Éditeur(s) : SEG : Société des Économistes de la Guadeloupe Université des Antilles et de la Guyane
Description : Terrain d'expérimentation de la colonisation et de la mondialisation, la Caraïbe vit une difficile adaptation à l'essor du capitalisme mondial. Elle est ainsi à la recherche d'un nouveau rôle et d'une nouvelle place dans l'économie monde.
Siècle(s) traité(s) : 20
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V13115
V13115

Résumé : Additional label information: Sorghum seedlings after transplanting from seed crop to field. This is one of the seed groups collected by Dr. Brandes in Ethiopia in the winter of 1944. The old stalks of the previous crops are used as a guard against erosion on this sloping land. (#52A) Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C. Z. 88

Résumé : Additional label information: leaves, fruit and flower. Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C. Z. 5

Éditeur(s) : Elsevier
Résumé : The New Caledonia blue shrimp Litopenaeus stylirostris is commercially produced under semi-intensive rearing conditions. The size of the farming earthen ponds (5–10 ha) and the subsequent production constraints make it difficult to use them as experimental units for random experiments. Moreover, since every pond has its own characteristics, ponds' effects cannot be ruled out, thus making it hard to define true replicates. In order to design future experiments under conditions resembling those used in production, we evaluated the possible use of floating cages as experimental units with the aim of assessing treatment effects with a reasonable statistical power. To this end, two sets of floating cages were placed respectively in two different ponds in a commercial farm. In both cases the zootechnical conditions in the cages were similar in post-larvae origin, management, and diet and feeding regime. The aim of the study was to evaluate on a technical basis the possibility of rearing shrimps in floating cages set up in earthen ponds and to assess this method from a statistical standpoint. Shrimps reared in and outside the cages showed comparable growth and survival rates. The variability in the zootechnical parameters between cages, expressed as the estimated coefficient of variation (CV) for survival rate, total final biomass (g), final body weight (g), daily increment in body weight (g day− 1) and FCR were 11.0, 13.7, 4.4, 5.4, and 7.0%, respectively. On the basis of these figures, we calculated for a given statistical power (80%) the number of replicates (cages) that would be required to reveal significant differences between two treatments, at a 5% level of significance. We found that for expected differences of 20% from the control mean, 3 and 6 floating cages per treatment would be reasonable to determine statistical differences for growth parameters and survival rate, respectively. Moreover, we showed a significant pond effect in regard to survival and growth between the two sets of cages. These results illustrated the within-farm variability among the ponds, and confirmed that the specific characteristics of each pond from the same farm make it difficult to use the ponds themselves as experimental units. The study demonstrates that rearing in floating cages is an economical, powerful and sensitive experimental tool for shrimp culture studies specifically carried out under conditions close to semi-intensive production.
Aquaculture (0044-8486) (Elsevier), 2008-07 , Vol. 279 , N. 1-4 , P. 63-69

Droits : 2008 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2008/publication-4363.pdf
DOI:10.1016/j.aquaculture.2008.03.053
http://archimer.ifremer.fr/doc/00000/4363/

Résumé : Additional label information: fruit. Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C.Z. 58

Éditeur(s) : EUTIC : le réseau international et interdisciplinaire pour les Enjeux et Usages des Technologies de l'Information et de la Communication
Extrait de : "Les écosystèmes numériques et la démocratisation informationnelle" : colloque, les 3 et 4 novembre 2015. Université des Antilles
Description : Caroline Ladage et Christine Poplimont, Maître de Conférences et Professeur de l'université d'Aix-Marseille présentent une expérimentation réalisée dans l'enseignement supérieur pour mesurer les effets de la construction de cartes conceptuelles collaboratives sur les apprentissages dans le champ de l'éducation au développement durable. La communication a également une visée compréhensive de la contribution de ce type de dispositif dans une perspective plus large, de partage de contenus d'enseignement dans un contexte pluridisciplinaire et de travail collaboratif à distance. Le dispositif de recherche est basé sur un double recueil de données : les traces de l'activité avec l'analyse des cartes elles-mêmes, ainsi que les résultats d'un questionnaire auprès des auteurs des cartes. Les résultats attendus devraient mettre en évidence une diversité de perceptions du travail avec ce type de dispositif, appelant à repenser les ingénieries de formation les mettant en jeu.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15402
V15402

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C. Z. 43

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation Caribbean Food Crops Society (CFCS)
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Farmers are very inventive, but their innovations often remain confined to their farm, or to small local networks. And the interest they might have for farmers other than their inventors is seldom analyzed. Tracking on-farm innovations aims at finding technical or organizational innovations designed by farmers, to characterize their agronomic, economic and environmental performance and analyze the conditions in which this performance is expressed. The talk presents the various stages of this tracking approach, applied to innovative cropping systems, basing on a variety of recent works, in France, Argentine, Burkina Faso and China: (1) Defining what we are looking for; (2) Identifying innovative systems; (3) Describing and characterizing innovative systems; (4) Assessing innovative systems; (5) Specifying the conditions for the success of innovative systems. The results of the tracking are aimed not only at the farmers, but at their advisors and researchers too: (i) Innovative and effective cropping systems, which can be a source of inspiration for farmers; (ii) Confirmation of the interest of a principle for reasoning cropping systems; (iii) Innovative practices, analyzed within a systemic framework, which can serve as bricks for the design of new systems; (iv) Questions to be looked into more thoroughly by experimentation. Compared to traditional approaches in agronomy, tracking innovative cropping systems provides a double break: break with a top-down approach, where agricultural R & D is regarded as the only source of innovation: by mobilizing the innovative capacities of farmers, tracking increases our collective capacity to invent new practices or new cropping systems, by using not only technical and scientific knowledge, but also the empirical knowledge, that is so rich in agriculture. And break with the primacy given to experimentation, as a source for the production of knowledge and the assessment of innovations. Les agriculteurs sont très inventifs, mais leurs innovations demeurent souvent confinées à leur ferme, ou à de petits réseaux locaux. Et l'intérêt qu'elles pourraient avoir pour des agriculteurs autres que leurs inventeurs est rarement analysé. Le cheminement des innovations à la ferme vise à trouver les innovations techniques ou organisationnelles conçues par des agriculteurs, pour caractériser leur agronomique, économique et la performance environnementale et pour analyser les conditions en lesquelles ces résultats sont exprimés. L'entretien présente les diverses étapes de cette approche de cheminement, appliquées aux systèmes d'emblavage innovateurs, basant sur un grand choix de travaux récents, les Frances, l'Argentin, au Burkina Faso et en Chine : (1) définissant ce que nous recherchons ; (2) identification des systèmes innovateurs ; (3) description et caractérisation des systèmes innovateurs ; (4) l'évaluation des systèmes innovateurs ; (5) spécification des conditions pour le succès des systèmes innovateurs. Les résultats du cheminement sont visés non seulement les agriculteurs, mais leurs conseillers et chercheurs trop : (i) systèmes d'emblavage innovateurs et efficaces, qui peuvent être une source d'inspiration pour des agriculteurs ; (ii) confirmation d'intérêt d'un principe pour les systèmes d'emblavage de motif ; (iii) pratiques innovatrices, analysées dans un cadre systémique, qui peut servir de briques à la conception de nouveaux systèmes ; (iv) questions à regarder dans plus complètement par expérimentation. Comparé aux approches traditionnelles en agronomie, le cheminement des systèmes d'emblavage innovateurs fournit une double coupure : cassez avec une approche hiérarchisée, où la R&D agricole est considérée comme la seule source d'innovation : en mobilisant les capacités innovatrices d'agriculteurs, le cheminement augmente notre capacité collective d'inventer de nouvelles pratiques ou nouveaux systèmes d'emblavage, à l'aide non seulement de technique et de la connaissance scientifique, mais également la connaissance empirique, qui est si riche en agriculture. Et coupure avec la primauté donnée à l'expérimentation, comme source pour la production de la connaissance et de l'évaluation des innovations.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16242
V16242

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C. Z. 18

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The equilibrium phase relations of a mafic durbachite (53 wt.% SiO2) from the TA (TM) ebi pluton, representative of the Variscan ultrapotassic magmatism of the Bohemian Massif (338-335 Ma), have been determined as a function of temperature (900-1,100A degrees C), pressure (100-200 MPa), and H2O activity (1.1-6.1 wt.% H2O in the melt). Two oxygen fugacity ranges were investigated: close to the Ni-NiO (NNO) buffer and 2.6 log unit above NNO buffer (a dagger NNO + 2.6). At 1,100A degrees C, olivine is the liquidus phase and co-crystallized with phlogopite and augite at 1,000A degrees C for the whole range of investigated pressure and water content in the melt. At 900A degrees C, the mineral assemblage consists of augite and phlogopite, whereas olivine is not stable. The stability field of both alkali feldspar and plagioclase is restricted to low pressure (100 MPa) at nearly water-saturated conditions (< 3-4 wt.% H2O) and T < 900A degrees C. A comparison between experimental products and natural minerals indicates that mafic durbachites have a near-liquidus assemblage of olivine, augite, Ti-rich phlogopite, apatite and zircon, followed by alkali feldspar and plagioclase, similar to the mineral assemblage of minette magma. Natural amphibole, diopside and orthopyroxene were not reproduced experimentally and probably result from sub-solidus reactions, whereas biotite re-equilibrated at low temperature. The crystallization sequence olivine followed by phlogopite and augite reproduces the sequence inferred in many mica-lamprophyre rocks. The similar fractionation trends observed for durbachites and minettes indicate that mafic durbachites are probably the plutonic equivalents of minettes and that K- and Mg-rich magmas in the Bohemian Massif may have been generated from partial melting of a phlogopite-clinopyroxene-bearing metasomatized peridotite. Experimental melt compositions also suggest that felsic durbachites can be generated by simple fractionation of a more mafic parent and mixing with mantle-derived components at mid crustal pressures.
ISSN: 0010-7999

hal-00618418
https://hal.archives-ouvertes.fr/hal-00618418
DOI : 10.1007/s00410-009-0430-5

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C.Z. 93

North America -- Panama -- Caribbean
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C.Z. 64

Résumé : Additional label information: Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C. Z. 69

Résumé : Additional label information: Furcraea esbuya var. integra is characterized by its smooth, spineless leaves. Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C. Z. 6

Résumé : Additional label information: Flowering branch. Summit, Canal Zone. USDA - Foreign Agric'l Relations Photo
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C. Z. 63

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C.Z. 60

Éditeur(s) : University of Florida, Agricultural Experiment Station University of Florida, Agricultural Experiment Station ( Gainesville, Fla. )
Résumé : (Statement of Responsibility) A.J. Norden, H.A.D. Chesney, A.P. Stephenson.
(Internal Comments) A.J. Norden, H.A.D. Chesney, A.P. Stephenson.
Cover title.
January 1972.
University of Florida Agricultural Experiment Station circular S-215
Guyana
Guyana

23904063