Manioc : Portail | resultats 1 - 20 pour la recherche: Buis

Almanach royal, année bissextile M.DCC.LXXXVIII présenté à sa majesté pour la première fois en 1699
Auteur(s) : Houry, Laurent d' (1644-1725)
Auteurs secondaires : Debure, François-Jean-Noël (1743-1802)
Année de publication :

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Le commerce des isles françoises de l'Amérique par Marseille et autres ports du royaume
Auteur(s) : Chambon (17..-17.. ; receveur général des finances)
Année de publication :

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Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Auteur(s) : Lallias, Delphine Arzul, Isabelle Heurtebise, Serge Ferrand, Sylvie Chollet, Bruno Robert, Maeva Beaumont, Andrew Boudry, Pierre

Éditeur(s) : Physiomar 08 Physilogical aspects of reproduction, nutrition and growth "Marine molluscs in a changing environment"
Résumé : Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to B. ostreae. The first infections occurred after 4 months of challenge in the tested oysters (Family 1 and Family 2). The cumulative mortalities after 5 monts were 58% for the wil oysters, 9% for Family 1 (20-month old at the beginning of the experiment) and 20% for Family 2 (8-month, old). The parasite could be detected in 66.8% of the dying wild oysters, 67.5% of the dying oysters of Family 1, 89% of the dying oysters of Family 2 and only 11% of the surviving oysters of Family 2. The mortality was significantly higher in Family 2 thant in Family 1 (x2= 20.87, p<0.001, d.f.) as well as the level of infection by the parasite found in heart smear (x2=24.34, p<0.001, 4 d.f.). This result demonstrates that prespawning oysters as yong as 1 year-old can become infected with the parasite and die from bonamiosis. This result is inconsistent with the commonly accepted critical age of 2 years-old for the disease development. The most probable cause of the dscrepancy in the development of bonamiosis between the 2 tested families is a difference in genetic background.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2008/acte-4535.pdf
http://archimer.ifremer.fr/doc/00000/4535/

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Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana
Auteur(s) : Tirera, Sourakhata Ginouves, Marine Donato, Damien Caballero, Ignacio S. Bouchier, Christiane Lavergne, Anne Bourreau, Eliane Mosnier, Emilie
Auteurs secondaires : Institut Pasteur de la Guyane Française ; Institut Pasteur de la Guyane - Réseau International des Instituts Pasteur (RIIP) Epidemiologie des Parasitoses Tropicales (EPaT Team) ; Université des Antilles et de la Guyane (UAG) - Institut National de la Santé et de la Recherche Médicale (INSERM) Centre Hospitalier de Cayenne Andrée Rosemon Boston University [Boston] (BU) Génomique (Plate-Forme) ; Institut Pasteur [Paris] Centre Hospitalier de Cayenne Andrée Rosemon Centre Hospitalier de l'Ouest Guyanais Département de Virologie ; Institut Pasteur [Paris]

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
IntroductionLeishmania RNA virus type 1 (LRV1) is an endosymbiont of some Leishmania (Vianna) species in South America. Presence of LRV1 in parasites exacerbates disease severity in animal models and humans, related to a disproportioned innate immune response, and is correlated with drug treatment failures in humans. Although the virus was identified decades ago, its genomic diversity has been overlooked until now.Methodology/Principles findingsWe subjected LRV1 strains from 19 L. (V.) guyanensis and one L. (V.) braziliensis isolates obtained from cutaneous leishmaniasis samples identified throughout French Guiana with next-generation sequencing and de novo sequence assembly. We generated and analyzed 24 unique LRV1 sequences over their full-length coding regions. Multiple alignment of these new sequences revealed variability (0.5%±23.5%) across the entire sequence except for highly conserved motifs within the 5' untranslated region. Phylogenetic analyses showed that viral genomes of L. (V.) guyanensis grouped into five distinct clusters. They further showed a species-dependent clustering between viral genomes of L. (V.) guyanensis and L. (V.) braziliensis, confirming a long-term co-evolutionary history. Noteworthy, we identified cases of multiple LRV1 infections in three of the 20 Leishmania isolates.Conclusions/SignificanceHere, we present the first-ever estimate of LRV1 genomic diversity that exists in Leishmania (V.) guyanensis parasites. Genetic characterization and phylogenetic analyses of these viruses has shed light on their evolutionary relationships. To our knowledge, this study is also the first to report cases of multiple LRV1 infections in some parasites. Finally, this work has made it possible to develop molecular tools for adequate identification and genotyping of LRV1 strains for diagnostic purposes. Given the suspected worsening role of LRV1 infection in the pathogenesis of human leishmaniasis, these data have a major impact from a clinical viewpoint and for the management of Leishmania-infected patients.
ISSN: 1935-2727

pasteur-01583523
https://hal-riip.archives-ouvertes.fr/pasteur-01583523
DOI : 10.1371/journal.pntd.0005764
PUBMED : 28715422

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