Éditeur(s) : Amer Soc Microbiology
Résumé : A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture.
Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 1999-03 , Vol. 65 , N. 3 , P. 1145-1151

Droits : American society for microbiology
http://archimer.ifremer.fr/doc/1999/publication-2734.pdf
http://archimer.ifremer.fr/doc/00000/2734/

Éditeur(s) : Elsevier
Résumé : The blue shrimp Litopenaeus stylirostris was exposed to pond sediment for 48 h and its physiological response was studied in relation to molt stage. Shrimp were maintained in cages within a semi-intensive pond after 4 months of rearing. Hypoosmoregulatory capacity (hypo-OC), magnesium ions (Mg ions), glucose, total proteins and oxyhaemocyanin were assayed in haemolymph. An increase of 370% to 500% in Mg ions concentration and 200% to 266% in glucose concentration, and a decrease in hypo-OC in shrimp maintained close to the centre of the pond indicated a short-term stress response in all molt stages. Indicators of water and sediment pore water quality were within normal operating limits, however this area was characterized by high water content and relatively high total ammonia nitrogen in pore water of sediment. To a lesser extent, shrimp maintained in two other stations also presented a stress response. Physiological response of shrimp in molt stage D, compared to C/D-0 and D-2 seems to indicate that shrimp were more sensitive to environment at this stage. The use and choice of physiological parameters to detect shrimp stress in a pond are discussed.
Aquaculture (0044-8486) (Elsevier), 2006-03 , Vol. 253 , N. 1-4 , P. 703-711

Droits : 2005 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2006/publication-1675.pdf
DOI:10.1016/j.aquaculture.2005.09.008
http://archimer.ifremer.fr/doc/00000/1675/

Résumé : The New-Caledonian shrimp industry is based on the controlled reproduction of the shrimp Litopenaeus stylirostris, a species which was introduced in the 80s. The major difficulty to which the industry has been faced for 10 years is the occurrence of the "syndrome 93", which corresponds to mortality phases when the temperature falls down in April-May-June. This mortality is associated to the pathogenic bacteria Vibrio penaecida and is expressed at different levels which are variable from year ta year and from pond to pond. No resistance to this pathology has been developed spontaneously. This is likely due to the protocole used to rear spawners, which does not allow to implement an efficient selective pressure at each generation An experimental selection on the criteria of survival after picks of syndrome 93 has been conducted at the Laboratoire Aquacole de Calédonie. The 3rd selected generation demonstrates survival rates improved by 20% during experimental infections with V. penaeicida in comparison with a non selected control population of same genetic origin. The comparison of the correlated responses on the level of expression of 5 genes which are potentially implicated in immunity phenomena (Peneidins, lysozyme, transglutaminase. profiline, annexine) shows that the selected population has a level of expression in lyzozyme twice higher than the control population. This result suggests that the lysozyme could be a genetic marker which could be used in a selective breeding program to he developed in relation with the private hatcheries.
La filière crevette de Nouvelle-Calédonie repose sur la maîtrise de la reproduction contrôlée de la crevette Litopenaeus stylirostris, espèce introduite dans les années 80. La difficulté majeure que rencontre la filière depuis une dizaine d'années est la récurrence du « syndrome 93 », qui correspond à des épisodes de mortalités lors des baisses de température en avril-mai-juin. Ces mortalités sont associées à la bactérie pathogène Vibrio penaeicida et s'expriment à des niveaux d'intensité variable d'une année à l'autre et d'un bassin à l'autre. Aucune résistance vis-à-vis de cette pathologie ne s'est développée spontanément. Ceci est vraisemblablement lié au protocole employé pour l'élevage des géniteurs qui ne permet pas d'exercer une pression de sélection efficace à chaque génération. Une expérience de sélection sur un critère de survie à des épisodes de syndrome 93 a été menée au Laboratoire Aquacole de Calédonie. La 3ème génération sélectionnée montre des survies améliorées de l'ordre de 20% lors d'infections expérimentales à V. penaeicida par rapport à une population témoin non sélectionnée de même origine génétique. La comparaison des réponses corrélées sur le niveau d'expression de 5 gènes potentiellement impliqués dans les phénomènes de défense immunitaire (penaeidine, lysozyme, transglutaminase, profiline, annexine) montre que la population sélectionnée a un niveau d'expression en lysozyme deux fois plus élevé que la population témoin. Ce résultat suggère que le lysozyme pourrait être un marqueur génétique utilisable dans un programme de sélection à développer en relation avec les écloseries de production.

Droits : 2003 Ifremer
http://archimer.ifremer.fr/doc/00109/22020/19643.pdf
http://archimer.ifremer.fr/doc/00109/22020/

Éditeur(s) : 8 th Workshop of World Mariculture Society, Costa Rica, 10-13 janvier 1977
Résumé : To develop shrimp fanning in French Polynesia where no indigenous commercial species of penaeid prawn exists, the first step is to select the right species according to the following criteria: maturation and spawning in captivity, mass production of post-larvae, fast growth at semi-intensive density, acceptability of artificial diet, disease resistance and hardiness. This paper presents the results on P. monodon, Fabricius, the largest Indo-Pacific species. Experiments have been conducted in the "Centre Océanologique du Pacifique" in Tahiti Island where the environmental conditions are water temperature range 25-29 C, salinity 35 ppt, pH 8.2. [NOT CONTROLLED OCR]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1977/acte-4412.pdf
http://archimer.ifremer.fr/doc/00000/4412/

Éditeur(s) : Elsevier Science Bv
Résumé : The euryhaline white shrimp Litopenaeus vannamei lives in both coastal and oceanic areas through ontogeny. Its osmoregulation pattern and variations in its tolerance to salinity are partially known from several studies under different experimental conditions (developmental stages, salinities and acclimation procedures). Although L. vannamei is recognized as one of the most euryhaline penaeid species, with adults and juveniles exhibiting an hyper-hypo-osmoregulatory pattern and being able to tolerate a wide salinity range, little is known on larval and early postlarval strategies to cope with salinity fluctuations. In order to establish their euryhalinity range and to fully understand the ontogenetic changes in L. vannamei osmoregulatory pattern, we evaluated the effect of six salinities (5, 10, 20, 32, 45 and 60 psu) on 17 developmental stages by directly exposing them to experimental salinities and conducting observations during the next 48 hours. At 5 hours post osmotic shock (hps), all developmental stages survived (> 20%) in 20, 32 and 45 psu. The euryhalinity and osmoregulation pattern changed at some developmental stages of L. vannamei. The hyper-hypo osmoregulatory pattern exhibited by Juvenile and Adult appears to be established early in the first post-larval stage PL1 (ontogenetic osmoregulation pattern type 3), with higher tolerance to salinity variations observed in PL2, PL4 and PL22 suggesting that L. vannamei shows a progressive increase in the efficiency of osmoregulatory mechanism following last metamorphosis.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-02 , Vol. 422 , P. 261-267

Droits : 2013 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00169/28068/26283.pdf
DOI:10.1016/j.aquaculture.2013.11.034
http://archimer.ifremer.fr/doc/00169/28068/

Résumé : This report is presented by Chantal Cahu for obtaining accreditation to supervise research. Since 1981, her research activity has addressed the study of reproducers' nutrition and the young stages of development of marine organisms belonging to two different branch lines, crustaceans and fish. This work, performed in a base-lined research body, IFREMER, is placed within a more general programme of aquaculture development. Indeed, whatever species are at issue, the development of aquaculture in France, as at the global level, is based on the production of juveniles in hatcheries. My work has therefore more precisely the goal of acquiring knowledge about the nutritional needs, mechanisms of digestion and the development of these animals in order to improve the production of prawn post-larvae and fish alevin in the hatchery. This work took place in three main periods that appear in this report: - The study of reproducers' nutrition and its influence on the quality of the Penaeid prawn's eggs and larvae. My work has focused mainly on the effect of lipids and vitamins on the development of eggs and larvae. - The study of the ontogenesis of the digestive functions and the nutritional needs of sea fish larvae, with bass as the model. The goal was to determine the specific nutritional needs of the young stages by studying the implementation and regulation of pancreatic and intestinal digestive enzymes. - The study of the regulation of certain developmental genes by means of nutrients, so as to understand how these nutrients behave during ontogenesis, particularly during development of the skeleton.
Ce mémoire est présentée par Chantal Cahu pour pour l'obtention d'une habilitation a diriger des recherches. Depuis 1981, son activité de recherche concerne l'étude de la nutrition des reproducteurs et des jeunes stades de développement d'organismes marins appartenant à deux embranchements différents, les crustacés et les poissons. Ce travail réalisé dans un organisme de recherche finalisée, l'Ifremer, se situe dans un programme plus général de développement de l'aquaculture. En effet, quelles que soient les espèces en jeu, le développement de l'aquaculture en France comme au niveau mondial repose sur la production de juvéniles en écloserie. Mon travail a donc plus précisément pour objectif l'acquisition de connaissances sur les besoins nutritionnels, les mécanismes de digestion et le développement des ces animaux afin d'améliorer la production de post-larves de crevettes et d'alevins de poissons en écloserie. Ce travail s'est déroulé en trois grandes périodes qui apparaissent dans ce mémoire : - L'étude de la nutrition des reproducteurs et son influence sur la qualité des oeufs et des larves de crevettes Pénéides. Mon travail a essentiellement porté sur l'effet des lipides et des vitamines sur le développement des oeufs et des larves. - L'étude de l'ontogenèse des fonctions digestives et les besoins nutritionnels des larves de poissons marins, avec le bar comme modèle. Le but était de déterminer les besoins nutritionnels spécifiques des jeunes stades, en étudiant la mise en place et la régulation des enzymes digestives pancréatiques et intestinales. - L'étude de la régulation de certains gènes du développement par les nutriments, de façon à comprendre comment ces nutriments agissent lors de l'ontogenèse, notamment lors du développement du squelette.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2006/rapport-2086.pdf
http://archimer.ifremer.fr/doc/00000/2086/

Éditeur(s) : 10 th Meeting of the World Mariculture Society. Honolulu, January 1979
Résumé : Three shrimp species, Penaeus monodon, P. stylirostris (Panama and Mexican strains) and P. vannamei have matured and spawned in captivity at the Centre Océanologique du Pacifique (COP) laboratory. The shrimp, originally stocked as juveniles or postlarvae, were reared to adult size in tanks and ponds and have completed the cycle: F3 generation for P. monodon, F2 for P. stylirostris (Panama strain) and P. vannamei and F1 for P. stylirostris (Mexican strain). Maturation occurs naturally under our rearing conditions or is induced by unilateral eyestalk ablation. Details are given on mating behavior, ovarian development, number of spawnings, egg viability and maintenance of captive brood stock. Partial results so far obtained for P. monodon and P. stylirostris clearly show a reared brood stock could sustain a commercial hatchery. [NOT CONTROLLED OCR]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1979/acte-4423.pdf
http://archimer.ifremer.fr/doc/00000/4423/

Éditeur(s) : Blackwell science
Résumé : The prawn Penaeus stylirostris (Stimpson), when fed for 28 days with n-3 highly unsaturated fatty acid (HUFA)-enriched feed pellets, demonstrated an enhanced resistance to variations in environmental parameters (a decrease in temperature and salinity over a 4-day period from 28 degreesC to 17 degreesC and from 35 parts per thousand to 10 parts per thousand respectively) and an improvement in their immune defence potential, i.e. increased agglutination titre of plasma and increased respiratory burst of haemocytes.
Aquaculture Research (1355-557X) (Blackwell science), 2001-02 , Vol. 32 , N. 2 , P. 91-94

Droits : 2001 Blackwell
http://archimer.ifremer.fr/doc/2001/publication-606.pdf
DOI:10.1046/j.1365-2109.2001.00534.x
http://archimer.ifremer.fr/doc/00000/606/

Éditeur(s) : Elsevier
Résumé : The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied. Mortality and OC were studied in relation to molt stages. Half of the shrimps died after respectively 100 min at 0.5 mg O-2 l(-1) at 28 degreesC and 153 min at 0.4 mg O-2 l(-1) at 22degreesC. Mortality was significantly higher in stages D-2 and B compared to stages C and Do. Control shrimps in stage D2 had a lower hypo-OC than shrimps in stages C and Do both at 28 and 22 degreesC. Temperature decrease had no effect on hypo-OC. Hypoxia reduced hypo-OC for all stages, whatever the temperature, but the effect was greater in stage C at 27 degreesC (68% decrease) compared to 22 degreesC (49%) and stage D-2 at both temperatures (respectively, 43% and 58% at 27 and 22 degreesC). Combination of temperature and hypoxia had a significant effect on hypo-OC. Low temperature reduced the effect of hypoxia presumably by slowing down the metabolism. Results are discussed in relation to pond observation. (C) 2004 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier), 2005-02 , Vol. 244 , N. 1-4 , P. 315-322

Droits : 2004 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2005/publication-372.pdf
DOI:10.1016/j.aquaculture.2004.11.010
http://archimer.ifremer.fr/doc/00000/372/

Éditeur(s) : Elsevier Science Bv
Résumé : Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical condition of shrimp did not specifically allow us to foresee disease outbreak; (d) shrimp at late premolt stage D-2 and early postmolt stage A appeared to be at some points of the mortalities - but not continuously - the most sensitive to disease; (e) physiological, immunological and nutritional parameters of uninfected shrimp in the DF pond were altered, suggesting that environmental stress occurred just before the first mortalities; (f) data suggest that Vn-infected shrimp are more stressed than the presumed healthy shrimp. Combined with pathological and environmental knowledge gained in parallel during this survey, a conceptual model is proposed. Results suggest that an unstable environment induced conditions (i) stressful for the shrimp, increasing their susceptibility to bacterial infections and (ii) favoring the proliferation of the pathogen in the pond. The combination of these two processes could lead to significant mortality.
Aquaculture (0044-8486) (Elsevier Science Bv), 2013-04 , Vol. 388 , P. 105-114

Droits : 2013 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00176/28764/27567.pdf
DOI:10.1016/j.aquaculture.2013.01.010
http://archimer.ifremer.fr/doc/00176/28764/

Éditeur(s) : Elsevier
Résumé : Penaeid shrimp reared in earthen ponds are exposed to sediment, which can, in some instances, induce a stress. In seawater, the osmoregulatory capacity (hyporegulation) is a useful tool to compare the physiological condition of shrimp exposed to various stressors. By keeping some shrimp in cages at different locations of a single pond heterogeneous in terms of sediment quality, it was possible, using osmotic pressure (OP), to identify some locations where the stress was maximum and some others where it was minimum. Simultaneously, sediment samples were taken and analysed in order to evaluate some physico-chemical parameters that could be related to the stress observed in the shrimps kept in the cages. This approach allowed to show a significant positive correlation between the pH of the sediment surface and the shrimp osmotic pressure. This result was confirmed in a study carried out in experimental 70-1 tanks, where osmotic pressure decreased significantly as water pH decreased from 7.0 to 6.5. The methodology developed in this study may be useful to evaluate the stress caused by sediment in shrimp farms. (C) 2004 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier), 2004-10 , Vol. 240 , N. 1-4 , P. 297-312

Droits : 2004 Elsevier
http://archimer.ifremer.fr/doc/2004/publication-1866.pdf
DOI:10.1016/j.aquaculture.2004.07.001
http://archimer.ifremer.fr/doc/00000/1866/

Éditeur(s) : Elsevier
Résumé : A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp vibriosis "Syndrome 93". These results highlight that the "Summer Syndrome" is most probably caused by an emergent clonal pathogen that therefore deserves surveillance and that AP-PCR can satisfactorily be used for that purpose
Systematic and Applied Microbiology (0723-2020) (Elsevier), 2006-11 , Vol. 29 , N. 7 , P. 570-580

Droits : 2005 Elsevier
http://archimer.ifremer.fr/doc/2006/publication-1913.pdf
DOI:10.1016/j.syapm.2005.12.005
http://archimer.ifremer.fr/doc/00000/1913/