Éditeur(s) : Public Library Science
Résumé : Background: Reconstructing the history of divergence and gene flow between closely-related organisms has long been a difficult task of evolutionary genetics. Recently, new approaches based on the coalescence theory have been developed to test the existence of gene flow during the process of divergence. The deep sea is a motivating place to apply these new approaches. Differentiation by adaptation can be driven by the heterogeneity of the hydrothermal environment while populations should not have been strongly perturbed by climatic oscillations, the main cause of geographic isolation at the surface. Methodology/Principal Finding: Samples of DNA sequences were obtained for seven nuclear loci and a mitochondrial locus in order to conduct a multi-locus analysis of divergence and gene flow between two closely related and hybridizing species of hydrothermal vent mussels, Bathymodiolus azoricus and B. puteoserpentis. The analysis revealed that (i) the two species have started to diverge approximately 0.760 million years ago, (ii) the B. azoricus population size was 2 to 5 time greater than the B. puteoserpentis and the ancestral population and (iii) gene flow between the two species occurred over the complete species range and was mainly asymmetric, at least for the chromosomal regions studied. Conclusions/Significance: A long history of gene flow has been detected between the two Bathymodiolus species. However, it proved very difficult to conclusively distinguish secondary introgression from ongoing parapatric differentiation. As powerful as coalescence approaches could be, we are left by the fact that natural populations often deviates from standard assumptions of the underlying model. A more direct observation of the history of recombination at one of the seven loci studied suggests an initial period of allopatric differentiation during which recombination was blocked between lineages. Even in the deep sea, geographic isolation may well be a crucial promoter of speciation.
Plos One (1932-6203) (Public Library Science), 2009-08 , Vol. 4 , N. 8 , P. 1-15

Droits : 2009 Faure et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://archimer.ifremer.fr/doc/00129/24012/21972.pdf
DOI:10.1371/journal.pone.0006485
http://archimer.ifremer.fr/doc/00129/24012/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The South Liaodong Peninsula massif is the easternmost Mesozoic Metamorphic Core Complex, recognized in Eastern China. It provides a good example of the combination of ductile shearing, synkinematic plutonism and polyphase exhumation. The Jurassic granodioritic plutons, located at the footwall of the detachment normal fault, and dated here at ca 160 Ma, recorded two different phases of cooling. A slow cooling regime of about 3-10°C/my prevailing before 122 Ma, was followed by a significant increase in cooling rate of about 40-55°C/my after that time. By contrast, a single fast cooling path was recorded by the Cretaceous monzogranite situated in the footwall of the detachment normal fault. This result indicates that the Jurassic and Cretaceous plutons recorded different exhumation processes: a Jurassic slow or negligible exhumation and a Cretaceous fast one assisted by normal faulting. These two cooling stages correspond to distinct geodynamic processes during the Jurassic and Cretaceous. Extensional tectonics seems not significant before Early Cretaceous. The second stage, dominated by an extensional regime which develops after ca 120 Ma, is tentatively correlated to the lithosphere removal of the North China Craton.
ISSN: 1367-9120

Droits : http://creativecommons.org/licenses/by-nc-nd/
insu-00528171
https://hal-insu.archives-ouvertes.fr/insu-00528171
https://hal-insu.archives-ouvertes.fr/insu-00528171/document
https://hal-insu.archives-ouvertes.fr/insu-00528171/file/Lin-JAsianEarthSciences-2010.pdf
DOI : 10.1016/j.jseaes.2010.09.007

Éditeur(s) : Academic Press Ltd- Elsevier Science Ltd
Résumé : Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line expression of an extracellular superoxide dismutase, inhibitor of apoptosis (OeIAP), Fas ligand (OeFas-ligand) and Cathepsin B was significantly increased, whilst cyclophilin B appeared significantly decreased in resistant oysters. Considering their great interest for further studies, the open reading frames of the OeFas-ligand and OeIAP were completely sequenced. Highlights ► Bonamia ostreae is an intra-haemocytes parasite. ► Flat oyster Ostrea edulis, is the natural host of B. ostreae. ► In this study we have studied genes expressed in response to the parasite. ► Extracellular superoxide dismutase (OeEcSOD) is up regulated in resistant haemocyte. ► Genes involved in apoptosis is up regulated in resistant haemocytes from flat oyster.
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2012-06 , Vol. 32 , N. 6 , P. 958-968

Droits : 2012 Elsevier Ltd. All rights reserved.
http://archimer.ifremer.fr/doc/00071/18218/15790.pdf
DOI:10.1016/j.fsi.2012.01.026
http://archimer.ifremer.fr/doc/00071/18218/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In East Asia, widespread extensional sedimentary basins together with the close association of Metamorphic Core Complexes and magmatism are prominent features of the large-scale wide rift system developed during late Mesozoic times. This region thus appears as a proper place to study continental extension as well as questions about the link between continental extension and magmatism. This paper primarily provides pioneer exhumation time-constraints on the Linglong MCC (Jiaodong Peninsula) including cooling and deformation ages. MCC cooling at mid-crustal levels occurred at ca. 143 Ma. Besides, last ductile deformation occurred at ca. 134 Ma while final exhumation stages, under brittle 2 conditions, occurred as late as ca. 128 Ma. Continuous crustal stretching were then recorded by the emplacement of a synkinematic pluton in the upper crust at ca. 128 Ma that cooled fast below an intracrustal shear zone crossing the ductile-brittle transition at ca. 123 Ma. The ca. 120-118 Ma age cluster is ascribed to the fast cooling of undeformed plutons marking the end of extension that lasted, in the area, over a minimum period of ca. 30 My. Combining other MCC exhumation constraints and the onset of subsidence in the sedimentary basins, total duration of late Mesozoic extension in East Asia could be estimated at ca. 60 My, related with a rather long process for extension from 160 to at least 100 Ma. East Asian continental extension is heterogeneously distributed in space and time as revealed by fundamental differences between two end-member classes of migmatite-cored MCC already described in other wide rift systems. Extension seems to have first favoured partial melting which subsequently, in turn, maintained continental extension.
ISSN: 1342-937X

insu-00752551
https://hal-insu.archives-ouvertes.fr/insu-00752551
https://hal-insu.archives-ouvertes.fr/insu-00752551/document
https://hal-insu.archives-ouvertes.fr/insu-00752551/file/Charles_et_al_Gondwana_Research_2012.pdf
DOI : 10.1016/j.gr.2012.10.011

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In this contribution we present a reconstruction of the overall lithotectonic architecture, from inner zones to external ones, of the Paleoproterozoic Trans North China Orogen, within the North China Craton. Moreover, forward thermobarometrical modeling on a kyanite-bearing gneiss yields a reliable prograde P-T-t-D path. In addition, 40Ar/39Ar dating on rocks from distinct litho-tectonic units helps us to distinguish several tectono-metamorphic events during the orogenic development. Considering these results, we propose a geodynamic model involving three cratonic blocks, namely the Western, Fuping and Eastern Blocks, separated by two oceans, the Lüliang and Taihang Oceans. The opening of oceanic basins occurred around 2.2-2.3 Ga. After the westward subductions of oceanic lithosphere, the Trans-North-China Orogen was built up through a polyphase tectonic evolution within the period 1900-1800 Ma. The first event (D1) corresponded to the emplacement of lower and upper nappes herein called the Orthogneiss-and-Volcanites Unit (OVU) and the Low-Grade-and-Mafic Unit (LGMU), respectively. The syn-metamorphic D1 deformation (1880 ± 10 Ma) is characterized by a NW-SE stretching and mineral lineation with a top-to-the SE sense of shear. During ongoing compression of the thickening orogenic crust, a second deformation event D2 (1850 ± 10 Ma) was responsible for (1) syn-anatectic lateral flow and exhumation of the orogenic root and (2) folding of the middle and upper parts of the orogenic wedge that consequently acquired a fan-type geometry. The late D3 (1830 ± 10 Ma) and D4 (1810 ± 10 Ma) events are related to late-orogenic normal and strike-slip shearing, respectively. In our present state of knowledge, the Paleoproterozoic Trans-North China Orogen might be regarded as the assemblage of two continent-continent collisional belts, both of which are characterized by nappe stacking accommodated by top-to-the E/SE ductile shearing. Continental subduction, crustal thickening, partial melting of overthickened crust, exhumation of HP rocks and deposition of syn-orogenic detrital basins are typical features of modern collisional-type orogens.
ISSN: 0301-9268

Droits : info:eu-repo/semantics/OpenAccess
insu-00628119
https://hal-insu.archives-ouvertes.fr/insu-00628119
https://hal-insu.archives-ouvertes.fr/insu-00628119/document
https://hal-insu.archives-ouvertes.fr/insu-00628119/file/Trap-PrecambrianResearch-2011.pdf
DOI : 10.1016/j.precamres.2011.09.008

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mechanism and kinematics of the Late Mesozoic continental extension event of eastern Asia are still debated. In order to better constrain its evolution, two granodioritic plutons of the Jiaodong Peninsula have been chosen as targets for a time-constrained palaeomagnetic study. Indeed, plutons are devoid of visible deformation, did not experience rotation along horizontal axis and are precisely dated by U/Pb and 40Ar/39Ar methods. Multidomain (MD) magnetite has been identified as the principal magnetic remanent carrier. The interpolation of existing and new U/Pb and 40Ar/39Ar ages revealed that characteristic remanent magnetisation was acquired in a narrow range of 116 ± 2 Ma. Twenty out of 27 sites present stable magnetic directions calculated from high-temperature or high-coercive components. The observations of the solo normal magnetic polarity for this palaeomagnetic collection and of the magnetic remanent age consistent with the Cretaceous Normal Superchron (CNS) argue that the characteristic magnetic remanence may be considered as primary. Detailed field observations of the intrusive relationship between the plutons and country rocks and Anisotropy of Magnetic Susceptibility (AMS) study reveal the absence of the subsequent deformation of plutons, or rigid rotation of plutons along a horizontal axis. Two palaeomagnetic poles have been therefore calculated from these plutons. Among 12 out 15 Cretaceous palaeomagnetic poles, including the two new poles provided by this study, from the Jiaodong Peninsula and on both sides of Tan-Lu and Muping-Jimo faults are statistically consistent. As a result, the Jiaodong Peninsula behaved as a rigid block as internal deformation appears negligible. The remaining three derived poles are probably due to the secular variation or/and the vicinity of fault zones near of the palaeomagnetic sampling site. Thus, they can not be applied to the peninsula-scaled tectonics. Comparison of these time52 constrained Cretaceous palaeomagnetic results of the Jiaodong Peninsula with those of North China Block (NCB) indicate that the Jiaodong Peninsula was rigidly attached to NCB since, 3 at least, the Cretaceous as residual difference is clearly insignificant with respect to the error brackets. These new palaeomagnetic results confirm that the relative clockwise (CW) rotation of Eastern Liaoning-Korea Block (ELKB) with respect to NCB does not affect the Jiaodong Peninsula as a rigid block, nevertheless, some tectonic structures have been reactivated since Cenozoic and local rotations could be produced. This palaeomagnetic study reveals that the Late Mesozoic extension in Eastern Asia is heterogeneously expressed in time and space. The complete understanding of the mechanism(s) of this extensional event needs further multidisciplinary investigations.
ISSN: 0031-9201

insu-00599546
https://hal-insu.archives-ouvertes.fr/insu-00599546
https://hal-insu.archives-ouvertes.fr/insu-00599546/document
https://hal-insu.archives-ouvertes.fr/insu-00599546/file/Charles_et_al_PEPI2011.pdf
DOI : 10.1016/j.pepi.2011.05.006

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : New structural, microstructural, and 40Ar/39Ar data from the NW Massif Central (France) provide additional constraints on the timing and tectonic setting of late Variscan granite magmatism. Previous studies had emphasized the role of late orogenic extension in the emplacement of granite plutons in the Limousin region. In contrast, the new data set is consistent with syntectonic emplacement of magma in a dextral simple shear active from 350 to 300 Ma in a transpressional regime. As an alternative hypothesis to late orogenic extension, we propose that magmas migrated into tensional bridges between active P shears associated with a lithospheric shear zone comparable to a pop-up structure. The Galician region, in the western end of the Ibero-Armorican tectonic arc, exhibits major left-lateral ductile shear zones which can be interpreted as conjugate structures to the Limousin and Armorican shear zones. Citation: Ge´belin, A., M. Brunel, P. Monie´, M. Faure, and N. Arnaud (2007), Transpressional tectonics and Carboniferous magmatism in the Limousin, Massif Central, France: Structural and 40Ar/39Ar investigations
ISSN: 0278-7407

hal-00104212
https://hal-insu.archives-ouvertes.fr/hal-00104212
https://hal-insu.archives-ouvertes.fr/hal-00104212/document
https://hal-insu.archives-ouvertes.fr/hal-00104212/file/2005TC001822.pdf
DOI : 10.1029/2005TC001822

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Investigation of an eclogite xenolith, discovered in a Cretaceous granite from the Central Domain of the Dabieshan massif in eastern China, yields new petrological insights into the high to ultrahigh-pressure metamorphism, experienced by the Qinling-Dabie orogen. Prior to inclusion as a xenolith in the granite during the Early Cretaceous, this eclogite xenolith had recorded a complex metamorphic evolution that complies with subduction and exhumation processes experienced by the continental crust of the South China Block. Well-preserved mineral parageneses substantiate the prograde and retrograde stages revealed by inclusions in porphyroblastic garnet and zoned minerals such as garnet, comphacite and amphibole in the matrix. The relatively low P/T re-equilibration during a latemetamorphic stage was textually inferred by the presence of aluminous and calcic-subcalcic amphiboles such as katophorite, edenite, taramite and pargasite as main matrix phases. According to our U/Pb, Rb/Sr and new 40Ar/39Ar geochronological results, namely 109 ± 1 and 112 ± 2 Ma plateau ages for muscovite and amphiboles, respectively, two successive but distinct cooling stages account for the thermal history of the granite–migmatite gneiss dome that forms the Central Dabieshan Domain. We argue that prior to the Cretaceous doming, the Central Dabieshan Domain experienced a tectono-metamorphic evolution similar to that observed in the high-pressure to ultra high-pressure units developed in the Southern Dabieshan Domain and Hong'an massif.
ISSN: 1437-3254

hal-00104224
https://hal-insu.archives-ouvertes.fr/hal-00104224
https://hal-insu.archives-ouvertes.fr/hal-00104224/document
https://hal-insu.archives-ouvertes.fr/hal-00104224/file/Lin-IntJEarthSciences-2007.pdf
DOI : 10.1007/s00531-006-0129-z