Éditeur(s) : HAL CCSD SPRINGER
Résumé : International audience
A key question in evolutionary biology is the reproducibility of adaptation. This question can now be quantitatively analyzed using experimental evolution coupled to whole genome sequencing (WGS). With complete sequence data, one can assess convergence among replicate populations. In turn, convergence reflects the action of natural selection and also the breadth of the field of possible adaptive solutions. That is, it provides insight into how many genetic solutions or adaptive paths may lead to adaptation in a given environment. Convergence is both a property of an adaptive landscape and, reciprocally, a tool to study that landscape. In this chapter we present the links between convergence and the properties of adaptive landscapes with respect to two types of microbial experimental evolution. The first tries to reconstruct a full adaptive landscape using a handful of carefully identified mutations (the reductionist approach), while the second uses WGS of replicate experiments to infer properties of the adaptive landscape. Reductionist approaches have highlighted the importance of epistasis in shaping the adaptive landscape, but have also uncovered a wide diversity of landscape architectures. The WGS approach has uncovered a very high diversity of beneficial mutations that affect a limited set of genes or functions and also suggests some shortcomings of the reductionist approach. We conclude that convergence may be better defined at an integrated level, such as the genic level or even at a phenotypic level, and that integrated mechanistic models derived from systems biology may offer an interesting perspective for the analysis of convergence at all levels.
ECOLOGICAL GENOMICS: ECOLOGY AND THE EVOLUTION OF GENES AND GENOMES

ISBN : 978-94-007-7347-9; 978-94-007-7346-2
hal-01544757
https://hal.archives-ouvertes.fr/hal-01544757
DOI : 10.1007/978-94-007-7347-9__11

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Ameloblastin (AMBN) is a phosphorylated, proline/glutamine-rich protein secreted during enamel formation. Previous studies have revealed that this enamel matrix protein was present early in vertebrate evolution and certainly plays important roles during enamel formation although its precise functions remain unclear. We performed evolutionary analyses of AMBN in order to (i) identify residues and motifs important for the protein function, (ii) predict mutations responsible for genetic diseases, and (iii) understand its molecular evolution in mammals. Results: In silico searches retrieved 56 complete sequences in public databases that were aligned and analyzed computationally. We showed that AMBN is globally evolving under moderate purifying selection in mammals and contains a strong phylogenetic signal. In addition, our analyses revealed codons evolving under significant positive selection. Evidence for positive selection acting on AMBN was observed in catarrhine primates and the aye-aye. We also found that (i) an additional translation initiation site was recruited in the ancestral placental AMBN, (ii) a short exon was duplicated several times in various species including catarrhine primates, and (iii) several polyadenylation sites are present. Conclusions: AMBN possesses many positions, which have been subjected to strong selective pressure for 200 million years. These positions correspond to several cleavage sites and hydroxylated, O-glycosylated, and phosphorylated residues. We predict that these conserved positions would be potentially responsible for enamel disorder if substituted. Some motifs that were previously identified as potentially important functionally were confirmed, and we found two, highly conserved, new motifs, the function of which should be tested in the near future. This study illustrates the power of evolutionary analyses for characterizing the functional constraints acting on proteins with yet uncharacterized structure.
ISSN: 1471-2148

Droits : http://creativecommons.org/licenses/by/
hal-01212816
http://hal.upmc.fr/hal-01212816
http://hal.upmc.fr/hal-01212816/document
http://hal.upmc.fr/hal-01212816/file/s12862-015-0431-0.pdf
DOI : 10.1186/s12862-015-0431-0

Éditeur(s) : HAL CCSD
Résumé : International audience
In two swordtail species of the genus Xiphophorus, the onset of puberty has been shown to be modulated at the P locus by sequence polymorphism and gene copy-number variation affecting the type 4 melanocortin hormone receptor Mc4r. The system works through the interaction of two allelic types, one encoding wild type and the other dominant-negative receptors. We have analyzed the structure and evolution of the P locus in the platyfish Xiphophorus maculatus, where as many as nine alleles of P determining the onset of sexual maturity in males and females, fecundity in females, and adult size in males are located on both the X and Y chromosomes in a region linked to the master sex-determining locus. In this species, mc4r has been amplified to up to 10 copies on both the X and Y chromosomes through recent large serial duplications. Subsequently, mc4r paralogues have diverged considerably into many different subtypes. Certain copies have acquired new untranslated regions through genomic rearrangements, and transposable element insertions and other mutations have accumulated in promoter regions, possibly explaining observed deviations from the classical mc4r transcriptional pattern. In the mc4r-coding sequence, in-frame insertions and deletions as well as nonsense and missense mutations have generated a high diversity of Mc4r-predicted proteins. Most of these variants are expressed in embryos, adults, and/or tumors. Functional receptor characterization demonstrated major divergence in pharmacological behavior for Mc4r receptors encoded by different copies of platyfish mc4r, with differences in constitutive activity as well as binding and stimulation by hormones. The high degree of allelic and copy-number variation observed between individuals can explain the high level of polymorphism for sexual maturation, fecundity, and body size in the platyfish: multiple combinations of Mc4r variants with different biochemical properties might interact to modulate the melanocortin signaling that regulates the hypothalamus-pituitary-gonadal axis.
GENETICS

hal-01544912
https://hal.archives-ouvertes.fr/hal-01544912
DOI : 10.1534/genetics.113.155952

Éditeur(s) : HAL CCSD Springer Verlag/EDP Sciences
Résumé : Four populations of Norway spruce (Picea abies Karst.) were screened using nine nuclear microsatellite markers (three trinucleotides and six dinucleotides) and four chloroplast markers (all mononucleotides). Marker classes were compared for their variability, mutation rate and ability to detect differentiation between stands. Dinucleotide markers proved to be the most variable group and chloroplast stretches the least variable, with differences in mutation rate between the former and the latter spanning over two orders of magnitude. Variability correlated to the number of repeats but not to the absolute length of the microsatellite region. The different marker classes were combined with two different measures of genetic distance in order to investigate the performance of markers and evolutionary models for the study of genetic variation in natural populations of Norway spruce. Weir and Cockeram's F$_{rm ST}$ generally performed better in this clear-cut, four-population model study. Chloroplast haplotypes turned out to be the most sensitive marker system, being able to differentiate populations and to detect differences in genetic variability between sub-regions.
ISSN: 1286-4560

hal-01032048
https://hal.archives-ouvertes.fr/hal-01032048
DOI : 10.1051/forest:2006029

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (B[a]P), are widely distributed environmental contaminants exerting toxic effects such as genotoxicity and carcinogenicity, mainly associated with aryl hydrocarbon receptor (AhR) activation and the subsequent induction of cytochromes P-450 (CYP) 1-metabolizing enzymes. We previously reported an up-regulation of AhR expression and activity in primary cultures of human T lymphocyte by a physiological activation. Despite the suggested link between exposure to PAHs and the risk of lymphoma, the potential of activated human T lymphocytes to metabolize AhR exogenous ligands such as B[a]P, and produce DNA damage has not been investigated. In the present study, we characterized the genotoxic response of primary activated T lymphocytes to B[a] P. We demonstrated that, following T lymphocyte activation, B[a]P treatment triggers a marked increase in CYP1 expression and activity generating, upon metabolic activation, DNA adducts and double-strand breaks (DSBs) after a 48-h treatment. At this time point, B[a]P also induces a DNA damage response with ataxia telangiectasia mutated kinase activation, thus producing a p53-dependent response and T lymphocyte survival. B[a]P activates DSB repair by mobilizing homologous recombination machinery but also induces gene mutations in activated human T lymphocytes which could consequently drive a cancer process. In conclusion, primary cultures of activated human T lymphocytes represent a good model for studying genotoxic effects of environmental contaminants such as PAHs, and predicting human health issues.
ISSN: 0006-2952

hal-01560145
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560145
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560145/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560145/file/Liamin%20et%20al.%20-%20Benzo%5Ba%5Dpyrene-induced%20DNA%20damage%20associated%20with%20.pdf
DOI : 10.1016/j.bcp.2017.04.025

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
Zidi-Yahiaoui N, Callebaut I, Genetet S, Le Van Kim C, Cartron JP, Colin Y, Ripoche P, Mouro-Chanteloup I. Functional analysis of human RhCG: comparison with E. coli ammonium transporter reveals similarities in the pore and differences in the vestibule. Am J Physiol Cell Physiol 297: C537–C547, 2009. First published June 24, 2009; doi:10.1152/ajpcell.00137.2009.—Rh glycoproteins are members of the ammonium transporter (Amt)/methylamine permease (Mep)/Rh family facilitating movement of NH3 across plasma membranes. Homology models constructed on the basis of the experimental structures of Escherichia coli AmtB and Nitrosomonas europaea Rh50 indicated a channel structure for human RhA (RhAG), RhB (RhBG), and RhC (RhCG) glycoproteins in which external and internal vestibules are linked by a pore containing two strictly conserved histidines. The pore entry is constricted by two highly conserved phenylalanines, “twin-Phe.” In this study, RhCG function was investigated by stopped-flow spectrofluorometry measuring kinetic pH variations in HEK293E cells in the presence of an ammonium gradient. The apparent unitary NH3 permeability of RhCG was determined and was found to be close to that of AmtB. With a site-directed mutagenesis approach, critical residues involved in Rh NH3 channel activity were highlighted. In the external vestibule, the importance of both the charge and the conformation of the conserved aspartic acid was shown. In contrast to AmtB, individual mutations of each phenylalanine of the twin-Phe impaired the function while the removal of both resulted in recovery of the transport activity. The impact of the mutations suggests that, although having a common function and a similar channel structure, bacterial AmtB and human Rh vary in several aspects of the NH3 transport mechanisms.
ISSN: 0363-6143

hal-00417492
https://hal.archives-ouvertes.fr/hal-00417492
DOI : 10.1152/ajpcell.00137.2009.

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Estrogen receptor alpha (ERα) is generally considered to be a good prognostic marker because almost 70% of ERα-positive tumors respond to anti-hormone therapies. Unfortunately, during cancer progression, mammary tumors can escape from estrogen control, resulting in resistance to treatment. In this study, we demonstrate that activation of the actin/megakaryoblastic leukemia 1 (MKL1) signaling pathway promotes the hormonal escape of estrogen-sensitive breast cancer cell lines. The actin/MKL1 signaling pathway is silenced in differentiated ERα-positive breast cancer MCF-7 and T47D cell lines and active in ERα-negative HMT-3522 T4-2 and MDA-MB-231 breast cancer cells, which have undergone epithelial-mesenchymal transition. We showed that MKL1 activation in MCF-7 cells, either by modulating actin dynamics or using MKL1 mutants, down-regulates ERα expression and abolishes E2-dependent cell growth. Interestingly, the constitutively active form of MKL1 represses PR and HER2 expression in these cells and increases the expression of HB-EGF, TGFβ, and amphiregulin growth factors in an E2-independent manner. The resulting expression profile (ER-, PR-, HER2-) typically corresponds to the triple-negative breast cancer expression profile.
ISSN: 0303-7207

hal-01061363
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061363
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061363/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01061363/file/Activationsur the_MKL1actin_Kerdivel_et_al._text_Figures.pdf
DOI : 10.1016/j.mce.2014.03.009

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : In recent years, the use of fads in Martinique has evolved from an experimental stage to a productive one. This transformation results in the pitching up of operational device park by the regional fishery committee. Nevertheless, this technical change in fishing practices is not implemented in a homogeneous way all around the island; some differences exist especially between the Atlantic and the Caribbean coasts. From surveys carried out during an annual cycle on two main landing sites, this study describes the pelagic fishing activity around fads and in the open sea. A comparison of fishing strategies adopted in different places as well as an analysis of the results obtained by the fishing units in terms of species (size of catches and daily yields) allow to describe the progress made in the implementation adoption of this new fishing practice. For several fishermen, fishing around fads has become an exclusive job, practised all year round. For others, fads is used in addition to traditional fishing activity on flotsam, "Miquelon" fishing and keeps a seasonal character.Original Abstract: Au cours des recentes annees, l'utilisation des DCP en Martinique est passee du stade experimental au stade operationnel. Cette mutation se traduit par la prise en charge du parc de dispositifs operationnels par le comite regional des peches. Toutefois, l'adoption de cette nouvelle technique d'exploitation ne s'effectue pas de facon homogene dans toutes les communes de l'ile, des differences existent notamment entre les cotes Atlantique et Caraibe. A partir d'enquetes effectuees pendant un cycle annuel, sur deux principaux points de debarquement, cette etude decrit l'activite de peche des poissons pelagiques avec ou sans DCP. Une comparaison des strategies de peche developpees dans les differents sites ainsi qu'une analyse des resultats obtenus par les unites de peche en termes d'especes, de taille des captures, de rendement journalier permettent de decrire la progression differenciee de l'adoption de cette nouvelle activite halieutique. Pour certains pecheurs, la peche sous DCP est devenue un metier exclusif, pratique tout au long de l'annee. Pour d'autres, le DCP est utilise en complement de l'activite traditionnelle de peche sous bois derivants, " peche a Miquelon ", et conserve un caractere saisonnier.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15298/12657.pdf
http://archimer.ifremer.fr/doc/00042/15298/

Éditeur(s) : HAL CCSD
Résumé : Hepatitis C virus (HCV) is one of the leading causes of hepatocellular carcinoma. Therapeutic treatment against the virus has been recently improved by a tritherapy including pegylated interferon, ribavirin and antiviral protease. Nevertheless, the importance of side effects and the emergence of resistant mutants require the development of new antivirals. In this context, our team has recently discovered that Type III phosphatidylinositol 4-kinases (PI4KIIIα and PI4KIIIβ) are essential for the propagation of the virus in a human hepatic cell line at the entry and replication steps. To further characterize these potential therapeutic targets, we investigate the implication of these kinases during the HCV entry, using primary human hepatocytes, a model closer to the in vivo conditions. Two lines of research were developed: Verification of the importance of the kinase activity of PI4KIIIs during HCV entry in primary human hepatocytes, through a chemical approach; Validation of the involvement of these kinases and their enzymatic activity during viral entry through a genetic approach based on RNA interference and phenotype rescue. In parallel, we studied the expression of PI4KIIIα in liver diseases. Our results suggest the involvement of PI4KIIIα in HCV entry; the involvement of PI4KIIIIβ needs to be confirmed. The analysis of PI4KIIIα expression in hepatocellular carcinoma led us to propose this kinase as a new molecular marker, which could improve the already established prognosis models and could lead to the development of new therapeutic approaches.
Le virus de l’hépatite C (VHC) est l’un des principaux facteurs étiologiques du carcinome hépatocellulaire. Le traitement des hépatites virales C a été récemment amélioré grâce à une trithérapie (interféron, ribavirine et anti-protéase virale). Néanmoins l’importance des effets secondaires et l’émergence de mutants résistants nécessitent de découvrir de nouveaux antiviraux. Dans ce contexte, notre équipe a récemment découvert que les phosphatidylinositol 4-kinases de type III (PI4KIIIα et PI4KIIIβ) étaient indispensables à la propagation du virus dans une lignée hépatique humaine, et ce, à 2 étapes de son cycle biologique : l’entrée et la réplication. L’objectif du présent travail était de poursuivre la validation de ces nouvelles cibles thérapeutiques potentielles, en approfondissant nos connaissances sur la dépendance du virus à l’égard de ces kinases au cours de son entrée. Pour cela, nous avons utilisé le modèle des hépatocytes humains primaires, système in vitro plus proche du contexte physiologique que les modèles utilisés jusqu’à présent et qui étaient basés sur l’exploitation de lignées. Deux axes ont été développés : Vérification de l’importance de l’activité kinase des PI4KIIIs au cours de l’entrée du VHC dans les hépatocytes humains primaires, à travers une approche chimique ; Validation de l’implication de ces kinases et de leur activité enzymatique au cours de l’entrée virale grâce à une approche génétique basée sur l’ARN interférence et la restauration de phénotype. En parallèle, nous avons étudié l’expression de PI4KIIIα au cours de pathologies hépatiques. Nos résultats suggèrent une implication de PI4KIIIα au cours de l’entrée du VHC dans les hépatocytes humains primaires, mais restent à confirmer quant à l’implication de PI4KIIIβ. Par ailleurs, l’analyse de l’expression de PI4KIIIα dans le carcinome hépatocellulaire (CHC) conduit à proposer cette kinase comme un nouveau marqueur moléculaire, qui pourrait améliorer les modèles de pronostic déjà établis et pourrait conduire au développement de nouvelles approches thérapeutiques pour les patients atteints d’un CHC, quelque soit l’étiologie.
https://tel.archives-ouvertes.fr/tel-00950335

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2013REN1B006
tel-00950335
https://tel.archives-ouvertes.fr/tel-00950335
https://tel.archives-ouvertes.fr/tel-00950335/document
https://tel.archives-ouvertes.fr/tel-00950335/file/ILBOUDOAdeodat.pdf

Résumé : Aux Antilles, la faible fréquence des coronaropathies pourrait être liée à des facteurs génétiques, ethniques et/ou une distribution spécifique des facteurs de risque cardio-vasculaire (FDRCV). Les propriétés l'ANP (Atrial Natriuretic Peptide) et l'influence de certains polymorphismes de son gène, fréquents dans les populations d'ascendance Africaine pourraient également être en cause. L'analyse des dossiers de 638 patients coronariens consécutifs a mis en évidence une forte prévalence de l'hypertension artérielle et du diabète dans cette population et révélé des différences significatives dans la prévalence des FDRCV entre Afro-Caribéens (AC), Caucasiens et descendants de migrants Indiens. L'analyse angiographique de 420 patients AC a révélé que le diabète était le FDRCV le plus fortement lié à la sévérité des lésions coronaires alors l'obésité apparaissait comme un facteur protecteur. La transfection in vitro, de cellules musculaires lisses artérielles par une construction adénovirale porteuse du gène de l'ANP a entrainé une inhibition de la prolifération et de la migration cellulaire de 31 % et 25% respectivement. In vivo, cet effet s'est traduit par une réduction de 25% de la formation néotimale et de 28% du rapport intima/média dans un modèle d'hyperplasie de carotide de rat. Dans étude transversale incluant 210 sujets diabétiques AC nous avons mis en évidence une association entre le polymorphisme rs5065 (22381>C) du gène de l'ANP et l'existence d'une coronaropatbie. Dans cette étude, l'odds ratio de la présence d'une coronaropathie chez les porteurs de l'allèle mineur de cette mutation (TC/CC) était de 0,50 (0,26- 0,96; P = 0,038).
In Guadeloupe and Martinique, the low frequency ofcoronary artery disease (CAD) could be related to genetic or ethniefactors and/or specifie distribution ofcardiovascular risk factors (CRFs). Atrial Natriuretic Peptide (ANP) properties and influence of sorne of ANP gene polymorphism that are frequent in populations of African descent could also be involved in CAD occurrence. We retrospectively studied 638 consecutive patients with documented CAO and found a high prevalence of hypertension and diabetes in this population. This study also demonstrated significant differences in the prevalence of CRFs between Afro-Caribbean (AC), Caucasians and Indians migrants. The angiographie analysis of420 patients revealed that in AC, diabetes emerged as the strongest CFR related to the severity ofcoronary lesions while obesity appeared as a protective factor. After transfection by an adenoviral construct carrying the ANP gene, vascular smooth muscle cell proliferation stimulated by 10% fetal calf serum was reduced by 31% and migration by 25%. In vivo, in rat carotid artery model ofvascular injury, neointima formation and intima/media ratio was reduced by 25% and 28% respectively. In a cross-sectional study inc1uding 210 AC diabetics we found an association between rs5065 (22381> C) polymorpbism and the presence of coronary artery disease suggesting that the minor allele could have a protective effect against CAD. The odds ratio for the presence ofCAD in carriers ofthe minor allele ofthis mutation (TC / CC) was 0.50 (0.26-0.96, P = 0.038).

http://www.theses.fr/2012AGUY0553/document

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : À travers l’étude du dispositif de la formation professionnelle en Martinique, étendue à la Guadeloupe et à la Guyane française, l’article analyse la spécificité des dispositifs de formation sur ces territoires français des Amériques par comparaison à la France métropolitaine puis souligne les mutations qui se produisent dans les dispositifs de formation à distance de ces territoires sous l’effet du développement des nouvelles technologies de l’information. L’intérêt est d’engager une réflexion sur la relation problématique entre emploi et formation en intégrant le rôle du politique et du contexte social et culturel
Through the study of the approaches to professional training in Martinique, Guadeloupe and French Guiana, this article compares how training in these territories of French America compares to training in France and then highlights the changes that have occurred as a result of the introduction and development of the new information technologies into distance education programmes in those territories. Our purpose is to initiate a reflection on the need to examine the relationship between employment and training by integrating the role of politics and the social and cultural context
Martinique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.488
http://journals.openedition.org/etudescaribeennes/488

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Introduction Bien que les îles de la Caraïbe présentent des caractéristiques historiques, culturelles et économiques similaires, leur urbanisation et leur évolution démographique révèlent des discontinuités majeures. De façon générale, l’urbanisation est contrastée, ce qui conforte l’idée selon laquelle ces espaces insulaires sont des objets hétérogènes, en dépit de leurs critères historiques et culturels homogènes (Note de l’auteur). Les mutations socioéconomiques observées ont des incidence...
Caraïbes

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.342
http://journals.openedition.org/etudescaribeennes/342

Éditeur(s) : HAL CCSD l'Harmattan
Résumé : International audience
La Martinique constitue l'un des trois départements français d'outre-mer de la zone Caraïbe-Amérique. Cette société est marquée dans sa période contemporaine, par le dynamisme de ses formes culturelles et par un essor notable des médias de masse. Les mutations rapides qui la travaillent en profondeur sous l'effet des activités d'information et des phénomènes de communication invitent au renouvellement de l'approche des cultures et des identités. Comment envisager la place de la médiatisation dans la discussion publique sur l'identité martiniquaise? On observe les représentations de l'identité dans l'espace public martiniquais à partir de la sphère médiatique. On s'intéresse ici aux modalités de circulation des messages relatifs aux cultures quant à leur rôle dans les processus de construction identitaire. Cette approche permet d'interroger les relations qui existent entre journalistes, personnel politique et opinion publique ; elle ouvre la réflexion sur le sens que les acteurs donnent au message médiatisé et, l'usage qu'ils en font dans un espace social donné. Un contexte sociopolitique pesant L'espace public martiniquais reste encore marqué actuellement, par le contexte sociopolitique local fortement politisé des années 1960. Les discours politiques de l'époque s'articulent d'abord sur la nature des relations à la Métropole et posent la question du changement de statut pour le département d'outre-mer. Ils se structurent aussi autour de préoccupations culturelles et visent la conservation du patrimoine.
Paroles d'outre-mer. Identités linguistiques, expressions littéraires, espaces médiatiques
Saint-Denis (La Réunion), France

hal-01152955
https://hal.archives-ouvertes.fr/hal-01152955
https://hal.archives-ouvertes.fr/hal-01152955/document
https://hal.archives-ouvertes.fr/hal-01152955/file/Identites_et_medias.pdf

Résumé : On August 18th, 1967, H.M. Arvid Pardo, Malta's delegate at the UN, referred to the General Assembly with the question entitled: "Examination of the question of the reservation exclusively for peaceful purposes of the sea-bed and the ocean floor, and the subsoil thereof, underlying the high seas beyond the limits of present national jurisdiction, and the use of their resources in the interests of mankind." (1) This proposal was not the result of pure abstract thinking, but was, on the contrary, the wording of the necessity to adapt marine legislation to the current development state of the international society. However, this evolutive perspective does not exist only economically and technically speaking: it develops in the political field, especially in the development of the nations claim over marine spaces and the genesis of the principle of freedom of high seas. As in any dialectical perspective, legislation interferes with these de facto situations and imposes a number of directions to the study of a legal regime responding to the needs of a changing international society.
Le 18 août 1967, SE Arvid PARDO, représentant de Malte à l'ONU, saisit l'Assemblée Générale de la question intitulée : "Examen de la question de l'affectation à des fins exclusivement pacifiques du lit des mers ainsi que de leur sous sol, au-delà des limites de la juridiction nationale actuelle et de l'exploitation de leurs ressources dans l'intérêt de l'humanité". (1) Cette proposition n'était pas l'émanation d'une réflexion purement abstraite, mais traduisait au contraire la nécessité d'adapter le droit de la mer à l'état de développement de la société internationale. Cependant, cette perspective évolutive n'existe pas seulement sur le plan économique et technologique : elle se développe au niveau politique, particulièrement dans l'extension des prétentions étatiques sur les espaces maritimes et la genèse du principe de liberté de la haute mer. Comme dans toute perspective dialectique, le droit interfère sur ces situations de fait et impose donc a priori certaines orientations à l'étude d'un régime juridique répondant aux besoins d'une société internationale en pleine mutation. [OCR NON CONTRÔLE]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1973/rapport-4803.PDF
http://archimer.ifremer.fr/doc/00000/4803/

Éditeur(s) : HAL CCSD
Résumé : International audience
Nous nous interrogeons sur le fonctionnement en Guadeloupe du brevet de technicien supérieur (BTS), diplôme professionnel français se préparant en deux ans après le baccalauréat, avec des programmes et examens nationaux. Les lauréats sont censés être directement employables, principalement dans leur environnement géographique immédiat. Nous postulons l’existence d’un paradoxe entre la définition nationale du diplôme et les contextes locaux d’enseignement et d’intégration professionnelle, que nous examinions au travers des pratiques déclarées d’enseignants. Une enquête par entretiens semi-directifs, menée auprès d’enseignants en sciences économiques et de gestion des BTS tourisme et assistant de manager, nous a permis de constater une prise en compte pragmatique, peu outillée sur le plan théorique, du contexte de la formation. Cependant, des limites à cette contextualisation sont observées. Par exemple, en BTS tourisme, un décalage est constaté, issu d’une mutation moins accomplie en Guadeloupe que dans l’Hexagone vers la tourismatique. En BTS assistant de manager, les enseignants sont confrontés aux spécificités du régime fiscal local qu’ils hésitent à enseigner de peur de pénaliser leurs étudiants lors des examens nationaux. Ces éléments confirment notre postulat sur l’existence de paradoxes, non résolus par l’institution, entre les visées d’insertion professionnelle locale des BTS et leurs prescrits non contextualisés.
4e colloque international en éducation, intitulé "Enjeux actuels et futurs de la formation et profession enseignante"
Montreal, Canada

hal-01535036
https://hal.univ-antilles.fr/hal-01535036

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: The meiotic developmental pathway in yeast enables both differentiation of vegetative cells into haploid spores that ensure long-term survival, and recombination of the parental DNA to create genetic diversity. Despite the importance of proper metabolic regulation for the supply of building blocks and energy, little is known about the reprogramming of central metabolic pathways in meiotically differentiating cells during passage through successive developmental stages. Results: Metabolic regulation during meiotic differentiation in budding yeast was analyzed by integrating information on genome-wide transcriptional activity, 26 enzymatic activities in the central metabolism, the dynamics of 67 metabolites, and a metabolic flux analysis at mid-stage meiosis. Analyses of mutants arresting sporulation at defined stages demonstrated that metabolic reprogramming is tightly controlled by the progression through the developmental pathway. The correlation between transcript levels and enzymatic activities in the central metabolism varies significantly in a developmental stage-dependent manner. The complete loss of phosphofructokinase activity at mid-stage meiosis enables a unique setup of the glycolytic pathway which facilitates carbon flux repartitioning into synthesis of spore wall precursors during the co-assimilation of glycogen and acetate. The need for correct homeostasis of purine nucleotides during the meiotic differentiation was demonstrated by the sporulation defect of the AMP deaminase mutant amd1, which exhibited hyper-accumulation of ATP accompanied by depletion of guanosine nucleotides. Conclusions: Our systems-level analysis shows that reprogramming of the central metabolism during the meiotic differentiation is controlled at different hierarchical levels to meet the metabolic and energetic needs at successive developmental stages.
ISSN: 1471-2180

inserm-01094132
http://www.hal.inserm.fr/inserm-01094132
http://www.hal.inserm.fr/inserm-01094132/document
http://www.hal.inserm.fr/inserm-01094132/file/s12915-014-0060-x.pdf
DOI : 10.1186/s12915-014-0060-x

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Dans un monde essentiellement mouvant et dynamique et dont les stratégies de développement sont tout aussi diverses, le monde rural ne reste pas à la traine. Les espaces ruraux qui sont par essence des lieux de survivances culturelles ont de la peine à construire des stratégies de développement par des replis identitaires dans un contexte de mondialisation et marqué particulièrement par une indigence notoire. Dans ce contexte, le tourisme qui demeure une situation de rente pour les ruraux est influencé par de profondes dynamiques exogènes. L’objectif de ce travail est donc d’évaluer le lien social entre les acteurs inscrits dans le développement du tourisme en milieu rural. La problématique est soutenue par les interrogations suivantes : comment résister aux conduites modernes des touristes en milieu rural dans un contexte de mondialisation ? Les modes de vie des touristes en milieu rural sont-ils autant compatibles avec ceux des locaux ? Comment les touristes influencent-ils les modes de vie des acteurs ruraux au point d’en provoquer des innovations socio-économiques ? Le cadre théorique est essentiellement axé sur la théorie du lien social axé sur la finalité de l’échange. La méthode est qualitative, car il est question dans cette étude de ressortir les interactions entre les acteurs en présence dans la problématique du tourisme. Les résultats montrent que : a) le tourisme en milieu rural est une source de rente pour les locaux, b) il y a une innovation dans la gestion des temps traditionnelles, c) les sources de revenus ne sont plus seulement issues de la production agricoles, mais aussi des services, d) les modes de vie des ruraux sont profondément modifiés par les dynamiques portées par les touristes.
In a changing world whose development strategies are diverse, the rural world is not left behind. Rural areas that essentially represent cultural resilience sites are challenged in building development strategies in a globalized world characterized by notorious poverty. In this context tourism, which remains an economic rent for rural, is influenced by exogenous conditions. This paper aims to analyze the social bond between the players enrolled in the development of rural tourism. The analytic process is supported by the following questions: How to resist the modern behaviour of rural tourists in a globalization context? Are the lifestyles of rural tourists compatible with locals? How do tourists influence the lifestyle of rural stakeholders to the point of causing socio-economic innovations? The theoretical framework focuses mainly on the theory of social ties focusing on the purpose of exchange. The method is qualitative, as this study highlights the interactions between the stakeholders. The results show that: a) Rural tourism is a source of income for locals; b) there is an innovation in managing traditional time; c) sources of income are not only from agricultural production but also from services; d) rural lifestyles are profoundly changed by the dynamic brought by tourists.
Cameroun
Kribi

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.8820
http://journals.openedition.org/etudescaribeennes/8820

Éditeur(s) : HAL CCSD
Résumé : International audience
The SOX8 and SOX9 transcription factors are involved in, among others, sex differentiation, male gonad development and adult maintenance of spermatogenesis. Sox8(-/-) mice lacking Sox9 in Sertoli cells fail to form testis cords and cannot establish spermatogenesis. Although genetic and histological data show an important role for these transcription factors in regulating spermatogenesis, it is not clear which genes depend upon them at a genome-wide level. To identify transcripts that respond to the absence of Sox8 in all cells and Sox9 in Sertoli cells we measured mRNA concentrations in testicular samples from mice at 0, 6 and 18 days post-partum. In total, 621 and 629 transcripts were found at decreased or increased levels, respectively, at different time points in the mutant as compared to the control samples. These mRNAs were categorized as preferentially expressed in Sertoli cells or germ cells using data obtained with male and female gonad samples and enriched testicular cell populations. Five candidate genes were validated at the protein level. Furthermore, we identified putative direct SOX8 and SOX9 target genes by integrating predicted SOX-binding sites present in potential regulatory regions upstream of the transcription start site. Finally, we used protein network data to gain insight into the effects on regulatory interactions that occur when Sox8 and Sox9 are absent in developing Sertoli cells. The integration of testicular samples with enriched Sertoli cells, germ cells and female gonads enabled us to broadly distinguish transcripts directly affected in Sertoli cells from others that respond to secondary events in testicular cell types. Thus, combined RNA profiling signals, motif predictions and network data identified putative SOX8/SOX9 target genes in Sertoli cells and yielded insight into regulatory interactions that depend upon these transcription factors. In addition, our results will facilitate the interpretation of genome-wide in vivo SOX8 and SOX9 DNA binding data.
Andrology

hal-00877013
https://hal.archives-ouvertes.fr/hal-00877013
DOI : 10.1111/j.2047-2927.2012.00049.x
PUBMED : 23315995

Éditeur(s) : HAL CCSD Society for Molecular Biology and Evolution
Résumé : International audience
Microsatellites (SSRs) are highly susceptible to expansions and contractions. When located in a coding sequence, the insertion or the deletion of a single unit for a mono-, di-, tetra-, or penta(nucleotide)-SSR creates a frameshift. As a consequence, one would expect to find only very few of these SSRs in coding sequences because of their strong deleterious potential. Unexpectedly, genomes contain many coding SSRs of all types. Here, we report on a study of their evolution in a phylogenetic context using the genomes of four primates: human, chimpanzee, orangutan, and macaque. In a set of 5,015 orthologous genes unambiguously aligned among the four species, we show that, except for tri- and hexa-SSRs, for which insertions and deletions are frequently observed, SSRs in coding regions evolve mainly by substitutions. We show that the rate of substitution in all types of coding SSRs is typically two times higher than in the rest of coding sequences. Additionally, we observe that although numerous coding SSRs are created and lost by substitutions in the lineages, their numbers remain constant. This last observation suggests that the coding SSRs have reached equilibrium. We hypothesize that this equilibrium involves a combination of mutation, drift, and selection. We thus estimated the fitness cost of mono-SSRs and show that it increases with the number of units. We finally show that the cost of coding mono-SSRs greatly varies from function to function, suggesting that the strength of the selection that acts against them can be correlated to gene functions.
ISSN: 1759-6653

hal-01544762
https://hal.archives-ouvertes.fr/hal-01544762
DOI : 10.1093/gbe/evt003

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
CYLD is a tumour suppressor gene mutated in familial cylindromatosis, a genetic disorder leading to the development of skin appendage tumours. It encodes a deubiquitinating enzyme that removes Lys63- or linear-linked ubiquitin chains. CYLD was shown to regulate cell proliferation, cell survival and inflammatory responses, through various signalling pathways. Here we show that CYLD localizes at centrosomes and basal bodies via interaction with the centrosomal protein CAP350 and demonstrate that CYLD must be both at the centrosome and catalytically active to promote ciliogenesis independently of NF-κB. In transgenic mice engineered to mimic the smallest truncation found in cylindromatosis patients, CYLD interaction with CAP350 is lost disrupting CYLD centrosome localization, which results in cilia formation defects due to impairment of basal body migration and docking. These results point to an undiscovered regulation of ciliogenesis by Lys63 ubiquitination and provide new perspectives regarding CYLD function that should be considered in the context of cylindromatosis.
ISSN: 2041-1723

hal-01063915
https://hal.archives-ouvertes.fr/hal-01063915
DOI : 10.1038/ncomms5585
PUBMED : 25134987