Éditeur(s) : HAL CCSD INVS
Résumé : National audience
Context – Cervical cancer is the second most frequent cancer of women in French Guiana. Studies have shown that populations living in remote villages in the interior of the territory have early sexual relations and that multiple sexual partnerships are frequent. Hence, the main objective of the study was to determine the prevalence of Human Papilloma Virus (HPV) in these areas.Methods – This multicentric cross sectional study was conducted in women aged 20 to 65 years, living in theremote villages along the Maroni and Oyapock rivers. Women were included on a voluntary basis after beinginformed of the study team schedule through radio address and key opinion leaders.The search for HPV and cytologic examinations were performed on each sample. HPV infection was defined bythe detection of viral DNA using the Greiner Bio One® kit. Cytologic analyses were performed using the 2001Bethesda classification.Results – Overall, 643 women were included in the study. Age-standardized HPV prevalence was 35%. Theprevalence curve by age group showed a U-shaped pattern: women over 50 years had the highest risk of being infected by HPV, followed by women aged 20-29 years. In addition, 26.1% of women with a normal smear test had HPV infection. The most frequent genotypes were HPV52, HPV58, and HPV16. On interpretable smears 1.16% of women had high grade superficial intra-epithelial (HSIL) lesions.Conclusions – The standardized prevalence of HPV in French Guiana is among the highest in the world. HSILcytological lesions were nearly 4 times more frequent than in the Île-de-France region. Given the importance of the incidence of cervical cancer in French Guiana, these results suggest it may be preferable to use HPV testing than cytology. Vaccination against HPV, preferably with the new nonavalent vaccine seems an appropriate prevention measure. However, this intervention will be a challenge to implement in this region where a significant proportion of the population is not covered by health insurance.
Contexte – Le cancer du col de l’utérus est le deuxième cancer féminin le plus fréquent en Guyane française.Des études ont montré que les populations résidant dans les communes isolées de l’intérieur du départementdébutent leur vie sexuelle de façon précoce et que le multipartenariat sexuel y est fréquent. Dans ce contexte, l’objectif principal de cette étude était de déterminer la prévalence de l’infection à papillomavirus humains (HPV) dans ces zones.Méthodes – Cette étude transversale multicentrique a été conduite auprès des femmes âgées de 20 à 65 ansrésidant dans les communes isolées des fleuves Maroni et Oyapock. Les femmes étaient incluses sur la basedu volontariat après avoir été informées du passage de l’équipe de l’étude via la radio locale et par les leadersd’opinion. La recherche d’infection à HPV et les analyses cytologiques ont été réalisées sur chaque prélèvement. L’infection à HPV était définie par la détection d’ADN viral par le kit de dépistage Greiner Bio One®. Les analyses cytologiques ont été décrites selon la classification de Bethesda 2001.Résultats – Au total, 643 femmes ont été incluses. La prévalence de l’infection à HPV, standardisée sur l’âge,était de 35%. La courbe de prévalence par classe d’âge présentait une forme en U : les femmes de plus de50 ans avaient le plus haut risque d’être infectées par HPV, suivies par les femmes âgées de 20 à 29 ans.Par ailleurs, 26,1% des femmes ayant un frottis normal étaient infectées par le HPV. Les génotypes les plusfréquents étaient HPV52, HPV58 et HPV16. Sur les frottis interprétables, 1,16% des femmes avaient des lésions de haut grade (type HSIL).Conclusions – Le taux standardisé de prévalence de l’infection par le HPV en Guyane est parmi les plus élevésau monde. Les lésions cytologiques de type HSIL étaient près de 4 fois plus fréquentes qu’en Île-de-France.Étant donné l’importance de l’incidence du cancer du col de l’utérus en Guyane, ces résultats montrent qu’il est peut être préférable d’utiliser le test HPV plutôt que la cytologie. La vaccination anti-HPV, de préférence avec le nouveau vaccin nonavalent, semble être une mesure de prévention appropriée. Pour autant, cela reste un challenge de mettre en oeuvre ces interventions dans cette région où une part importante de la population n’a pas d’assurance maladie.
ISSN: 1953-8030

Droits : info:eu-repo/semantics/OpenAccess
inserm-01407222
http://www.hal.inserm.fr/inserm-01407222
http://www.hal.inserm.fr/inserm-01407222/document
http://www.hal.inserm.fr/inserm-01407222/file/2016%2C%20Dufit%20et%20al.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Development of hepatocellular carcinoma (HCC) is a complex and progressive disease that involves cycles of liver cell death, inflammation, and tissue regeneration/remodelling. Chemokines and chemokine receptors play numerous and integral roles in the disease progression of HCC. Here we investigated the novel chemokine receptor CXCR7/RDC1 in HCC progression, its two known ligands CXCL12 and CXCL11, as well as the other CXCL12 receptor, CXCR4. Our results show that in a cohort of 408 human HCCs, CXCR7 and CXCL11 were significantly higher in tumours compared to normal liver controls (5- and 10-fold, respectively). Immunohistochemical (IHC) staining on human HCC sections confirmed that both CXCL11 and CXCR7 were much higher in cancer tissues. Furthermore, IHC staining revealed that CXCR7 protein was only expressed in endothelial cells whereas CXCL11 exhibited a much broader tissue expression. At the cellular level we observed that in vitro, human microvascular endothelial cells (HMEC-1) up-regulated CXCR7 under hypoxic and acidic pH conditions, which are well known characteristics of the HCC tumour micro-environment. As for its ligand, we observed that IFNγ robustly induced CXCL11 in hepatic stellate cells, hepatocytes, and HMEC-1s. In addition, in the mouse Diethylnitrosamine model of hepatocarcinogenesis we observed a very strong induction of CXCR7 and CXCL11 transcripts, confirming that CXCR7/CXCL11 up-regulation is conserved between human and mice liver cancer. Altogether, our results strongly support the hypothesis that the CXCL11/CXCR7 pathway is involved HCC progression.
ISSN: 0959-8049

hal-00682463
https://hal.archives-ouvertes.fr/hal-00682463
DOI : 10.1016/j.ejca.2011.06.044
PUBMED : 21778049

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : La notion du tourisme moteur de développement n’a pas survécu à la chute du communisme. Cette chute a rendu caduc, le rôle idéologique de ce secteur, symbole et acteur du libéralisme au temps de la guerre froide. De fait, le tourisme retrouve sa place « normale » parmi les vocations nationales des pays en développement : aide au développement et/ou lutte contre la pauvreté !Le Maroc, l’un des pionniers du libéralisme en Afrique, n’a pas échappé aux conséquences du suivisme économique propre aux pays d’Afrique, socialistes et libéraux confondus. Du tourisme moteur de développement (1964), il a récemment procédé au réajustement de sa stratégie en matière du développement touristique, en en faisant un secteur d’appoint pour l’économie nationale. « Le miracle économique du tourisme n’a donc pas eu lieu ! » (Hillali, 2007). Constat d’échec ou réajustement stratégique normal, la question est posée. À ce propos, deux initiatives méritent d’être citées :- Le tourisme représente un secteur d’activité qui procure emplois et revenus aux citadins démunis (artisanat, emplois peu qualifiés...) et aux populations rurales (gites, auberges, accompagnement...) dans le cadre de la nouvelle stratégie des Pays d’accueil touristiques (2003), du tourisme de niches et de l’aide aux jeunes entrepreneurs. - L’initiative nationale du développement humain (INDH), lancée en 2004, est censée épauler les nécessiteux dans cette politique sociale et économique de grande envergure.Or, en dépit des programmes bien planifiés, les résultats sont mitigés. Peut-être, est-il que la mise en pratique de ces nouvelles approches de développement local et ciblé (actions opérationnelles) et les méthodes d’évaluation qui en assurent le suivi (mesure des impacts) ne sont pas encore assez rôdées.
The concept of tourism as a driving force for development has not survived the fall of communism. The end of the Cold War rendered the ideological role of the tourism sector, as a main tool of liberalism, obsolete. Today, the most common setting for the tourism sector is in developing countries, as a tool for development and/or poverty reduction.Morocco, one of the pioneers of liberalism in Africa, has not escaped the consequences of the economic band wagonning among African states, socialists and liberals alike. A driving force for tourism development in the last century (1964), Morocco has recently readjusted its strategy to create an extra sector to contribute to the national economy. "The economic miracle of tourism has not taken place!"(Hillali, 2007). The question is posed: What is more appropriate, identifying failures or engaging in strategic readjustment? In this regard, two initiatives are worth mentioning: - Tourism is an industry that provides jobs and income for the urban (crafts, low-skilled jobs) and rural (cottages, inns, coaching) poor as part of the new strategy of the country’s tourist reception (2003), tourism niches and helping young entrepreneurs. - The National Initiative for Human Development (NIHD), launched in 2004, aims to support those in need in major social and economic policies.However, despite well-planned programs, the results have been mixed. Perhaps it is that the implementation of these new approaches to local development and targeted (operational activities) and evaluation methods that ensure monitoring (measurement of impacts) do not overlap enough.
Afrique
Maroc

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6563
http://journals.openedition.org/etudescaribeennes/6563

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We conducted a laser-equipped analog experiment aimed at quasi-continuously monitoring the growth of a dense population of normal faults in homogeneous conditions. To further understand the way geological faults progressively gain in slip and length as they accumulate more strain, we measured with great precision the incremental slip and length changes that the analog faults sustain as they grow. These measurements show that the analog faults share common features with the natural ones. In particular, during their growth, the faults develop and maintain cumulative slip profiles that are generally triangular and asymmetric. The growth takes place through two distinct phases: an initial, short period of rapid lateral lengthening, followed by a longer phase of slip accumulation with little or no lengthening. The incremental slip is found to be highly variable in both space (along the faults) and time, resulting in variable slip rates. In particular, ‘short- and long-term' slip rates are markedly different. We also find that slip measurements at local points on fault traces do not contain clear information on the slip increment repeat mode. Finally, while the fault growth process is highly heterogeneous when considered at the scale of a few slip events, it appears homogeneous and self-similar at longer time scales which integrate many slip increments. This is likely to be the result of a feedback between stress heterogeneities and slip development. The long-term scale homogeneity also implies that the long-term faulting process is primarily insensitive to the short-term heterogeneities that are rapidly smoothed or redistributed. We propose a new conceptual scenario of fault growth that integrates the above observations and we suggest that faults grow in a bimodal way as a result of a self-driven and self-sustaining process.
ISSN: 0012-821X

Droits : info:eu-repo/semantics/OpenAccess
insu-00351998
https://hal-insu.archives-ouvertes.fr/insu-00351998
https://hal-insu.archives-ouvertes.fr/insu-00351998/document
https://hal-insu.archives-ouvertes.fr/insu-00351998/file/SchlagenhaufEtAl2008_EPSL_author.pdf
DOI : 10.1016/j.epsl.2008.06.042

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Sur la terre ferme, la sécurité est devenue un thème récurrent de la vie politique, des imaginaires voire de la réalité. Dans le monde de la croisière au contraire, la communication vend un univers idyllique. Or la réalité de la vie à bord du navire n’est pas faite que de bonheur, et les armateurs, les voyagistes, les équipages et les autorités administratives anticipent des situations qui mettent en péril les passagers. La gestion des épidémies ne se limite pas à la grippe H1N1, moment exceptionnel qui cache la réalité fréquente des épidémies de gastro-entérite. Le quotidien sécuritaire est aussi constitué par les risques plus traditionnels : le feu et le naufrage. La croisière également s’accompagne de viols et violences sur des croisiéristes, voire de disparitions de passagers. Ces trois dangers de la croisière font l’objet de mesures de santé publique, de définition de normes de sécurité internationales depuis 1914, et de mesures législatives récentes, puisque le dernier texte a été promulgué par le président Obama en juillet 2010. L’Europe montre un retard important dans l’organisation de cette politique, gage de développement de l’industrie de la croisière.
On land, security has become a recurring theme in the political life of the imagination or reality. In the world of cruising the contrary, communication sells idyllic world. The reality of life on the ship is not made that happiness, and shipowners, operators, crews and administrative authorities anticipate situations that endanger passengers. Outbreak management is not limited to the H1N1 exceptional moment that hides the reality of frequent outbreaks of gastroenteritis. The daily safe is also constituted by the more traditional risks: fire and sinking. The cruise also accompanied by rape and violence on cruise or disappearances of passengers. These three dangers of the cruise are the subject of public health measures, definition of international safety standards since 1914, and recent legislation, since the final text was issued by President Obama in July 2010. Europe shows a significant delay in the organization of the political guarantee for the development of the cruise industry.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5220
http://journals.openedition.org/etudescaribeennes/5220

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : Mantle xenoliths in Pliocene alkali basalts of the eastern Betics (SE Iberia, Spain) are spinel ± plagioclase lherzolite, with minor harzburgite and wehrlite, displaying porphyroclastic or equigranular textures. Equigranular peridotites have olivine crystal preferred orientation (CPO) patterns similar to those of porphyroclastic xenoliths but slightly more dispersed. Olivine CPO shows [100]-fiber patterns characterized by strong alignment of [100]-axes subparallel to the stretching lineation and a girdle distribution of [010]-axes normal to it. This pattern is consistent with simple shear or transtensional deformation accommodated by dislocation creep. One xenolith provides evidence for synkinematic reactive percolation of subduction-related Si-rich melts/fluids that resulted in oriented crystallization of orthopyroxene. Despite a seemingly undeformed microstructure, the CPO in orthopyroxenite veins in composite xenoliths is identical to those of pyroxenes in the host peridotite, suggesting late-kinematic crystallization. Based on these observations, we propose that the annealing producing the equigranular microstructures was triggered by melt percolation in the shallow subcontinental lithospheric mantle coeval to the late Neogene formation of veins in composite xenoliths. Calculated seismic properties are characterized by fast propagation of P waves and polarization of fast S waves parallel to olivine [100]-axis (stretching lineation). These data are compatible with present-day seismic anisotropy observations in SE Iberia if the foliations in the lithospheric mantle are steeply dipping and lineations are subhorizontal with ENE strike, implying dominantly horizontal mantle flow in the ENE-WSW direction within vertical planes, that is, subparallel to the paleo-Iberian margin. The measured anisotropy could thus reflect a lithospheric fabric due to strike-slip deformation in the late Miocene in the context of WSW tearing of the subducted south Iberian margin lithosphere.
ISSN: 0278-7407

Droits : info:eu-repo/semantics/OpenAccess
hal-01685809
https://hal.archives-ouvertes.fr/hal-01685809
https://hal.archives-ouvertes.fr/hal-01685809/document
https://hal.archives-ouvertes.fr/hal-01685809/file/Hidas_et_al-2016-Tectonics.pdf
DOI : 10.1002/2016TC004165

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We present GPS observations in Morocco and adjacent areas of Spain from 15 continuous (CGPS) and 31 survey-mode (SGPS) sites extending from the stable part of the Nubian plate to central Spain. We determine a robust velocity field for the W Mediterranean that we use to constrain models for the Iberia-Nubia plate boundary. South of the High Atlas Mountain system, GPS motions are consistent with Nubia plate motions from prior geodetic studies. We constrain shortening in the Atlas system to <1.5 mm/yr. 95% confidence level. North of the Atlas Mountains, the GPS velocities indicate Nubia motion with respect to Eurasia, but also a component of motion normal to the direction of Nubia-Eurasia motion, consisting of southward translation of the Rif Mountains in N Morocco at rates exceeding 5 mm/yr. This southward motion appears to be directly related to Miocene opening of the Alboran Sea. The Betic Mountain system north of the Alboran Sea is characterized by WNW motion with respect to Eurasia at 1-2 mm/yr. paralleling Nubia-Eurasia relative motion. In addition, sites located in the Betics north of the southerly moving Rif Mountains also indicate a component of southerly motion with respect to Eurasia. We interpret this as indicating that deformation associated with Nubia-Eurasia plate motion extends into the southern Betics, but also that the Betic system may be affected by the same processes that are causing southward motion of the Rif Mountains south of the Alboran Sea. Kinematic modeling indicates that plate boundary geometries that include a boundary through the Straits of Gibraltar are most compatible with the component of motion in the direction of relative plate motion, but that two additional blocks (Alboran-Rif block, Betic Mountain block), independent of both Nubia and Eurasia are needed to account for the motions of the Rif and Betic Mountains normal to the direction of relative plate motion. We speculate that the southward motions of the Alboran-Rif and Betic blocks may be related to mantle flow, possibly induced by southward rollback of the subducted Nubian plate beneath the Alboran Sea and Rif Mountains.
ISSN: 0264-3707

hal-00475538
https://hal.archives-ouvertes.fr/hal-00475538
DOI : 10.1016/j.jog.2009.10.007

Éditeur(s) : HAL CCSD Centre National de la Recherche Scientifique
Résumé : International audience
In the fragmented colonial society of the West Indies during the slavery period, building up a stable family required to get over many obstacles. The status differences, the lack of fluidity in the marriage market and the deep redefinition of the gender relations are interrelated phenomenon which question both the marriage as being the unique pattern of family organization and the structuration of family ties. If the official conjugal model remains the norm for white Creole women, it is not true neither for their husband nor for free coloured people and slaves. Therefore, the expansion of adultery and people living as husband and wife, which were denounced particularly by the religious authorities, lead to a high rate and continuous growth of illegitimate births. As a consequence, family ties gradually develop with no institutional control, hence no traces of them in the different sources but it also gives them an obvious flexibility, especially for the slaves.
Dans les sociétés coloniales fragmentées des Antilles de la période esclavagiste, les obstacles à la constitution de familles stables sont nombreux. Les différences de statut, le manque de fluidité du marché matrimonial, et la redéfinition profonde des rapports de genre sont autant de phénomènes articulés qui remettent en cause le mariage comme élément unique d'organisation de la famille et de structuration des relations de parenté. Si le modèle conjugal reconnu par les autorités reste la norme obligée pour les femmes blanches créoles, il n'en est pas de même pour leurs maris et pour les populations de couleur libre ou esclave. Aussi, l'essor du concubinage et des relations adultérines, dénoncé en particulier par les autorités religieuses, se concrétise par une illégitimité des naissances élevée et en croissance continue. Les liens familiaux se construisent ainsi en partie en dehors du contrôle institutionnel, ce qui leur confère une invisibilité dans les sources, mais aussi une souplesse certaine, particulièrement chez les esclaves.
Annales de Demographie Historique

hal-00753581
https://hal-unilim.archives-ouvertes.fr/hal-00753581

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Mechanical forces influence the growth and shape of virtually all tissues and organs. Recent studies show that increased cell contractibility, growth and differentiation might be normalized by modulating cell tensions. Particularly, the role of these tensions applied by the extracellular matrix during liver fibrosis could influence the hepatocarcinogenesis process. The objective of this study is to determine if 3D stiffness could influence growth and phenotype of normal and transformed hepatocytes and to integrate extracellular matrix (ECM) stiffness to tensional homeostasis. We have developed an appropriate 3D culture model: hepatic cells within three-dimensional collagen matrices with varying rigidity. Our results demonstrate that the rigidity influenced the cell phenotype and induced spheroid clusters development whereas in soft matrices, Huh7 transformed cells were less proliferative, well-spread and flattened. We confirmed that ERK1 played a predominant role over ERK2 in cisplatin-induced death, whereas ERK2 mainly controlled proliferation. As compared to 2D culture, 3D cultures are associated with epithelial markers expression. Interestingly, proliferation of normal hepatocytes was also induced in rigid gels. Furthermore, biotransformation activities are increased in 3D gels, where CYP1A2 enzyme can be highly induced/activated in primary culture of human hepatocytes embedded in the matrix. In conclusion, we demonstrated that increasing 3D rigidity could promote proliferation and spheroid developments of liver cells demonstrating that 3D collagen gels are an attractive tool for studying rigidity-dependent homeostasis of the liver cells embedded in the matrix and should be privileged for both chronic toxicological and pharmacological drug screening.
ISSN: 0730-2312

hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/file/Increasing%203D%20Matrix%20Rigidity_accepted.pdf
DOI : 10.1002/jcb.25356

Résumé : The present report synthesizes the results of the works led in collaboration between the IFREMER, the Hydrographic and Oceanographic service of the French Navy (Oceanographic Mission of the Pacific) and the Departments of the Sea and Fish farming and of the Management and Town planning of the Territory of the French Polynesia. After a reminder of the problems regarding morphological and hydrographic mapping of low islands (atolls) of the Pacific in particular in French Polynesia, a paragraph draws up the assets of the aerial and spatial images as well as the computer-assisted digital mapping. A chapter of the report is then dedicated to the data, to the digital processing methodologies and to the display of the results. The following one develops the applications in particular relative to 4 types of by-products of the images and the (processing : i) the spacemap said "Pacific standard ", ii) said spacemap "maritime leases", iii) the geographical database of the information system SIGMA POE RAVA dedicated to the management of the pearl oyster culture and iiii) the production of bathymetric meshed models. A conclusion approaches the perspectives and the potential developments.
Le présent rapport synthétise les résultats des travaux menés en collaboration entre l'IFREMER, le Service Hydrographique et Océanographique de la Marine (Mission Océanographique du Pacifique) et les Services de la Mer et de l'Aquaculture et de l'Aménagement et de l'Urbanisme du Territoire de la Polynésie française. Après un rappel de la problématique posée en matière de cartographie morphologique et hydrographique des iles basses (atolls) du Pacifique et notamment de Polynésie française, un paragraphe dresse les atouts de l’image aérienne et spatiale ainsi que de la cartographie numérique assistée par ordinateur. Un chapitre du rapport est consacré aux données, aux méthodologies de traitement et à la présentation des résultats. Le suivant développe les applications notamment relatives à 4 types de produits dérivés des images et des traitements : i) la spatiocarte dite «à norme Pacifique», ii) les spatiocartes dites «concessions maritimes», iii) la base géographique du système d’information SIGMA Poe Rava dédié à la gestion de la perliculture et iiii) la production de modèles bathymétriques maillés».Une conclusion aborde les perspectives et développements potentiels

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00195/30644/29099.pdf
http://archimer.ifremer.fr/doc/00195/30644/

Éditeur(s) : HAL CCSD Elsevier
Résumé : The dense hydrous magnesium silicate called the D phase is the most likely candidate to recycle hydrogen into the lower mantle in subduction zones. As seismology represents the preferred method to detect this mineral in subduction zones, the single crystal elastic tensor has been calculated using first principle methods. A triple cell (View the MathML source) was used to account for an ordered ideal composition of the D phase to lower mantle pressures. The elastic tensor of the mineral at ambient conditions is predicted to be C11 = 387.7, C33 = 287.7, C44 = 100.4, C12 = 108.0, C13 = 51.1, C14 = − 14.6 in GPa with bulk modulus at zero pressure Ko = 163 GPa and a statistical error of about 4.0 GPa for all constants. The pressure derivatives of the elastic constants have been determined to pressure of 80 GPa. Our tensor correctly predicts the published experimental measurements of linear compressibility along the a- and c-axes, the logarithmic pressure derivative of the c/a axial ratio and bulk modulus at zero pressure. It contradicts the experimentally measured elastic tensor reported by (Liu, L.-G., Okamoto, K., Yang, Y.-J., Chen, C.-C., Lin, C.-C., 2004. Elasticity of single-crystal phase D (a dense hydrous magnesium silicate) by Brillouin spectroscopy. Solid State Commun. 132, 517–520), which fails to reproduce the experimental compressibility data. Using the elastic constants and density predicted to 85 GPa, the seismic anisotropy of P- and S-waves has been calculated as a function of pressure. The single crystal has high P-wave velocities and shear wave splitting in the basal plane below 20 GPa. At high pressures, the maximum P-wave velocities have threefold symmetry normal to a-axes in the basal plane, whereas the maximum shear wave splitting is parallel to a-axes with twofold symmetry. The D phase has high Vp and Vs anisotropy at ambient pressure, 17.6% for Vp and 19.9% for Vs. The Vp anisotropy decreases with increasing pressure to about 30 GPa, while it remains constant at 8% from 30 to 85 GPa. The Vs anisotropy decreases to 17% at 20 GPa and then increases to 22% at 85 GPa. The isotropic velocity ratios of S to P (Rs/p), bulk sound to S (Rphi/s) and density to S (Rρ/s) are calculated as a function of pressure. The Rs/p shows very little variation with pressure with low value of 0.6, whereas both Rphi/s and Rρ/s increase with pressure to peak values of 2.8 and 2.0 respectively at about 60 GPa. The presence of the D phase in subducted horizontal ‘stagnant' plates in the circum Pacific could contribute to a number of seismic observations, such as the observed velocity heterogeneity at depths between 500 and 1000 km and the fast S-waves travelling horizontally with horizontal polarization in this depth range. Further the lower density of the D phase compared with anhydrous minerals could influence buoyancy of the hydrated ‘stagnant' slabs. The transformation of the D phase to perovskite plus ferripericlase during thermal annealing may be responsible for horizontal slab seismogenesis.
ISSN: 0012-821X

hal-00406379
https://hal.archives-ouvertes.fr/hal-00406379
DOI : 10.1016/j.epsl.2007.04.053

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Transcription factor GATA4 is required for the development and function of the mammalian gonads. We first reported that the GATA4 gene in both human and rodents is expressed as two major alternative transcripts that differ solely in their first untranslated exon (exon 1a vs. exon 1b). We had also showed by quantitative PCR that in mouse tissues, both Gata4 exon 1a- and 1b-containing transcripts are present in all sites that are normally positive for GATA4 protein. In adult tissues, exon 1a-containing transcripts generally predominate. A notable exception, however, is the testis where the Gata4 exon 1a and 1b transcripts exhibit a similar level of expression. We now confirm by in situ hybridization analysis that each transcript is also strongly expressed during gonad differentiation in both sexes in the rat. To gain further insights into how Gata4 gene expression is controlled, we characterized the mouse Gata4 promoter sequence located upstream of exon 1b. In vitro studies revealed that the Gata4 1b promoter is less active than the 1a promoter in several gonadal cell lines tested. Whereas we have previously shown that endogenous Gata4 transcription driven by the 1a promoter is dependent on a proximally located Ebox motif, we now show using complementary in vitro and in vivo approaches that Gata4 promoter 1b-directed expression is regulated by GATA4 itself. Thus, Gata4 transcription in the gonads and other tissues is ensured by distinct promoters that are regulated differentially and independently.
ISSN: 0006-3363

hal-01010626
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01010626
DOI : 10.1095/biolreprod.113.113290
PUBMED : 24352556

Éditeur(s) : Marine Biological Laboratory
Résumé : Hydrothermal vent tubeworms, Riftia pachyptila Jones, were maintained alive and studied on board ship using flow-through pressure aquaria. Simultaneous measurements of O2, SIGMA-CO2, SIGMA-H2S fluxes showed that the intact symbioses reach maximum rates of uptake of SIGMA-CO2 (>2 mu-mole g-1 h-1) at about 90 mu-M SIGMA-H2S. Measurements were made of hemolymph and coelomic fluid SIGMA-CO2, SIGMA-H2S, thiosulfate, pH, and hemoglobin concentrations in worms kept under various conditions of O2 and SIGMA-H2S. Normal hemolymph pH appears to be about 7.5 and is not affected by SIGMA-H2S and SIGMA-CO2 concentrations within the ranges observed. We conclude that Riftia is specialized to provide sulfide to its symbionts with minimal interaction of sulfide with the animal metabolism. The uptake of sulfide is apparently by diffusion into the hemolymph, facilitated by the sulfide-binding properties of the hemoglobins. Both SIGMA-CO2 and P(CO2) are elevated in the hemolymph above their levels in the medium, although they are reduced under autotrophic conditions. Thus inorganic carbon is apparently concentrated from the medium into the hemolymph by an unknown mechanism.
Biological Bulletin (0006-3185) (Marine Biological Laboratory), 1991-02 , Vol. 180 , N. 1 , P. 135-153

Droits : 1991 Marine Biological Laboratory
http://archimer.ifremer.fr/doc/00178/28970/27392.pdf
DOI:10.2307/1542437
http://archimer.ifremer.fr/doc/00178/28970/

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option B
Résumé : International audience
To recover verticality after disturbance, angiosperm trees produce " tension wood " allowing them to bend actively. The driving force of the tension has been shown to take place in the G-layer, a specific unlignified layer of the cell wall observed in most temperate species. However, in tropical rain forests, the G-layer is often absent and the mechanism generating the forces to reorient trees remains unclear. A study was carried out on tilted seedlings, saplings and adult Simarouba amara trees – a species known to not produce a G-layer. Microscopic observations were done on sections of normal and tension wood after staining or observed under UV light to assess the presence/absence of lignin. We showed that S. amara produces a cell-wall layer with all of the characteristics typical of G-layers, but that this G-layer can be observed only as a temporary stage of the cell-wall development because it is masked by a late lignification. Being thin and lignified, tension wood fibres cannot be distinguished from normal wood fibres in the mature wood of adult trees. These observations indicate that the mechanism generating the high tensile stress in tension wood is likely to be the same as that in species with a typical G-layer and also in species where the G-layer cannot be observed in mature cells.
ISSN: 0829-318X

hal-01229280
https://hal.archives-ouvertes.fr/hal-01229280
https://hal.archives-ouvertes.fr/hal-01229280/document
https://hal.archives-ouvertes.fr/hal-01229280/file/Roussel-Clair-2015-TreePhysiol-LateLignifGLayerSimarouba_preprintHAL.pdf
DOI : 10.1093/treephys/tpv082

Éditeur(s) : HAL CCSD
Résumé : This study is designed to improve the understanding of structural, mechanical and petrophysical characteristics of deformation bands in porous sandstone such as their potential influence on fluid storage or migration in reservoir setting. Structural and petrophysical analysis of band networks from Provence show that permeability of band is mainly controlled by the degree of cataclasis and diagenetic processes at depth. Map-scale analysis of band networks underlines the major influence of tectonic regime on band distribution, organization and permeability. Band associated to normal-fault regime (cataclasis and low-permeability) are located around map-sclae-fault whereas band associated to thurst-fault regime (moderate cataclasis and permeability) are pervasively distributed in all the area. Reverse-sense band are cataclastic and low-permeabiilty around the Roquemaure thrust, which show the potential role of large-scale fault on structural and petrophysical characteristics of bands. This analysis also shows the influence of fault propagation on formation of low permeability catacastic bands. This analysis suggests also granulometry as factor controlling organization of band networks.A mechanical analysis of deformation bands is realized based on results from Provence. Yield envelopes of these materials are calculated from analytical solution. Triaxial tests done on sandstone (L'Etang quarry, Orange) confirm the shape of yield envelope theoretically calculated. Triaxial tests done on the poorly-lithified sands (Boncavaï quarry, Uchaux) show grain size, sorting and packing influencing yield envelope and cataclastic band localization at shallow burial depth. Stress paths are calculated for burial and tectonic event (extension and contraction) using the relationship between main stress and secondary stresses (K0) calibrated from well data. These different results are integrated into a model which allows to estimate type and structural characteristics of bands susceptible to form for a known geological setting.A structural, mechanical and petrophysical model is established form these results. This model is calibrated by permeability data from deformation bands of Provence and results from the mechanical model of strain localization. This model is also confronted to a synthesis of permeability data from literature, which confirms the influence of the different controlling parameters integrated to the model. This model is applied for two sites of uranium exploration/exploitation in porous sandstone reservoirs and validated by field observations.
Cette étude est destinée à améliorer la compréhension des caractéristiques structurales, pétrophysiques et mécaniques des bandes de déformation dans les grès poreux ainsi que de leur rôle potentiel sur la migration ou le piégeage des fluides en milieu réservoir. L'analyse structurale et pétrophysique des réseaux de bandes de Provence montre que leur perméabilité découle principalement de l'intensité de la cataclase dans leur microstructure et des processus diagénétiques qui peuvent s'y localiser avec la profondeur. L'analyse régionale de ces réseaux de bandes souligne l'influence majeure du régime tectonique sur leur distribution, leur organisation et leur perméabilité. Les bandes associées au régime normal (cataclactiques et peu perméables) sont localisées autour des failles cartographiques, tandis que les bandes associées au régime inverse (modérement cataclastiques et perméables) sont distribuées dans toute la région. Les bandes inverses sont fortement cataclastiques et imperméables uniquement autour du chevauchement de Roquemaure ce qui montre le rôle potentiel de la présence de grande faille sur les caractéristiques structurales et pétrophysiques de ces bandes. D'autre part, cette analyse suggère que la granulométrie de l'encaissant peut influencer l'initiation et l'organisation des réseaux. L'analyse mécanique des bandes de déformation a ensuite été réalisée à partir des résultats obtenus en Provence. L'enveloppe de plasticité de ces matériaux est calculée à l'aide d'une solution analytique. Les essais triaxiaux menés sur le grès de la carrière de l'Etang (orange) confirment la forme de ces enveloppes estimées théoriquement. Les essais réalisés sur les sables de Boncavaï (Uchaux) montrent que la taille des grains, le tri et de la compaction dans ces matériaux peu lithifiés influent sur leurs enveloppes de plasticité et la localisation des bandes cataclastiques à faibles profondeurs. Les trajets de contraintes sont estimés pour les phases d'enfouissement et de chargement tectonique (extension et compression) à partir de la relation entre contrainte principale et contraintes secondaires (K0), calibrée à partir de données de forage. Ces résultats sont intégrés à un modèle permettant d'estimer la nature et les caractéristiques structurales des bandes susceptibles de se former dans un contexte géologique donné. Un modèle structural, mécanique et pétrophysique a été établir à partir de ces différents résultats. Ce modèle est calibré à partir des données de perméabilité mesurées sur les bandes de Provence et des résultats du modèle mécanique de localisation cité précédemment. Ce modèle a ensuite été confronté à une synthèse des données de perméabilité disponibles dans la littérature et confirme l'influence des différents paramètres de contrôle pris en compte dans le modèle. Ce modèle a enfin pu être appliqué sur deux sites d'explorations/exploitations d'uranium en réservoir gréseux poreux et validé par les résultats obtenus, en concordance avec les observations de terrain.
https://tel.archives-ouvertes.fr/tel-01001389

NNT : 2013MON20067
tel-01001389
https://tel.archives-ouvertes.fr/tel-01001389
https://tel.archives-ouvertes.fr/tel-01001389/document
https://tel.archives-ouvertes.fr/tel-01001389/file/36072_BALLAS_2013_archivage-1_cor.pdf

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Homologous recombination is the key process that generates genetic diversity and drives evolution. SPO11 protein triggers recombination by introducing DNA double stranded breaks at discreet areas of the genome called recombination hotspots. The hotspot locations are largely determined by the DNA binding specificity of the PRDM9 protein in human, mice and most other mammals. In budding yeast Saccharomyces cerevisae, which lacks a Prdm9 gene, meiotic breaks are formed opportunistically in the regions of accessible chromatin, primarily at gene promoters. The genome-wide distribution of hotspots in this organism can be altered by tethering Spo11 protein to Gal4 recognition sequences in the strain expressing Spo11 attached to the DNA binding domain of the Gal4 transcription factor. To establish whether similar re-targeting of meiotic breaks can be achieved in PRDM9-containing organisms we have generated a Gal4BD-Spo11 mouse that expresses SPO11 protein joined to the DNA binding domain of yeast Gal4. RESULTS: We have mapped the genome-wide distribution of the recombination initiation sites in the Gal4BD-Spo11 mice. More than two hundred of the hotspots in these mice were novel and were likely defined by Gal4BD, as the Gal4 consensus motif was clustered around the centers in these hotspots. Surprisingly, meiotic DNA breaks in the Gal4BD-Spo11 mice were significantly depleted near the ends of chromosomes. The effect is particularly striking at the pseudoautosomal region of the X and Y chromosomes -- normally the hottest region in the genome. CONCLUSIONS: Our data suggest that specific, yet-unidentified factors influence the initiation of meiotic recombination at subtelomeric chromosomal regions.
ISSN: 1471-2164

inserm-00849779
http://www.hal.inserm.fr/inserm-00849779
http://www.hal.inserm.fr/inserm-00849779/document
http://www.hal.inserm.fr/inserm-00849779/file/1471-2164-14-493.pdf
DOI : 10.1186/1471-2164-14-493

Éditeur(s) : HAL CCSD
Résumé : International audience
In the external Western Alps, the collision started with the shortening of the proximal European (Dauphinois) margin. One peculiar feature is the crustal thickening that finally led to the exhumation of the basement External Crystalline Massifs (ECM) during the Neogene. In these massifs, various age of shortening, structural style and sequence of deformation have been proposed leading to different kinematics along the Alpine arc that in turn do not imply the same crustal/lithospheric strength. In this contribution, we present new structural and microstructural data along with Ar/Ar ages from field studies in the Oisans-Grandes Rousses massifs, between Grenoble and Briançon). From balanced cross-sections, we show that about half of the external zone crustal shortening is accommodated within the ECM with similar Ar/Ar ages in all massifs. Furthermore, the shear zones present very large thicknesses (several hundred meters) and do not reactivated either the inherited Liassic normal faults or the Variscan foliation. Finally, the main shortening structures are localized beneath inherited Liassic syn-rift basins. These results suggest that the crust was weak during its shortening. Such behavior is most likely due to the combination of several factors: the crust burial (below the overthrusting internal units) down to about 10km, the presence of weak inherited syn-rift basins, and the strain softening in shear zones that evolve in mica-rich phyllonites. These results are consistent with the studies in the Aar massif and should most likely apply to the Mont Blanc massif, whose relationships with its cover are still debated.
EGU General Assembly
Vienne, Austria

insu-00839426
https://hal-insu.archives-ouvertes.fr/insu-00839426
BIBCODE : 2013EGUGA..15.8837B

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
UNLABELLED: ABSTRACT: BACKGROUND: Genomics, transcriptomics and proteomics of glioblastoma multiforme (GBM) have recently emerged as possible tools to discover therapeutic targets and biomarkers for new therapies including immunotherapy. It is well known that macroscopically complete surgical excision, radiotherapy and chemotherapy have therapeutic limitations to improve survival in these patients. In this study, we used a differential proteomic-based technique (2D-Difference Gel Electrophoresis) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry to identify proteins that may serve as brain tumor antigens in new therapeutic assays. Five samples of patients presenting a GBM and five samples of microscopically normal brain tissues derived from brain epileptic surgery specimen were labeled and run in 2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis) with an internal pool sample on each gel. Five gels were matched and compared with DIA (Difference In-gel Analysis) software. Differential spots were picked, in-gel digested and peptide mass fingerprints were obtained. RESULTS: From 51 protein-spots significantly up-regulated in GBM samples, mass spectrometry (MS) identified twenty-two proteins. The differential expression of a selected protein set was first validated by western-blotting, then tested on large cohorts of GBM specimens and non-tumor tissues, using immunohistochemistry and real-time RT-PCR. CONCLUSIONS: Our results confirmed the importance of previously described proteins in glioma pathology and their potential usefulness as biological markers but also revealed some new interesting targets for future therapies.
ISSN: 1477-5956

inserm-00585020
http://www.hal.inserm.fr/inserm-00585020
http://www.hal.inserm.fr/inserm-00585020/document
http://www.hal.inserm.fr/inserm-00585020/file/1477-5956-9-16.pdf
DOI : 10.1186/1477-5956-9-16

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
The objective of this study was to realize a three-dimensional (3-D) geological model of the deep basin structure of the Middle Durance region (of folds and faults) by integration of geological and geophysical data, and to evaluate its fault geometry and tectonic history. All of the available geophysical and geological data were compiled in three dimensions using the gOcad geomodeler. The geological and geophysical data were used to build a 3-D geological model of the Middle Durance region. The data on the 3-D geometry of fault surfaces and stratigraphic horizons and the thickness maps of the main stratigraphic units are supported by the 3-D geological model. We show that the Middle Durance Fault cannot be interpreted as a single fault plane that affected the entire Meso-Cenozoic sedimentary layers and the Paleozoic basement but as a listric segmented faulting system in sedimentary layers, rooted in Triassic evaporites and a normal block faulting system in the basement. This decoupling level in the Triassic layers reveals thin-skin deformation, formed by strong mechanical decoupling between the Mesozoic sedimentary cover and the Paleozoic basement. This study also confirms that the Provence geological structure has resulted mainly from Pyrenean deformation, which was partly reactivated by Alpine deformation. We demonstrate that the Middle Durance Fault Zone is a transfer fault that accommodates deformation of the sedimentary filling of the South-East Basin through modified fold geometry over a zone of 7 km to 8 km around the main segment of the fault zone.
ISSN: 0278-7407

hal-01172221
https://hal.archives-ouvertes.fr/hal-01172221
DOI : 10.1002/2014TC003749

Éditeur(s) : Gauthier-Villars
Résumé : In the sedimentary areas surrounding the Florida Keys, Soritidae and Peneroplidae are very well represented. These benthic foraminifera are generally attached to seagrasses or algae and more rarely to carbonate deposits. The colouring of their protoplasm results from the nature of endosymbiotic algae. The principal species represented are: Androsina lucasi (never recorded before in Florida) which lives only in the margino-littoral biotopes where the salinity is highly variable; Archaias angulatus and Amphisorus hemprichii which live in the biotopes of the littoral platform but also in those of the interior margin of the bay where the variations of salinity are weak; Cyclorbiculina compressa and Peneroplis proteus which live for the main part in the littoral biotopes of the oceanic platform where the salinity is normal and quite constant; five other species of secondary importance take the number of taxa observed alive in the Keys area up to ten. Moreover, if the comparison of the biocenoses and thanatocenoses emphasizes their comparative similitude, knowledge of the biocenoses permits a better understanding of their adaptation to present-day environments.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1991 , Vol. 14 , N. 5 , P. 515-524

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00103/21379/18979.pdf
http://archimer.ifremer.fr/doc/00103/21379/