotype. The expression of 11 genes was subsequently estimated for each individual in order to detect inter-individual variation. Our results suggest that the color of the nacre is partiallyunder the influence of genes involved in the biomineralization of the calcitic layer. A few genes involved in the formation of the aragonite tablets of the nacre layer and in the biosynthesis chain of melanin also showed differential expression patterns. Finally, high variability in gene expression levels were observed within the black phenotypes.Conclusions: Our results revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin, suggesting that color polymorphism takes place at different levels in the shell structure. The high variability of gene expression found within black phenotypes suggests that the present work should serve as a basis for future studies exploringmore thoroughly the expression patterns of candidate genes within black phenotypes with different dominant iridescent colors.
ISSN: 1471-2164

hal-01260141
https://hal.archives-ouvertes.fr/hal-01260141
https://hal.archives-ouvertes.fr/hal-01260141/document
https://hal.archives-ouvertes.fr/hal-01260141/file/Lemer-2015-BMCGenom-Identification.pdf
DOI : 10.1186/s12864-015-1776-x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Marine Protected Areas (MPAs), if well designed and managed, can produce conservation benefits to fish assemblages within no-take zones and fishery benefits in neighboring areas through ‘spillover’. However, although plenty of studies have provided evidence of the benefits produced within MPA boundaries, overall benefits to local fisheries, especially via spillover, seem to be still unclear. Because of the lost fishing grounds following an MPA establishment, local fishermen usually oppose MPAs. There is, therefore, the urgent need for a better understanding of the mechanism(s) through which MPAs can export fishable fish biomass towards adjacent fished areas, a process that could counterbalance the loss of fishing grounds. Here we review the literature on spillover for refining the terminology, detailing the underlying mechanisms and identifying both the existing and needed methodological approaches to measure spillover. Operationally, two types of spillover should be considered: ecological spillover (i.e. the net export of juvenile, subadult and adult biomass from MPAs outwards driven by density-dependent processes) and the fishery spillover (i.e. the proportion of this biomass that can be fished, taking into account regulations and accessibility). Underwater visual census and tagging/tracking may allow getting evidence of ecological spillover, while experimental catch data are essential to assess and monitor fishery spillover, which is the main component of MPAs that can provide direct benefit to local fisheries.
ISSN: 1617-1381

hal-01325478
https://hal-univ-perp.archives-ouvertes.fr/hal-01325478
DOI : 10.1016/j.jnc.2016.04.004

Éditeur(s) : Academic Press Ltd- Elsevier Science Ltd
Résumé : Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line expression of an extracellular superoxide dismutase, inhibitor of apoptosis (OeIAP), Fas ligand (OeFas-ligand) and Cathepsin B was significantly increased, whilst cyclophilin B appeared significantly decreased in resistant oysters. Considering their great interest for further studies, the open reading frames of the OeFas-ligand and OeIAP were completely sequenced. Highlights ► Bonamia ostreae is an intra-haemocytes parasite. ► Flat oyster Ostrea edulis, is the natural host of B. ostreae. ► In this study we have studied genes expressed in response to the parasite. ► Extracellular superoxide dismutase (OeEcSOD) is up regulated in resistant haemocyte. ► Genes involved in apoptosis is up regulated in resistant haemocytes from flat oyster.
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2012-06 , Vol. 32 , N. 6 , P. 958-968

Droits : 2012 Elsevier Ltd. All rights reserved.
http://archimer.ifremer.fr/doc/00071/18218/15790.pdf
DOI:10.1016/j.fsi.2012.01.026
http://archimer.ifremer.fr/doc/00071/18218/

Éditeur(s) : HAL CCSD Wiley Open Access
Résumé : International audience
Coral disease outbreaks have increased over the last three decades, but their causal agents remain mostly unclear (e.g., bacteria, viruses, fungi, protists). This study details a 14-month-long survey of coral colonies in which observations of the development of disease was observed in nearly half of the sampled colonies. A bimonthly qPCR method was used to quantitatively and qualitatively evaluate Symbiodinium assemblages of tagged colonies, and to detect the presence of Vibrio spp. Firstly, our data showed that predisposition to disease development in general, and, more specifically, infection by Vibrio spp. in Acropora cytherea depended on which clades of Symbiodinium were harbored. In both cases, harboring clade D rather than A was beneficial to the coral host. Secondly, the detection of Vibrio spp. in only colonies that developed disease strongly suggests opportunistic traits of the bacteria. Finally, even if sporadic cases of switching and probably shuffling were observed, this long-term survey does not suggest specific-clade recruitment in response to stressors. Altogether, our results demonstrate that the fitness of the coral holobiont depends on its initial consortium of Symbiodinium, which is distinct among colonies, rather than a temporary adaptation achieved through acquiring different Symbiodinium clades.
ISSN: 2045-7758

hal-01270779
https://hal-univ-perp.archives-ouvertes.fr/hal-01270779
https://hal-univ-perp.archives-ouvertes.fr/hal-01270779/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01270779/file/41080.pdf
DOI : 10.1002/ece3.1895

Éditeur(s) : HAL CCSD National Academy of Sciences
Résumé : International audience
We applied single-molecule photobleaching to investigate the stoichiometry of human Orai1 and Orai3 channels tagged with eGFP and expressed in mammalian cells. Orai1 was detected predominantly as dimers under resting conditions and as tetramers when coexpressed with C-STIM1 to activate Ca(2+) influx. Orai1 was also found to be tetrameric when coexpressed with STIM1 and evaluated following fixation. We show that fixation rapidly causes release of Ca(2+), redistribution of STIM1 to the plasma membrane, and STIM1/Orai1 puncta formation, and may cause the channel to be in the activated state. Consistent with this possibility, Orai1 was found predominantly as a dimer when coexpressed with STIM1 in living cells under resting conditions. We further show that Orai3, like Orai1, is dimeric under resting conditions and is predominantly tetrameric when activated by C-STIM1. Interestingly, a dimeric Orai3 stoichiometry was found both before and during application of 2-aminoethyldiphenyl borate (2-APB) to activate a nonselective cation conductance in its STIM1-independent mode. We conclude that the human Orai1 and Orai3 channels undergo a dimer-to-tetramer transition to form a Ca(2+)-selective pore during store-operated activation and that Orai3 forms a dimeric nonselective cation pore upon activation by 2-APB.
ISSN: 0027-8424

hal-00873833
https://hal.archives-ouvertes.fr/hal-00873833
DOI : 10.1073/pnas.1114814108
PUBMED : 21987805

Éditeur(s) : HAL CCSD Elsevier
Résumé : TAG LITHO
In this study we attempt to understand the water storage variations in a karst aquifer on the Larzac Plateau (South of France) using absolute gravimetry. On this karst system, water input is exclusively rainfall and draining occurs at the Durzon perennial spring in a karstic valley. Our basis assumption is that water storage in the vadose zone (epikarst and infiltration zone) has a significant effect on surface gravity through Newtonian attraction. The karst aquifer is hence being monitored since January 2006 with monthly absolute gravity measurements at three sites. The gravity measurements are corrected for regional scale gravity using water storage models in order to obtain gravity variations related exclusively to the local water storage variations. The gravity variations exhibit an important seasonal component (10-15 mu gal corresponding to a variation of equivalent water stab thickness of similar to 24-36 cm). Water storage variation seen by gravimetry is not spatially uniform on the studied karst system, corroborating current geomorphologic observations and interpretations. Finally, we use a global mass balance consideration linking rainfall, evapotranspiration and spring discharge to provide a conceptual framework for the understanding of observed gravity variations.
ISSN: 0022-1694

hal-00411884
https://hal.archives-ouvertes.fr/hal-00411884
DOI : 10.1016/j.jhydrol.2008.06.020