Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Le présent document étudie à l’aide d’un modèle à composantes inobservables, la faisabilité d’une union économique et monétaire (UEM) entre Haïti et la République dominicaine, deux pays partageant géographiquement le même territoire, mais dont l’histoire est parsemée de conflits et de résultats économiques divergents notamment en matière de croissance. Pour autant, peut-on, malgré les obstacles du passé et les incompréhensions du présent envisager un processus d’intégration, allant jusqu’à la création entre les deux pays d’une union économique et monétaire ? L’objectif de l’étude est d’identifier et d’évaluer le degré d’(a) symétrie des chocs macroéconomiques au sein des économies en question afin d’apprécier la pertinence ou non de l’instauration d’une UEM entre elles. Les résultats révèlent la fragilité aujourd’hui de ce processus d’intégration en raison de la faible convergence des politiques économiques entre les deux pays. La désagrégation des chocs issue de notre modèle confirme cette asymétrie des comportements, asymétrie expliquée par le faible poids des composantes communes notamment par les chocs de politique économique domestiques. Cependant, l’endogénéité de la plupart des critères retenus plaide en faveur d’un agenda visant à créer à moyen terme les conditions d’une plus grande convergence entre les politiques économiques des deux pays, conditions qui, une fois réunies, faciliteraient pour l’avenir leur implication dans un schéma d’intégration plus poussé par rapport au présent.
This paper uses an unobserved component model to analyse the feasibility of economic and monetary union (EMU) between Haiti and Dominican Republic, two countries sharing the same island but whose history is one conflict and divergent economic prospects. In spite of these historical obstacles, is it possible to plan an economic and monetary union among these economies? The objective of this paper is to provide a framework to identify and evaluate the asymmetry degree of macroeconomic shocks within these economies in order to appreciate the viability of this project, also the nature of these potential benefits and costs. The results show the fragility of this process because of the weak economic policy convergence among these countries. The shocks disintegration stemming from our model confirm these results and suggests that at this stage, several economic criteria are not satisfied for the two countries to fully benefit from an economic and monetary union. However, the endogeneity of most of these criteria militates in favour of an agenda for progressive integration between them.
Haïti
République dominicaine

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.7188
http://journals.openedition.org/etudescaribeennes/7188

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699
Background and Aims Plastic tree-shelters are increasingly used to protect tree seedlings against browsing animals and herbicide drifts. The biomass allocation in young seedlings of deciduous trees is highly disturbed by common plastic tree-shelters, resulting in poor root systems and reduced diameter growth of the trunk. The shelters have been improved by creating chimney-effect ventilation with holes drilled at the bottom, resulting in stimulated trunk diameter growth, but the root deficit has remained unchanged. An experiment was set up to elucidate the mechanisms behind the poor root growth of sheltered Prunus avium trees. Methods Tree seedlings were grown either in natural windy conditions or in tree-shelters. Mechanical wind stimuli were suppressed in ten unsheltered trees by staking. Mechanical stimuli (bending) of the stem were applied in ten sheltered trees using an original mechanical device. Key Results Sheltered trees suffered from poor root growth, but sheltered bent trees largely recovered, showing that mechano-sensing is an important mechanism governing C allocation and the shoot-root balance. The use of a few artificial mechanical stimuli increased the biomass allocation towards the roots, as did natural wind sway. It was demonstrated that there was an acclimation of plants to the imposed strain. Conclusions This study suggests that if mechanical stimuli are used to control plant growth, they should be applied at low frequency in order to be most effective. The impact on the functional equilibrium hypothesis that is used in many tree growth models is discussed. The consequence of the lack of mechanical stimuli should be incorporated in tree growth models when applied to environments protected from the wind (e.g. greenhouses, dense forests).
ISSN: 0305-7364

hal-01032056
https://hal.archives-ouvertes.fr/hal-01032056
DOI : 10.1093/aob/mcn054

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Signalling through the Wnt family of secreted proteins originated in a common metazoan ancestor and greatly influenced the evolution of animal body plans. In bilaterians, Wnt signalling plays multiple fundamental roles during embryonic development and in adult tissues, notably in axial patterning, neural development and stem cell regulation. Studies in various cnidarian species have particularly highlighted the evolutionarily conserved role of the Wnt/b-catenin pathway in specification and patterning of the primary embryonic axis. However in another key non-bilaterian phylum, Ctenophora, Wnts are not involved in early establishment of the body axis during embryogenesis. We analysed the expression in the adult of the ctenophore Pleurobrachia pileus of 11 orthologues of Wnt signalling genes including all ctenophore Wnt ligands and Fz receptors and several members of the intracellular b-catenin pathway machinery. All genes are strongly expressed around the mouth margin at the oral pole, evoking the Wnt oral centre of cnidarians. This observation is consistent with primary axis polarisation by the Wnts being a universal metazoan feature, secondarily lost in ctenophores during early development but retained in the adult. In addition, local expression of Wnt signalling genes was seen in various anatomical structures of the body including in the locomotory comb rows, where their complex deployment suggests control by the Wnts of local comb polarity. Other important contexts of Wnt involvement which probably evolved before the ctenophore/ cnidarian/bilaterian split include proliferating stem cells and progenitors irrespective of cell types, and developing as well as differentiated neuro-sensory structures.
ISSN: 1932-6203

hal-01544483
https://hal.archives-ouvertes.fr/hal-01544483
DOI : 10.1371/journal.pone.0084363

Éditeur(s) : HAL CCSD Wiley
Résumé :  
The complex structure of tree bark reflects its many functions, which include structural support as well as defence against fire, pests and pathogens. Thick bark, however, might limit respiration by the living tissues of the trunk. Nevertheless, little research has addressed community-level variation in bark thickness, and to the best of our knowledge, no one has tested multiple hypotheses to explain variation in bark thickness. We conducted an extensive survey of bark thickness within and among species of trees in the tropical rain forests of French Guiana. Trunk bark thickness increased by 1.2 mm per 10 cm increase in stem diameter, and varied widely at all taxonomic levels. Mean trunk bark thickness was 4.5 mm (range: 0.5-29 mm), which was less that found in two Amazonian rain forests in previous studies. This survey of bark thickness should be of use for forest management since tree survival through fire is strongly predicted by bark thickness. We combined the survey data with multiple datasets to test several functional hypotheses proposed to explain variation in bark thickness. We found bark to provide an average of 10% of the flexural rigidity of tree stems, which was substantially less than that found in the only other study of bark stiffness. Bark thickness was uncorrelated with species' association with fire-prone habitats, suggesting that the influence of fire on bark thickness does not extend into moist Forests. There was also little evidence that bark thickness is affected by its function as a defence against herbivory. Nor was there evidence that thick bark limits trunk respiration. A re-analysis of previously collected anatomical data indicated that variation in rhytidome (non-conducting outer bark) thickness explains much of the variation in overall bark thickness. As rhytidome is primarily involved in protecting the living tissues of the trunk, we suggest that bark thickness is driven mostly by its defensive function. Functional explanations for the variation in bark thickness were not clear-cut. Nevertheless, this study provides a foundation for further investigation of the functional bases of bark in tropical trees.
ISSN: 0269-8463

hal-01032124
https://hal.archives-ouvertes.fr/hal-01032124
DOI : 10.1111/j.1365-2435.2010.01736.x

Éditeur(s) : HAL CCSD Mont-Saint-Aignan : Publications des Universités de Rouen et du Havre
Résumé : International audience
The literature on the history of the Paralympics movement tends to present the British games of Stoke Mandeville as the unique origin of the movement and neurosurgeon, Guttman, as a founder hero. Yet, in France initiatives were carried out outside the British dynamics of sports for physically handicapped. They resulted in a range of sports offers stemming out of a self-organized movement created by and for civilian disabled and war-disabled. This self-organization of physically handicapped people, quickly took on a sporting dimension, distinguishing itself from the organizations doctors working in functional rehabilitation services would propose to their patients in the United Kingdom. The French movement quickly took part into the national sports movement. It got structured with a view to include all disabled people into the sporting sphere, then went further into the process of extension and sportivation within international standards. This study builds on a set of data drawn from various revues from different associations of sports movement for French physically disabled from 1954 to 1972. Interviews with major French leaders have also been inserted. We privileged internal sources to focus on the analysis of self-organization dynamics.
La littérature sur l’histoire du mouvement paralympique tend à faire des Jeux britanniques de Stoke Mandeville l’origine unique du mouvement, et du neurochirurgien Guttmann un héros fondateur. Or en France, des initiatives se structurent en dehors de la dynamique britannique du sport pour handicapés physiques. Elles produisent une offre sportive à partir d’une auto-organisation créée par et pour des mutilés de guerre et invalides civils.Cette auto-organisation d’individus handicapés physiques se démarque rapidement de l’organisation proposée par les médecins dans les services de réadaptation fonctionnelle comme au Royaume-Uni, en s’orientant vers une dimension sportive. En s’appuyant sur des réseaux militaires, civils et politiques, le mouvement français participe rapidement au mouvement sportif national. Il se structure alors à partir d’un objectif d’intégration de tous les publics déficients à la sphère sportive, et porte cette volonté d’ouverture et de sportivisation dans les instances internationales. Cette étude s’appuie sur un corpus de données issues des revues des différentes associations du mouvement sportif pour handicapés physiques français de 1954 à 1972, enrichi d’entretiens avec les principaux dirigeants français. Le privilège accordé aux sources internes correspond au choix de centrer l’analyse sur la dynamique d’auto-organisation.
ISSN: 1999-8589

hal-01681465
https://hal.archives-ouvertes.fr/hal-01681465

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Here, the first description of an epsilon sulfur-oxidizing bacterium from sulfide-rich sediments of marine mangrove in Caribbean is reported. According to TEM, this new strain contains intracytoplasmic large internal sulfur granules as confirmed by EDXS analyses performed using ESEM. The sulfur distribution obtained for this sulfur-oxidizing bacterial strain allows to conclude that elemental sulfur is formed as an intermediate oxidation product and stored intracellularly. According to conventional SEM observations, bacterial cells are ovoid, extremely motile by lophotrichous flagella. Phylogenetic analyses based on the analysis of partial sequence of 16S rDNA confirm that such bacterial strain belongs to the Thiovulum cluster and could be a representative of a new species in this poorly studied genus of sulfur-oxidizing free-living bacteria. Thus, reduced sediment of marine mangrove represents sulfide-rich environment sustaining development of both gamma and epsilon sulfur-oxidizing Proteobacteria
ISSN: 0378-1097

hal-01682113
https://hal.univ-antilles.fr/hal-01682113
DOI : 10.1093/femsle/fnx172

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : We examined tree-soil habitat associations in lowland forest communities at Paracou, French Guiana. We analyzed a large dataset assembling six permanent plots totaling 37.5 ha, in which extensive LIDAR-derived topographical data and soil chemical and physical data have been integrated with precise botanical determinations. Map of relative elevation from the nearest stream summarized both soil fertility and hydromorphic characteristics, with seasonally inundated bottomlands having higher soil phosphate content and base saturation, and plateaus having higher soil carbon, nitrogen and aluminum contents. We employed a statistical test of correlations between tree species density and environmental maps, by generating Monte Carlo simulations of random raster images that preserve autocorrelation of the original maps. Nearly three fourths of the 94 taxa with at least one stem per ha showed a significant correlation between tree density and relative elevation, revealing contrasted species-habitat associations in term of abundance, with seasonally inundated bottomlands (24.5% of species) and well-drained plateaus (48.9% of species). We also observed species preferences for environments with or without steep slopes (13.8% and 10.6%, respectively). We observed that closely-related species were frequently associated with different soil habitats in this region (70% of the 14 genera with congeneric species that have a significant association test) suggesting species-habitat associations have arisen multiple times in this tree community. We also tested if species with similar habitat preferences shared functional strategies. We found that seasonally inundated forest specialists tended to have smaller stature (maximum diameter) than species found on plateaus. Our results underline the importance of tree-soil habitat associations in structuring diverse communities at fine spatial scales and suggest that additional studies are needed to disentangle community assembly mechanisms related to dispersal limitation, biotic interactions and environmental filtering from species-habitat associations. Moreover, they provide a framework to generalize across tropical forest sites.
ISSN: 1932-6203

hal-01260289
https://hal.archives-ouvertes.fr/hal-01260289
PRODINRA : 342930
DOI : 10.1371/journal.pone.0141488

Éditeur(s) : HAL CCSD BioMed Central
Résumé : [b]Background[/b][br/] [br/] The Amazonian rainforest is predicted to suffer from ongoing environmental changes. Despite the need to evaluate the impact of such changes on tree genetic diversity, we almost entirely lack genomic resources.[br/] [br/] [b]Results[/b][br/] [br/] In this study, we analysed the transcriptome of four tropical tree species (Carapa guianensis, Eperua falcata, Symphonia globulifera and Virola michelii) with contrasting ecological features, belonging to four widespread botanical families (respectively Meliaceae, Fabaceae, Clusiaceae and Myristicaceae). We sequenced cDNA libraries from three organs (leaves, stems, and roots) using 454 pyrosequencing. We have developed an R and bioperl-based bioinformatic procedure for de novo assembly, gene functional annotation and marker discovery. Mismatch identification takes into account single-base quality values as well as the likelihood of false variants as a function of contig depth and number of sequenced chromosomes. Between 17103 (for Symphonia globulifera) and 23390 (for Eperua falcata) contigs were assembled. Organs varied in the numbers of unigenes they apparently express, with higher number in roots. Patterns of gene expression were similar across species, with metabolism of aromatic compounds standing out as an overrepresented gene function. Transcripts corresponding to several gene functions were found to be over- or underrepresented in each organ. We identified between 4434 (for Symphonia globulifera) and 9076 (for Virola surinamensis) well-supported mismatches. The resulting overall mismatch density was comprised between 0.89 (S. globulifera) and 1.05 (V. surinamensis) mismatches/100 bp in variation-containing contigs.[br/] [br/] [b]Conclusion[/b][br/] [br/] The relative representation of gene functions in the four transcriptomes suggests that secondary metabolism may be particularly important in tropical trees. The differential representation of transcripts among tissues suggests differential gene expression, which opens the way to functional studies in these non-model, ecologically important species. We found substantial amounts of mismatches in the four species. These newly identified putative variants are a first step towards acquiring much needed genomic resources for tropical tree species.
ISSN: 1471-2164

hal-01032439
https://hal.archives-ouvertes.fr/hal-01032439
https://hal.archives-ouvertes.fr/hal-01032439/document
https://hal.archives-ouvertes.fr/hal-01032439/file/art_10-1186_1471-2164-15-238.pdf
DOI : 10.1186/1471-2164-15-238

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The shallow-water bivalve Codakia orbiculata which harbors gill-endosymbiotic sulfur-oxidizing ?-proteobacteria can lose and acquire its endosymbionts throughout its life. Long-term starvation and recolonization experiments led to changes in the organization of cells in the lateral zone of gill filaments. This plasticity is linked to the presence or absence of gill-endosymbionts. Herein, we propose that this reorganization can be explained by three hypotheses: (a) a variation in the number of bacteriocytes and granule cells due to proliferation or apoptosis processes, (b) a variation of the volume of these two cell types without modification in the number, and (c) a combination of both number and cell volume variation. To test these hypotheses, we analyzed cell reorganization in terms of proliferation and apoptosis in adults submitted to starvation and returned to the field using catalyzed reporter deposition fluorescence in situ hybridization, immunohistochemistry, and structural analyses. We observed that cell and tissue reorganization in gills filaments is due to a variation in cell relative abundance that maybe associated with a variation in cell apparent volume and depends on the environment. In fact, bacteriocytes mostly multiply in freshly collected and newly recolonized individuals, and excess bacteriocytes are eliminated in later recolonization stages. We highlight that host tissue regeneration in gill filaments of this symbiotic bivalve can occur by both replication of existing cells and division of undifferentiated cells localized in tissular bridges, which might be a tissue-specific multipotent stem cell zone.
ISSN: 1059-910X

hal-01545915
https://hal.archives-ouvertes.fr/hal-01545915
DOI : 10.1002/jemt.22041

Éditeur(s) : HAL CCSD
Résumé : The forests of French Guiana occupy a central part of the region’s potential resource base, but as yet remain almost completely unexploited. This stems from the forestry industry struggling to valorize wood stocks of a high diversity with an extremely heterogeneous distribution. Indeed, identifying essences for future propagation is an issue of both environmental and socio-economic importance for the region. Bagassa guianensis Aubl. and Cordia alliodora (Ruiz & Pav.) Oken. go against conventional models, in that they display the life history characteristics of rapid growth and wood of high quality, and thus are species of high potential for future plantations. Firstly, our study of growth characteristics reveals a defined strategy half way between long-lived species and pioneer species. It is the variability of basic density of wood which is a key trait allowing for such a development, favoring rapid growth in initial years whilst allowing for tree longevity. Secondly, the analysis of a range of wood properties (shrinkage, modulus of elasticity, durability) in relation to tree growth confirms the technologic quality of the two species. It also enables the emergence of new results like the effect of extractives on wood stability, the control of growth as a result of basic density variation, the evolution of leaf traits throughout tree development or the improvement of trunk maintenance thanks to interlocked grain. The knowledge gained in relation to the developmental traits of these two species, and to the quality of their wood allows for the elaboration of plantation projects and what will be required for their success. The results of this study can orientate future studies on novel species for commercialization in French Guiana.
La ressource forestière en Guyane occupe une place maîtresse au sein de la région et est encore majoritairement préservée, mais la filière bois parvient difficilement à valoriser la biodiversité locale en raison de l’hétérogénéité de la ressource. Identifier les essences à planter demain représente aujourd’hui un enjeu à la fois environnemental, économique et social pour la Guyane. Bagassa guianensis Aubl. et Cordia alliodora (Ruiz & Pav.) Oken. vont à l’encontre des modèles usuels en associant vitesse de croissance et qualité du bois et sont identifiées comme prometteuses pour la plantation. Dans un premier temps, l’étude de la dynamique de croissance des deux espèces révèle un modèle écologique singulier, à la fois pionnières et longévives. La variabilité de l’infradensité dans l’arbre est l’élément clé qui permet un tel développement, favorisant une croissance rapide dans les premières années tout en assurant la longévité de l’individu. Dans une seconde partie, l’analyse d’un large panel de propriétés du bois (retrait, module, durabilité) en lien avec le développement de l’arbre confirme les qualités technologiques des deux espèces et permet l’émergence de résultats novateurs tels que le rôle des extractibles sur le retrait, le contrôle de la croissance grâce aux variations d’infradensité, l’évolution des traits foliaires au cours de l’ontogénie ou encore la mise en place de contrefil dans l’arbre pour assurer son maintien. La connaissance acquise vis-à-vis du mode de développement de ces deux espèces et des facteurs influençant la qualité de leur bois permet aujourd’hui de définir avec plus de précision le cadre d’un projet de plantation et les conditions de son succès. Les résultats de cette étude permettent finalement d’orienter la recherche de nouvelles espèces d’avenir pour la plantation en Guyane.
https://tel.archives-ouvertes.fr/tel-01338281

tel-01338281
https://tel.archives-ouvertes.fr/tel-01338281
https://tel.archives-ouvertes.fr/tel-01338281/document
https://tel.archives-ouvertes.fr/tel-01338281/file/Th%C3%A8se%20Julie%20Bossu.pdf

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
Purpose of review Toxoplasmosis in immunocompromised patients is associated with a high mortality rate. Molecular techniques are important tools to diagnose acute disease in immunocompromised patients, but there are various methods with variable efficiency. Some of them have been validated for the diagnosis of congenital toxoplasmosis, but the impact of their use has not been evaluated in immunocompromised patients. Recent findings Toxoplasmosis is of increasing importance in non-HIV immunocompromised patients. In addition, the picture of disease shows greater severity in South America, both in immunocompetent study participants and in congenitally infected infants. These epidemiological differences could influence the sensitivity of diagnostic methods. This review analyzes recent data on molecular diagnosis and compares them with older ones, in light of progress gained in molecular techniques and of recent epidemiological findings. Most recent studies were conducted in South America and used PCR targeting the B1 gene. PCR on blood could allow diagnosing a significant proportion of patients with ocular toxoplasmosis in Brazil. Summary Quantitative PCR methods with specific probes should be used to improve sensitivity and warrant specificity. Performance of quantitative PCR targeting the repeated 529 bp sequence for the diagnosis of toxoplasmosis in immunocompromised patients needs evaluation in field studies in South America and in western countries.
ISSN: 0951-7375

hal-01390970
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390970
DOI : 10.1097/QCO.0000000000000275
PUBMED : 27191201

Éditeur(s) : HAL CCSD Elsevier
Résumé :  
The within-population spatial structure of genetic diversity is shaped by demographic processes, including historical accidents such as forest perturbations. Information drawn from the analysis of the spatial distribution of genetic diversity is therefore inherently linked to demographic-historical processes that ultimately determine the fate of populations. All adult trees and saplings in a 1.4-ha plot within a mixed Norway spruce (Picea abies [L.] Karst) stand were characterised by means of chloroplast (paternally inherited) markers, and a large sub-sample of these were genotyped at mitochondrial (maternally inherited) molecular markers. These data were used to analyse the spatial distribution of genetic variation and to compare the patterns corresponding to the two marker types. The plot presented non-homogeneous local stem density in the younger cohorts, and the indirect effect of this source of variation on the spatial genetic structure was investigated. Results suggest that (i) spatially limited seed dispersal induced patchiness in genotype distribution, while pollen flow had a homogenising effect; (ii) deviations from random spatial structure were stronger in the demographically most stable portions of the stand, while they were weaker where sudden bursts of regeneration occurred; (iii) spatially overlapping adult and sapling cohorts displayed the same spatial genetic structure (stronger on stable areas, weaker in portions of the stand undergoing events of intense regeneration), which was substantiated by the influence of local demographic processes. Regeneration dynamics as modulated by demography thus influences the distribution of genetic diversity within the stand both in the younger life stages and in the adult population.
ISSN: 0378-1127

hal-01032063
https://hal.archives-ouvertes.fr/hal-01032063
DOI : 10.1016/j.foreco.2008.03.023

Éditeur(s) : HAL CCSD Botanical Society of America
Résumé :  
Tree buckling risk (actual height/critical buckling height) is an important biomechanical trait of plant growth strategies, and one that contributes to species coexistence. To estimate the diversity of this trait among wide samples, a method that minimizes damage to the plants is necessary. On the basis of the rarely used, complete version of Greenhill's model (1881, Proceedings of the Cambridge Philosophical Society 4(2): 65-73), we precisely measured all the necessary parameters on a sample of 236 saplings of 16 species. Then, using sensitivity (variance) analysis, regressions between successive models for risk factors and species ranks and the use of these models on samples of self- and nonself-supporting saplings, we tested different degrees of simplification up to the most simple and widely used formula that assumes that the tree is a cylindrical homogeneous pole. The size factor had the greatest effect on buckling risk, followed by the form factor and the modulus of elasticity of the wood. Therefore, estimates of buckling risk must consider not only the wood properties but especially the form factor. Finally, we proposed a simple but accurate method of assessing tree buckling risk that is applicable to a wide range of samples and that requires mostly nondestructive measurements.
ISSN: 0002-9122

hal-01032059
https://hal.archives-ouvertes.fr/hal-01032059

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : Active mechanisms of re-orientation are necessary to maintain the verticality of tree stems. They are achieved through the production of reaction wood, associated with circumferential variations of three factors related to cambial activity: maturation strain, longitudinal modulus of elasticity (MOE) and eccentric growth. These factors were measured on 17 mature trees from different botanical families and geographical locations. Various patterns of circumferential variation of these factors were identified. A biomechanical analysis based on beam theory was performed to quantify the individual impact of each factor. The main factor of re-orientation is the circumferential variation of maturation strains. However, this factor alone explains only 57% of the re-orientations. Other factors also have an effect through their interaction with maturation strains. Eccentric growth is generally associated with heterogeneity of maturation strains, and has an important complementary role, by increasing the width of wood with high maturation strain. Without this factor, the efficiency of re-orientations would be reduced by 31% for angiosperms and 26% for gymnosperms. In the case of angiosperms, MOE is often larger in tension wood than in normal wood. Without these variations, the efficiency of re-orientations would be reduced by 13%. In the case of gymnosperm trees, MOE of compression wood is lower than that of normal wood, so that re-orientation efficiency would be increased by 24% without this factor of variations.
ISSN: 0931-1890

hal-01031825
https://hal.archives-ouvertes.fr/hal-01031825
DOI : 10.1007/s00468-005-0407-6

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
The shallow-water bivalve Codakia orbiculata which harbors gill-endosymbiotic sulfur-oxidizing g-proteobacteria can lose and acquire its endosymbionts throughout its life. Longterm starvation and recolonization experiments led to changes in the organization of cells in the lateral zone of gill filaments. This plasticity is linked to the presence or absence of gill-endosymbionts. Herein, we propose that this reorganization can be explained by three hypotheses: (a) a variation in the number of bacteriocytes and granule cells due to proliferation or apoptosis processes, (b) a variation of the volume of these two cell types without modification in the number, and (c) a combination of both number and cell volume variation. To test these hypotheses, we analyzed cell reorganization in terms of proliferation and apoptosis in adults submitted to starvation and returned to the field using catalyzed reporter deposition fluorescence in situ hybridization, immunohistochemistry, and structural analyses. We observed that cell and tissue reorganization in gills filaments is due to a variation in cell relative abundance that maybe associated with a variation in cell apparent volume and depends on the environment. In fact, bacteriocytes mostly multiply in freshly collected and newly recolonized individuals, and excess bacteriocytes are eliminated in later recolonization stages. We highlight that host tissue regeneration in gill filaments of this symbiotic bivalve can occur by both replication of existing cells and division of undifferentiated cells localized in tissular bridges, which might be a tissue-specific multipotent stem cell zone.
Microscopy Research and Technique

hal-00743036
https://hal-uag.archives-ouvertes.fr/hal-00743036

Résumé : L'espèce Ludwigia leptocarpa (Onagracées) présente dans le paysage antillais est méconnue par la population locale. Pourtant, au Nigéria, ses feuilles sont préconisées pour traiter les rhumatismes, la dysenterie et pour leurs vertus laxative et vermifuge. L'absence de données phytochimiques sur cette plante justifie le choix de notre étude. Un criblage phytochimique réalisé sur les extraits bmts de feuilles, de fruits et de tiges a mis en évidence la présence de terpénoïdes dans les extraits hexane et dichlorométhane et des acides phénoliques et flavonoïdes dans les extraits méthanol et eau. L'évaluation du potentiel biologique des extraits apolaires des feuilles a révélé des activités antibiotique (E. coli et S. aureus) et anti-inflammatoire intéressantes. Les extraits plus polaires présentent des potentiels antioxydant, antibiotique (E. coli et S. aureus), antifongique (c. albicans et C. tropica/is), et antiviral (HSV-l) significatifs. L'étude des fractions volatiles de feuilles, de tiges et des parties aériennes par microextraction en phase solide a conduit à l'identification de 54 composés. Les analyses GC/MS menées sur les extraits apolaires de feuilles et de fruits ont mis en évidence la présence d'acides gras, de triterpènes et de stéroïdes. Le fractionnement et la purification des extraits polaires de feuilles ont mené à l'isolation et l'identification de 8 composés phénoliques parmi lesquels 1 acide polyhydroxylé, 3 acides phénoliques, 2 flavonoïdes et 2 tanins galliques. L'apport de nouvelles données scientifiques sur 1. leptocarpa contribue à la valorisation de cette matière végétale locale et laisse entrevoir des perspectives de recherche et d'application
Ludwigia Leptocarpa species (Onagraceae) is present in Caribean landscape, it remains unknown by French West Indies population. However, in Nigeria the leaves are recommended to treat rheumatism, dysentery and as laxative or deworming. The lack of data phytochemical on this plant justifies our study choice. A phytochemical screening carried out on crude extracts from leaves, fruits and stems highlighted terpenoids presence in hexane and dichloromethane crude extracts and phenolic compounds (phenolic acids and flavonoids) in methanol and water cmde extracts. The biological potentials evaluation of apolar extracts showed interesting antibiotic (E. coli and S. aureus) and inflammatory activities. Polar extracts present antioxidant, antibiotic (E. coli and S. aureus), antifungal (C albicans and C tropicalis), and viral (HSV-l) potential. Volatile fraction study of lcave, stems and aerial parts by Solid Phase MicroExtraction (SPME) 100 to theidentification of 54 compounds. GCIMS analysis conducted on leaves and fruits apolar extracts underlined the presence of fatty acid, triterpenes and steroids. Leaves polar extract fractionnation and purification resulted to isolation and identification of 8 phenolic componuds including 1 polyhydroxylated acid, 3 phenolic acids, 2 flavonoids and 2 gallic tannins. The addition of new scientific data on L. /eptocarpa contributes to the enhancement of this local plant rnaterial and suggests many opportunities for research and application

http://www.theses.fr/2013AGUY0676/document

Éditeur(s) : HAL CCSD
Résumé : The forests of French Guiana occupy a central part of the region’s potential resource base, but as yet remain almost completely unexploited. This stems from the forestry industry struggling to valorize wood stocks of a high diversity with an extremely heterogeneous distribution. Indeed, identifying essences for future propagation is an issue of both environmental and socio-economic importance for the region. Bagassa guianensis Aubl. and Cordia alliodora (Ruiz & Pav.) Oken. go against conventional models, in that they display the life history characteristics of rapid growth and wood of high quality, and thus are species of high potential for future plantations. Firstly, our study of growth characteristics reveals a defined strategy half way between long-lived species and pioneer species. It is the variability of basic density of wood which is a key trait allowing for such a development, favoring rapid growth in initial years whilst allowing for tree longevity. Secondly, the analysis of a range of wood properties (shrinkage, modulus of elasticity, durability) in relation to tree growth confirms the technologic quality of the two species. It also enables the emergence of new results like the effect of extractives on wood stability, the control of growth as a result of basic density variation, the evolution of leaf traits throughout tree development or the improvement of trunk maintenance thanks to interlocked grain. The knowledge gained in relation to the developmental traits of these two species, and to the quality of their wood allows for the elaboration of plantation projects and what will be required for their success. The results of this study can orientate future studies on novel species for commercialization in French Guiana.
La ressource forestière en Guyane occupe une place maîtresse au sein de la région et est encore majoritairement préservée, mais la filière bois parvient difficilement à valoriser la biodiversité locale en raison de l’hétérogénéité de la ressource. Identifier les essences à planter demain représente aujourd’hui un enjeu à la fois environnemental, économique et social pour la Guyane. Bagassa guianensis Aubl. et Cordia alliodora (Ruiz & Pav.) Oken. vont à l’encontre des modèles usuels en associant vitesse de croissance et qualité du bois et sont identifiées comme prometteuses pour la plantation. Dans un premier temps, l’étude de la dynamique de croissance des deux espèces révèle un modèle écologique singulier, à la fois pionnières et longévives. La variabilité de l’infradensité dans l’arbre est l’élément clé qui permet un tel développement, favorisant une croissance rapide dans les premières années tout en assurant la longévité de l’individu. Dans une seconde partie, l’analyse d’un large panel de propriétés du bois (retrait, module, durabilité) en lien avec le développement de l’arbre confirme les qualités technologiques des deux espèces et permet l’émergence de résultats novateurs tels que le rôle des extractibles sur le retrait, le contrôle de la croissance grâce aux variations d’infradensité, l’évolution des traits foliaires au cours de l’ontogénie ou encore la mise en place de contrefil dans l’arbre pour assurer son maintien. La connaissance acquise vis-à-vis du mode de développement de ces deux espèces et des facteurs influençant la qualité de leur bois permet aujourd’hui de définir avec plus de précision le cadre d’un projet de plantation et les conditions de son succès. Les résultats de cette étude permettent finalement d’orienter la recherche de nouvelles espèces d’avenir pour la plantation en Guyane.
https://tel.archives-ouvertes.fr/tel-01430243

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2015YANE0002
tel-01430243
https://tel.archives-ouvertes.fr/tel-01430243
https://tel.archives-ouvertes.fr/tel-01430243/document
https://tel.archives-ouvertes.fr/tel-01430243/file/julie-bossu.pdf

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Defining homologous genes is important in many evolutionary studies but raises obvious issues. Some of these issues are conceptual and stem from our assumptions of how a gene evolves, others are practical, and depend on the algorithmic decisions implemented in existing software. Therefore, to make progress in the study of homology, both ontological and epistemological questions must be considered. In particular, defining homologous genes cannot be solely addressed under the classic assumptions of strong tree thinking, according to which genes evolve in a strictly tree-like fashion of vertical descent and divergence and the problems of homology detection are primarily methodological. Gene homology could also be considered under a different perspective where genes evolve as ``public goods,'' subjected to various introgressive processes. In this latter case, defining homologous genes becomes a matter of designing models suited to the actual complexity of the data and how such complexity arises, rather than trying to fit genetic data to some a priori tree-like evolutionary model, a practice that inevitably results in the loss of much information. Here we show how important aspects of the problems raised by homology detection methods can be overcome when even more fundamental roots of these problems are addressed by analyzing public goods thinking evolutionary processes through which genes have frequently originated. This kind of thinking acknowledges distinct types of homologs, characterized by distinct patterns, in phylogenetic and nonphylogenetic unrooted or multirooted networks. In addition, we define `` family resemblances'' to include genes that are related through intermediate relatives, thereby placing notions of homology in the broader context of evolutionary relationships. We conclude by presenting some payoffs of adopting such a pluralistic account of homology and family relationship, which expands the scope of evolutionary analyses beyond the traditional, yet relatively narrow focus allowed by a strong tree-thinking view on gene evolution.
ISSN: 0737-4038

hal-01544794
https://hal.archives-ouvertes.fr/hal-01544794
DOI : 10.1093/molbev/mst228

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : Key message Molecular markers were used for paternity recovery in a maritime pine (Pinus pinasterAit.) polycross trial, facilitating forward selection. Different breeding strategies for seed orchard establishment were evaluated by comparing genetic gains and diversity. This work opens up new perspectives in maritime pine breeding.Context Polycross mating designs are widely used in forest tree breeding to evaluate parental breeding values for backward selection. Alternatively, polycross progeny trials may be used to select the best trees on the basis of individual breeding values and molecular pedigree analysis.Aims This study aimed to test such a forward selection strategy for the maritime pine breeding program.Methods In a maritime pine polycross trial, progeny with higher breeding values for growth and stem straightness was first preselected with or without relatedness constraints. After paternity recovery, the preselected trees were ranked on the basis of their breeding values, estimated from the recovered full pedigree. Finally, the best candidates were selected with three different strategies (forward, backward, mixed) and three levels of coancestry constraints to establish a virtual clonal seed orchard.Results Complete pedigrees were successfully recovered for most of the preselected trees. There was no major difference in expected genetic gains between the two preselection strategies which differed for relatedness constraints. Genetic gains were slightly higher for forward selection than for classical backward selection.Conclusion This seminal study opens up new perspectives for using forward selection within the French maritime pine breeding program.
ISSN: 1932-6203

hal-01601804
https://hal.archives-ouvertes.fr/hal-01601804
DOI : 10.1371/journal.pone.0049990
PRODINRA : 391860

Résumé : La banane plantain (Musa paradisiaca) est l’aliment de base de millions de personnes dans le monde et sa culture génère des revenus permanents pour un grand nombre d’agriculteurs, dans des plantations de taille petite ou moyenne. Comme dans d’autres régions tropicales, la culture du plantain en Guadeloupe et en Haïti est soumise à de fortes contraintes parasitaires aussi bien telluriques (i.e. du sol : nématodes phytoparasites et charançon du bananier) qu’aériennes (Cercosporiose noire notamment). Les moyens de lutte conventionnels reposent sur l’usage de produits de synthèse dont les effets néfastes sur l’environnement (sols, eaux, animaux) comme sur la santé humaine ne sont plus supportables. Il est donc urgent de réfléchir à des solutions agro-écologiques permettant de rétablir les équilibres biologiques, de maintenir une bonne qualité des sols et une production optimale dans les systèmes de culture plantains. C’est le but de ce travail de thèse qui couple la réalisation d’un diagnostic agro-écologique dans des parcelles paysannes, et le test d’alternatives agro-écologiques en milieu semi-contrôlé. Pour ce faire, une typologie des systèmes de culture plantains a été réalisée à l’issue d’une enquête agro-environnementale dans les deux zones d’étude. Elle a permis de sélectionner 23 parcelles en Guadeloupe et 12 en Haïti dans lesquelles un diagnostic agro-écologique a été conduit. Sur la base de ce diagnostic et de la recherche d’alternatives agro-écologiques à l’usage des produits chimiques, une expérimentation au champ a été mise en place en Guadeloupe en station de recherche, permettant le test de trois pratiques culturales innovantes pour le plantain (seules et combinées), à savoir : i) l’introduction d’une plante de service Paspalum notatum pour la gestion des adventices et la réduction de l’utilisation d’herbicides ; ii) l’apport de vermicompost pour le contrôle des nématodes phytoparasites inféodés au bananier plantain et la fertilisation de celui-ci ; iii) l’utilisation de plants sains PIF (Plants Issus de Fragments de tiges) indemnes de nématodes et de larves de charançon du bananier. La typologie des systèmes de culture plantains révèle que : i) en Guadeloupe les précédents : jachère, ananas et banane plantain sont prédominants avec un niveau d’intensification faible (apports d’intrants chimiques faibles et peu fréquents) ou élevé (apports d’intrants chimiques élevés et plus fréquents) ; ii) en Haïti, les précédents : jachère, banane plantain et manioc prédominent avec un niveau d’intensification faible ou nul (apport d’intrants inexistant). Les résultats du diagnostic agro-écologique montrent que, i) lorsque le niveau d’intensification est faible, les bananeraies plantains pérennes et le précédent-ananas permettent de maintenir une bonne qualité du sol et une bonne régulation des parasites telluriques ; ii) lorsque le niveau d’intensification est fort, les populations d’ingénieurs du sol diminuent drastiquement, alors que le cortège parasitaire tellurique augmente sans que cela n’affecte l’obtention de bons niveaux de rendement instantannés (parcelles précédées d’ananas ou d’une jachère principalement) ; iii) en absence totale de fertilisation, il résulte une diminution de l’activité biologique du sol mais aussi du rendement du plantain, exacerbé par le choix des précédents-culturaux (manioc ou banane plantain) en lien avec les contraintes pédoclimatiques et la maladie des raies noires (Cercosporiose noire) causée par Mycosphaerella fijiensis, notamment au sein des parcelles Haïtiennes ; iv) la succession plantain/plantain est la plus pénalisante vis-à-vis de la culture du plantain, car quelque soit le niveau d’intensification, le rendement reste relativement faible, en lien avec une dégradation de l’état sanitaire, comparativement aux autres précédents.
Plantain (Musa paradisiaca) is the staple food of millions of people worldwide and its cropping generates ongoing revenues for many farmers who are planting small or medium size areas. As in other tropical regions, plantain cultivation in Guadeloupe and Haiti is under heavy parasitic constraints terrestrial (plant-parasitic nematodes and banana weevil) as well as aerial (black Sigatoka in particular). Conventional means of control based on the use of synthetic products which adverse effects on the environment (soil, water, animals ...) as on human health are not bearable any more. It is thus urgent to think about agroecological solutions allowing to restore the biological balances, to maintain good soil quality and optimal plantain cropping systems.This is the ultimate goal of this thesis which couples the realization of an agroecological diagnosis in peasants’ plots, and the test of agroecological alternatives in semi-controlled conditions. To do this, a typology of plantain cropping systems was carried out from an agrienvironmental survey in the two study areas. It allowed to select 23 plots in Guadeloupe and 12 in Haiti in which an agroecological diagnosis was implemented. Based on this diagnosis and the research of agroecological alternatives to the use of chemicals, a field experiment was set up in Guadeloupe, in an experimental station allowing the test of three innovative practices for plantain cultivation (alone and combined), namely : i) the introduction of a cover-crop Paspalum notatum for weed control while reducing the use of herbicides ; ii) the input of worms’ compost to control plant-parasitic nematodes specific to plantain and to fertilize it ; iii) the use of healthy “PIF” plants (plants issued from stem fragments) free from telluric pests (nematodes and weevil’s larvaes).The typology of plantains cropping systems shows: i) in Guadeloupe the previous crops are: fallow pineapple and plantain predominate with a low level of intensification (low and infrequent chemical inputs) or high (high and frequent chemical inputs); ii) in Haiti, the previous crops are: fallow, plantain and cassava predominate with a low level or no intensification at all (no inputs). The results of the agroecological analysis show that : i) when the level of intensification is low, perennial plantain and pineapple as previous crops help maintaining a good soil quality and a good regulation of the telluric pests ; ii) when the level of intensification is strong, the soil engineers drastically reduce, while the density of telluric parasites increases without affecting good levels of instantaneous yields (plots where the previous crop is pineapple or mostly fallow) ; iii) when the fertilization is totally missing, it decreases the biological activity of the soil furthermore the plantain yields, exacerbated by the choice of the previous crop (cassava or plantain), in connection with soils and climate constraints and the black Sigatoka caused by Mycosphaerella fijiensis, especially in the Haitian plots ; iv) the crop succession plantain/plantain is the most critical regarding the plantain’s cropping, because whatever the level of intensification, the yields remain relatively low in connection with a degradation of the health state, compared to other previous crops.The driving of an experiment in a research station shows that on the scale of one year, the three tested innovative practices allow maintaining a good soils quality. Healthy plants "PIF" have a better health state (absence of plant parasitic nematodes in the roots) which helps a significant increase of the yields. Cover-crop P. notatum helps the weeds and the soil pests control and favors the improvement of soil biological activity and plantain yields. Worms’ compost contributes to the maintenance of a better soils quality while allowing the regulation of the populations of plant-parasitic nematodes of the plantains.

http://www.theses.fr/2014AGUY0758/document