Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Benzo[a]pyrene (B[a]P), the prototype molecule of polycyclic aromatic hydrocarbons, exhibits genotoxic and carcinogenic effects, which has led the International Agency for Research on Cancer to recognize it as a human carcinogen. Besides the well-known apoptotic signals triggered by B[a]P, survival signals have also been suggested to occur, both signals likely involved in cancer promotion. Our previous work showed that B[a]P induced an hyperpolarization of mitochondrial membrane potential (ΔΨm) in rat hepatic epithelial F258 cells. Elevated ΔΨm plays a role in tumor development and progression, and nitric oxide (NO) has been suggested to be responsible for increases in ΔΨm. The present study therefore aimed at evaluating the impact of B[a]P on NO level in F258 cells, and at testing the putative role for NO as a survival signal, notably in link with ΔΨm. Our data demonstrated that B[a]P exposure resulted in an NO production which was dependent upon the activation of the inducible NO synthase. This enzyme activation involved AhR and possibly p53 activation. Preventing NO production not only increased B[a]P-induced cell death but also blocked mitochondrial hyperpolarization. This therefore points to a role for NO as a survival signal upon B[a]P exposure, possibly targeting ΔΨm
ISSN: 0887-2333

hal-01163757
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757/file/Benzo%5Ba%5Dpyrene-induced%20nitric.pdf
DOI : 10.1016/j.tiv.2015.06.010

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : Vertical displacements on the SW-NE T (e) over capt fault (Eastern Pyrenees Axial Zone, France), which separates the Variscan Canigou-Caranca and Mont-Louis massifs, were constrained using a thermochronologic multi-method approach. Ar-40/Ar-39 data from the granitic Mont-Louis massif record its Variscan cooling history and reveal no ages younger than Early Cretaceous, while the Canigou-Caranca gneiss massif records systematically younger Ar-40/Ar-39 ages. These younger Ar-40/Ar-39 ages in the Canigou-Caranca gneiss massif are the result of partial to total rejuvenation of argon isotopic systems related to a thermal flow coeval with the Cretaceous HT-BP metamorphism in the North Pyrenean Zone. Only the deepest rocks from the Canigou-Caranca suffered this extensive Mid-Cretaceous thermal overprint probably due to differential burial around 4 km at that time. The post Mid-Cretaceous vertical displacements along the T (e) over capt fault are recorded by "low" temperature thermochronology using K-feldspar Ar-40/Ar-39, zircon and apatite fission track and (U-Th)/He datings. The Mont-Louis granite samples experienced a long period of protracted cooling reflecting a lack of thermo-tectonic activity in this area from Late Palaeozoic to Early Cenozoic, followed by cooling from 55-60 Ma to Late Eocene at a mean rate of 15-20 degrees C/Ma in the final stage. This cooling stage corresponds to T (e) over capt fault reactivation with a reversed component, promoting exhumation of the Mont-Louis roof zone contemporaneously with the south-vergent Pyrenean thrusting. In the Canigou-Caranca massif, the main cooling event occurred from 32 to 18 Ma at a maximum rate of 30 degrees C/Ma during Early Oligocene followed by a more moderate rate of 3 degrees C/Ma from Late Oligocene to Early Burdigalian, coeval with the normal reactivation of the T (e) over capt fault in brittle conditions that accommodated the final exhumation of the massif during the opening of the Gulf of Lion.
ISSN: 1437-3254

hal-00411442
https://hal.archives-ouvertes.fr/hal-00411442
DOI : 10.1007/s00531-007-0179-x

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Tropical cyclone (TC) waves can severely damage coral reefs. Models that predict where to find such damage (the 'damage zone') enable reef managers to: 1) target management responses after major TCs in near-real time to promote recovery at severely damaged sites; and 2) identify spatial patterns in historic TC exposure to explain habitat condition trajectories. For damage models to meet these needs, they must be valid for TCs of varying intensity, circulation size and duration. Here, we map damage zones for 46 TCs that crossed Australia's Great Barrier Reef from 1985–2015 using three models – including one we develop which extends the capability of the others. We ground truth model performance with field data of wave damage from seven TCs of varying characteristics. The model we develop (4MW) out-performed the other models at capturing all incidences of known damage. The next best performing model (AHF) both under-predicted and over-predicted damage for TCs of various types. 4MW and AHF produce strikingly different spatial and temporal patterns of damage potential when used to reconstruct past TCs from 1985–2015. The 4MW model greatly enhances both of the main capabilities TC damage models provide to managers, and is useful wherever TCs and coral reefs co-occur.
ISSN: 2045-2322

Droits : http://creativecommons.org/licenses/by/
hal-01330362
https://hal-univ-perp.archives-ouvertes.fr/hal-01330362
https://hal-univ-perp.archives-ouvertes.fr/hal-01330362/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01330362/file/srep26009.pdf
DOI : 10.1038/srep26009

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : This document shows several of the main findings of the research work in the towns of San Juan de los Lagos and Santa Ana de Guadalupe, Jalostotitlán, Mexico, on the occasion of knowing the impact it has had the policy used by the Ministry of Tourism, regarding the promotion of religious tourism in both populations as a source for economic development.Among the findings are that the project whose intent is to develop a similar route to that of the "Camino de Santiago" in Spain, and include both populations is often unknown to visitors to these religious sites.Another important result concerns the data were limited marketing of products manufactured locally or regionally. This prevents the economic benefits of religious tourism that visits this population, have very little benefit to people nearby.
Cet article propose quelques-unes des principales conclusions de la recherche menée dans les villes de San Juan de los Lagos et Santa Ana de Guadalupe, Jalostotitlán, au Mexique, sur l'impact de la politique menée par le ministère du tourisme en matière de promotion du tourisme religieux comme source potentielle de développement économique.L’accent est mis sur le projet d’une route similaire à la "Camino de Santiago" en Espagne dont le but est d’impliquer les populations dans l’accueil des visiteurs et la mise en valeur des sites religieux. Un autre résultat important concerne la commercialisation des produits fabriqués localement ou régionalement. Cela permet d'éviter les retombées économiques du tourisme profitent davantage aux villes voisines qu’aux populations locales.
El presente documento, muestras varios de los principales hallazgos del trabajo de investigación realizado en las poblaciones de San Juan de los Lagos y Santa Ana de Guadalupe, Jalostotitlán, México, con motivo de conocer el impacto que ha tenido la política utilizada por la Secretaría de Turismo, referente a la promoción del turismo religioso en ambas poblaciones como fuente generadora para su desarrollo económico.Entre los hallazgos, se encuentran que el proyecto cuya intensión es desarrollar una ruta similar a la del "camino de Santiago" en España, y que incluiría ambas poblaciones, es desconcida para los visitantes a estos sitios religiosos.Otro resultado importante, se refiere a la escaza comercialización de productos manufacturados en de manera local o regional. Este hecho impide que los beneficios económicos del turismo religioso que visita esta poblaciones, tenga muy poco beneficio para las poblaciones vecinas.
San Juan de los Lagos
Jalostotitlán
Mexique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5352
http://journals.openedition.org/etudescaribeennes/5352

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Numerical models show that small-scale convection (SSC) occurring atop a mantle plume is a plausible mechanism to rejuvenate the lithosphere. The triggering of SSC depends on the density contrast and on the rheology of the unstable layer underlying the stagnant upper part of the thermal boundary layer (TBL). Partial melting may change both properties. We analyze, using 2-D numerical simulations, how partial melting influences the dynamics of time-dependent SSC instabilities and the resulting thermo-mechanical rejuvenation of an oceanic plate moving atop of a plume. Our simulations show a complex behavior, with acceleration, no change, or delay of the SSC onset, due to competing effects of the latent heat of partial melting, which cools the plume material, and of the buoyancy increase associated with both melt retention and depletion of residue following melt extraction. The melt-induced viscosity reduction is too localized to affect significantly SSC dynamics. Faster SSC triggering is promoted for low melting degrees (low plume temperature anomalies, thick lithosphere, or fast moving plates), which limit both the temperature reduction due to latent heat of melting and the accumulation of depleted buoyant residue in the upper part of the unstable layer. In contrast, high partial melting degrees lead to a strong temperate decrease due to latent heat of melting and development of a thick depleted layer within the sublithospheric convecting layer, which delay the development of gravitational instabilities. Despite differences in SSC dynamics, the thinning of the lithosphere is not significantly enhanced relatively to simulations that neglect partial melting.
ISSN: 1525-2027

hal-01277175
https://hal.archives-ouvertes.fr/hal-01277175
DOI : 10.1002/2015GC005967

Éditeur(s) : HAL CCSD Mineralogical Society of America
Résumé : International audience
To constrain the crystallization of alkaline and volatile-rich lavas present in intraplate settings, we studied the petrological features and the geochemical composition of major, trace, and volatile elements of mineral and bulk-rock of two sodalite-bearing phonolites: (1) haüyne-plagioclase-bearing Si-K-rich phonolite from Laacher See (Germany) and (2) nosean-nepheline-bearing Si-poor phonolite from Saghro (Morocco). In haüyne-bearing phonolites (55–59 wt% SiO2, K > Na, Na+K/Al = 0.96–1.08), we found that the low silica and low sodium activity promoted the early crystallization of S-rich haüyne (13.7–13.9 wt% SO3, 0.4 wt% Cl) + S-rich apatite (0.7–0.9 wt% SO3), titanite, and rare pyrrhotite followed by clinopyroxene-plagioclase-sanidine at relatively low pressure and temperature (P = 250 MPa and T = 850 °C) and oxidized condition (ΔNNO-NNO+1, where NNO is nickel-nickel oxide buffer). The crystallization of haüyne occurred at fluid-undersaturated conditions from a silicate melt with 6 wt% H2O, 0.17–0.23 wt% Cl, 0.11–0.4 wt% S, and 0.07–0.14 wt% F. Nosean-bearing phonolites from Saghro are silica-poor and peralkaline (52–54 wt% SiO2, Na > K, Na+K/Al = 1.2) and crystallized at higher P and T (300 MPa and 950 °C) and more reduced conditions (NNO) compared to haüyne-bearing phonolites. The incongruent reaction to form nosean requires high silica and Na2O activity. The mineral assemblage and composition suggest early crystallization of nepheline followed by nosean (7.8–8.8 wt% SO3; 1–1.1 wt% Cl). The sequence of crystallization is: clinopyroxene + nepheline + S-poor apatite (<0.04 wt% SO3) + pyrrhotite followed by nosean and titanite. Nosean-bearing magmas are fluid-undersaturated with relatively low volatile content (4 wt% H2O, <0.25 wt% Cl, <0.056 wt% S, 0.08–0.1 wt% F), although Cl may have exsolved during ascent and formed a fluid phase (NaCl-bearing).Both haüyne- and nosean-bearing phonolites are last equilibrated at relatively low pressure and high temperature. Haüyne and nosean crystallized at oxidized and volatile-rich pre-eruptive conditions. They record the volatile concentrations at depth and may be used as oxybarometer. The incongruent reactions involved to form haüyne and nosean suggest that phonolitic magmas became more oxidized during crystallization. The initial volatile concentrations in basanite/nephelinite magmas, from partial melting of volatile-bearing K2O-rich mantle rock, should have been one important factor influencing the crystallization of haüyne-bearing Si-K-rich phonolite and nosean-bearing Si-poor phonolite in intracontinental setting.
ISSN: 0003-004X

hal-01278067
https://hal.archives-ouvertes.fr/hal-01278067
DOI : 10.2138/am-2015-5318

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Deep-seated gravitational spreading (DSGS) affects the slopes of formerly glaciated mountain ridges. On Mars, DSGS has played a key role in shaping the landforms of the giant Valles Marineris troughs. Though less spectacular, DSGS is common in terrestrial orogens, where understanding its mechanics is critical in the light of the ongoing climate change because it is a potential source of catastrophic landslides in deglaciated valleys. We conducted parametric numerical studies in order to identify important factors responsible for DSGS initiation. DSGS models are computed using an elastoviscoplastic finite element code. Using ADELI's software, we reproduce topographic ridge spreading under the effect of valley unloading. Two types of spreading topographic ridges are investigated, homogeneous or with horizontal rheological layering. We find that gravitational instabilities are enhanced by high slopes, which increase gravitational stress, and low friction and cohesion, which decrease yield stress. In the unlayered ridge, instability is triggered by glacial unloading with plastic strain concentration inside the ridge and at the base of the high slopes. Vertical fractures develop in the upper part of the slope, potentially leading to fault scarps. Ridge homogeneity promotes a deformation mode controlled by uphill-facing normal faulting and basal bulging. In the second case, the ridge encompasses horizontal geological discontinuities that induce rock mass anisotropy. Discontinuity located at the base of the slope accumulates plastic strain, leading to the formation of a sliding plane evolving into a landslide. The presence of a weak layer at ridge base therefore promotes another slope deformation mode ending up with catastrophic failure. Mechanical conditions and slope height being equal, these conclusions can probably be extrapolated to Earth. Compared with Mars, DSGS on Earth is inhibited because terrestrial topographic gradients are lower than in Valles Marineris, an effect counterbalanced by increased gravitational stress, where the intensity of deformation is enhanced because of the Earth gravity potential.
ISSN: 0169-555X

hal-01420007
https://hal.archives-ouvertes.fr/hal-01420007
DOI : 10.1016/j.geomorph.2016.06.011

Éditeur(s) : Elsevier Sci Ltd
Résumé : The aim of this study is to establish a baseline for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) lifetime prediction in a marine environment, by means of mechanical and physico-chemical characterization of specimens immersed in continuously renewed and filtered natural seawater in the laboratory at different temperatures (4, 25 and 40°C). Samples were also aged at sea in Lorient harbour in order to compare laboratory and natural degradation mechanisms and kinetics. Due to its morphology, hydrolysis of PHBV in natural seawater is quite slow, and samples were observed to undergo preferentially an enzymatic surface degradation. Increasing the aging temperature in the laboratory promotes the water uptake and causes hydrolysis. As two degradation mechanisms occur in parallel, the choice of test conditions is critical, and the lifetime of PHBV in a marine environment is difficult to predict accurately.
Polymer Degradation And Stability (0141-3910) (Elsevier Sci Ltd), 2014-07 , Vol. 105 , P. 237-247

Droits : 2014 Elsevier Ltd. All rights reserved.
http://archimer.ifremer.fr/doc/00188/29972/28425.pdf
DOI:10.1016/j.polymdegradstab.2014.04.026
http://archimer.ifremer.fr/doc/00188/29972/

Éditeur(s) : HAL CCSD Elsevier Academic Press
Résumé : International audience
Marines Protected Areas (MPAs) are commonly used around the world as management tools to promote the sustainable use of marine resources. It is assumed that MPAs secure human livelihoods and well-being by protecting marine ecosystems and ecosystem services. However, the links between ecological effects of MPAs and services have rarely been explored. Here we review the different impacts of MPAs on ecosystem functioning and service provision. Results highlight that empirical evidence for positive effects of MPAs on ecosystem service provision by coastal marine ecosystems is accumulating. Finally, we identified knowledge gaps on which future research efforts could focus and empower marine resource managers to make more informed decisions and maximize the value derived from their natural resource base.
Aquatic Functional Biodiversity: an Ecological and Evolutionary Perspective

ISBN : 978-0-12-417015-5
hal-01199217
https://hal-univ-perp.archives-ouvertes.fr/hal-01199217
DOI : 10.1016/B978-0-12-417015-5.00009-8

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : A development programme for the promotion of FADs in Sao Tome and Principe has implemented an original interactive approach by granting a predominant place to the initiative of the target group and its participation in decision making. The appropriation process of the innovative fads by the beneficiaries takes place in three stages described herewith. the technical research phase. This consists of formally describing an idea in reply to an expressed or potential need. The envisaged fad type is developed and tested by the project's agents in order to obtain a technically appropriate prototype.- the research and adjustment phase. The fad is given to a small number of people to be used in real-life conditions. Reaction surveys will enable its pertinence to be tested and its adaptation to normal conditions of use. This second phase results in the production of a technically and socio-economically appropriated fad. - the diffusion-perpetuation phase. This entails i) identification of the sequences of the various stages, ii) definition and grouping of contributions by the various parties, iii) production of educational auxiliaries, iv) initiating actions for user awareness and demonstration, v) staff training, vi) monitoring of the fad "socialisation", vii) solving funding problems.Original Abstract: Un projet visant, entre autres, la promotion de DCP pour la peche artisanale fonctionne a Sao Tome et Principe depuis 1993. Le processus d'appropriation daune telle innovation par les beneficiaires comprend trois etapes ici decrites. La phase de recherche technique. Il s'agit d'instrumentaliser une idee en reponse a un besoin exprime ou potentiel. L'innovation envisagee est mise au point et testee par les agents du projet en milieu controle de facon a obtenir un prototype techniquement approprie. La phase de recherche-developpement: l'outil est confie a un petit nombre de personnes pour une utilisation en conditions reelles. C'est une phase de mise au point interactive, seule susceptible de provoquer une reelle appropriation par les recipiendaires. Des enquetes de reaction permettront de tester sa pertinence et de l'adapter aux conditions normales d'utilisation. Cette seconde phase debouche sur la production d'un outil de reference approprie techniquement et socio-economiquement. La phase de diffusion-perennisation: il s'agira la : i) d'identifier les sequences d'intervention, ii) d'identifier et de regrouper les apports des differentes parties, iii) de definir et de produire les auxiliaires pedagogiques (manuels, affiches, radio), iv) d'entreprendre des actions de sensibilisation et de demonstration, v) de former les personnels, vi) de veiller a la socialisation de l'innovation, pour les outils collectifs, vii) de resoudre le probleme du financement.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15306/12641.pdf
http://archimer.ifremer.fr/doc/00042/15306/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We describe an experimental system including monitoring of temperature, pressure, pH, oxidation reduction potential and optical density at 600 nm, designed for studying the role of microorganisms on the geological sequestration Of CO2 and its transformation into solid carbonate phases. Measurements were performed in an artificial ground water (AGW) supplemented with urea (2 g.l(-1)) and equilibrated at controlled temperatures with a gaseous phase before bacterial inoculation. We used the ureolytic strain Bacillus pasteurii as a model carbonate precipitating bacteria and showed that it can successfully promote strong pH increases by ureolysis in the AGW equilibrated with CO2 pressures of up to I bar. Increasing salinities (5.8,13.5 and 35.0 g.l(-1)) have a positive effect on the rate of pH increase, whereas the effect of increasing temperatures (30,35 and 38 degrees C) is less important. Calcium is also shown to have a specific positive influence on the rate of ureolysis. The number of viable cells present in solution decreases greatly during the carbonate precipitation event but the population partially recovers once precipitation is over.
ISSN: 0009-2541

hal-00445282
https://hal.archives-ouvertes.fr/hal-00445282
DOI : 10.1016/j.chemgeo.2008.12.012

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Analogue and numerical models have shown that the strength of the lithospheric mantle controls the mode of lithosphere deformation. In extension, the presence or absence of a high strength brittle mantle respectively leads to localized or distributed rifting. However, first order geophysical data question the existence of such a brittle mantle. Here we use 2-D finite-element large strain modelling to quantify the impact of a ductile localizing mantle - instead of brittle - in triggering continental rifting. As a novelty, the mantle rheology considers the effect of grain boundary sliding during strain-induced grain size reduction, which may promote a significant strength drop and subsequent strain localization at low mantle temperature (< 700-800 °C). Our results reveal that such ductile localizing mantle implies varying modes of continental rifting that mainly depend on both the amount of weakening in the ductile mantle and the strength of the lower ductile crust. A medium to strong lower crust implies coupling between the upper crust and ductile localizing mantle, yielding to narrow continental rifting. In contrast, a weak lower crust implies decoupling between the upper crust and ductile localizing mantle, giving rise to a switch from distributed faulting at incipient strain to localized faulting at large strain. Ductile strain localization in the lithospheric mantle is therefore sufficient to trigger continental rifting, although a critical amount of weakening is required. Such ductile localizing mantle provides a relevant geological and mechanical alternative to the brittle mantle. It moreover provides a wider variety of modes of upper crustal faulting that are commonly observed in nature.
ISSN: 0040-1951

Droits : info:eu-repo/semantics/OpenAccess
insu-00924254
https://hal-insu.archives-ouvertes.fr/insu-00924254
https://hal-insu.archives-ouvertes.fr/insu-00924254/document
https://hal-insu.archives-ouvertes.fr/insu-00924254/file/Gueydan_precigout_2014_Tectonophysics.pdf
DOI : 10.1016/j.tecto.2013.11.029

Éditeur(s) : HAL CCSD
Résumé : National audience
In severe male infertility, in vitro fertilization (IVF) with intra-cytoplasmic sperm injection (ICSI) represents the sole available therapeutic option. However this technique is not always successful in promoting fertilization, as some couples completely and repeatedly fail to obtain any embryo. In many cases, this failure can be attributed to a defective rise in intracellular calcium, which is required to achieve oocyte activation. Over the last twenty years, several laboratories dedicated to assisted reproduction technologies have been using a calcium ionophore to assist oocyte activation. The aim of this review is to give an overview of the advances and consequences associated with this new technique referred to as assisted oocyte activation
Médecine Sciences: M/S

hal-01286256
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01286256
DOI : 10.1051/medsci/20163202014
PUBMED : 26936178

Résumé : The MEDECO Leg 2 cruise followed the MEDECO Leg 1 cruise that had crossed the Mediterranean Sea from the west to the east to study benthic communities of the Var canyon, deep corals found on the Calabrian Margin and cold seep ecosystems of the Mediterranean Ridge. The main objective of the MEDECO Leg 2 cruise was to study and compare, using a multidisciplinary approach, the structure and dynamics of deep-sea ecosystems at various active cold seep sites from the Nile deep sea fan and the Calabrian Arc, in the Eastern Mediterranean sea. During MEDECO Legs 1 & 2, a multiscale strategy was promoted to integrate (1) a high resolution cartography of geological structures (km2 scale), (2) a detailed mapping, including video imagery, of smaller-scale areas (scale 100 to 300 m2), (3) the physical, chemical and sedimentary characteristics of the habitat (multiscale), (4) the structure and functioning of faunal communities, including microbes, in terms of composition, diversity and biomass (multiscale), (5) small scale studies of interactions between organisms and their environment (scale < m2), (6) detailed ecophysiological studies at the organism scale on invertebrates. The use of the ROV “Victor 6000” and of various equipments for in situ and ex situ observations and measurements has helped reaching our objectives. The MEDECO cruise represented the key-Mediterranean cruise of the HERMES project “Hotspot Ecosystem Research on the Margins of European Seas” (P.I. P. Weaver, NOC-Southampton). The cruise was organized to follow the objectives of the HERMES program to better understand the functioning of deep ecosystems of the continental margins aiming at obtaining: (1) the description and quantification of the natural drivers (geological, hydrological, chemical factors) that control the distribution of ecosystems on margins; (2) the characterization and quantification of biodiversity, from bacteria to megafauna, as well as the knowledge of ecosystem functioning; (3) the study of ecosystems dynamics including the biology/physiology of key-species. Specific objectives. Colonization experiments conducted in the frame of two European programs, Diwoo and Chemeco, have given the opportunity to recover the fauna installed in the devices deployed during the Bionil cruise (2006) and to deploy new ones at the same site located in the pockmark area of the central province of Nile deep sea fan.

Droits : 2008 Insu, Cnrs, Locean
http://archimer.ifremer.fr/doc/00134/24550/22583.pdf
http://archimer.ifremer.fr/doc/00134/24550/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Analog sandbox models have been set up to study the impact of syntectonic erosion on thrust wedges with one and two decollement levels. Different accretion mechanisms are activated depending on interactions between surface processes and wedge mechanics: frontal accretion, backthrusting, underthrusting and underplating due to decollement induced duplex formation at depth. These mechanisms may function simultaneously, being located at different parts across the wedge. For all the experiments, a high friction is imposed at the base of models and the volume of eroded material remains equal to the volume of newly accreted material, maintaining a constant surface slope during the shortening. Erosion limits the forward propagation of thrust wedges and favors the underthrusting of basal layers allowing duplex formation. Erosion promotes development of major backthrusts in the thrust wedges without or with one decollement, but no backthrusts was formed in the wedges with two decollements. Slow erosion allows lower extent of basal underthrusting in comparison with regular-rate erosion. Variations in the erosion taper lead to changes in duplex geometry and exhumation rate in thrust wedges with one or two decollements. The 6 degrees erosion taper promotes formation of antiformal stack at the rear part of thrust wedge, high rate of basal underthrusting and high extent of erosional removal. The cover layers are nearly completely eroded above the antiformal stack and form the synformal klippe in frontal part of thrust wedges. The 8 degrees erosion taper allows development of individual ramp-anticlines and active forward thrusting of cover layers above the decollement and low rate of basal underplating below it, with consequent low extent of erosional removal. The results of our experiments support the observations on structural evolution and erosion in the Alberta Foothills of the Canadian Rockies.
ISSN: 0040-1951

hal-00617769
https://hal.archives-ouvertes.fr/hal-00617769
DOI : 10.1016/j.tecto.2011.01.020

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The increased expression of the Disintegrin and Metalloprotease ADAM12 has been associated with human cancers, however its role remain unclear. We have previously reported that ADAM12 expression is induced by the transforming growth factor, TGF-β and promotes TGF-β-dependent signaling through interaction with the type II receptor of TGF-β. Here we explore the implication of ADAM12 in TGF-β-mediated epithelial to mesenchymal transition (EMT), a key process in cancer progression. We show that ADAM12 expression is correlated with EMT markers in human breast cancer cell lines and biopsies. Using a non-malignant breast epithelial cell line (MCF10A), we demonstrate that TGF-β-induced EMT increases expression of the membrane-anchored ADAM12L long form. Importantly, ADAM12L overexpression in MCF10A is sufficient to induce loss of cell-cell contact, reorganization of actin cytoskeleton, up-regulation of EMT markers and chemoresistance. These effects are independent of the proteolytic activity but require the cytoplasmic tail and are specific of ADAM12L since overexpression of ADAM12S failed to induce similar changes. We further demonstrate that ADAM12L-dependent EMT is associated with increased phosphorylation of Smad3, Akt and ERK proteins. Conversely, inhibition of TGF-β receptors or ERK activities reverses ADAM12L-induced mesenchymal phenotype. Together our data demonstrate that ADAM12L is associated with EMT and contributes to TGF-β-dependent EMT by favoring both Smad-dependent and Smad-independent pathways
ISSN: 1932-6203

hal-01207281
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01207281
DOI : 10.1371/journal.pone.0139179
PUBMED : 26407179

Éditeur(s) : HAL CCSD
Résumé : Background and objectives.[br/] Dairy propionibacteria are isolated from various ecological niches, including soil, grass, silage, rumen, raw milk and dairy plants, showing great adaptability and robustness. Used as ripening starters in Swiss type cheeses, these food grade bacteria are described as nutraceutical producers and they release into the external medium short chain fatty acids (SCFA), folic acid, cobalamin and the bifidogenic 1,4- dihydroxy-2-naphthoic acid (DHNA). These compounds are known to play a pivotal role in the modulation of intestinal physiology through diet. Considering the pro-apoptotic potential of some of these metabolites, we investigated the potential of dairy products fermented by the main species, Propionibacterium freudenreichii, to prevent colon carcinogenesis. Methods.[br/] We developed a new fermented milk. Taking advantage of milk fractions, high and stable populations of P. freudenreichii were obtained in this product. It was tested in vitro on gastric HGT-1 and colic HT-29 cells with respect to the typical hallmarks of apoptosis. In vivo, the fermented milk was evaluated in piglets with respect to general health parameters and in human microbiota-associated rats, treated or not with dimethylhydrazine (DMH), with respect to intestinal proliferation and apoptosis. Results. Fermented milk induced cell death in both gastric and colon cancer cells. Cellular and molecular characterization of cells dying in response to SCFA treatment revealed cell cycle arrest, drop in intracellular ATP, depolarization of mitochondria, translocation of key apoptosis proteins, processing of caspases and fragmentation of the nucleus. Interestingly, the new fermented milk was shown to potentiate the cytotoxic effect of molecules used in cancer chemotherapy. In accordance, it modulated expression of key apoptotic proteins. In vivo, the fermented milk was evaluated in piglets. Daily oral gavage led to high colic P. freudenreichii populations, to modulation of the intestinal microbiota and modulation of intestinal cytokines. Promotion of piglets’ growth also revealed general probiotic effects of fermented milk. Considering that propionibacteria were metabolically active in the intestine, we further investigated their impact on colon carcinogenesis (induced by dimethylhydrazine, DMH) in human microbiota-associated rats. Consumption of selected strains of propionibacteria resulted in enhanced apoptotic depletion of DMH damaged epithelial cells, yet had no effect on neither proliferation nor apoptosis in healthy rats. Conclusions and perspectives.[br/] These results open new perspectives in the field of colon cancer cells prevention and/or treatment. The synergy with pro-apoptotic chemotherapy molecules suggests that such a fermented product may be proposed as a food supplement to enhance the effects of anticancer treatments.
colloque international « Nutrition and Cancer : Hot topics from biology to public health issues »
Paris, France

hal-01190452
https://hal.archives-ouvertes.fr/hal-01190452
PRODINRA : 201853

Éditeur(s) : HAL CCSD Universites de L'ouest
Résumé : International audience
The agricultural productivism which was decades ago the only prevailing model is now questioned. New perspectives are given to rural development because of the leisure and dweilling functions of the counrtyside.The F.G.R. is intended for the inhabitants professionals, and can promote employment.
Alors que le productivisme agricole qui a prévalu tout au long des décennies précédentes est remis en cause, le développement dans les campagnes des fonctions récréatives et résidentielles donne lieu à une nouvelle réflexion sur l'aménagement rural.Dans ce contexte, le Fonds de Gestion de l'Espace Rural peut constituer un outil original qu s'adresse à la fois aux usagers et aux partenaires de cet espace tout en accordant de nouvelles perspectives de rémunération aux acteurs du monde rural.
ISSN: 0029-182X

hal-01406048
https://hal.univ-antilles.fr/hal-01406048
https://hal.univ-antilles.fr/hal-01406048/document
https://hal.univ-antilles.fr/hal-01406048/file/1998%20Le%20fonds%20de%20gestion%20de%20l%27espace%20rural.pdf

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
: In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2'-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.
ISSN: 0953-816X

inserm-00866212
http://www.hal.inserm.fr/inserm-00866212
DOI : 10.1111/ejn.12348
PUBMED : 23981075

Éditeur(s) : HAL CCSD American Association for Cancer Research
Résumé : International audience
Homing of CD8+ T lymphocytes to the tumor microenvironment is an important step for mounting a robust antitumor immune response. TGF-β is responsible for CD103 (αEβ7) integrin induction in activated intraepithelial CD8+ T lymphocytes. However, the interplay between TGF-β and CD103 and their contribution to T-cell infiltration and antitumor activity remain unknown. Here, we used viable human lung tumor slices and autologous tumor antigen-specific T-lymphocyte clones to provide evidence that CD103 is directly involved in T-lymphocyte recruitment within epithelial tumor islets and intratumoral early T-cell signaling. Moreover, TGF-β enhanced CD103-dependent T-cell adhesion and signaling, whereas it inhibited leukocyte function-associated antigen (LFA)-1 (αLβ2) integrin expression and LFA-1-mediated T-lymphocyte functions. Mechanistic investigations revealed that TGF-β bound to its receptors (TGFBR), which promoted the recruitment and phosphorylation of integrin-linked kinase (ILK) by TGFBR1. We further show that ILK interacted with the CD103 intracellular domain, resulting in protein kinase B (PKB)/AKT activation thereby initiating integrin inside-out signaling. Collectively, our findings suggest that the abundance of TGF-β in the tumor microenvironment may in fact engage with integrin signaling pathways to promote T-lymphocyte antitumor functions, with potential implications for T-cell-based immunotherapies for cancer
ISSN: 0008-5472

Droits : info:eu-repo/semantics/OpenAccess
hal-01282442
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01282442
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01282442/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01282442/file/TGF%CE%B2%20Signaling%20Intersects%20with%20CD103-boutet2016-final.pdf
DOI : 10.1158/0008-5472.CAN-15-1545